Biomaterials ENGR-UH 4810 Lab 3: Spectroscopy
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Questions and Answers

What technique does the lab primarily utilize for the characterization of hydrogels?

  • Mass Spectrometry
  • Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (correct)
  • Nuclear Magnetic Resonance Spectroscopy
  • Ultraviolet-Visible Spectroscopy

What does the IR spectrum represent in the context of chemical compounds?

  • The molecular weight of the substance
  • The frequencies of light that were absorbed by the sample (correct)
  • The color of the chemical species
  • The total mass of the compound

Which characteristic of chemical bonds does IR spectroscopy exploit?

  • Molecular polarity
  • Electronegativity levels
  • Vibrational frequencies of atoms (correct)
  • Bond lengths

Why is it important to analyze functional groups in polymeric biomaterials?

<p>To assess biological response, biocompatibility, and biodegradability (B)</p> Signup and view all the answers

How can drug-polymer interactions be studied using FTIR spectroscopy?

<p>By analyzing the disappearance or appearance of FTIR peaks (B)</p> Signup and view all the answers

What happens to the IR light when it interacts with water in a spectroscopy experiment?

<p>Specific frequencies are absorbed, creating a missing frequency pattern (B)</p> Signup and view all the answers

What is a major application of FTIR analysis in biomaterials?

<p>Identifying and quantifying chemical species through their functional groups (C)</p> Signup and view all the answers

What does FTIR stand for in the context of spectroscopy?

<p>Fourier Transform Infrared Spectroscopy (B)</p> Signup and view all the answers

What is the wavenumber range associated with amide I in the FTIR spectrum of collagen?

<p>1600-1700 cm-1 (C)</p> Signup and view all the answers

Which peak in the FTIR spectrum corresponds to the glycosidic linkages in agarose?

<p>~1000-1150 cm-1 (B)</p> Signup and view all the answers

What is the purpose of pipetting a drop of water on the crystal during FTIR testing?

<p>To serve as a background for the measurement (A)</p> Signup and view all the answers

What method is used to prepare agarose hydrogel for FTIR testing?

<p>Heat agarose powder with water (B)</p> Signup and view all the answers

Which amide band is associated with the N−H deformation in the FTIR spectrum of collagen?

<p>Amide II (A)</p> Signup and view all the answers

What is the purpose of the OPUS software in the FTIR testing procedure?

<p>To operate the FTIR instrument (D)</p> Signup and view all the answers

During FTIR testing of hydrogels, what should be included in the results section of the experiment?

<p>FTIR spectrum of agarose and collagen hydrogels (B)</p> Signup and view all the answers

What is the characteristic peak range for C−H stretching vibrations in the FTIR spectrum?

<p>~2800-3000 cm-1 (D)</p> Signup and view all the answers

What method should be used to reverse the order of the values on the x-axis in Excel?

<p>Right-click on the axis and select Format Axis (C)</p> Signup and view all the answers

How are collagen and agarose primarily classified in terms of their composition?

<p>Collagen is a protein and agarose is a polysaccharide (C)</p> Signup and view all the answers

What is the primary focus of the analysis conducted with the FTIR spectra for collagen and agarose?

<p>To identify the absorption peaks of the biopolymers (B)</p> Signup and view all the answers

What is one common source of error that can affect the accuracy of recorded data values in experiments?

<p>Improper temperature control during the experiment (A)</p> Signup and view all the answers

At what wavenumbers are characteristic absorption peaks expected for collagen and agarose hydrogels?

<p>Between 3000 cm-1 and 3500 cm-1 (D)</p> Signup and view all the answers

What is essential to include on the cover page of an individual lab report?

<p>Name, net ID, lab number, and title (B)</p> Signup and view all the answers

Which specific option needs to be checked under Axis Options when formatting the axis in Excel?

<p>Values in reverse order (A)</p> Signup and view all the answers

Which of the following is a correct reference format for citing a research article?

<p>Author(s): Title, Journal Name, Volume(Issue), pages. (D)</p> Signup and view all the answers

Flashcards

Infrared Spectroscopy

A chemical analysis technique that uses the interaction between infrared light and matter to identify and quantify chemical species.

IR Vibrational Frequency

Each unique chemical bond and compound has a specific vibrational frequency that absorbs light.

FTIR Spectroscopy

A widely used spectroscopy technique for characterizing biomaterials, specifically determining functional groups and molecular structure.

Functional Groups

Specific groups of atoms within a molecule that influence its properties and interactions.

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Attenuated Total Reflectance (ATR)

A technique for obtaining infrared spectra of samples, typically analyzing the reflection of infrared light.

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Drug-Polymer Interactions

Studies exploring how drugs interact with polymers, including changes in their FTIR spectra.

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Biomaterials

Materials used in biological applications or interacting with biological systems.

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Biocompatibility

The ability of a material to interact safely and be compatible within a biological system.

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Amide I Band

A peak in the FTIR spectrum around 1600-1700 cm-1, representing the stretching vibration of the C=O bond in the peptide backbone of collagen.

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Amide II Band

A peak in the FTIR spectrum around 1500-1550 cm-1, representing the bending vibration of the N-H bond and C-N stretching in the peptide backbone of collagen.

