Biology: Understanding Living Organisms and Cells
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Questions and Answers

What does the term 'logy' in biology refer to?

  • Science of living organisms
  • Knowledge of cells
  • Theory of organisms
  • Study of life (correct)

Which of the following statements is part of the Cell Theory?

  • Living organisms are made up of one type of cell only.
  • Cells arise spontaneously from non-living matter.
  • All cells possess a nucleus.
  • Cells are the basic units of structure and function in all organisms. (correct)

What distinguishes prokaryotic cells from eukaryotic cells?

  • Prokaryotic cells have a nucleus.
  • Eukaryotic cells lack a plasma membrane.
  • Prokaryotic cells have a rigid cell wall. (correct)
  • Eukaryotic cells lack membrane-bound organelles.

Who was the first to observe microorganisms, significantly advancing the field of biology?

<p>Antoine van Leeuwenhoek (D)</p> Signup and view all the answers

What role do plasmids play in prokaryotic cells?

<p>They carry extra genes. (B)</p> Signup and view all the answers

Which of the following is true regarding the unique shape of a cell?

<p>Cell shape is influenced by its function. (C)</p> Signup and view all the answers

What is one characteristic of eukaryotic cells compared to prokaryotic cells?

<p>Eukaryotic cells have a complex nucleus. (A)</p> Signup and view all the answers

Which statement accurately describes eukaryotic cell organelles?

<p>Eukaryotic cells contain membranous organelles for various functions. (C)</p> Signup and view all the answers

What distinguishes rough endoplasmic reticulum from smooth endoplasmic reticulum?

<p>Rough ER contains ribosomes while smooth ER does not. (B)</p> Signup and view all the answers

Which of the following organelles are directly involved in cellular respiration?

<p>Mitochondria (A)</p> Signup and view all the answers

What is the primary function of the nucleolus within the nucleus?

<p>Assembling ribosomes (D)</p> Signup and view all the answers

How does the plasma membrane maintain its integrity and fluidity?

<p>Presence of cholesterol (D)</p> Signup and view all the answers

Which structure is responsible for anchoring cells in animal tissues?

<p>Desmosomes (B)</p> Signup and view all the answers

What role do ribosomes play in eukaryotic cells?

<p>Protein synthesis (A)</p> Signup and view all the answers

Which cellular structure is NOT part of the endomembrane system?

<p>Mitochondria (D)</p> Signup and view all the answers

Which cytoskeletal component is primarily involved in muscle contraction?

<p>Microfilaments (B)</p> Signup and view all the answers

What is the primary function of the Golgi apparatus?

<p>Modification and packaging of proteins (C)</p> Signup and view all the answers

What is the main role of chloroplasts in plant cells?

<p>Photosynthesis (C)</p> Signup and view all the answers

In eukaryotic cells, what does the term 'fluid mosaic model' refer to?

<p>Composition and structure of the plasma membrane (A)</p> Signup and view all the answers

What substance moves against concentration gradients during active transport?

<p>Ions (B)</p> Signup and view all the answers

What is the characteristic structure of eukaryotic ribosomes?

<p>Two subunits: large and small (D)</p> Signup and view all the answers

What is the function of the nuclear pore complex?

<p>Regulation of transport between the nucleus and cytoplasm (C)</p> Signup and view all the answers

What is the main purpose of meiosis in sexual reproduction?

<p>To create genetically diverse gametes (A)</p> Signup and view all the answers

What happens during the crossing-over event in meiosis?

<p>Non-sister chromatids exchange segments of DNA (D)</p> Signup and view all the answers

Which phase of meiosis is characterized by the separation of homologous chromosomes?

<p>Anaphase I (B)</p> Signup and view all the answers

What is a key difference between meiosis and mitosis?

<p>Meiosis produces four genetically distinct haploid cells (A)</p> Signup and view all the answers

What role does independent assortment play during meiosis?

<p>It increases genetic variation in gametes (D)</p> Signup and view all the answers

In which phase does the synapsis of homologous chromosomes occur?

<p>Prophase I (C)</p> Signup and view all the answers

What is the end result of meiosis II?

<p>Production of four haploid daughter cells (B)</p> Signup and view all the answers

What distinguishes the tetrad formation during meiosis?

<p>It involves homologous chromosomes pairing together (A)</p> Signup and view all the answers

What is the significance of chiasmata in meiosis?

<p>They are contact points for genetic material exchange (A)</p> Signup and view all the answers

Which process does NOT occur during meiosis?

<p>Replication of chromosomes before meiosis II (B)</p> Signup and view all the answers

How does meiosis contribute to genetic diversity?

<p>Through independent assortment and crossing-over (A)</p> Signup and view all the answers

During which stage are sister chromatids separated in meiosis?

<p>Anaphase II (C)</p> Signup and view all the answers

What is the reduction division in meiosis?

<p>The reduction of chromosome number from diploid to haploid (D)</p> Signup and view all the answers

What advantage does aerobic respiration have over anaerobic processes?

<p>It allows for more efficient energy production. (D)</p> Signup and view all the answers

What was a significant consequence of increased atmospheric oxygen levels?

<p>Development of the ozone layer (C)</p> Signup and view all the answers

Which process is central to the endosymbiotic theory?

<p>Engulfment of prokaryotic cells by eukaryotes (A)</p> Signup and view all the answers

What evidence supports the endosymbiotic theory regarding mitochondria and chloroplasts?

<p>Their double membranes and circular DNA (A)</p> Signup and view all the answers

What does Gibbs free energy (ΔG) indicate about a chemical reaction?

<p>The spontaneity of the reaction (D)</p> Signup and view all the answers

What role do enzymes play in biological reactions?

<p>They lower the activation energy required for reactions. (D)</p> Signup and view all the answers

How do coenzymes differ from cofactors?

<p>Cofactors assist enzyme functions and are inorganic. (D)</p> Signup and view all the answers

What is the primary energy carrier in cells?

<p>ATP (A)</p> Signup and view all the answers

What does the first law of thermodynamics state?

<p>Energy cannot be created or destroyed, only transformed. (B)</p> Signup and view all the answers

What is a key characteristic of exergonic reactions?

<p>They release energy. (D)</p> Signup and view all the answers

How does the Cambrian explosion relate to oxygen levels?

<p>Increased oxygen levels supported larger, more complex organisms. (B)</p> Signup and view all the answers

What is the significance of ATP hydrolysis in cellular processes?

<p>It releases energy for various cellular processes. (D)</p> Signup and view all the answers

What underlies the importance of chemical energy in biological processes?

<p>It drives metabolic reactions by releasing energy. (C)</p> Signup and view all the answers

What is the primary outcome of meiosis in terms of chromosome number?

<p>Chromosome number is reduced by half (A)</p> Signup and view all the answers

Which process introduces genetic variation during meiosis?

<p>Crossing Over (C)</p> Signup and view all the answers

What are the end products of meiosis?

<p>Four haploid cells (C)</p> Signup and view all the answers

What occurs during Metaphase I of meiosis?

<p>Bivalents align at the metaphase plate (C)</p> Signup and view all the answers

Which of the following describes a bivalent?

<p>A pair of homologous chromosomes (B)</p> Signup and view all the answers

What is the significance of crossing over?

<p>It increases genetic diversity (D)</p> Signup and view all the answers

During which phase does independent assortment occur?

<p>Metaphase I (C)</p> Signup and view all the answers

What is the role of meiosis in sexual reproduction?

<p>It reduces chromosome number and increases genetic diversity (A)</p> Signup and view all the answers

What are gametes?

<p>Reproductive cells that carry half the genetic information (C)</p> Signup and view all the answers

Which of the following best describes the outcome of Meiosis II?

<p>Four genetically distinct haploid cells (B)</p> Signup and view all the answers

The process of genetic variation through independent assortment is essential for which of the following?

<p>Reproduction and evolution of species (A)</p> Signup and view all the answers

Which phase follows Anaphase I in meiosis?

<p>Telophase I (C)</p> Signup and view all the answers

Why is genetic variation important for natural selection?

<p>It enables populations to adapt to changing environments (A)</p> Signup and view all the answers

What is the main function of the spindle apparatus during cell division?

<p>To separate sister chromatids (A)</p> Signup and view all the answers

Which phase of the cell cycle involves the duplication of DNA?

<p>S Phase (B)</p> Signup and view all the answers

What does the process of cytokinesis result in?

<p>Division of the cytoplasm (C)</p> Signup and view all the answers

In which phase do chromosomes align at the spindle midpoint?

<p>Metaphase (D)</p> Signup and view all the answers

Which of the following statements accurately describes the role of the kinetochore?

<p>It attaches chromosomes to microtubules during anaphase (D)</p> Signup and view all the answers

What type of cell cycle do prokaryotic cells undergo?

<p>Binary fission (B)</p> Signup and view all the answers

Which characteristic is NOT typically associated with living organisms?

<p>Independent metabolism (A)</p> Signup and view all the answers

What is a fundamental component of a virus?

<p>Protein coat (B)</p> Signup and view all the answers

According to the reducing atmosphere hypothesis, which condition facilitated the formation of organic molecules?

<p>Electron donation (D)</p> Signup and view all the answers

What results from oxygenic photosynthesis?

<p>Generation of energy from sunlight (A)</p> Signup and view all the answers

Which of the following best describes the Miller-Urey experiment?

<p>Synthesized amino acids from abiotic conditions (B)</p> Signup and view all the answers

What happens during the anaphase stage of mitosis?

