Podcast
Questions and Answers
What technique does the lab primarily utilize for the characterization of hydrogels?
What technique does the lab primarily utilize for the characterization of hydrogels?
- Mass Spectrometry
- Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (correct)
- Nuclear Magnetic Resonance Spectroscopy
- Ultraviolet-Visible Spectroscopy
What does the IR spectrum represent in the context of chemical compounds?
What does the IR spectrum represent in the context of chemical compounds?
- The molecular weight of the substance
- The frequencies of light that were absorbed by the sample (correct)
- The color of the chemical species
- The total mass of the compound
Which characteristic of chemical bonds does IR spectroscopy exploit?
Which characteristic of chemical bonds does IR spectroscopy exploit?
- Molecular polarity
- Electronegativity levels
- Vibrational frequencies of atoms (correct)
- Bond lengths
Why is it important to analyze functional groups in polymeric biomaterials?
Why is it important to analyze functional groups in polymeric biomaterials?
How can drug-polymer interactions be studied using FTIR spectroscopy?
How can drug-polymer interactions be studied using FTIR spectroscopy?
What happens to the IR light when it interacts with water in a spectroscopy experiment?
What happens to the IR light when it interacts with water in a spectroscopy experiment?
What is a major application of FTIR analysis in biomaterials?
What is a major application of FTIR analysis in biomaterials?
What does FTIR stand for in the context of spectroscopy?
What does FTIR stand for in the context of spectroscopy?
What is the wavenumber range associated with amide I in the FTIR spectrum of collagen?
What is the wavenumber range associated with amide I in the FTIR spectrum of collagen?
Which peak in the FTIR spectrum corresponds to the glycosidic linkages in agarose?
Which peak in the FTIR spectrum corresponds to the glycosidic linkages in agarose?
What is the purpose of pipetting a drop of water on the crystal during FTIR testing?
What is the purpose of pipetting a drop of water on the crystal during FTIR testing?
What method is used to prepare agarose hydrogel for FTIR testing?
What method is used to prepare agarose hydrogel for FTIR testing?
Which amide band is associated with the N−H deformation in the FTIR spectrum of collagen?
Which amide band is associated with the N−H deformation in the FTIR spectrum of collagen?
What is the purpose of the OPUS software in the FTIR testing procedure?
What is the purpose of the OPUS software in the FTIR testing procedure?
During FTIR testing of hydrogels, what should be included in the results section of the experiment?
During FTIR testing of hydrogels, what should be included in the results section of the experiment?
What is the characteristic peak range for C−H stretching vibrations in the FTIR spectrum?
What is the characteristic peak range for C−H stretching vibrations in the FTIR spectrum?
What method should be used to reverse the order of the values on the x-axis in Excel?
What method should be used to reverse the order of the values on the x-axis in Excel?
How are collagen and agarose primarily classified in terms of their composition?
How are collagen and agarose primarily classified in terms of their composition?
What is the primary focus of the analysis conducted with the FTIR spectra for collagen and agarose?
What is the primary focus of the analysis conducted with the FTIR spectra for collagen and agarose?
What is one common source of error that can affect the accuracy of recorded data values in experiments?
What is one common source of error that can affect the accuracy of recorded data values in experiments?
At what wavenumbers are characteristic absorption peaks expected for collagen and agarose hydrogels?
At what wavenumbers are characteristic absorption peaks expected for collagen and agarose hydrogels?
What is essential to include on the cover page of an individual lab report?
What is essential to include on the cover page of an individual lab report?
Which specific option needs to be checked under Axis Options when formatting the axis in Excel?
Which specific option needs to be checked under Axis Options when formatting the axis in Excel?
Which of the following is a correct reference format for citing a research article?
Which of the following is a correct reference format for citing a research article?
Flashcards
Infrared Spectroscopy
Infrared Spectroscopy
A chemical analysis technique that uses the interaction between infrared light and matter to identify and quantify chemical species.
IR Vibrational Frequency
IR Vibrational Frequency
Each unique chemical bond and compound has a specific vibrational frequency that absorbs light.
