Biology: Prokaryotes, Eukaryotes, and the Three Domains

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Questions and Answers

What is the basis of genotypic classification of bacteria?

  • Cultural characteristics and environmental factors
  • DNA-DNA hybridization and G+C content (correct)
  • Antigenic structure and nutrition
  • Morphological characteristics

What is the main purpose of 16S rRNA sequences in classifying prokaryotes?

  • To identify the shape of the bacterium
  • To analyze the nucleotide sequence differences (correct)
  • To detect the presence of peptidoglycan in the cell wall
  • To determine the G+C content of the DNA

Which of the following is a characteristic of Gram-positive bacteria?

  • They have a thick peptidoglycan layer (correct)
  • They are halophiles
  • They lack a cell wall
  • They have a thin peptidoglycan layer

What is the primary function of genetic probes in DNA analysis?

<p>To identify specific DNA sequences (D)</p> Signup and view all the answers

Which group of bacteria includes methanogens and extreme thermophiles?

<p>Archaea (D)</p> Signup and view all the answers

What is the primary basis of phenotypic classification of bacteria?

<p>All of the above (D)</p> Signup and view all the answers

What is the main advantage of using rRNA sequence analysis in classifying prokaryotes?

<p>It provides evolutionary context and makes biological sense (B)</p> Signup and view all the answers

What is the primary characteristic of halophiles?

<p>They thrive in high-salt environments (A)</p> Signup and view all the answers

What is a characteristic of Archaea that distinguishes them from bacteria?

<p>Having unique genetic sequences in their rRNA (C)</p> Signup and view all the answers

Which of the following is NOT a characteristic of Eukaryotes?

<p>Lacking membrane lipids (B)</p> Signup and view all the answers

What is the name of the kingdom that includes protozoa in the Whittaker classification?

<p>Kingdom Protista (A)</p> Signup and view all the answers

What is a characteristic of protozoa?

<p>Lacking photosynthetic capability (D)</p> Signup and view all the answers

Which of the following is an example of an extreme environment that Archaea can thrive in?

<p>High-temperature hot springs (B)</p> Signup and view all the answers

What is the term for organisms that are adapted to live in extreme environments?

<p>Extremophiles (B)</p> Signup and view all the answers

What is the key characteristic of heterotrophic organisms like fungi?

<p>Cannot produce their own food (D)</p> Signup and view all the answers

What is the primary source of energy and carbon for fungi?

<p>Organic compounds (A)</p> Signup and view all the answers

What is the common characteristic of the cells of fungi?

<p>Usually organized into branched, multinucleate filaments (A)</p> Signup and view all the answers

What is the term for the network of tubes that make up mushrooms?

<p>Hypae (C)</p> Signup and view all the answers

How many species of fungi are estimated to exist?

<p>100,000 (C)</p> Signup and view all the answers

What is the characteristic of yeast fungi?

<p>Unicellular (B)</p> Signup and view all the answers

What is the primary basis for fungal classification?

<p>Spore-forming structures and sexual spores (D)</p> Signup and view all the answers

What is the term for the filaments that make up mycelia?

<p>Hyphae (C)</p> Signup and view all the answers

What is the primary method used to quantify viruses?

<p>Counting the number of plaques (B)</p> Signup and view all the answers

What is the study of viruses called?

<p>Virology (D)</p> Signup and view all the answers

What are the two main components of a virus?

<p>Nucleic acid and capsid (C)</p> Signup and view all the answers

What is unique about retroviruses?

<p>They have a single-stranded RNA genome (B)</p> Signup and view all the answers

What is the primary function of the capsid?

<p>To protect the viral genome (D)</p> Signup and view all the answers

What is the classification of viruses based on?

<p>Multiple parameters including the type of genomic nucleic acid, size of the virion and genome, and more (A)</p> Signup and view all the answers

What is a characteristic of viroids?

<p>They are infectious pieces of naked RNA (D)</p> Signup and view all the answers

What is a characteristic of prions?

