Biology Lab Quiz: Gel Electrophoresis
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Questions and Answers

How can the resolution of bands in gel electrophoresis be improved?

  • By increasing the voltage (correct)
  • By using a different type of gel (correct)
  • By comparing with known standards
  • By using a different type of buffer

What is the purpose of using a comb during the preparation of a gel for electrophoresis?

  • To polymerize the gel
  • To remove bubbles from the gel
  • To create wells for loading samples (correct)
  • To mix the gel solution

Which of the following is a common use of gel electrophoresis in medical diagnostics?

  • Gene cloning
  • Detecting genetic mutations (correct)
  • DNA sequencing
  • Protein purification

Which of the following is an example of adsorption chromatography?

<p>Chromatography using a column containing silica gel or alumina (B)</p> Signup and view all the answers

What are the two main components required for all chromatographic methods?

<p>Stationary phase and mobile phase (B)</p> Signup and view all the answers

Which of the following best describes the function of a spectrophotometer?

<p>To determine the transmittance and absorbance of a solution (B)</p> Signup and view all the answers

Study Notes

Gel Electrophoresis and Chromatography

Gel Electrophoresis

  • The purpose of using a comb during gel preparation is to create wells for loading samples.
  • Gel electrophoresis is used in medical diagnostics to detect genetic mutations.
  • The technique is not used for DNA sequencing, gene cloning, or protein purification.

Chromatography

  • Adsorption chromatography is an example of chromatography using a column containing silica gel or alumina.
  • There are two main components required for all chromatographic methods: stationary phase and mobile phase.
  • Other components, such as adsorption and partition, eluent and eluate, and ion exchange and molecular exclusion, are not the primary components required for chromatography.

Spectrophotometry

  • Key terms related to spectrophotometry include: absorbance, analytical, composition, concentration, reactions, spectrophotometer, and transmittance.

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Multiple choice questions about gel electrophoresis, its preparation, and its applications in medical science.

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