Biology Unit 2: Molecules and Membranes

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Questions and Answers

What type of bond forms between the 5'-phosphate of one nucleotide and the 3'-OH of another nucleotide?

  • Hydrogen bond
  • Phosphodiester bond (correct)
  • Ionic bond
  • Covalent bond

Which of the following is NOT a function of RNA?

  • Stores genetic information (correct)
  • Modulates gene expression
  • Involved in protein synthesis
  • Carries information from DNA

Which nitrogenous base is found in RNA but not in DNA?

  • Thymine
  • Adenine
  • Uracil (correct)
  • Cytosine

In what direction are polynucleotides synthesized?

<p>5' to 3' (D)</p> Signup and view all the answers

What structural feature distinguishes DNA from RNA?

<p>Containing thymine instead of uracil (D)</p> Signup and view all the answers

What is the role of cholesterol-derived hormones like testosterone and estradiol?

<p>Regulate intercellular signaling (D)</p> Signup and view all the answers

Which component contributes to the amphipathic nature of cholesterol?

<p>Hydroxyl group (D)</p> Signup and view all the answers

What is the primary information molecule in cells?

<p>DNA (B)</p> Signup and view all the answers

What is the main function of polysaccharides mentioned?

<p>Markers for cell recognition processes (A)</p> Signup and view all the answers

Which formula represents monosaccharides?

<p>(CH2O)n (B)</p> Signup and view all the answers

Which statement is true about cyclized sugars?

<p>They are predominant forms within cells. (D)</p> Signup and view all the answers

What type of bond joins monosaccharides together?

<p>Glycosidic bond (C)</p> Signup and view all the answers

How does cellulose differ from starch?

<p>Cellulose is a structural component, while starch is for storage. (C)</p> Signup and view all the answers

Which of the following polysaccharides is formed mainly of glucose?

<p>Cellulose (B)</p> Signup and view all the answers

What type of bonds primarily link glucose molecules in glycogen and starch?

<p>a(1 → 4) and a(1 → 6) bonds (D)</p> Signup and view all the answers

What role do carbohydrates play in cell signaling?

<p>They function as markers to direct proteins. (B)</p> Signup and view all the answers

What percentage of the human body weight is made up by oxygen, hydrogen, carbon, and nitrogen?

<p>96.2% (D)</p> Signup and view all the answers

Which of the following is true about water in cells?

<p>It represents 70% or more of cell mass. (B)</p> Signup and view all the answers

What is the primary function of carbohydrates in cells?

<p>Providing energy and serving as starting materials for other compounds. (A)</p> Signup and view all the answers

Which of the following statements about inorganic ions in cells is correct?

<p>They are involved in aspects of cellular metabolism. (C)</p> Signup and view all the answers

Which organic molecule type makes up over 90% of the dry weight of most cells?

<p>Nucleic acids (A), Lipids (B), Proteins (C), Carbohydrates (D)</p> Signup and view all the answers

How do nonpolar molecules behave in an aqueous medium?

<p>They tend to minimize contact with water. (C)</p> Signup and view all the answers

Which of the following ions is categorized as a monovalent cation?

<p>Sodium (Na+) (D)</p> Signup and view all the answers

What role do polysaccharides play in cells?

<p>They store energy and provide structural support. (A)</p> Signup and view all the answers

What is the primary role of phospholipids in cells?

<p>They are the main components of cell membranes. (C)</p> Signup and view all the answers

What characterizes the structure of fatty acids?

<p>They contain a carboxyl group and a hydrocarbon chain. (A)</p> Signup and view all the answers

How are triglycerides formed?

<p>By combining one glycerol molecule with three fatty acids. (A)</p> Signup and view all the answers

Why are triglycerides more efficient energy storage than carbohydrates?

<p>They provide more than twice the energy per weight of degraded material. (A)</p> Signup and view all the answers

What is a key feature of phospholipids that allows them to form membranes?

<p>They are amphipathic with both hydrophobic and hydrophilic parts. (A)</p> Signup and view all the answers

What type of molecule is sphingomyelin?