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Amide III Band

A peak in the FTIR spectrum around 1200-1300 cm-1, representing a complex combination of vibrations involving C-N stretching and N-H bending in the peptide backbone of collagen.

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Glycosidic Linkages

Covalent bonds that connect sugar molecules in polysaccharides, indicated in the FTIR spectrum by peaks around 1000-1150 cm-1 due to C-O-C stretching vibrations.

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C-H Stretching

Peaks in the FTIR spectrum around 2800-3000 cm-1, representing the vibration of C-H bonds, found in both collagen and agarose.

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Agarose Hydrogel

A type of hydrogel composed of polysaccharide chains, producing characteristic peaks in FTIR spectrum due to glycosidic linkages and C-H stretching.

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Collagen Hydrogel

A type of hydrogel made from collagen, a fibrous protein found in connective tissues, showing characteristic peaks in FTIR spectrum due to amide bands.

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FTIR Spectra of Collagen

The FTIR spectrum of collagen exhibits characteristic peaks due to the presence of amide bonds (C=O and N-H) and other functional groups like C-H and O-H. These peaks are located in specific regions of the wavenumber spectrum and are useful for identifying collagen.

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FTIR Spectra of Agarose

Agarose is a polysaccharide, so its FTIR spectrum primarily shows peaks related to the sugar backbone (C-O, C-H, O-H) and any associated functional groups. It is characterized by strong peaks in the fingerprint region (below 1500 cm-1).

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FTIR Spectra Difference (Collagen vs Agarose)

The FTIR spectra of collagen and agarose exhibit distinct differences mainly because of the different chemical structures of proteins and polysaccharides. Collagen shows strong amide peaks, while agarose has prominent peaks associated with sugar groups.

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Characteristic Peaks for Collagen

Collagen shows characteristic absorption peaks in the amide regions (around 1650 cm-1 for amide I and 1550 cm-1 for amide II). These peaks are crucial for identifying collagen in biomaterials.

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Characteristic Peaks for Agarose

Agarose exhibits distinct peaks in the fingerprint region of the FTIR spectrum (below 1500 cm-1). This region provides significant information about the structure of the polysaccharide.

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Error Sources in FTIR

Factors like sample preparation, instrument calibration, and environmental conditions can influence the accuracy of FTIR data. Improper sample handling, moisture, and even the type of FTIR instrument used can introduce errors.

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Spectroscopy

A general set of analytical techniques used to investigate the interaction of matter with electromagnetic radiation, providing information about molecular structure and composition.

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Study Notes

Biomaterials - ENGR-UH 4810, Fall 2024, Lab 3: Spectroscopy-based Characterization of Hydrogels

  • Course: Biomaterials, ENGR-UH 4810, Fall 2024
  • Lab: Spectroscopy-based Characterization of Hydrogels (Lab 3)
  • Instructor: Jeremy Teo, Shafiya Sabah
  • Objective: To characterize hydrogels using Attenuated Total Reflectance Fourier Transform Infrared (ATR FTIR) Spectroscopy
  • Infrared (IR) Spectroscopy: A chemical analysis technique using infrared light interactions with matter to determine chemical compounds and their bond vibrations. Different vibrations occur at unique frequencies.
  • IR Light Absorption: Chemical compounds absorb specific IR frequencies, exciting molecular vibrations. The resulting spectrum (plot of absorbed frequencies) is unique to each compound. This unique spectrum acts as a "chemical fingerprint."
  • FTIR Spectroscopy in Biomaterials: Widely used for characterizing biomaterials by identifying functional groups, which reveal structural information like biocompatibility, biodegradability, and potential drug-polymer interactions.
  • FTIR analysis of Polymer Biomaterials: Predicts the molecular structure by analyzing absorbed/transmitted light, revealing functional groups.
  • Collagen FTIR Spectrum: Displays typical amide bands (peaks at ~1600-1700 cm⁻¹ (amide I), ~1500-1550 cm⁻¹ (amide II), and ~1200-1300 cm⁻¹ (amide III)). These peaks relate to C=O stretches and N-H deformation in collagen's peptide bonds.
  • Agarose Hydrogel FTIR Spectrum: Displays peaks relating to polysaccharide stretching vibrations (~2800-3000 cm⁻¹) and C-O-C stretching vibrations (~1000-1150 cm⁻¹) which indicate glycosidic linkages.
  • Materials: Agarose hydrogel, Collagen hydrogel
  • Methods: Preparing hydrogels in tubes. Agarose: heating agarose powder with water. Collagen: mixing collagen solution with buffer and incubating at 37°C.
  • FTIR System Setup: Testing the FTIR system using water as a background scan. Then, samples are placed on the crystal for scanning.
  • Results and Discussion: Includes FTIR spectra of agarose and collagen hydrogels, identifying and labeling characteristic peaks based on literature, contrasting the two, determining specific absorption locations, matching results with expected ranges and identifying potential error sources.

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Biomaterials Lab 3 Manual Fall 2024 PDF

Description

This quiz focuses on Lab 3 of the Biomaterials course, ENGR-UH 4810, where students will learn about spectroscopy-based characterization of hydrogels using ATR FTIR techniques. The objective is to understand how infrared light interacts with materials to identify chemical compounds through their unique spectral fingerprints.

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