Biology Required Practical 9: Investigating Respiration Rate of Single-Celled Organisms

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Explain why the fluid in the capillary tube moves down a pressure gradient in a respirometer measuring anaerobic respiration.

The fluid moves down the pressure gradient because as yeast anaerobically respire and release CO2, the volume of gas and pressure in the container increase.

What measurements are needed to calculate the rate of respiration in a respirometer?

Distance moved by liquid, time taken, radius of capillary tube, mass of organisms.

Why is it important to allow time for oxygen to be used or respired before conducting experiments on anaerobic respiration?

Allowing time for oxygen to be used ensures that the organisms have consumed the available oxygen and switched to anaerobic respiration.

Explain why the respirometer apparatus is left open for 10 minutes before conducting experiments.

The apparatus is left open to allow for equilibration and to compensate for any pressure expansion or changes.

Learn about the importance of using an airtight apparatus to measure the respiration rate of organisms accurately. This quiz covers Method 2 of Required Practical 9 and discusses the use of redox indicator dyes like Methylene blue for measuring the rate of respiration.

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