<p>A non-glyceric phospholipid in cell membranes. (A)</p> Signup and view all the answers

What is the primary role of lipid signaling molecules such as steroid hormones?

<p>To act as molecular messengers carrying signals within cells. (D)</p> Signup and view all the answers

What do glycolipids contribute to cell membranes?

<p>They enhance membrane fluidity and play roles in cell signaling. (A)</p> Signup and view all the answers

What is the primary function of ATP within cells?

<p>Main form of chemical energy (A)</p> Signup and view all the answers

What distinguishes polar amino acids from nonpolar amino acids?

<p>Polar amino acids are hydrophilic and tend to be on the outside of proteins (D)</p> Signup and view all the answers

Which statement about the peptide bonds between amino acids is accurate?

<p>They link the alpha-carboxyl group of one amino acid to the alpha-amino group of another (D)</p> Signup and view all the answers

What is the significance of the N-terminal and C-terminal ends of a polypeptide?

<p>They denote the direction of protein synthesis (A)</p> Signup and view all the answers

Why are proteins considered the most varied macromolecules?

<p>They perform a wide range of functions across different processes (C)</p> Signup and view all the answers

What role do proteins play in the immune response?

<p>They provide defenses against infection (A)</p> Signup and view all the answers

How did Frederick Sanger contribute to the understanding of proteins in 1953?

<p>He was the first to determine the complete sequence of a protein (B)</p> Signup and view all the answers

What aspect of proteins is primarily responsible for their function?

<p>Their characteristic three-dimensional configurations (A)</p> Signup and view all the answers

What defines the primary structure of proteins?

<p>The sequence of amino acids in a polypeptide chain (D)</p> Signup and view all the answers

Which of the following describes the secondary structure of proteins?

<p>The structured arrangement of localized regions of amino acids (B)</p> Signup and view all the answers

What crucial role do hydrogen bonds play in protein structures?

<p>They maintain secondary structures. (A)</p> Signup and view all the answers

How do phospholipids contribute to the structure of cell membranes?

<p>They form a double layer due to their fatty acid tails. (C)</p> Signup and view all the answers

What is the effect of temperature on the fluidity of lipid bilayers?

<p>Fluidity increases with higher temperatures. (B)</p> Signup and view all the answers

Which structure is responsible for defining the internal compartments of eukaryotic cells?

<p>Cell membranes (A)</p> Signup and view all the answers

What is one of the primary functions of membrane proteins?

<p>They act as electron transporters and receptors. (C)</p> Signup and view all the answers

Which characteristic of lipid bilayers is crucial for their function?

<p>Their ability to behave like fluids. (A)</p> Signup and view all the answers

Flashcards

What are cells?

The primary building blocks of cells, capable of replicating and performing specialized tasks in multicellular organisms.

What are the three fundamental elements of cells?

Water, inorganic ions, and organic molecules (containing carbon).

Describe the properties of water.

A molecule with a slightly positive charge on the hydrogen atoms and a slightly negative charge on the oxygen atom, allowing it to form hydrogen bonds.

What is the significance of inorganic ions in cells?

Inorganic ions represent less than 1% of the cell's mass. They are essential for various metabolic processes and play a critical role in cellular functions.

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What are organic molecules?

Macromolecules that make up over 90% of the dry weight of cells. They consist of carbohydrates, lipids, proteins, and nucleic acids.

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What is the role of carbohydrates in cells?

Simple sugars (monosaccharides) are the primary energy source for cells. Their breakdown provides energy and starting material for the synthesis of other cellular compounds.

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What are polysaccharides and their functions?

Polysaccharides are complex carbohydrates made up of long chains of simple sugars. They serve as energy storage and structural components of the cell.

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What are proteins?

A long chain of repeating units of amino acids. Proteins have various functions including structural support, enzymatic activity, transport, defense, and communication.

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Monosaccharide

The basic building block of carbohydrates. It has the general formula (CH2O)n, where n is the number of carbon atoms. Glucose, the main source of energy in cells, is a common example.

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Cyclized Sugar

A cyclic form of a monosaccharide with 5 or more carbons. It exists in two forms: alpha (α) and beta (β), depending on the configuration of the first carbon atom (C1).

