MICRO: Class 2  The Scientific Method and Nucleic Acids

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Questions and Answers

What is the first step in the scientific method?

  • Communicate methods, results, and conclusions
  • Design experiments to test the hypothesis
  • Draw a conclusion
  • Make an observation and ask a question (correct)

What is a hypothesis?

  • A testable explanation for a phenomenon (correct)
  • The final conclusion drawn from an experiment
  • A specific type of experiment
  • A statement of fact observed in nature

Why is a control included in experiments?

  • To ensure that all variables are accounted for
  • To rule out alternative explanations (correct)
  • To provide a visual representation of the data
  • To make the experiment more complex

What does the scientific method emphasize after analyzing data?

<p>Drawing a conclusion (A), Communicating the results (B)</p> Signup and view all the answers

What characterizes a scientific theory?

<p>An explanation supported by a large amount of evidence (D)</p> Signup and view all the answers

Which part of the scientific method can be refined based on experimental results?

<p>The hypothesis (B)</p> Signup and view all the answers

What is the importance of communicating methods and results in science?

<p>To allow replication and understanding by others (C)</p> Signup and view all the answers

What does the phrase 'every rule has exceptions' imply about scientific rules?

<p>Understanding exceptions can improve their utility (D)</p> Signup and view all the answers

What term describes spherical bacteria that may be slightly oval or flattened on one end?

<p>Coccus (A)</p> Signup and view all the answers

Which of the following terms refers to a long, spiral-shaped cell with a flexible cell wall?

<p>Spirochete (D)</p> Signup and view all the answers

What term is used for cocci that remain in pairs after division?

<p>Diplococci (A)</p> Signup and view all the answers

Which bacterial arrangement describes long chains formed by division in one plane?

<p>Streptococcus (B)</p> Signup and view all the answers

What characteristic defines bacteria that can appear in varying shapes?

<p>Pleomorphic (D)</p> Signup and view all the answers

What is the primary difference between Archaea and Bacteria?

<p>Ribosomal RNA sequences (C)</p> Signup and view all the answers

Which genus of bacteria is known for forming cubical packets after division in perpendicular planes?

<p>Sarcina (A)</p> Signup and view all the answers

Which type of bacteria often thrive in extreme environments, such as high salt and temperature?

<p>Archaea (D)</p> Signup and view all the answers

What is the correct order of information flow in cellular processes?

<p>DNA à RNA à Protein (C)</p> Signup and view all the answers

What significant event occurred around 2.5 billion years ago in Earth's history?

<p>Single-celled organisms began terraforming the planet (D)</p> Signup and view all the answers

What do the lengths of the lines in the Universal Tree of Cellular Life represent?

<p>Time of evolutionary changes (B)</p> Signup and view all the answers

Which of the following is NOT one of the four RNA bases?

<p>Thymine (B)</p> Signup and view all the answers

How old is the Earth estimated to be?

<p>4.6 billion years (B)</p> Signup and view all the answers

The simplest assembly of life's origins suggests that all cellular life evolved from what?

<p>A single-celled ancestor (C)</p> Signup and view all the answers

Which of the following statements best describes the process of evolution mentioned?

<p>It involves gradual changes over extensive time scales. (B)</p> Signup and view all the answers

What is a common misconception about mutations in DNA?

<p>They always lead to beneficial traits. (B)</p> Signup and view all the answers

What was the impact of the influenza pandemic of 1918 to 1919 compared to world wars?

<p>It killed more Americans than WWI, WWII, and the Korean, Vietnam, and Iraq wars combined. (C)</p> Signup and view all the answers

What factor contributed to the great famine in Ireland during the 1800s?

<p>A microbial disease of potatoes. (C)</p> Signup and view all the answers

What consequences did the disease affecting olive trees have?

<p>It caused a significant drop in olive oil production worldwide. (D)</p> Signup and view all the answers

What was the historical perception of diseases before the discovery of microorganisms?