<p>Sister chromatids separate and move to opposite poles (D)</p> Signup and view all the answers

What is the main role of checkpoints within the cell cycle?

<p>To ensure proper division and function (C)</p> Signup and view all the answers

Which of the following structures is involved in the formation of the cleavage furrow during cytokinesis in animal cells?

<p>Actin filaments (B)</p> Signup and view all the answers

Which type of cells utilize a cell plate during cytokinesis?

<p>Plant cells (B)</p> Signup and view all the answers

What is the role of common electron carriers such as NAD+/NADH and FAD/FADH2 in cellular processes?

<p>They facilitate electron transfer in metabolic pathways. (C)</p> Signup and view all the answers

In which cellular organelle does photosynthesis primarily occur?

<p>Chloroplasts (A)</p> Signup and view all the answers

What concept proposed by Hippocrates relates to the early understanding of genetics?

<p>Pangenesis (C)</p> Signup and view all the answers

Which of Mendel's laws states that alleles segregate during gamete formation?

<p>Law of Segregation (C)</p> Signup and view all the answers

What does codominance in genetics illustrate?

<p>Both parental traits are equally expressed in offspring. (D)</p> Signup and view all the answers

Who is considered the 'Father of Genetics' due to his experiments with pea plants?

<p>Gregor Mendel (D)</p> Signup and view all the answers

What is a Punnett square used for in genetics?

<p>To predict genotypic and phenotypic ratios of offspring. (B)</p> Signup and view all the answers

In genetics, what does the term 'pleiotropy' refer to?

<p>Multiple traits influenced by a single gene. (C)</p> Signup and view all the answers

What misconception was associated with the blending theory of inheritance?

<p>Offspring are a blend of traits from both parents. (D)</p> Signup and view all the answers

During meiosis, what accounts for Mendel's principles of inheritance patterns?

<p>The behavior of chromosomes. (C)</p> Signup and view all the answers

Which statement best describes dominant and recessive alleles?

<p>Dominant alleles mask the expression of recessive alleles. (A)</p> Signup and view all the answers

What theory did Jean-Baptiste Lamarck propose regarding inheritance?

<p>Traits acquired during an organism's life can be inherited. (C)</p> Signup and view all the answers

What significance does the behavior of chromosomes during gamete formation have?

<p>It leads to the separation of homologous chromosomes. (D)</p> Signup and view all the answers

What was one of the major contributions of Aristotle to early biological thought?

<p>Classification of organisms. (D)</p> Signup and view all the answers

What role do enzymes play in metabolic processes?

<p>They lower the activation energy, speeding up reactions. (A)</p> Signup and view all the answers

Which statement correctly describes the active site of an enzyme?

<p>It serves as the site where substrate binding and catalysis take place. (C)</p> Signup and view all the answers

How is energy coupling significant in metabolism?

<p>It utilizes energy from exergonic reactions to power endergonic processes. (D)</p> Signup and view all the answers

What is activation energy (EA) in the context of chemical reactions?

<p>It is the energy needed for reactants to reach the transition state. (B)</p> Signup and view all the answers

What distinguishes competitive inhibitors from non-competitive inhibitors?

<p>Competitive inhibitors compete for the active site against the substrate. (A)</p> Signup and view all the answers

What does Gibbs free energy (G) indicate about a reaction?

<p>It indicates the spontaneity and maximum energy available for work. (A)</p> Signup and view all the answers

Which of the following factors can influence enzyme activity?

<p>Temperature, pH, and substrate concentration. (D)</p> Signup and view all the answers

What is the primary function of coenzymes in enzyme-catalyzed reactions?

<p>They assist enzymes in catalyzing reactions. (D)</p> Signup and view all the answers

How does temperature generally affect enzyme activity?

<p>Optimal temperature ranges are crucial for maintaining enzyme efficiency. (D)</p> Signup and view all the answers

What type of reaction is cellular respiration considered in terms of Gibbs free energy?

<p>Exergonic, since it releases energy. (A)</p> Signup and view all the answers

Which of the following statements about enzyme specificity is correct?

<p>Enzymes are specific to their substrate, often explained by the lock-and-key model. (D)</p> Signup and view all the answers

What impacts the rate of a chemical reaction in metabolic pathways besides enzyme concentration?

<p>The temperature and pH of the environment. (B)</p> Signup and view all the answers

Which of the following best explains energy transfer involving hydrogen atoms in biological systems?

<p>They are crucial for the transfer of electrons, vital for energy production. (C)</p> Signup and view all the answers

What are enzyme inhibitors typically used for in practical applications?

<p>To target specific enzymes for therapeutic purposes. (B)</p> Signup and view all the answers

What is the significance of Mendel's law of segregation during gamete formation?

<p>It ensures that gametes each receive only one allele from each pair. (D)</p> Signup and view all the answers

In Mendel's dihybrid crosses, what is the typical phenotypic ratio observed?

<p>9:3:3:1 (D)</p> Signup and view all the answers

What phenomenon can lead to deviations from expected phenotypic ratios in dihybrid crosses?

<p>Linkage between traits (C)</p> Signup and view all the answers

Which type of genetic mapping can be created using recombination frequencies?

<p>Linkage maps (D)</p> Signup and view all the answers

How does crossing over during meiosis affect genetic variation?

<p>It introduces new allele combinations in the offspring. (C)</p> Signup and view all the answers

What directly transfers a phosphate group to ADP to form ATP in substrate-level phosphorylation?

<p>Phosphorylated intermediate (A)</p> Signup and view all the answers

What sex chromosome composition defines a male in humans?

<p>XY (C)</p> Signup and view all the answers

Which condition is an example of a disorder related to sex-linked inheritance?

<p>Turner syndrome (B)</p> Signup and view all the answers

Which type of phosphorylation is driven by light energy during photosynthesis?

<p>Photophosphorylation (D)</p> Signup and view all the answers

What are X-linked recessive disorders more prevalent in males?

<p>Males have one X chromosome. (D)</p> Signup and view all the answers

What type of reaction involves the transfer of electrons and plays a key role in ATP production?

<p>Redox reaction (B)</p> Signup and view all the answers

What is a key characteristic of mitochondrial DNA inheritance?

<p>It is inherited maternally. (C)</p> Signup and view all the answers

What is the final electron acceptor in oxidative phosphorylation?

<p>Oxygen (A)</p> Signup and view all the answers

Which electron carrier is involved in the transfer of electrons in the electron transport chain?

<p>FADH2 (B)</p> Signup and view all the answers

Which experiment provided early evidence that DNA is the genetic material?

<p>Griffith’s Experiment (B)</p> Signup and view all the answers

How does the integrity of the electron transport chain affect ATP production?

<p>It influences the proton gradient. (A)</p> Signup and view all the answers

What does extrachromosomal inheritance refer to?

<p>Genetic transmission that does not follow Mendelian principles. (A)</p> Signup and view all the answers

What is the role of chloroplast inheritance in plants?

<p>It involves maternal inheritance patterns. (D)</p> Signup and view all the answers

What is the primary role of bioenergy carriers within cells?

<p>To store and transfer energy (B)</p> Signup and view all the answers

What is the major focus of the Second Law of Thermodynamics in biological systems?

<p>Energy transformations increase entropy. (B)</p> Signup and view all the answers

How do mutations in DNA influence traits?

<p>They can create variations that are beneficial, neutral, or harmful. (C)</p> Signup and view all the answers

What is defined as the capacity to do work?

<p>Energy (B)</p> Signup and view all the answers

What is the expected outcome of alleles during gamete formation according to Mendel's law of segregation?

<p>Each gamete receives only one allele from each pair. (B)</p> Signup and view all the answers

In which process do hydrogen atoms play a more common role than single electron transfer?

<p>Redox reactions (A)</p> Signup and view all the answers

Which of the following processes occurs in mitochondria?

<p>Oxidative phosphorylation (C)</p> Signup and view all the answers

What is the significance of case studies on anaerobic respiration?

<p>They provide insights into alternative energy pathways. (B)</p> Signup and view all the answers

Which form of energy is specifically stored in molecular bonds?

<p>Chemical energy (D)</p> Signup and view all the answers

What are the two categories of nitrogenous bases found in nucleotides?

<p>Purines and pyrimidines (C)</p> Signup and view all the answers

Which enzyme is responsible for synthesizing new DNA strands during replication?

<p>DNA polymerase (C)</p> Signup and view all the answers

In which direction does DNA polymerase synthesize new DNA strands?

<p>5' to 3' (A)</p> Signup and view all the answers

What is the structure formed during the unwinding of DNA at the replication site?

<p>Replication fork (B)</p> Signup and view all the answers

Which component is NOT part of a nucleotide?

<p>Amino group (B)</p> Signup and view all the answers

What occurs during the elongation stage of DNA replication?

<p>Nucleotides are added to the growing strand (C)</p> Signup and view all the answers

What is the role of ligase during DNA replication?

<p>Joins Okazaki fragments (A)</p> Signup and view all the answers

Which RNA type is primarily involved in the synthesis of proteins?

<p>Messenger RNA (mRNA) (C)</p> Signup and view all the answers

What is the significance of the Meselson and Stahl experiment?

<p>It proved the semiconservative mechanism of DNA replication. (A)</p> Signup and view all the answers

What defines exons in gene structure?

<p>Coding regions that form mature mRNA (C)</p> Signup and view all the answers

Which of these processes occurs during transcription?

<p>DNA unwinds and RNA polymerase synthesizes RNA (B)</p> Signup and view all the answers

What is one key difference between DNA and RNA?