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Glycosidic Bond

A covalent bond that links two monosaccharides by removing a water molecule. It forms between two carbon atoms.

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Oligosaccharide

A short chain of monosaccharides linked by glycosidic bonds. It can have a few to several sugar units.

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Polysaccharide

A long chain of monosaccharides linked by glycosidic bonds. They can have hundreds or thousands of sugar units.

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Glycogen

The main storage form of carbohydrates in animals. It is composed of glucose units linked by α(1→4) glycosidic bonds. It has branches with α(1→6) bonds.

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Starch

The main storage form of carbohydrates in plants. It is composed of amylose and amylopectin. Amylose is a linear chain of glucose units linked by α(1→4) bonds. Amylopectin also has branches with α(1→6) bonds.

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Cellulose

The primary structural component of plant cell walls. It is composed of glucose units linked by β(1→4) glycosidic bonds. Its linear chains are packed together to form strong fibers.

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What are fatty acids?

Simple lipids with a long hydrocarbon chain and a carboxyl group at one end. They are hydrophobic due to their nonpolar C-H bonds which makes them insoluble in water.

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What are triglycerides?

Stored form of fatty acids, composed of three fatty acid molecules linked to a glycerol molecule. Primarily used as an energy reserve for cells, providing more than twice the energy per weight compared to carbohydrates.

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What are phospholipids?

Amphipathic molecules, containing both hydrophobic (nonpolar) tails and hydrophilic (polar) heads, crucial components of cell membranes.

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What are phosphoglycerides?

A type of phospholipid where two fatty acid chains are attached to a glycerol molecule. The third carbon of glycerol is linked to a phosphate group, which in turn can bind to various other polar molecules. They are essential for cell membrane structure and function.

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What is sphingomyelin?

A non-glyceric phospholipid found in cell membranes, where two fatty acid chains are linked to a polar head group containing serine instead of glycerol. It plays a crucial role in cell signaling and membrane stability.

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What is cholesterol?

Lipid molecules that are present in cell membranes alongside phospholipids. They are involved in maintaining membrane fluidity and regulate the permeability of cell membranes.

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What are glycolipids?

Lipids that have a sugar molecule attached to their structure. They are located primarily in the outer leaflet of cell membranes and play roles in cell recognition and communication.

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What is the overall importance of lipids in cells?

Lipids are essential molecules in cells. They serve as energy sources, building blocks for membranes, and play vital roles in cell signaling.

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What are nucleic acids?

Large organic molecules that carry genetic information. They are composed of nucleotides, which are made up of a sugar, a phosphate group, and a nitrogenous base.

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What is DNA?

A type of nucleic acid that carries genetic information in the form of a double helix. It's made up of two strands of nucleotides held together by hydrogen bonds between complementary bases.

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What is mRNA?

A type of nucleic acid that plays a crucial role in protein synthesis. It carries genetic information from DNA to ribosomes, where it serves as a template for protein production.

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How are nucleic acids formed?

The process by which nucleotides are joined together to form a nucleic acid chain. It involves the formation of a phosphodiester bond between the 5'-phosphate of one nucleotide and the 3'-OH of the next.

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What is the direction of nucleic acid synthesis?

The direction in which nucleotides are added during the synthesis of nucleic acids. New nucleotides are always added to the 3'-OH group of the growing chain.

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What is the role of the base sequence in nucleic acids?

The sequence of bases in a polynucleotide chain that determines the genetic code. It dictates the order of amino acids in a protein, ultimately influencing its structure and function.

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What is ATP?

The main energy currency used by cells, providing energy for various metabolic processes.

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What is cAMP's role in cells?

Cyclic adenosine monophosphate (cAMP) is a crucial second messenger in signaling pathways, relaying signals from the cell surface to intracellular targets.

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What are the main functions of proteins?

Proteins are the workhorses of the cell, responsible for diverse functions such as structural support, transport, defense against infections, enzymatic activity, and communication.

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What are the components of an amino acid?

Amino acids are the building blocks of proteins. Each amino acid consists of a central carbon atom linked to an amino group, a carboxyl group, a hydrogen atom, and a unique side chain (R group).