<p>They were caused by the poison of bad air. (D)</p> Signup and view all the answers

Which disease devastated wheat crops and spread to various countries?

<p>Wheat blast. (A)</p> Signup and view all the answers

Which of the following methods was used in ancient times for food preservation?

<p>Fermentation (A)</p> Signup and view all the answers

What was the purpose of the sanitation instructions found in Deuteronomy 23:12-14?

<p>To ensure God did not dirty His shoes. (B)</p> Signup and view all the answers

Which of the following statements is true about smallpox?

<p>It was responsible for one-third of its victims dying. (C)</p> Signup and view all the answers

How is the current situation with the plague characterized?

<p>It is still present but not spreading among humans. (D)</p> Signup and view all the answers

What evolution-focused behavior do microorganisms display according to the content?

<p>They focus on surviving and reproducing. (D)</p> Signup and view all the answers

What misconception did ancient civilizations have about sanitation practices?

<p>They followed rituals without understanding their purpose. (B)</p> Signup and view all the answers

What remains a challenge in the vaccination efforts for polio?

<p>Religious and political barriers to reaching last human reservoirs. (D)</p> Signup and view all the answers

What recent emerging disease is mentioned alongside smallpox eradication?

<p>Monkeypox. (B)</p> Signup and view all the answers

Which of the following is NOT mentioned as an impact of microorganisms on humans today?

<p>Quantum computing (A)</p> Signup and view all the answers

In ancient medicine, what was the main approach to understanding diseases?

<p>Accurate descriptions of symptoms. (B)</p> Signup and view all the answers

Which of the following correctly describes the relationship between microorganisms and human diseases?

<p>Microorganisms are indifferent to human suffering. (A)</p> Signup and view all the answers

What structure do molds form that is visible to the naked eye?

<p>Mycelium (C)</p> Signup and view all the answers

Which of the following is characteristic of fungi?

<p>Opportunistic infections can occur (B)</p> Signup and view all the answers

In which part of algae does photosynthesis take place?

<p>Chloroplasts (D)</p> Signup and view all the answers

What is the role of enzymes secreted by fungi?

<p>To digest organic materials (C)</p> Signup and view all the answers

Which of the following is NOT true about algae?

<p>They are always found in dry habitats. (D)</p> Signup and view all the answers

What type of infections can fungi cause?

<p>Opportunistic and systemic infections (A)</p> Signup and view all the answers

What distinguishes the cell walls of algae from those of prokaryotes?

<p>Rigidity and composition (C)</p> Signup and view all the answers

Which statement about fungi is correct?

<p>Fungi can spread through microscopic spores. (D)</p> Signup and view all the answers

Flashcards

Central Dogma

The flow of genetic information from DNA to RNA to protein. This is the central dogma of molecular biology.

RNA Bases

The four building blocks of RNA: adenine (A), guanine (G), cytosine (C), and uracil (U).

Evolution by Inheritance

A process of evolution where successful traits are passed down to offspring. This is the basis of natural selection.

Terraforming

The process of changing a planet's environment to be more suitable for life, often through the actions of organisms.

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Universal Tree of Life

A diagram that shows the evolutionary relationships between all living organisms.

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Evolutionary Time

The time it takes for a mutation to accumulate in DNA. Longer lines on the universal tree indicate more time has passed.

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Single-celled Ancestor

The idea that all cellular life shares a single common ancestor. It is the basis of the universal tree of life.

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Geological Timeline

The study of the origin and history of life on Earth using geological evidence.

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Diseases

Diseases that cause illness or death without physical injury or poisoning.

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Etiology

The cause or origin of a disease.

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Etiology

The study of the causes and origins of diseases.

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Sanitation

The understanding and use of sanitation practices to prevent disease, even without knowledge of microorganisms.

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Malaria from 'bad air'

The belief that swamps release harmful poison in 'bad air' that causes malaria.

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Fermentation for Preservation

The use of fermentation to preserve food, often attributed to the effects of specific locations or containers.