<p>They are infectious proteins (C)</p> Signup and view all the answers

What is the name of the domain that includes humans, animals, and plants?

<p>Eukarya (C)</p> Signup and view all the answers

What does the prefix 'pro' mean in the term 'prokaryotes'?

<p>Before (D)</p> Signup and view all the answers

What is the level of classification that is used to identify each creature in binomial nomenclature?

<p>Species (B)</p> Signup and view all the answers

What is the term for the naming system used to identify microorganisms?

<p>Binomial Nomenclature (D)</p> Signup and view all the answers

What is the correct way to write the genus name in binomial nomenclature?

<p>In uppercase letters (D)</p> Signup and view all the answers

What is the characteristic that distinguishes eukaryotes from prokaryotes?

<p>Presence of a nucleus (D)</p> Signup and view all the answers

What is the order of classification in the taxonomic hierarchy?

<p>Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species (D)</p> Signup and view all the answers

What is the abbreviation for the species Escherichia coli?

<p>E.coli (C)</p> Signup and view all the answers

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Study Notes

Cell Classification

  • Three domains: Bacteria, Archaea, and Eukarya
  • Bacteria: prokaryotes, lack peptidoglycan, binary fission, energy from organic chemicals, inorganic chemicals, or photosynthesis
  • Archaea: prokaryotes, lack peptidoglycan, live in extreme environments, include methanogens, extreme halophiles, and extreme thermophiles
  • Eukarya: eukaryotic cells, have a nucleus, organelles, reproduce asexually or sexually, examples include protozoans, molds, plants, and animals

Taxonomic Hierarchy

  • Domain: highest level of classification
  • Kingdom: second level of classification
  • Phylum: third level of classification
  • Class: fourth level of classification
  • Order: fifth level of classification
  • Family: sixth level of classification
  • Genus: seventh level of classification
  • Species: eighth level of classification

Binomial Nomenclature

  • Uses Genus and Species names to identify each creature
  • Examples: Escherichia coli, Staphylococcus aureus, Bacillus subtilis

Microorganism Classification

  • Phenotypic classification: based on shape, size, and staining
  • Genotypic classification: based on DNA-DNA hybridization, G+C content, and rRNA sequence

Bacteria Shapes

  • Three main shapes: Cocci, Bacilli, and Spiral

Cell Wall Composition

  • Four groups: Gram-positive, Gram-negative, bacteria without cell walls, and bacteria with chemically unique cell walls

rRNA Sequence

  • Used to classify prokaryotes
  • 16S rRNA sequences and 23S rRNA sequences are used

Archaea

  • Prokaryotes, lack peptidoglycan, live in extreme environments
  • Include methanogens, extreme halophiles, and extreme thermophiles

Eukaryotes

  • Eukaryotic cells, have a nucleus, organelles, reproduce asexually or sexually
  • Examples include protozoans, molds, plants, and animals

Kingdom Protista

  • Microscopic unicellular organisms, lack photosynthetic capability, usually motile, reproduce by asexual fission
  • Examples include protozoans

Kingdom Fungi

  • Heterotrophic, require organic compounds for energy and carbon source
  • Cells are usually organized into branched, multinucleate filaments which absorb digested food from the external environment
  • Examples include yeasts, molds, and mushrooms

Fungal Characteristics

  • Chitin cell walls, use organic chemicals for energy, multicellular consisting of masses of mycelia, which are composed of filaments called hyphae
  • Yeasts are unicellular

Viruses

  • Acellular, obligate intracellular parasites, no ribosomes or means of protein synthesis, no ATP generating system
  • Not alive
  • Classified based on type of genomic nucleic acid, size of virion and genome, capsid structure, host, and replication mechanism

Virus Structure

  • Two parts: nucleic acid (either DNA or RNA) and capsid (protein coat)
  • Some capsids surrounded by envelopes

Viroids and Prions

  • Viroids: infectious pieces of naked RNA, cause plant diseases, 300-400 nucleotides long, closed, folded 3D shape
  • Prions: infectious proteins, cause diseases such as Mad cow and Creutzfeldt-Jakob

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