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How does the side chain (R group) affect an amino acid?

The side chain (R group) of an amino acid determines its chemical properties, dictating its interaction with other molecules and its role in protein structure and function.

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What is a peptide bond?

A peptide bond is a covalent bond that connects two amino acids. It is formed between the carboxyl group of one amino acid and the amino group of the next amino acid.

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What is a polypeptide chain?

A polypeptide chain is a linear sequence of amino acids linked by peptide bonds. Proteins are made up of one or more polypeptide chains.

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Why is protein folding important?

Proteins fold into intricate three-dimensional structures that are crucial for their function. These structures are determined by the sequence of amino acids and interactions between them.

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Primary Structure of Protein

The linear sequence of amino acids in a polypeptide chain, forming the building block for all protein structures.

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Secondary Structure of Protein

The local spatial arrangements of amino acids within a polypeptide chain, stabilized by hydrogen bonds. Most commonly observed structures include the α helix and the β sheet.

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Tertiary Structure of Protein

The three-dimensional folding of a single polypeptide chain, determined by interactions between amino acid side chains. These interactions include hydrogen bonds, ionic bonds, hydrophobic interactions, and disulfide bridges.

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Quaternary Structure of Protein

The arrangement of multiple polypeptide chains (subunits) within a protein, stabilized by interactions similar to those found in tertiary structure. These subunits can be identical or different.

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Phospholipid Bilayer

The basic structural unit of all biological membranes, composed of two layers of phospholipid molecules. The hydrophobic tails of the phospholipids point inwards, while the hydrophilic heads face outwards.

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Membrane Proteins

Proteins embedded within the phospholipid bilayer, performing various functions. Examples include transporters, receptors, and enzymes.

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Membrane Fluidity

The ability of membrane lipids and proteins to move laterally within the bilayer, contributing to its fluidity and functionality.

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Selective Permeability

The property of a membrane that allows certain molecules to pass through it, while blocking others. This selective permeability is crucial for maintaining cellular homeostasis.

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Study Notes

Unit 2: Molecules and Membranes

  • This unit introduces the fundamental molecules and membranes within cells.

Section 1: Introduction

  • Molecules and membranes are key components of cells.

Index

  • 2.1 Cell Molecules:
    • Carbohydrates (sugars)
    • Lipids (fats)
    • Nucleic acids (DNA, RNA)
    • Proteins
  • 2.2 Cell Membranes:
    • Membrane lipids (phospholipids, glycolipids, cholesterol)
    • Membrane proteins (integral and peripheral)
    • Transport across cell membranes (passive and active)

2.1 Cell Molecules

  • Cells are complex structures with specialized functions.
  • Cells are composed of water, inorganic ions, and organic molecules (containing carbon).

Water

  • Water accounts for a significant percentage (70% or more) of cell mass.
  • Water is a polar molecule with slightly positive hydrogen atoms and a slightly negative oxygen atom.
  • This polarity allows water to form hydrogen bonds with other polar molecules and charged ions.
  • Water molecules interact with polar molecules (hydrophilic), while nonpolar molecules (hydrophobic) do not interact well with water.

Inorganic Ions

  • Inorganic ions represent a smaller percentage (1% or less) of cell mass.
  • These ions play critical roles in cellular functions and metabolism.
  • Examples include sodium (Na+), potassium (K+), magnesium (Mg2+), calcium (Ca2+), chloride (Cl-), hydrogen phosphate (HPO42-), and bicarbonate (HCO3-).

Organic Molecules

  • Organic molecules make up over 90% of the dry weight of most cells.
  • The molecules are diverse, including carbohydrates, lipids, proteins, and nucleic acids.
  • Macromolecules are formed by polymerizing smaller precursors (monomers).

Carbohydrates

  • Simple sugars (monosaccharides): Main cellular nutrients with energy functions.
  • Polysaccharides: These are structural components and energy storage forms.
  • Examples include glycogen (animal cells) and starch (plant cells).
  • Cellulose provides structural support in plant cell walls.