<p>RNA is typically single-stranded and contains uracil (D)</p> Signup and view all the answers

Which type of RNA forms structural components of ribosomes?

<p>Ribosomal RNA (rRNA) (C)</p> Signup and view all the answers

Which base pairs specifically in DNA?

<p>Adenine with Thymine (D)</p> Signup and view all the answers

What is the primary result of DNA replication?

<p>Two identical double helixes, each containing one old and one new strand (D)</p> Signup and view all the answers

What is the main function of the terminator in transcription?

<p>To release the RNA strand from the transcription complex (A)</p> Signup and view all the answers

During DNA replication, what is the role of DNA polymerase?

<p>To form new complementary strands by linking nucleotides (A)</p> Signup and view all the answers

In eukaryotic cells, RNA splicing is crucial because it removes which sequences from pre-mRNA?

<p>Introns (B)</p> Signup and view all the answers

In eukaryotic cells, how does DNA replication differ from that in prokaryotic cells?

<p>Eukaryotes have multiple origins of replication, while prokaryotes have one (B)</p> Signup and view all the answers

What is a key feature of the semi-conservative model of DNA replication?

<p>Each resulting DNA molecule retains one original strand (B)</p> Signup and view all the answers

What does RNA polymerase utilize as a template during transcription?

<p>The template strand of DNA (C)</p> Signup and view all the answers

Which of the following correctly describes the function of a 5' cap in eukaryotic mRNA?

<p>It protects mRNA from degradation (C)</p> Signup and view all the answers

Which enzyme is responsible for joining DNA pieces together during replication?

<p>DNA ligase (B)</p> Signup and view all the answers

How does eukaryotic transcription differ from prokaryotic transcription in terms of initiation?

<p>Eukaryotic transcription requires multiple general transcription factors (B)</p> Signup and view all the answers

What process follows transcription in gene expression?

<p>Translation (B)</p> Signup and view all the answers

What type of RNA is primarily transcribed by RNA polymerase II?

<p>mRNA (C)</p> Signup and view all the answers

Where does transcription occur in eukaryotic cells?

<p>In the nucleus (C)</p> Signup and view all the answers

Which component is NOT found in a structural gene as a transcriptional unit?

<p>Codons (A)</p> Signup and view all the answers

During transcription elongation, RNA is synthesized in which direction?

<p>5' to 3' (D)</p> Signup and view all the answers

What is the function of mRNA in gene expression?

<p>To encode genetic information for protein synthesis (B)</p> Signup and view all the answers

Which sequence is recognized for polyadenylation during transcription termination in eukaryotes?

<p>AAUAAA (A)</p> Signup and view all the answers

What is a significant feature of intrinsic (Rho-independent) termination?

<p>It forms a hairpin structure in RNA (D)</p> Signup and view all the answers

Which molecules are synthesized during transcription?

<p>RNA (D)</p> Signup and view all the answers

What role do introns play in eukaryotic gene expression?

<p>They regulate gene expression and contribute to diversity (B)</p> Signup and view all the answers

What are Okazaki fragments?

<p>Segments formed during the slow synthesis of the lagging strand (C)</p> Signup and view all the answers

Which structure contains the necessary components to initiate mRNA splicing in eukaryotes?

<p>Spliceosome (B)</p> Signup and view all the answers

What is the primary role of the promoter in a gene?

<p>To serve as the binding site for RNA polymerase (D)</p> Signup and view all the answers

Which of the following correctly describes the flow of genetic information according to the central dogma?

<p>DNA → RNA → Protein (C)</p> Signup and view all the answers

Which codon is recognized as the start signal for translation?

<p>AUG (D)</p> Signup and view all the answers

What does the term 'degeneracy' in the genetic code refer to?

<p>One amino acid can be coded by multiple codons (A)</p> Signup and view all the answers

What is a distinct feature of gene expression in prokaryotic cells compared to eukaryotic cells?

<p>Fast processing due to lack of transcription factors (B)</p> Signup and view all the answers

What is included in the structure of a fully processed eukaryotic mRNA?

<p>5' cap, coding region, 3' poly-A tail (B)</p> Signup and view all the answers

What is the main function of the cytoskeleton in a cell?

<p>To provide structural integrity and support cellular movement (B)</p> Signup and view all the answers

Which type of junctions prevent fluid leakage between animal cells?

<p>Tight junctions (D)</p> Signup and view all the answers

What distinguishes the smooth endoplasmic reticulum from the rough endoplasmic reticulum?

<p>Its function in lipid synthesis and carbohydrate metabolism (C)</p> Signup and view all the answers

What is the primary role of the Golgi apparatus in the cell?

<p>Modification and packaging of proteins (B)</p> Signup and view all the answers

In which phase of meiosis does crossing over occur?

<p>Prophase I (A)</p> Signup and view all the answers

What are the main products of cellular respiration?

<p>ATP and carbon dioxide (B)</p> Signup and view all the answers

Which structure is responsible for lipid metabolism and detoxification within a cell?

<p>Peroxisomes (B)</p> Signup and view all the answers

What is the term for the reproductive cells with half the genetic material?

<p>Gametes (D)</p> Signup and view all the answers

Which type of transport mechanism requires energy to move substances against a concentration gradient?

<p>Active transport (D)</p> Signup and view all the answers

What feature of the plasma membrane contributes to its selective permeability?

<p>Phospholipid bilayer structure (C)</p> Signup and view all the answers

What is the outcome of meiosis II in terms of chromosome number?

<p>Four haploid cells are produced (A)</p> Signup and view all the answers

What is a primary function of the plasma membrane?

<p>Regulate the internal environment of the cell (C)</p> Signup and view all the answers

What is the composition of ribosomes?

<p>Ribosomal RNA and proteins (C)</p> Signup and view all the answers

What occurs during Prophase I of meiosis that contributes to genetic diversity?

<p>Formation of a synaptonemal complex (C)</p> Signup and view all the answers

Which mechanism primarily causes genetic variation through random distribution of chromosomes?

<p>Independent assortment (B)</p> Signup and view all the answers

What is the main product of meiosis?

<p>Four haploid cells (A)</p> Signup and view all the answers

Which phase of meiosis involves the formation of tetrads?

<p>Prophase I (C)</p> Signup and view all the answers

How does crossing over enhance genetic diversity?

<p>By exchanging genetic material between homologous chromosomes (A)</p> Signup and view all the answers

What is the primary difference between meiosis and mitosis?

<p>Meiosis results in the production of gametes, while mitosis does not (C)</p> Signup and view all the answers

What is the site of crossing over called?

<p>Chiasmata (B)</p> Signup and view all the answers

Which phase of meiosis directly follows Anaphase I?

<p>Telophase I (D)</p> Signup and view all the answers

What ensures that each gamete receives a unique set of chromosomes?

<p>Independent assortment (D)</p> Signup and view all the answers

What is one consequence of genetic variation for species survival?

<p>It allows populations to adapt to changing environments (B)</p> Signup and view all the answers

What describes a bivalent in the context of meiosis?

<p>Two homologous chromosomes aligned together (B)</p> Signup and view all the answers

What is significant about the stages of Meiosis II?

<p>It resembles mitosis (D)</p> Signup and view all the answers

Which of the following statements about genetic recombination is true?

<p>It is the result of crossing over during Prophase I (A)</p> Signup and view all the answers

How does meiosis prevent chromosome doubling?

<p>By reducing chromosome number from diploid to haploid (D)</p> Signup and view all the answers

What primary function do mitochondria serve in eukaryotic cells?

<p>Cellular respiration (B)</p> Signup and view all the answers

Which component of plant cells is responsible for capturing sunlight for photosynthesis?

<p>Chloroplasts (A)</p> Signup and view all the answers

Which of the following structures is unique to prokaryotic cells?

<p>Cell wall (B)</p> Signup and view all the answers

Which of the following accurately describes the function of the endoplasmic reticulum (ER)?

<p>Protein and lipid synthesis (B)</p> Signup and view all the answers

What characteristic of eukaryotic cells allows them to perform more complex functions compared to prokaryotic cells?

<p>Nucleus and membrane-bound organelles (D)</p> Signup and view all the answers

Which structure contains the majority of a cell's genetic material in eukaryotic cells?

<p>Nucleus (A)</p> Signup and view all the answers

Which of the following best summarizes the Cell Theory?

<p>All living organisms are composed of cells. (D)</p> Signup and view all the answers

What is the primary role of the cytoskeleton in cells?

<p>Providing structural support and organization (C)</p> Signup and view all the answers

What occurs during the G1 phase of the eukaryotic cell cycle?

<p>Cellular growth and preparation for DNA replication occur. (A)</p> Signup and view all the answers

How do prokaryotic and eukaryotic cell cycles primarily differ?

<p>Eukaryotic cells have linear chromosomes. (A)</p> Signup and view all the answers

Which of the following structures is formed during cytokinesis in plant cells?

<p>Cell plate (D)</p> Signup and view all the answers

What is the significance of the Miller-Urey experiment?

<p>It simulated early Earth conditions leading to organic compound formation. (A)</p> Signup and view all the answers

Which type of reaction releases energy according to Gibbs free energy concepts?

<p>Exergonic reactions (A)</p> Signup and view all the answers

What can result from disruptions in the cell cycle?

<p>Uncontrolled cell division, leading to cancer (C)</p> Signup and view all the answers

What role do kinetochores play during mitosis?