FTIR Spectroscopy
FTIR Spectroscopy
A widely used spectroscopy technique for characterizing biomaterials, specifically determining functional groups and molecular structure.
Functional Groups
Functional Groups
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Attenuated Total Reflectance (ATR)
Attenuated Total Reflectance (ATR)
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Drug-Polymer Interactions
Drug-Polymer Interactions
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Biomaterials
Biomaterials
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Biocompatibility
Biocompatibility
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Amide I Band
Amide I Band
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Amide II Band
Amide II Band
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Amide III Band
Amide III Band
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Glycosidic Linkages
Glycosidic Linkages
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C-H Stretching
C-H Stretching
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Agarose Hydrogel
Agarose Hydrogel
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Collagen Hydrogel
Collagen Hydrogel
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FTIR Spectra of Collagen
FTIR Spectra of Collagen
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FTIR Spectra of Agarose
FTIR Spectra of Agarose
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FTIR Spectra Difference (Collagen vs Agarose)
FTIR Spectra Difference (Collagen vs Agarose)
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Characteristic Peaks for Collagen
Characteristic Peaks for Collagen
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Characteristic Peaks for Agarose
Characteristic Peaks for Agarose
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Error Sources in FTIR
Error Sources in FTIR
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Spectroscopy
Spectroscopy
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Study Notes
Biomaterials - ENGR-UH 4810, Fall 2024, Lab 3: Spectroscopy-based Characterization of Hydrogels
- Course: Biomaterials, ENGR-UH 4810, Fall 2024
- Lab: Spectroscopy-based Characterization of Hydrogels (Lab 3)
- Instructor: Jeremy Teo, Shafiya Sabah
- Objective: To characterize hydrogels using Attenuated Total Reflectance Fourier Transform Infrared (ATR FTIR) Spectroscopy
- Infrared (IR) Spectroscopy: A chemical analysis technique using infrared light interactions with matter to determine chemical compounds and their bond vibrations. Different vibrations occur at unique frequencies.
- IR Light Absorption: Chemical compounds absorb specific IR frequencies, exciting molecular vibrations. The resulting spectrum (plot of absorbed frequencies) is unique to each compound. This unique spectrum acts as a "chemical fingerprint."
- FTIR Spectroscopy in Biomaterials: Widely used for characterizing biomaterials by identifying functional groups, which reveal structural information like biocompatibility, biodegradability, and potential drug-polymer interactions.
- FTIR analysis of Polymer Biomaterials: Predicts the molecular structure by analyzing absorbed/transmitted light, revealing functional groups.
- Collagen FTIR Spectrum: Displays typical amide bands (peaks at ~1600-1700 cm⁻¹ (amide I), ~1500-1550 cm⁻¹ (amide II), and ~1200-1300 cm⁻¹ (amide III)). These peaks relate to C=O stretches and N-H deformation in collagen's peptide bonds.
- Agarose Hydrogel FTIR Spectrum: Displays peaks relating to polysaccharide stretching vibrations (~2800-3000 cm⁻¹) and C-O-C stretching vibrations (~1000-1150 cm⁻¹) which indicate glycosidic linkages.
- Materials: Agarose hydrogel, Collagen hydrogel
- Methods: Preparing hydrogels in tubes. Agarose: heating agarose powder with water. Collagen: mixing collagen solution with buffer and incubating at 37°C.
- FTIR System Setup: Testing the FTIR system using water as a background scan. Then, samples are placed on the crystal for scanning.
- Results and Discussion: Includes FTIR spectra of agarose and collagen hydrogels, identifying and labeling characteristic peaks based on literature, contrasting the two, determining specific absorption locations, matching results with expected ranges and identifying potential error sources.
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Description
This quiz focuses on Lab 3 of the Biomaterials course, ENGR-UH 4810, where students will learn about spectroscopy-based characterization of hydrogels using ATR FTIR techniques. The objective is to understand how infrared light interacts with materials to identify chemical compounds through their unique spectral fingerprints.