Monosaccharides

  • The basic formula is (CH2O)n, where n is the number of carbons.
  • Glucose is a primary source of energy in cells.
  • Other monosaccharides have between 3-7 carbons.
  • Sugars with 5 or more carbons often form ring structures in cells.
  • Glucose exists in two forms, α and β, depending on the placement of the hydroxyl group on C1.

Glycosidic Bonds

  • Monosaccharides link together through dehydration reactions to form polysaccharides.
  • The resulting bonds are glycosidic bonds.
  • If only a few sugars are attached, the resulting polymer is called an oligosaccharide
  • Polymers of hundreds or thousands of sugars are called polysaccharides.

Glycogen and Starch

  • Glycogen is the storage form of carbohydrates in animals.
  • Starch stores carbohydrates in plants
  • Both glycogen and starch are composed of glucose molecules.
  • Glycogen is more highly branched than starch.

Cellulose

  • Cellulose is a structural component of plant cells (cell walls).
  • It consists of glucose molecules linked by β (1→4) glycosidic bonds.
  • Straight chains of cellulose molecules are packed together to form strong fibers that provide structural integrity.

Lipids

  • Lipids are mainly composed of hydrocarbon chains with a carboxyl group.
  • Fatty acids (the simplest lipids) contain long hydrocarbon chains and a carboxyl group.
  • Triglycerides are energy storage molecules formed from glycerol and fatty acids.
  • Cell membranes are composed primarily of phospholipids (composed of fatty acids, glycerol, and a phosphate group).

Phospholipids

  • Phospholipids are amphipathic molecules: both hydrophobic and hydrophilic, forming cell membranes.
  • Hydrophobic tails of the phospholipids orient inward, and the hydrophilic heads face outward.
  • Phospholipids form a bilayer, with the hydrophobic tails facing each other and the hydrophilic heads contacting the aqueous environment.
  • The phospholipid bilayer is responsible for compartmentalization within cells.

Cholesterol

  • Cholesterol is a component of animal cell membranes.
  • It has a steroid structure differing from phospholipids in that it contains a fused ring structure.
  • Cholesterol affects the fluidity of the membrane, preventing excessive rigidity at low temperatures and excessive fluidity at high temperatures

Nucleic Acids

  • Nucleic acids are the primary information carriers of cells.
  • DNA (deoxyribonucleic acid) is the blueprint of genetic material in most organisms.
  • RNA (ribonucleic acid) is involved in protein synthesis.
  • RNA comes in different types (mRNA, rRNA, tRNA), each with specific functions.

Nucleotides

  • Nucleotides are the building blocks of nucleic acids.
  • Nucleotides consist of a nitrogenous base, a sugar (deoxyribose or ribose), and a phosphate group.
  • Purines (adenine and guanine) and pyrimidines (cytosine, thymine, and uracil) are examples of nitrogenous bases

Proteins

  • Proteins are a diverse group of macromolecules performing essential tasks in the cell.
  • Proteins are polymers of amino acids connected by peptide bonds.
  • Amino acids have 20 different types; their properties (polar, nonpolar, acidic, basic) affect protein structure and function.

Protein Structure

  • Primary: The amino acid sequence of a polypeptide chain.
  • Secondary: Hydrogen bond formation between amino acid backbones (alpha helix, beta sheet).
  • Tertiary: The three-dimensional folding of the polypeptide.
  • Quaternary: Multiple polypeptide chains interacting to form a functional protein complex.

Cell Membranes

  • Cell membranes separate the inside of the cell from its surroundings.
  • All cell membranes are composed of a phospholipid bilayer and proteins embedded within it.
  • The phospholipid bilayer regulates the passage of substances across the membrane.

Membrane Proteins

  • Integral proteins are embedded in the lipid bilayer, often spanning the entire width of the membrane forming channels or carriers that help move substances across the membrane.
  • Peripheral proteins are associated with the membrane surface (inside or outside)

Transport Across Membranes

  • Passive transport involves the movement of molecules from an area of high concentration to an area of low concentration (along a concentration gradient).
  • Active transport involves the movement of molecules against a concentration gradient using energy (ATP).
  • Membrane proteins (channels and carriers) assist in transport across membranes.

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