<p>Attach spindle fibers to chromosomes (C)</p> Signup and view all the answers

Which of the following accurately describes aerobic respiration compared to anaerobic respiration?

<p>It is more efficient than anaerobic respiration. (A)</p> Signup and view all the answers

What is a key feature of both mitochondria and chloroplasts supporting the endosymbiotic theory?

<p>Existence of double membranes and circular DNA (B)</p> Signup and view all the answers

What does the second law of thermodynamics state?

<p>Energy transformations increase entropy. (C)</p> Signup and view all the answers

Which of the following is a characteristic of viruses?

<p>They lack metabolic processes. (D)</p> Signup and view all the answers

Which phase of mitosis involves the alignment of chromosomes at the metaphase plate?

<p>Metaphase (D)</p> Signup and view all the answers

What is the main purpose of ATP in cellular processes?

<p>To function as the primary energy carrier (A)</p> Signup and view all the answers

During which phase does the nuclear envelope break down?

<p>Prometaphase (D)</p> Signup and view all the answers

What is the primary purpose of cyclic electron flow in photosynthesis?

<p>To generate surplus ATP without NADPH or oxygen (D)</p> Signup and view all the answers

During which phase of the Calvin cycle does carbon dioxide fixation occur?

<p>Carbon Fixation (A)</p> Signup and view all the answers

Which molecule acts as the final electron acceptor in photophosphorylation?

<p>NADP+ (B)</p> Signup and view all the answers

What is the primary role of the thylakoid membranes in chloroplasts?

<p>Site for light energy absorption and electron transport (B)</p> Signup and view all the answers

What by-product is released during the water-splitting process in PSII?

<p>Oxygen (C)</p> Signup and view all the answers

How many ATP and NADPH molecules are consumed to convert 3-PGA into G3P during the Calvin cycle?

<p>2 ATP and 2 NADPH (B)</p> Signup and view all the answers

What defines the difference between photophosphorylation and oxidative phosphorylation?

<p>All of the above (D)</p> Signup and view all the answers

What is the main product of the Calvin cycle that can be used to form sugars?

<p>G3P (C)</p> Signup and view all the answers

Which of the following statements about the light reactions of photosynthesis is true?

<p>They involve the absorption of light and produce ATP and NADPH. (C)</p> Signup and view all the answers

In oxidative phosphorylation, what is the final electron acceptor?

<p>Oxygen (C)</p> Signup and view all the answers

What process occurs simultaneously with ATP synthesis in photophosphorylation?

<p>Reduction of NADP+ to NADPH (B)</p> Signup and view all the answers

Which enzyme plays a crucial role in the carbon fixation step of the Calvin cycle?

<p>Rubisco (C)</p> Signup and view all the answers

What is the significance of the proton gradient created during the light reactions?

<p>It drives the synthesis of ATP. (C)</p> Signup and view all the answers

Which process involves the direct transfer of a phosphate group from a substrate to ADP?

<p>Substrate-Level Phosphorylation (A)</p> Signup and view all the answers

What is the main bioenergy carrier in cells?

<p>ATP (B)</p> Signup and view all the answers

Which enzyme is responsible for introducing nicks in DNA to relieve tension ahead of the replication fork?

<p>Topoisomerase (A)</p> Signup and view all the answers

In eukaryotic cells, where does transcription take place?

<p>Nucleus (B)</p> Signup and view all the answers

What is the primary role of RNA polymerase during transcription?

<p>Synthesize RNA from the DNA template (B)</p> Signup and view all the answers

What are the short DNA segments synthesized on the lagging strand called?

<p>Okazaki Fragments (C)</p> Signup and view all the answers

Which of the following processes is central to oxidative phosphorylation?

<p>Electron Transport Chain (A)</p> Signup and view all the answers

According to the One Gene, One Enzyme hypothesis, what does each gene control?

<p>The synthesis of a specific enzyme (A)</p> Signup and view all the answers

What role do key electron carriers play in ATP production?

<p>They facilitate the transfer of electrons. (A)</p> Signup and view all the answers

What happens during the splicing process in eukaryotic cells?

<p>Introns are removed from RNA. (C)</p> Signup and view all the answers

What is the primary function of DNA ligase?

<p>Join Okazaki fragments (A)</p> Signup and view all the answers

What is the purpose of the promoter in a gene?

<p>To signal where transcription starts (B)</p> Signup and view all the answers

Which of the following statements accurately describes the genetic code?

<p>It is composed of codons that specify amino acids. (B)</p> Signup and view all the answers

Which term describes the flow of genetic information from DNA to RNA to protein?

<p>Gene Expression (D)</p> Signup and view all the answers

What is the primary role of ribosomal RNA (rRNA) in ribosomes?

<p>It catalyzes peptide bond formation. (C)</p> Signup and view all the answers

Which of the following mutations results in a premature stop codon?

<p>Nonsense mutation (C)</p> Signup and view all the answers

What is the primary structure responsible for synthesizing proteins in cells?

<p>Ribosome (D)</p> Signup and view all the answers

In which phase of cellular respiration does glycolysis occur?

<p>Cytoplasm (D)</p> Signup and view all the answers

Which component of photosynthesis is responsible for absorbing light energy?

<p>Chlorophyll (C)</p> Signup and view all the answers

What is released during the water-splitting process in photosynthesis?

<p>Oxygen (B)</p> Signup and view all the answers

Which of the following accurately describes the function of the E site in the ribosome?

<p>It serves as the exit site for tRNA. (C)</p> Signup and view all the answers

What is the significance of chlorophyll a in photosynthesis?

<p>It initiates the light-dependent reactions. (D)</p> Signup and view all the answers

What drives the synthesis of ATP during chemiosmosis in photosynthesis?

<p>The proton gradient across the thylakoid membrane (A)</p> Signup and view all the answers

What is the role of release factors during translation termination?

<p>To promote ribosomal subunit dissociation (D)</p> Signup and view all the answers

Which of the following statements is true regarding noncyclic electron flow?

<p>It involves both PS II and PS I. (D)</p> Signup and view all the answers

What energy conversion process occurs in chloroplasts?

<p>Photosynthesis (B)</p> Signup and view all the answers

What is required for the process of aminoacylation of tRNA?

<p>ATP (B)</p> Signup and view all the answers

Flashcards

Biology Definition

The science of studying living things (plants, animals, humans).

Cell Theory

All living things are made of cells, cells are the basic units, and cells come from pre-existing cells.

Prokaryotic Cell

A simple cell lacking a nucleus and organelles. DNA is in the cytoplasm.

Eukaryotic Cell

A complex cell with a nucleus and membrane-bound organelles.

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Cell's basic features

Plasma membrane, DNA, and ribosomes are found in all types of cells.

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Unicellular Organisms

Living things made of only one cell.

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Multicellular organism

Organisms composed of multiple cells.

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Cell Theory Key Figures

Schleiden, Schwann, and Virchow developed the Cell Theory in the 19th century.

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Bulk Transport

A process where cells move large molecules or particles across their membrane using vesicles.

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Exocytosis

The process of releasing substances from a cell by fusing vesicles with the cell membrane.

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Endocytosis

The process of taking substances into a cell by forming vesicles from the cell membrane.

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Phagocytosis

A type of endocytosis where cells engulf large particles, like bacteria, by wrapping their membrane around them.

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Pinocytosis

A type of endocytosis where cells take in small droplets of fluid by forming tiny vesicles.

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Receptor-mediated endocytosis

A specific type of endocytosis where cells take in specific molecules by binding to receptors on the membrane.

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Meiosis

A specialized cell division that reduces the chromosome number by half, creating gametes (sperm and eggs).

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Gametes

Reproductive cells (sperm and eggs) that carry half the genetic information of an organism.

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Zygote

The fertilized egg formed when two gametes (sperm and egg) unite, restoring the diploid chromosome number.

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Haploid

A cell with half the number of chromosomes (n), typically found in gametes.

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Crossing Over

The exchange of genetic material between homologous chromosomes during Prophase I of meiosis.

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Independent Assortment

The random orientation of homologous chromosome pairs during Metaphase I of meiosis.

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Bivalent

A pair of homologous chromosomes that come together during meiosis.

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Meiosis I vs. Meiosis II

Meiosis I separates homologous chromosomes, reducing chromosome number by half. Meiosis II separates sister chromatids, producing four haploid cells.

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Genetic Variation

Differences in genes within a population, caused by crossing over and independent assortment.

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Free Ribosomes

Ribosomes that are not attached to any membranes, found in the cytoplasm of eukaryotic cells. They mainly synthesize proteins destined for use within the cell.

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Attached Ribosomes

Ribosomes attached to the endoplasmic reticulum (ER) in eukaryotic cells. They produce proteins designed for export or to become part of the cell membrane.

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Endomembrane System

A network of interconnected internal membranes in eukaryotic cells, including the nuclear envelope, ER, Golgi apparatus, lysosomes, vacuoles, and plasma membrane. It regulates protein synthesis, modification, and transport within the cell.

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Mitochondria

Organelles in eukaryotic cells responsible for cellular respiration, which produces ATP for energy. They have their own DNA and ribosomes.

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Cytoskeleton

A network of protein fibers in eukaryotic cells that provides structural support, helps with cell movement, and organizes cellular activities.

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Lysosomes

Small, membrane-bound organelles in animal cells containing enzymes that break down old or unwanted cellular components.

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Centrioles

Small, cylindrical structures found in animal cells, involved in cell division and the formation of microtubules.

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Flagella

Long, whip-like structures used for locomotion in some eukaryotic cells.

