Biomaterials Lab 3: Spectroscopy of Hydrogels
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Questions and Answers

What is the main purpose of FTIR spectroscopy?

  • To observe the mechanical properties of materials.
  • To analyze the color properties of compounds.
  • To study the interaction of infrared light with matter. (correct)
  • To measure the temperature of a sample.

What happens when the frequency of IR light matches one of the vibrational frequencies of a compound?

  • The compound emits light.
  • The compound absorbs the IR light. (correct)
  • The compound cools down.
  • The compound becomes solid.

In FTIR spectroscopy, what is the next step after detecting the IR light that has passed through the sample?

  • Plotting the information to create an IR spectrum. (correct)
  • Changing the temperature of the sample.
  • Calculating the mass of the sample.
  • Creating an image of the sample.

What is the role of Fourier-Transform (FT) in FTIR spectroscopy?

<p>To produce the intensity as a function of wavenumber. (B)</p> Signup and view all the answers

What does ATR stand for in ATR FTIR spectroscopy?

<p>Attenuated Total Reflectance. (B)</p> Signup and view all the answers

What is the penetration depth of the evanescent wave in ATR FTIR spectroscopy?

<p>0.5-2 μm. (B)</p> Signup and view all the answers

How does light travel according to the principles of spectroscopy?

<p>Like a wave that can spread out. (B)</p> Signup and view all the answers

Which of the following statements about the intensity of the field in ATR FTIR spectroscopy is true?

<p>The intensity of the field decays rapidly. (D)</p> Signup and view all the answers

Flashcards

FTIR Spectroscopy

A technique that studies the interaction of infrared light with matter to identify chemical bonds and molecular structures.

Infrared Spectroscopy

The study of how infrared light interacts with molecules.

Vibrational frequencies

Specific frequencies of vibration for each chemical bond and molecular structure.

Attenuated Total Reflectance (ATR) FTIR Spectroscopy

A technique that analyzes solid or liquid samples directly, without sample preparation, by transmitting IR light through a crystal.

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Evanescent wave

The light that interacts with the sample in ATR FTIR.

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IR spectrum

A plot of the intensity of IR light absorbed by a sample as a function of wavenumber, produced from the FT.

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Wavenumber

A unit of measurement used in spectroscopy, representing the frequency of the light.

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Fourier Transform (FT)

A mathematical process to transform the signal, converting the raw data into the intensity as a function of wavenumber.

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Study Notes

Course Information

  • Course title: Biomaterials
  • Course code: ENGR-UH 4810
  • Professor: Jeremy Teo
  • Instructor: Shafiya Sabah
  • Semester: Fall 2024
  • Institution: NYU Abu Dhabi

Lab 3: Spectroscopy-Based Characterization of Hydrogels

  • Focuses on using spectroscopy to examine hydrogels.

FTIR Spectroscopy (Fourier Transform Infrared Spectroscopy)

  • Definition: Infrared spectroscopy examines the interaction of infrared light with matter.
  • Range: Infrared light spans from 4000 cm⁻¹ to 400 cm⁻¹. Different regions are categorized (near, mid, far).
  • Mechanism: Atoms in compounds vibrate. Specific vibrational frequencies correspond to chemical bonds and molecular structure. When IR light frequency matches a vibration, the molecule absorbs it, exciting the vibration.
  • Analysis: IR light passes through the sample, and the absorbed frequencies are analyzed to build an infrared spectrum.
  • Fourier Transformation (FT): The signal is transformed to produce intensity as a function of wavenumber.

FTIR Spectroscopy Functional Group Correlation Table

  • Presents correlations between specific wavenumbers (cm⁻¹) and functional groups (e.g., O-H, C-H, N-H) in molecules.
  • Provides a guide to identifying functional groups in samples.
  • Ranges of wavenumbers are associated with different strengths of the correlation between functional groups and spectrum peaks.

FTIR Spectroscopy Further Details

  • Chemical compounds' atoms constantly move and vibrate.
  • Each vibration happens at a specific frequency related to the chemical bond and structure.
  • Matching IR light frequency with vibrational frequency causes absorption, exciting molecular vibrations.
  • Plotted data after IR light passes through the sample creates an IR spectrum.

ATR FTIR Spectroscopy (Attenuated Total Reflection)

  • A technique to analyze samples without prior preparation, especially solid and liquid ones.
  • Mechanism: IR beam interacts with the sample through an evanescent wave.
  • Evanescent Wave: Light traveling through a crystal (e.g., ATR crystal), some light slightly penetrates the sample.
  • Depth: Attenuated total reflection penetrates only a few microns of the sample (0.5-2 µm).
  • Advantages: Direct examination of samples in their solid or liquid state without further preparation is possible.

Chemical Structure of Agarose and Collagen

  • Shows the molecular structures of agarose and collagen.
  • Provides diagrams of the chemical composition and arrangement of the molecules. Agarose has a chain of sugar molecules, while collagen has a repeating amino acid structure.

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This quiz explores the principles and applications of FTIR spectroscopy in the characterization of hydrogels. It focuses on the interaction of infrared light with materials to analyze molecular structures and chemical bonding. Test your understanding of how this technique is employed in biomaterials research.

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