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Microorganisms and Disease

The discovery of microorganisms revolutionized our understanding of diseases.

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Microorganism Intent

The primary goal of microorganisms is survival, growth, and reproduction; causing disease is not their intention.

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Algae

A diverse group of eukaryotes that obtain energy through photosynthesis. They can be single-celled or multicellular and contain chlorophyll or other pigments that give them characteristic colors.

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Fungi

A diverse group of organisms that range from single-celled yeasts to multicellular filamentous molds. They secrete enzymes to break down organic materials and then absorb the released nutrients.

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Hyphae

Microscopic filaments that make up the body of filamentous fungi, like molds. They form a visible mat called a mycelium.

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Mycelium

A visible mat formed by the interwoven hyphae of filamentous fungi.

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Spores (Conidia)

Microscopic reproductive structures produced by fungi, especially filamentous molds. They are released into the environment to spread the fungus.

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Mushroom

The macroscopic reproductive structure of some fungi, often shaped like an umbrella or a cap.

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Opportunistic Fungal Infections

Infections caused by fungi that take advantage of a weakened immune system or an environment that favors their growth.

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Systemic Fungal Infections

Fungi that cause infections on the skin, in the lungs, or even throughout the body.

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Microorganisms > Humans

The impact of microorganisms on human populations can be significantly greater than the impact of human conflict.

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Influenza Pandemic v Wars

The 1918 influenza pandemic killed more Americans than all major wars combined.

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Coccus

Spherical bacteria, sometimes slightly oval or flattened on one end.

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Indirect Microbial Impact

Microorganisms can cause widespread damage without even directly infecting humans.

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Potato Famine & Microbes

The Irish Potato Famine was caused by a microbial disease that affected potatoes.

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Bacillus

Rod-shaped bacteria, with varying lengths depending on the species.

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Olive Tree Disease

A bacterial disease affecting olive trees has spread, impacting olive oil production worldwide.

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Vibrio

A short, curved rod-shaped bacteria.

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Wheat Blast

Wheat blast, a fungal disease, has destroyed wheat crops in several countries.

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Spirillum

Long, spiral-shaped bacteria, with flexible cell walls.

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Spirochete

Long, spiral-shaped bacteria with flexible cell walls and unique motility.

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Chytridiomycosis

Chytridiomycosis, a fungal disease, has severely reduced frog populations globally.

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Smallpox Eradication

Smallpox, once a devastating disease, has been eradicated through global vaccination efforts.

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Pleomorphic

Bacteria that have variable shapes, often displaying multiple forms.

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Diplococci

Pairs of bacteria that remain together after cell division.

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Streptococci

Bacteria that divide in a plane, forming long chains.

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What is a hypothesis?

A testable explanation for an observation.

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What is a control in an experiment?

A part of an experiment that remains unchanged, used as a baseline for comparison.

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What is a scientific theory?

A scientific theory is a well-substantiated explanation of some aspect of the natural world, supported by a large body of evidence.

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How does the scientific method work?

The process of science involves making observations, asking questions, forming hypotheses, conducting experiments, analyzing data, drawing conclusions, and communicating findings.

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Scientific Rules: Why are exceptions important?

Understanding the exceptions to a rule often helps understand the rule better and makes it more useful.

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In this course, how will we use rules?

In this course, we will use generalized rules to summarize material, keeping in mind that every rule has exceptions.

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Is the scientific method linear?

The process of science is cyclical. You may need to refine your hypothesis or your experiment based on your results.

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Where do observations fit into the scientific method?

Observations and questions can spark scientific inquiry, leading to the development of new hypotheses and experiments.