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Chloroplasts

Organelles in plants and algae responsible for photosynthesis, converting light energy into chemical energy (glucose).

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Central Vacuole

A large, fluid-filled sac in plant cells that stores water, nutrients, and waste products. It also helps maintain cell shape and pressure.

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Tonoplast

The membrane that surrounds the central vacuole in plant cells, regulating the passage of molecules in and out.

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Cell Wall

A rigid outer layer found in plant, fungal, and bacterial cells, providing structural support and protection.

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Nucleus

The control center of a eukaryotic cell, containing most of the cell's DNA and regulating gene expression.

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Nuclear Envelope

A double membrane that surrounds the nucleus in eukaryotic cells, regulating the passage of molecules into and out of the nucleus.

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Nuclear Pore

Small openings in the nuclear envelope that allow the passage of specific molecules between the nucleus and cytoplasm.

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Nucleolus

A dense region within the nucleus of eukaryotic cells, responsible for assembling ribosomes.

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Synapsis

The pairing of homologous chromosomes during Prophase I, allowing for alignment and crossing-over.

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Chiasmata

The physical points of contact between homologous chromosomes where crossing-over occurs.

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Reduction Division

Meiosis reduces the number of chromosomes from diploid to haploid, ensuring genetic stability across generations.

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Genetic Recombination

The shuffling of genetic material from different strands of DNA, leading to novel genetic combinations.

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Prophase I

The longest phase of meiosis I, characterized by chromosome condensation, synapsis, and crossing-over.

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Metaphase I

The phase where tetrads align at the metaphase plate, spindle fibers attach, and homologous chromosomes prepare to separate.

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Anaphase I

The phase where homologous chromosomes are pulled apart to opposite poles, while sister chromatids remain attached, reducing the chromosome number.

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Telophase I

The phase where two haploid daughter cells are formed, each with replicated chromosomes, and cytokinesis occurs.

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Prophase II

The phase where a new spindle apparatus forms, and chromosomes move toward the metaphase plate, preparing for the second division.

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Metaphase II

The phase where sister chromatids align at the metaphase plate, with kinetochores attached to microtubules from opposite poles.

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Recombination

The process of mixing genetic material from different DNA strands, increasing diversity and contributing to evolution.

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What is the cell cycle?

A series of events leading to cell division, including growth, DNA replication, and chromosome distribution.

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G1 phase

The cell grows, synthesizes proteins for DNA replication, and performs its normal functions.

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S phase

DNA replication occurs, creating sister chromatids for each chromosome, doubling the genetic material.

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G2 phase

The cell continues to grow, replicates organelles, and makes proteins needed for mitosis, ensuring proper DNA.

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M phase

Mitosis and cytokinesis occur, resulting in two identical daughter cells.

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What are the key structures in cell division?

The spindle apparatus, centrosomes, kinetochores, cleavage furrow (animal), and cell plate (plant).

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Spindle apparatus

Made of microtubules, it separates sister chromatids during cell division.

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Why are checkpoints important?

Checkpoints ensure proper division and function, preventing errors that can lead to cancer.

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Prometaphase

The nuclear envelope breaks down, allowing spindle fibers to attach to chromosomes.

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What are the key structures in mitosis?

The spindle apparatus, centrosomes, and kinetochores.

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Aerobic Respiration

A process where organisms use oxygen to break down glucose, producing energy (ATP) more efficiently than anaerobic processes.

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Oxygenic Photosynthesis

A process where organisms, primarily cyanobacteria, use sunlight, water, and carbon dioxide to produce oxygen and sugar, releasing oxygen into the atmosphere.

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Endosymbiotic Theory

The theory that eukaryotic cells evolved through a symbiotic relationship where ancestral eukaryotic cells engulfed prokaryotic cells, explaining the origin of mitochondria and chloroplasts.

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Kinetic Energy

The energy of motion, like moving water or molecules within a cell.

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Potential Energy

Stored energy that has the potential to do work, like chemical bonds or water behind a dam.

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Chemical Energy

Energy stored in molecular bonds, released during chemical reactions to drive metabolic processes.

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First Law of Thermodynamics

Energy cannot be created or destroyed, only transformed or transferred within a closed system.

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Second Law of Thermodynamics

Energy transformations increase disorder (entropy) in a system over time, leading to spontaneous energy flow from organized to disorganized states.

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Anabolism

Metabolic processes that build complex molecules from simpler ones, requiring energy input.

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Catabolism

Metabolic processes that break down complex molecules into simpler ones, releasing energy.

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Gibbs Free Energy

The energy available to perform work in a system at constant temperature and pressure, determining a reaction's spontaneity.

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Exergonic Reaction

A reaction that releases energy, indicated by a negative change in Gibbs free energy (ΔG).

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Endergonic Reaction

A reaction that requires energy input, indicated by a positive change in Gibbs free energy (ΔG).

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Substrate-level phosphorylation

Direct transfer of a phosphate group from a phosphorylated molecule to ADP, generating ATP quickly.

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Chemiosmosis

ATP synthesis coupled with electron transport and proton gradient across a membrane.

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Oxidative phosphorylation

ATP production driven by electron transfer to oxygen in mitochondria during cellular respiration.

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Photophosphorylation

ATP production powered by light energy in chloroplasts during photosynthesis.

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Redox reaction

Transfer of electrons between substances, involving oxidation (loss of electrons) and reduction (gain of electrons).

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Electron carriers

Molecules that transport electrons in metabolic pathways, examples include NAD+, NADP+, FAD+, cytochromes, and quinones.

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Entropy

Measure of disorder or randomness in a system.

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ATP

Adenosine triphosphate, the primary energy currency of cells, used to power various cellular processes.

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Energy transfer

Movement of energy between different forms or systems.

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Gibbs Free Energy (G)

Maximum energy available to do work in a system. Helps predict spontaneity and feasibility of reactions.

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Energy Coupling

Using energy released from exergonic reactions to drive endergonic reactions.

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Activation Energy (EA)

Minimum energy required for reactants to undergo a chemical reaction. Acts as a barrier.

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Enzymes

Biological catalysts that speed up chemical reactions by lowering activation energy.

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Lock-and-Key Model

Describes enzyme specificity, where only a specific substrate can fit into the enzyme's active site.

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Active Site

Specific region on an enzyme where substrate binding occurs, leading to catalysis and product formation.

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Cofactors and Coenzymes

Non-protein molecules that assist enzymes in catalyzing reactions, enhancing functionality and specificity.

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Competitive Inhibition

Inhibitor competes with the substrate for the active site, reducing enzyme efficiency.

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Non-Competitive Inhibition

Inhibitor binds to a different site on the enzyme, changing its shape and impacting activity.

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Optimal Conditions for Enzymes

Specific temperature and pH levels where enzymes function most efficiently.

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Hydrogen Atom Transfer

Crucial for energy transfer in biological systems, facilitating electron movement, which is essential for energy production.

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Cellular Respiration

A metabolic pathway that releases energy from glucose through a series of oxidation reactions.

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Cellular Redox Homeostasis

The balance between oxidation and reduction reactions within cells, maintained by electron carriers.

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Chloroplasts: Photosynthesis

Organelles that capture light energy and convert it to chemical energy in glucose, sustaining plant life.

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Mitochondria: Cellular Respiration

Organelles responsible for breaking down glucose to produce ATP, the energy currency of the cell.

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Genetics

The study of heredity and how traits are passed from parents to offspring.

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Pangenesis

Early theory suggesting that particles from all body parts contribute to offspring.

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Aristotle's Vital Heat Theory

Theory proposing that offspring are formed from blood and influenced by a vital heat.

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Mendel's Laws of Inheritance

Principles describing how traits are passed from parents to offspring, including the law of segregation and the law of independent assortment.

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Dominant vs. Recessive Alleles

Alleles that determine the expression of traits, with dominant alleles masking recessive ones.

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Punnett Square

A tool used to predict the genotype and phenotype ratios of offspring based on parental genotypes.

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Hippocrates: Father of Medicine

Early physician who established a systematic approach to medicine, emphasizing observation and documentation.

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Aristotle: Classification and Reproduction Theory

Early philosopher who contributed to biology by classifying organisms and proposing early theories of reproduction.

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William Harvey: Epigenesis

Proposed that organisms develop from substances in the egg, challenging the preformation theory.

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Fixity of Species

Linnaeus's theory that species are immutable and created independently.

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Inheritance of Acquired Characters

Lamarck's theory suggesting that traits acquired during an organism's lifetime could be passed on.

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Law of Segregation

During gamete formation, each gamete receives only one allele from each pair, ensuring genetic diversity in offspring. This principle explains how traits are passed from parents to offspring.

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Dihybrid Cross

A cross between two individuals that differ in two traits. The expected phenotypic ratio is 9:3:3:1, representing different combinations of the two traits.

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Linkage

Genes located close together on the same chromosome tend to be inherited together. This can result in deviations from the expected phenotypic ratios in dihybrid crosses.

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Recombinant Gametes

Gametes that have received genetic material from both parents due to crossing over, leading to greater genetic diversity.

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Linkage Map

A diagram showing the relative position of genes on a chromosome, based on recombination frequencies.

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Sex Chromosomes

Chromosomes that determine an individual's sex. In humans, females are XX, and males are XY.

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X-linked Recessive Disorders

Conditions that are caused by recessive alleles located on the X chromosome. Males are more likely to be affected because they only have one X chromosome.

