Questions and Answers
How can the resolution of bands in gel electrophoresis be improved?
What is the purpose of using a comb during the preparation of a gel for electrophoresis?
Which of the following is a common use of gel electrophoresis in medical diagnostics?
Which of the following is an example of adsorption chromatography?
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What are the two main components required for all chromatographic methods?
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Which of the following best describes the function of a spectrophotometer?
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Study Notes
Gel Electrophoresis and Chromatography
Gel Electrophoresis
- The purpose of using a comb during gel preparation is to create wells for loading samples.
- Gel electrophoresis is used in medical diagnostics to detect genetic mutations.
- The technique is not used for DNA sequencing, gene cloning, or protein purification.
Chromatography
- Adsorption chromatography is an example of chromatography using a column containing silica gel or alumina.
- There are two main components required for all chromatographic methods: stationary phase and mobile phase.
- Other components, such as adsorption and partition, eluent and eluate, and ion exchange and molecular exclusion, are not the primary components required for chromatography.
Spectrophotometry
- Key terms related to spectrophotometry include: absorbance, analytical, composition, concentration, reactions, spectrophotometer, and transmittance.
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Description
Multiple choice questions about gel electrophoresis, its preparation, and its applications in medical science.