Biology Taxonomy Quiz
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Questions and Answers

What is taxonomy, and how does it relate to phylogeny?

Taxonomy is the science of classifying and naming organisms, while phylogeny is the study of their evolutionary history.

What were some major limitations of the two-kingdom classification system?

The two-kingdom classification system oversimplified the diversity of life and failed to distinguish between prokaryotic and eukaryotic organisms.

What contributions did Linnaeus make to the field of taxonomy?

Linnaeus developed the binomial nomenclature system and established the taxonomic hierarchy to classify organisms.

List two advantages of the three-domain system over previous classification systems.

<p>The three-domain system better reflects the genetic differences among organisms and incorporates prokaryotic life forms into distinct domains.</p> Signup and view all the answers

Describe the characteristics distinguishing Bacteria, Archaea, and Eukarya domains.

<p>Bacteria are unicellular prokaryotes with peptidoglycan cell walls, Archaea are unicellular prokaryotes that often thrive in extreme environments, and Eukarya consists of multicellular or unicellular eukaryotes with membrane-bound organelles.</p> Signup and view all the answers

Why are scientific names used in taxonomy, and how are they formatted?

<p>Scientific names provide a universal naming system to avoid confusion, and they are formatted using binomial nomenclature, with the genus name capitalized and the species epithet italicized.</p> Signup and view all the answers

What are the major taxa in the taxonomic hierarchy?

<p>The major taxa are species, genus, family, order, class, phylum, and kingdom.</p> Signup and view all the answers

Differentiate between culture, clone, and strain in microbial taxonomy.

<p>A culture is a growth of microorganisms, a clone is a population of identical cells derived from a single cell, and a strain is a genetic variant within a species.</p> Signup and view all the answers

What is the purpose of Bergey's Manual in microbiology?

<p>Bergey's Manual serves as a comprehensive reference for the classification and identification of bacteria.</p> Signup and view all the answers

How do staining and biochemical tests aid in the identification of bacteria?

<p>Staining allows for visualization of bacterial characteristics, while biochemical tests reveal metabolic properties that differentiate species.</p> Signup and view all the answers

What defines a strain of bacteria?

<p>A strain is a group of bacterial cells derived from a single parent cell.</p> Signup and view all the answers

Which two kingdoms did Linnaeus initially divide all organisms into?

<p>Linnaeus divided organisms into two kingdoms: Plantae and Animalia.</p> Signup and view all the answers

What is the basis of Whittaker's five-kingdom classification?

<p>Whittaker's classification is based on phylogenetic hierarchy, reflecting organisms' evolution from common ancestors.</p> Signup and view all the answers

Name the three domains in Carl Woese's classification system.

<p>The three domains are Eukarya, Bacteria, and Archaea.</p> Signup and view all the answers

What types of organisms are included in the kingdom Protista?

<p>Protista includes a variety of unicellular organisms that can be autotrophic or heterotrophic.</p> Signup and view all the answers

What is Bergey’s Manual used for?

<p>Bergey’s Manual is used for the identification of prokaryotes.</p> Signup and view all the answers

List two characteristics used to classify microorganisms physically.

<p>Morphology and size of cells are two physical characteristics used for classification.</p> Signup and view all the answers

What is the role of biochemical tests in microbial classification?

<p>Biochemical tests determine enzymatic activities and the ability to utilize or produce specific chemicals.</p> Signup and view all the answers

Describe the EnteroPluri Test.

<p>The EnteroPluri Test is a rapid identification method for bacteria using one tube for 15 biochemical tests.</p> Signup and view all the answers

Identify one characteristic of Archaea that distinguishes them from Bacteria.

<p>Archaea can be extreme halophiles or hyperthermophiles, which are conditions Bacteria cannot tolerate.</p> Signup and view all the answers

What is the significance of phenylalanine in the biochemical identification of microorganisms?

<p>Phenylalanine is an amino acid tested in diagnostic procedures to determine if an organism can produce phenylalanine deaminase.</p> Signup and view all the answers

How does arabinose fermentation indicate the capability of an organism?

<p>Arabinose fermentation indicates an organism's ability to metabolize specific sugars, often reflecting its ecological niche.</p> Signup and view all the answers

What role does urease play in the identification of bacteria?

<p>Urease production tests for the ability to hydrolyze urea into ammonia, indicating certain bacterial species.</p> Signup and view all the answers

Explain the importance of the citrate test in identifying Citrobacter freundii.

<p>The citrate test assesses whether an organism can utilize citrate as its sole carbon source, which is characteristic of Citrobacter freundii.</p> Signup and view all the answers

What does the presence of H2S indicate about an organism's metabolic capabilities?

<p>The presence of H2S indicates that the organism can produce hydrogen sulfide through the reduction of sulfur-containing compounds.</p> Signup and view all the answers

How does the indole test contribute to distinguishing different types of bacteria?

<p>The indole test determines the organism's ability to convert tryptophan into indole, aiding in differentiating members of the Enterobacteriaceae family.</p> Signup and view all the answers

Why is the Voges-Proskauer (V-P) test relevant in bacterial identification?

<p>The V-P test detects acetoin production from glucose fermentation, helping distinguish between certain microorganisms.</p> Signup and view all the answers

Describe how gas production during fermentation can aid in microbial identification.

<p>Gas production indicates fermentation activity, which is essential for determining specific metabolic pathways in bacteria.</p> Signup and view all the answers

What is the significance of the code number derived from biochemical tests?

<p>The code number summarizes results from multiple biochemical tests for efficient comparison with known databases.</p> Signup and view all the answers

How can the ability to ferment lactose influence bacterial classification?