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Maternal inheritance

The transmission of genetic material from mother to offspring, independent of the father's contribution. This often involves extranuclear DNA, like mitochondrial DNA.

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Mitochondrial DNA

DNA located in mitochondria, inherited maternally. It plays a role in energy metabolism and certain diseases.

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Chloroplast Inheritance

Inheritance of chloroplasts, organelles responsible for photosynthesis, which typically follows a maternal pattern.

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Allele

Different forms of a gene found at the same locus on homologous chromosomes. These variations contribute to phenotypic differences in traits.

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Transcription

The process of copying genetic information from DNA into RNA.

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Transcription factors (TFs)

Proteins that bind to specific DNA sequences (promoters) and regulate gene expression.

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Promoter region

A specific DNA sequence where transcription factors bind to initiate transcription.

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Template strand

The strand of DNA used as a template for RNA synthesis during transcription.

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RNA polymerase

An enzyme that catalyzes the synthesis of RNA from a DNA template.

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Terminator

A DNA sequence that signals the end of transcription.

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Exon

A coding sequence within a gene that remains in the mature mRNA after RNA processing.

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RNA splicing

The process of removing introns and joining exons in pre-mRNA to create mature mRNA.

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Spliceosome

A complex of proteins and RNA molecules that carry out RNA splicing.

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5' cap

A modified guanine nucleotide added to the 5' end of eukaryotic mRNAs.

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Poly-A tail

A string of adenine nucleotides added to the 3' end of eukaryotic mRNAs.

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Translation

The process of converting the genetic code in mRNA into a protein.

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Codon

A three-nucleotide sequence in mRNA that specifies a particular amino acid.

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Genetic code

The set of rules that relates codons in mRNA to amino acids in proteins.

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Nucleotides

The building blocks of nucleic acids, composed of a nitrogenous base, a five-carbon sugar (ribose or deoxyribose), and a phosphate group.

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Purines

A type of nitrogenous base with a double-ring structure, including Adenine (A) and Guanine (G).

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Semi-Conservative Replication

The process of DNA replication where each new DNA molecule contains one original strand and one newly synthesized strand, ensuring genetic continuity.

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DNA Helicase

An enzyme that unwinds the DNA double helix, creating a replication fork.

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Replication Fork

The point where the DNA double helix is unwound, allowing for the synthesis of new strands in both directions.

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Origins of Replication

Specific sites on DNA where replication begins, where the DNA unwinds and forms replication bubbles.

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Photograph 51

An X-ray diffraction image taken by Rosalind Franklin that revealed the helical structure of DNA.

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DNA Backbone

The structural framework of DNA, formed by alternating sugar (deoxyribose) and phosphate groups.

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Base Pairing

The specific pairing of nitrogenous bases in DNA (A-T, C-G), ensuring complementary strands.

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DNA Replication

The process by which a DNA molecule is copied to produce two identical DNA molecules.

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Meselson and Stahl Experiment

A classic experiment that proved DNA replication is semi-conservative by using heavy nitrogen isotopes to track DNA strands.

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Leading Strand

The DNA strand synthesized continuously in the 5' to 3' direction, following the replication fork.

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Lagging Strand

The DNA strand synthesized discontinuously in short fragments (Okazaki fragments) in the 5' to 3' direction, going against the replication fork.

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DNA Primase

An enzyme that synthesizes short RNA primers to initiate DNA replication, providing a starting point for DNA polymerase.

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Okazaki Fragments

Short DNA fragments synthesized on the lagging strand, later joined together by DNA ligase.

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Central Dogma

The fundamental principles of molecular biology describing the flow of genetic information from DNA to RNA to protein.

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Messenger RNA (mRNA)

A type of RNA that carries the genetic code from DNA to ribosomes for protein synthesis.

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What is the function of the nucleus?

The nucleus is the control center of the cell, containing most of the cell's DNA and regulating gene expression.

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What are ribosomes?

Ribosomes are small organelles that synthesize proteins, either free in the cytoplasm or attached to the endoplasmic reticulum.

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What is the endomembrane system?

A network of interconnected internal membranes including the nuclear envelope, ER, Golgi apparatus, lysosomes, vacuoles, and the plasma membrane.

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What is the function of mitochondria?

Mitochondria are organelles responsible for cellular respiration, producing ATP (energy) for the cell.

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Electron Microscope

A microscope that uses electrons to create highly detailed images, revealing structures much smaller than what light microscopes can show.

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Micrometers (µm)

A unit of length equal to one millionth of a meter, often used for measuring cell sizes.

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Plasma Membrane

A thin, flexible barrier that surrounds a cell, controlling what enters and exits.

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Cytoplasm

The gel-like substance inside a cell, containing organelles and where many metabolic reactions occur.

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Chromosomes

Structures made of DNA that contain genetic information passed down from parents to offspring.

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Endoplasmic Reticulum (ER)

A network of folded membranes involved in protein synthesis, modification, and lipid production.

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Golgi Apparatus

A stack of membranous sacs that modify, package, and sort proteins and other molecules for transport.

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Extracellular Matrix (ECM)

A complex network of molecules outside animal cells that provides structural support and helps cells communicate.

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Tight Junctions

Intercellular junctions that tightly seal the space between adjacent animal cells, preventing the leakage of fluids.

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Gap Junctions

Cell junctions that create small channels between adjacent animal cells, allowing for the passage of ions and small molecules.

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Tetrad

A structure formed during Prophase I of meiosis, consisting of four chromatids (two homologous chromosomes, each with two sister chromatids).

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Activation Energy

The minimum energy needed for reactants to start a chemical reaction.

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Cell Cycle

The series of events that a cell undergoes from its formation to its division into two daughter cells. It includes growth, DNA replication, and chromosome separation.

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Interphase

The longest phase of the cell cycle, where the cell grows and prepares for division. It includes G1, S, and G2 phases.

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Checkpoints

Control points in the cell cycle that ensure proper division and function. They prevent errors in DNA replication and chromosome segregation, preventing the formation of unhealthy daughter cells.

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Binary Fission

The method of cell division used by prokaryotes, where a single cell divides into two identical daughter cells. It's simpler than mitosis and doesn't involve a nucleus.

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Centrosome

An organelle that organizes microtubules and is crucial for spindle formation during mitosis and meiosis.

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Kinetochore

A protein structure that attaches to the centromere of a chromosome and helps to move chromosomes during cell division.

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Ribosome Structure

Ribosomes are made of two subunits (smaller and larger) and have three binding sites: A site (aminoacyl tRNA), P site (peptidyl tRNA), and E site (exit).

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Ribosome Function

Ribosomes are responsible for protein synthesis, reading mRNA codons and linking amino acids together.

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What is tRNA?

Transfer RNA (tRNA) carries specific amino acids to the ribosome and has an anticodon that pairs with the mRNA codon.

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Aminoacylation

The process of attaching (charging) an amino acid to tRNA, requiring energy (ATP) and specific enzymes.

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Gene Mutation

A permanent change in the DNA sequence that alters the genetic information.

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Missense Mutation

Changes one amino acid in the protein sequence, potentially altering its function.

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Nonsense Mutation

Creates a stop codon (UAA, UGA, or UAG) in the mRNA, leading to a truncated (shortened) protein.

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Frameshift Mutation

Insertion or deletion of nucleotides, shifting the reading frame and altering the amino acid sequence.

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Initiation (Translation)

The process of starting protein synthesis where the initiator tRNA binds to the ribosome and mRNA.

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Elongation (Translation)

The ribosome reads the mRNA codons one at a time, adding amino acids to the growing polypeptide chain.

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Termination (Translation)

The process of stopping protein synthesis when a stop codon is reached, releasing the completed polypeptide.

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Cellular Respiration: Energy Conversion

The process where glucose is broken down to generate ATP, the energy currency of cells, using oxygen.

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Photosynthesis: Light Energy Conversion

The process where light energy is used to convert carbon dioxide and water into glucose and oxygen.

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Mitochondria: ATP Production

Mitochondria are the 'powerhouses' of eukaryotic cells, where ATP is produced using aerobic respiration.

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Chloroplasts: Glucose Production

Chloroplasts are the sites of photosynthesis in plant and algal cells, converting light energy into chemical energy (glucose).

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Photosystem II (PSII)

A photosystem that absorbs light energy at 680 nm and uses it to split water molecules, releasing oxygen as a byproduct and providing electrons for the electron transport chain.

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Electron Transport Chain (ETC)

A series of protein complexes embedded in the thylakoid membrane that transfer electrons from PSII to PSI, using the energy released to pump protons across the membrane, creating a proton gradient.

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Photosystem I (PSI)

A photosystem that absorbs light energy at 700 nm and re-energizes electrons, transferring them to NADP+ reductase, ultimately producing NADPH.

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Calvin Cycle

A metabolic pathway that takes place in the stroma of chloroplasts, fixing carbon dioxide into organic molecules using ATP and NADPH produced in the light reactions.

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Rubisco

An enzyme that catalyzes the first step of the Calvin cycle, fixing carbon dioxide to ribulose bisphosphate (RuBP).

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G3P (Glyceraldehyde-3-phosphate)

A three-carbon sugar produced in the Calvin cycle, serving as a key building block for glucose, starch, and other organic molecules in plants.

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Cyclic Electron Flow

An alternative pathway involving only PSI, where excited electrons are recycled back to the cytochrome b6/f complex, generating additional ATP without producing NADPH or oxygen.