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Study Notes

The Scientific Method

  • Describes the process of science
  • Make an observation and ask a question
  • Develop a testable explanation (hypothesis)
  • Design experiments to test the hypothesis
    • Include a control to eliminate alternative explanations
  • Conduct the experiment, collect and analyze data
  • Draw a conclusion
  • Communicate methods, results, and conclusions
  • Scientific theory: explanation supported by significant evidence

Scientific Rules

  • Rules have exceptions
  • Understanding exceptions strengthens rule utility
  • Exceptions occur throughout the semester
  • Seek clarification of exceptions during office hours
  • Examples include the flow of information from DNA to RNA to protein, and the four RNA bases (A, G, C, and U)

Information Stored in Nucleic Acids (DNA or RNA)

  • Instruction and grading are challenging
  • Situations sometimes require educated guesses
  • Correct guesses lead to survival, reproduction, and generational memory
  • Process is slow, with many unsuccessful attempts
  • Time scale is difficult to grasp

Universal Tree of Cellular Life

  • Assesses the accumulation of mutations in DNA
  • Length of lines reflects evolutionary time
  • All cellular life evolved from a single-celled ancestor
  • Earth is approximately 4.6 billion years old
  • Life originated 3.5-3.8 billion years ago
  • Early life shaped the planet's environment, introducing oxygen.
  • Life spread and evolved to thrive in diverse habitats.

Microorganisms Affecting Humans

  • Microorganisms (MOs) affect humans in various ways
  • Their presence in the human microbiome
  • Influence on environmental processes
  • Commercial use (food, preservation, biodegradation, compounds)
  • Research tools
  • Infectious disease overview

Human Diseases and Microorganisms

  • Humans have long recognized diseases distinct from physical injury
  • Often associated with environmental factors or poisons
  • Ancient civilizations understood the significance of sanitation but not the specific causes
  • Preservation methods (fermentation), linked to conditions
  • Discoveries of microorganisms increased understanding.
  • Microorganisms, while capable of causing disease, are not inherently harmful in most cases.
  • Their evolutionary goal is survival, growth, and reproduction.
  • Understanding their needs can help manipulate their activities.

Categories of Infectious Diseases

  • Some diseases evolved to coexist with humans and our immune systems.
  • Other diseases are accidental; originating from different environments or mismatches with our biology

Infectious Disease Concepts

  • Most microorganisms are not harmful, some are beneficial
  • Pathogens cause disease through various mechanisms:
    • Direct damage
    • Toxin production
    • Waste product release
    • Immune system stimulation

Impact of Infectious Disease

  • Historical impact: examples of widespread disease like the 1918 flu and COVID-19, surpassing other global conflicts in mortality.

Microorganisms Don't Need to Infect to Cause Impact

  • Famine in Ireland (potato blight)
  • Olive tree disease impacting worldwide production
  • Wheat blast devastating crops
  • Amphibian decline caused by fungal disease

Specific Successes in Infectious Disease Management

  • Smallpox eradication
  • Reduced plague
  • Polio near-eradication
  • Measles successful containment efforts

Emerging Infectious Diseases

  • Emerging infections, often zoonotic (animal origins), are frequently observed
  • Examples of emerging diseases (such as COVID-19, Mpox, Ebola, Zika, etc.)
  • Societal factors influence the spread and impact of emerging diseases
  • Drug resistance in bacteria/microorganisms

The Different Types of Living Beings (Prokaryotic and Eukaryotic)

  • All life falls into three domains: Bacteria, Archaea, and Eukarya
  • Bacteria characteristics: single-celled prokaryotes, rigid cell walls, binary fission, wide variety of shapes and energy sources
  • Archaea characteristics: similar size and shape to bacteria, cell walls lack peptidoglycan; often extremophiles
  • Eukaryotes characteristics: single-celled or multicellular, membrane-bound organelles, diverse examples like fungi, algae, protozoa, and helminths

Acellular Infectious Agents

  • Viruses, Viroids, and Prions lack some characteristics of living cells
  • Viruses are nucleic acid (DNA or RNA) packaged in protein coats
  • Viroids consist only of RNA
  • Prions are infectious proteins, misfolded versions of normal proteins, causing normal proteins to misfold

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