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Biology Lab Quiz: Gel Electrophoresis

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Questions and Answers

How can the resolution of bands in gel electrophoresis be improved?

By increasing the voltage (correct)

By using a different type of gel (correct)

By comparing with known standards

By using a different type of buffer

What is the purpose of using a comb during the preparation of a gel for electrophoresis?

To polymerize the gel

To remove bubbles from the gel

To create wells for loading samples (correct)

To mix the gel solution

Which of the following is a common use of gel electrophoresis in medical diagnostics?

Gene cloning

Detecting genetic mutations (correct)

DNA sequencing

Protein purification

Which of the following is an example of adsorption chromatography?

Chromatography using a column containing silica gel or alumina Signup and view all the answers

What are the two main components required for all chromatographic methods?

Stationary phase and mobile phase Signup and view all the answers

Which of the following best describes the function of a spectrophotometer?

To determine the transmittance and absorbance of a solution Signup and view all the answers

Study Notes

Gel Electrophoresis and Chromatography

Gel Electrophoresis

The purpose of using a comb during gel preparation is to create wells for loading samples.
Gel electrophoresis is used in medical diagnostics to detect genetic mutations.
The technique is not used for DNA sequencing, gene cloning, or protein purification.

Chromatography

Adsorption chromatography is an example of chromatography using a column containing silica gel or alumina.
There are two main components required for all chromatographic methods: stationary phase and mobile phase.
Other components, such as adsorption and partition, eluent and eluate, and ion exchange and molecular exclusion, are not the primary components required for chromatography.