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ATP Synthase

A protein complex embedded in the thylakoid membrane (in chloroplasts) and the inner mitochondrial membrane (in mitochondria) that utilizes the proton gradient to synthesize ATP.

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Stroma

The fluid-filled region within the chloroplast that houses the Calvin cycle and other metabolic processes.

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Thylakoid Membrane

A system of internal membranes within the chloroplast, forming flattened sacs called thylakoids and interconnected compartments called grana, where the light reactions of photosynthesis occur.

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RuBP (Ribulose bisphosphate)

A five-carbon sugar that serves as the primary carbon acceptor in the Calvin cycle, binding to carbon dioxide to begin the fixation process.

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3-PGA (3-phosphoglycerate)

A three-carbon compound formed by the carboxylation of RuBP, ultimately converted to G3P in the Calvin cycle.

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Study Notes

Biology: Defining and Understanding Living Organisms

  • Biology is the study of living organisms (plants, animals, humans).
  • Derived from Greek roots: 'bio' (life) and 'logy' (study of).

Cell Theory

  • All living organisms are composed of cells.
  • Cells are the basic structural and functional units.
  • Cells arise from pre-existing cells.
  • Developed by Schleiden, Schwann, and Virchow in the 19th century.

Understanding Organisms

  • Organisms are living entities composed of molecules.
  • Unicellular (bacteria) consist of a single cell; multicellular (humans, plants, animals) are made of many.
  • 'Organum' (Latin) refers to organisms, highlighting their organized nature.

Cell Theory and Historical Discoveries

  • Cell theory: All organisms are made of one or more cells; cells are fundamental units.
  • Cells only originate from pre-existing cells.
  • Antoine van Leeuwenhoek observed microorganisms, enhancing biological understanding.
  • Robert Hooke (1665) discovered "cells" using microscopy.

Basic Features of Cells

  • Plasma membrane, DNA, and ribosomes are fundamental cell components.
  • DNA and RNA are vital for cell function, guiding protein synthesis.
  • Prokaryotic cells lack nuclei and organelles; eukaryotic cells are more complex, possessing these.
  • Cell shape and organization are adaptations to function.

Prokaryotic Cell Structure

  • Shapes: spherical, rod-like, spiral.
  • DNA: single circular molecule.
  • Cell wall: surrounds plasma membrane, coated with polysaccharides.
  • Flagella and pili aid in attachment.
  • Plasmids carry extra genes.
  • Cytoplasm supports essential functions.

Eukaryotic Cell Characteristics

  • Complex nucleus and membrane-bound organelles.
  • Transcription and translation occur in different compartments.
  • Lower surface area-to-volume ratio compared to prokaryotic cells affects interactions with environment.

Characteristics of Eukaryotic Cells

  • Cytoplasm contains membranous organelles for various cellular processes (metabolism, synthesis, storage).
  • Ribosomes exist free or attached to membranes.
  • Endomembrane system partitions cytoplasm.
  • Mitochondria are sites of cellular respiration; chloroplasts are in plant cells (photosynthesis).

Cytoskeleton in Eukaryotic Cells

  • A network of fibres (microtubules, microfilaments) supporting structure and cell movement.
  • Eukaryotic cells have evolved more supporting proteins.
  • Genes (specific roles) control cytoskeletal functions.
  • Nuclei contribute to eukaryotic complexity.

Plant vs Animal Cells

  • Animal cell organelles: lysosomes, centrioles, flagella.
  • Plant cell organelles: chloroplasts, central vacuole, cell wall.
  • Organelle differences reflect specific cell functions.

Eukaryotic Cell Nucleus Overview

  • Nucleus contains most of the cell's DNA.
  • Nuclear envelope separates nucleus from cytoplasm.
  • Nuclear pores regulate molecular transport.
  • Nucleolus assembles ribosomes.
  • Chromatin forms chromosomes.
  • Nuclear lamina: dense network of filaments and proteins.

Microscopy Overview

  • Bacteria size: ~0.5 μm; plant cells can be several hundred μm.
  • Units: micrometres (μm), nanometres (nm), angstroms (Å).

Common Cell Elements

  • Essential structures: plasma membrane, cytoplasm, chromosomes, ribosomes, cytoskeleton.
  • Plasma membrane's phospholipid bilayer controls substance flow.

Microscopy Techniques

  • Light microscopes: use light (bright field, differential interference contrast, fluorescence).
  • Electron microscopes: use electrons (transmission, scanning).

Ribosomes in Cells

  • Found in all living cells.
  • Composed of ribosomal RNAs (rRNAs) and proteins.
  • Site of protein synthesis (cytosol, outside of ER and nucleus).
  • Consist of large and small subunits.

Endomembrane System in Cells

  • Includes nuclear envelope, ER, Golgi apparatus, lysosomes, vacuoles, plasma membrane.
  • Regulates protein synthesis, modification, and transport.

Endoplasmic Reticulum in Eukaryotic Cells

  • Accounts for much of the cell's membrane.
  • Continuous with the nuclear envelope.
  • Two regions: smooth (no ribosomes) and rough (ribosomes).
  • Rough ER: protein modification/folding; Smooth ER: lipid synthesis/carbohydrate regulation.

Eukaryotic Cell Endomembrane System Overview

  • Golgi apparatus: flattened membranous sacs (cisternae).
  • Modifies ER products, manufactures macromolecules, sorts/packages materials into vesicles.
  • Maturing face (trans face) and forming face (cis face) for processing.

Mitochondria and Chloroplasts

  • Mitochondria perform cellular respiration (ATP production).
  • Chloroplasts (plants/algae): sites of photosynthesis.
  • Peroxisomes: oxidative organelles (lipid metabolism, detoxification).

Cytoskeleton and Cellular Movements

  • Network of fibres (microtubules, microfilaments).
  • Organizes cell structure and activities.

Extracellular Components and Connections

  • Cells synthesize and secrete materials (cell wall, ECM, junctions).

Intercellular Junctions Overview

  • Facilitate contact between cells.
  • Plant cells (plasmodesmata); Animal cells (tight junctions, gap junctions, desmosomes).

Plant Plasmodesmata

  • Membrane-lined pores for small molecule transport.
  • Intercellular communication and regulated trafficking.

Animal Intercellular Junctions

  • Tight junctions: prevent leakage; Desmosomes: anchor cells; Gap junctions: channels.
  • Functions: fluid control, structure, communication.

Fluid Mosaic Model

  • Plasma membrane's selective permeability.
  • Composed of lipids and proteins (fluid mosaic).

Element Descriptions (Plasma Membrane)

  • Phospholipids: form bilayer with hydrophobic/hydrophilic regions.
  • Membrane proteins: integral/peripheral, determine membrane function.
  • Membrane fluidity and cholesterol: maintain structure and fluidity at various temperatures.

Membrane Proteins

  • Integral proteins penetrate the membrane; peripheral proteins do not.
  • Functions: transport, enzymatic activity, signal transduction, cell recognition.

Passive Transport

  • Diffusion and facilitated diffusion (down concentration gradients).
  • No energy required.
  • Osmosis affects water balance with solute concentrations.

Active Transport

  • Moves substances against gradients, requires energy.
  • Types: primary (direct) and secondary (indirect) active transport.
  • Example: sodium-potassium pump.

Bulk Transport

  • Exocytosis: material export.
  • Endocytosis: material import (phagocytosis, pinocytosis, receptor-mediated).

Meiosis

  • Specialized cell division in sexually reproducing organisms.
  • Reduces chromosome number by half (gametes).
  • Crucial for maintaining chromosome stability across generations.

Meiosis: Genetic Diversity

  • Introduces genetic variety through recombination and independent assortment.
  • Four genetically distinct haploid cells from one diploid cell.
  • Essential for sexual reproduction (combining genetic material).

Stages of Meiosis

  • Two main stages: Meiosis I and Meiosis II; each has distinct phases.
  • Meiosis I: separates homologous chromosomes.
  • Meiosis II: separates sister chromatids (similar to mitosis).

Key Terminology

  • Gametes: reproductive cells (sperm, egg).
  • Zygote: fertilized egg, restoring diploid chromosomes.
  • Bivalent: homologous chromosome pair.
  • Tetrad: four chromatids (two homologous chromosomes).
  • Crossing Over: genetic material exchange.
  • Haploid (n): half the number of chromosomes.

Meiosis I Phases

  • Prophase I: condensation, synapsis, crossing over.
  • Metaphase I: bivalents align, spindle fibers attach.
  • Anaphase I: homologous chromosomes separate.
  • Telophase I/Cytokinesis: two haploid cells formed.

Meiosis II Phases

  • Prophase II: chromosomes condense, spindle reforms.
  • Metaphase II: chromosomes align, sister chromatids attach.
  • Anaphase II: sister chromatids separate.
  • Telophase II/Cytokinesis: four haploid cells produced.

Mechanisms of Genetic Variation

  • Crossing over during Prophase I (new allele combinations).
  • Independent assortment during Metaphase I (combinatorial variety).

Importance of Genetic Variation

  • Critical for natural selection and adaptation.
  • Drives species evolution.
  • Used in agriculture (crop resilience).
  • Aids in personalized medicine (genetic tailoring).

Cell Cycle Overview

  • Series of events in cell division (growth, DNA, distribution).
  • Four phases: G1, S, G2, M.
  • Interphase is the most time-consuming phase.
  • Tight regulation crucial for cell function.
  • Disruptions can cause uncontrolled growth (cancer).