<p>Lactose fermentation helps classify bacteria, particularly in differentiating pathogenic strains from non-pathogenic ones.</p> Signup and view all the answers

What is the primary purpose of serology in microbiology?

<p>To study serum and immune responses, particularly the formation of antibodies against antigens.</p> Signup and view all the answers

Describe the principle behind the slide agglutination test.

<p>It is based on the interaction between antigen and antibody, resulting in the clumping of the antigen.</p> Signup and view all the answers

What does the G+C ratio in DNA base composition indicate?

<p>It indicates the percentage of guanine and cytosine in the DNA, helping to assess genetic relatedness between organisms.</p> Signup and view all the answers

How does PCR aid in the identification of unknown microorganisms?

<p>PCR amplifies the DNA of microorganisms that cannot be cultured, allowing for analysis and identification.</p> Signup and view all the answers

What are the key components necessary to perform an ELISA test?

<p>A labeled antibody and a sample containing the antigen to be detected are necessary.</p> Signup and view all the answers

What is the main advantage of DNA fingerprinting in microbiology?

<p>It provides detailed information about genetic similarities and differences between organisms.</p> Signup and view all the answers

Explain the role of a dichotomous key in microbiological studies.

<p>A dichotomous key guides users through a process of elimination to identify microorganisms based on specific characteristics.</p> Signup and view all the answers

What distinguishes Gram-negative rods such as Escherichia coli from Gram-positive rods like Bacillus megaterium?

<p>The key distinction is in their cell wall structures, with Gram-negative rods having a thin peptidoglycan layer and an outer membrane.</p> Signup and view all the answers

What is the importance of online databases like NCBI Genome Database in microbial classification?

<p>They provide access to genomic data that assists researchers in comparing DNA sequences and identifying microbial species.</p> Signup and view all the answers

In what scenarios might Western blotting be utilized in microbiology?

<p>It is used to confirm infections, such as HIV or Lyme disease, by identifying specific antibodies in patient serum.</p> Signup and view all the answers

Study Notes

Taxonomy and Classification

  • Taxonomy involves the classification and naming of organisms.
  • It includes systematics, which studies the evolutionary history (phylogeny) of organisms.
  • A taxon refers to a specific group of organisms, while phylogeny is the evolutionary lineage leading to that group.

Historical Contributions

  • Carl Linnaeus: Developed binomial nomenclature as a two-part naming system using Latin; first part is the genus, second part is the species epithet.
    • Examples: Escherichia coli, Vibrio cholerae.
  • Established a taxonomic hierarchy: species, genus, family, order, class, phylum, kingdom.
  • Robert Whittaker: Introduced a five-kingdom classification system, expanding upon Linnaeus's two kingdoms to include Monera, Protista, Fungi, Plantae, and Animalia.
  • Carl Woese: Proposed the three-domain system (Eukarya, Bacteria, Archaea) based on rRNA sequences.

Two-Kingdom vs. Three-Domain Systems

  • Two-kingdom system: Plantae and Animalia; limited in addressing diversity.
  • Three-domain system recognizes significant genetic differences among organisms, particularly prokaryotes.

Key Characteristics of Domains

  • Bacteria: Prokaryotic, diverse metabolic pathways.
  • Archaea: Extremophiles like methanogens, halophiles, and hyperthermophiles, distinct from bacteria.
  • Eukarya: Contains all eukaryotic organisms, including plants, animals, fungi, and protists.

Scientific Naming and Major Taxa

  • Scientific names provide precise identification, minimizing ambiguity.
  • Major taxa include species, genus, family, order, class, phylum, and kingdom.

Cultures, Clones, and Strains

  • Culture: Growth of microorganisms in a controlled environment.
  • Clone: Descendants derived from a single parent cell.
  • Strain: Genetic variant within a species, often with specific characteristics.

Characteristics of Multicellular Eukarya

  • Eukarya are differentiated based on cell structure, reproductive strategies, and modes of nutrition (e.g., plants are autotrophic; animals and fungi are heterotrophic).

Definition of Protists

  • Protists are a diverse group of mostly unicellular organisms that can be autotrophic or heterotrophic.

Distinction of Species

  • Prokaryotic species consist of cells with similar characteristics, while eukaryotic species have organisms that interbreed to produce viable offspring.

Bergy’s Manual and Identification Methods

  • Bergey’s Manual: A comprehensive resource aiding in prokaryotic identification.
  • Identification techniques include morphology, biochemical tests, serological analysis, and nucleic acid testing.

Identification Techniques

  • Biochemical Tests: Assess enzymatic activities to identify metabolic capabilities (e.g., fermentation).
  • Serological Tests: Evaluate antigen-antibody reactions to identify bacteria.
    • ELISA and Western blotting are common methods.
  • Nucleic Acid Analysis:
    • DNA base composition compares guanine-cytosine ratios.
    • DNA fingerprinting analyzes restriction enzyme digest patterns.
    • PCR amplifies unknown microbial DNA.

Dichotomous Keys

  • Dichotomous keys guide users through a systematic process to identify organisms based on specific characteristics.
  • Useful for differentiating between species using a step-by-step flowchart approach.

Practical Application

  • Constructing dichotomous keys and utilizing rapid identification tests like the EnteroPluri Test for bacteria demonstrate applied taxonomy in microbiological studies.

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Description

Test your understanding of taxonomy, the classification and naming of organisms. This quiz covers key concepts such as the limitations of the two-kingdom system, contributions of major scientists, and characteristics of the three domains of life: Bacteria, Archaea, and Eukarya.

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