Phases of Eukaryotic Cell Cycle

  • G1 Phase: cell growth/protein synthesis.
  • S Phase: DNA replication (sister chromatids).
  • G2 Phase: organelle duplication/protein synthesis.
  • M Phase: mitosis and cytokinesis.

Prokaryotic vs Eukaryotic Cell Cycles

  • Prokaryotes divide by binary fission, no mitosis.
  • Eukaryotes have complex cycles.

Key Structures in Cell Division

  • Spindle apparatus: microtubule arrangement, separates sister chromatids.
  • Centrosome: microtubule organizing center.
  • Kinetochore: protein complexes attaching chromosomes to spindles.

Key Characteristics of Living Organisms

  • Order: complex organization in living things.
  • Sensitivity: reactions to environmental stimuli.
  • Reproduction: species continuation.
  • Growth/Development: size/complexity change.
  • Regulation: internal balance maintenance.
  • Homeostasis: stable internal conditions.

Viruses

  • Not considered "living" organisms.
  • Cannot carry out metabolic processes independently.
  • Consist of protein coat (capsid) and genetic material (DNA/RNA).
  • Need a host to reproduce.

Theories on Life's Origins

  • Oparin-Haldane theory: organic molecules originated abiotically on early Earth, supported by external energy.
  • Miller-Urey experiment: synthesized organic compounds.
  • Hypotheses: reducing atmospheres, deep-sea vents, extraterrestrial origins.

Significance of Oxygenic Photosynthesis

  • Provided life with a light-and-water-based energy source.
  • Released oxygen, transforming Earth's atmosphere and oceans, enabling aerobic respiration.

The Rise of Oxygen

  • Enabled the evolution of aerobic respiration.
  • Reshaped biogeochemical cycles.
  • Increase in global primary productivity.
  • Led to the emergence of multicellular life and biodiversity.

Impacts on Ecosystems

  • Supported complex food webs.
  • Led to the formation of the ozone layer, shielding UV radiation.
  • Facilitated the Cambrian explosion.

Origin of Mitochondria and Chloroplasts

  • Endosymbiotic theory: eukaryotic cells evolved symbiotically.
  • Mitochondria and chloroplasts originated from prokaryotic cells.
  • Evidence: double membranes, distinct DNA, resemblance to bacteria.

Evidence Supporting Endosymbiosis

  • Molecular/genetic evidence supports the endosymbiotic theory (ribosomes, genes).

Understanding Energy

  • Energy: capacity to do work; various forms (kinetic, potential, chemical, thermal).

Laws of Thermodynamics

  • First Law: energy cannot be created or destroyed (only transformed).
  • Second Law: energy transformations increase entropy (disorder).
  • Foundation for energy flow in biological systems

Cellular Metabolism

  • All chemical activities within a cell.
  • Metabolic pathways (anabolism/catabolism).
  • Balance essential for homeostasis.

Gibbs Free Energy

  • Quantifies energy available for work at constant temperature/pressure.
  • ΔG: reaction spontaneity (negative ΔG = spontaneous).
  • Exergonic (releases energy); endergonic (requires energy).

Role of Enzymes

  • Biological catalysts, lowering activation energy.
  • Specificity (lock-and-key).
  • Influenced by pH, temperature, inhibitors.

Enzyme Cofactors and Coenzymes

  • Cofactors: inorganic ions (e.g., Fe, Cu).
  • Coenzymes: organic molecules (e.g., NAD, FAD); temporarily bound to enzymes.

ATP as Energy Currency

  • Primary energy carrier in cells.
  • Energy stored in chemical bonds.
  • Released via hydrolysis (ATP to ADP + Pi).

Mechanisms of ATP Generation

  • Substrate-level phosphorylation: direct phosphate transfer to ADP.
  • Chemiosmosis: ATP synthesis linked to electron transport.

Overview of Bioenergy Carriers

  • Key molecule: ATP (adenosine triphosphate).
  • Generated by phosphorylation (substrate-level/chemiosmosis).

Types of Phosphorylation

  • Oxidative phosphorylation: electron transfer to oxygen; crucial in mitochondria.
  • Photophosphorylation: light-driven synthesis; crucial in chloroplasts.

Redox Reactions in Energy Transfer

  • Redox: electron transfer (oxidation/reduction).
  • Essential in metabolic pathways (e.g., glycolysis, citric acid cycle).

Key Electron Carriers

  • NAD+/NADH; NADP+/NADPH; FAD/FADH2; cytochromes; quinones.
  • Crucial for electron transfer in metabolic pathways.

Key Structures in Energy Conversion

  • Chloroplasts (photosynthesis).
  • Mitochondria (cellular respiration).

Introduction to Genetics

  • Genetics: study of heredity and variation.
  • Historical development of genetic concepts.

Historical Perspectives on Genetics

  • Early theories about heredity and reproduction.
  • Contrasting views on inheritance.

Key Concepts in Mendelian Genetics

  • Mendel's laws: Segregation, Independent Assortment.
  • Alleles (dominant/recessive).
  • Punnett squares for predicting outcomes.

Gregor Mendel: The Father of Genetics

  • Austrian monk, pioneering experiments on pea plants.
  • Established fundamental genetic principles.

Mendel's Experiments

  • Monohybrid crosses: demonstrated trait segregation.
  • F1 and F2 generations: predictable phenotypic ratios.
  • Law of Segregation: alleles separate during gamete formation.

Variations and Extensions of Mendelian Genetics

  • Codominance: both traits expressed in offspring.
  • Multiple alleles: more than two alleles per gene.
  • Pleiotropy: single gene affecting multiple traits.

Chromosomal Basis of Inheritance

  • Genes located on chromosomes.
  • Homologous chromosomes.
  • Genetic variation from parental contributions.

Chromosomal Basis of Allele Segregation

  • Two alleles (identical/different), one from each parent.
  • Allele segregation during gamete formation.

Dihybrid Crosses

  • Phenotypic ratio: 9:3:3:1.
  • Deviation: linkage.

Crossing Over and Genetic Mapping

  • Crossing over in meiosis I (genetic recombination).
  • Morgan's experiments with Drosophila.
  • Linkage maps (genetic distances).

Bateson and Punnett's Experiments

  • Revealed non-independent trait assortment (linked genes).
  • Deviations from Mendelian expectations.

Sex Chromosomes and Determination

  • Sex determined by X and Y chromosomes (XX/XY).
  • Other systems (XO, ZW).

Disorders Associated with Sex-linked Inheritance

  • X-linked recessive disorders (hemophilia, color blindness).
  • Abnormal sex chromosome numbers.

Maternal Inheritance and Mitochondrial DNA

  • Extranuclear inheritance - maternal contribution.
  • Mitochondrial DNA inheritance, traits, diseases.

Chloroplast Inheritance and Epigenetics

  • Maternal inheritance in plants (Mirabilis Jalapa).
  • Epigenetic factors influence development.

Key Definitions

  • Allele; Gamete; Linkage.

Characteristics of Genetic Material

  • Replication; Information storage; Information expression; Variation through mutation.

Discovery of DNA as the Genetic Material

  • Early studies suggested DNA/protein in the nucleus.
  • DNA quantity/gamete halving: early DNA evidence.
  • Griffith's experiment (bacteria transformation).

Experiments Confirming DNA as Genetic Material

  • Griffith's experiment; Avery's experiment; Hershey-Chase experiment.

Nucleic Acid Structures

  • Nucleotide composition (base, sugar, phosphate).
  • Nitrogenous bases (purines/pyrimidines).
  • Nucleosides/nucleotides.

Mechanism of DNA Replication

  • Semi-conservative replication (one old strand, one new).
  • Enzymatic roles (DNA helicase, polymerase, ligase).
  • Replication Fork.

Stages of DNA Replication

  • Initiation: replication origin.
  • Elongation: adding nucleotides.
  • Termination: completed replication.

Gene Structure

  • Gene definition.
  • Promoters/enhancers.
  • Introns/exons.

Gene Expression

  • Transcription; Translation; Regulation.

Key Discoveries in DNA Structure

  • X-ray crystallography - Franklin's work.
  • Watson and Crick's double helix model.

Components of DNA

  • Nitrogenous bases (A, T, C, G).
  • Base pairing (A-T, C-G).
  • Sugar-phosphate backbone.

Comparison with RNA

  • RNA structure (single-stranded, ribose, uracil).
  • Types of RNA (mRNA, tRNA, rRNA).

Genetic Information Encoding

  • Linear base sequence and genetic code (codons).

Mechanisms of DNA Replication

  • Semi-conservative (Meselson-Stahl).

Key Enzymes and Proteins

  • DNA helicase, polymerase, ligase - functions.
  • Topoisomerase, single-strand binding proteins.

Replication Fork Dynamics

  • Leading strand; Lagging strand; Okazaki fragments.
  • 5' to 3' direction.

Replication Rates and Origins

  • Bacteria vs. eukaryotes (replication rates, origins).

DNA replication (various summaries):

  • Different points of view on DNA replication's mechanisms, origins, and rates.
  • Including tables/explanations of the semi-conservative mechanism, protein involvement, and origin locations.

Gene Expression (various summaries):

  • Various summaries detailing the process and comparing prokaryotes and eukaryotes, including protein roles, initiation, transcription, termination, and elongation.

Transcriptomic vs Epigenetic Inheritance

  • Various summaries about different inheritances and mechanisms of DNA control..

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This quiz explores the fundamental concepts of biology, focusing on the definition and understanding of living organisms. It covers cell theory, the historical contributions of scientists, and the distinctions between unicellular and multicellular organisms.

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