Podcast
Questions and Answers
What is the term for the fusion of the sperm and egg nuclei?
What is the term for the fusion of the sperm and egg nuclei?
What is the normal diploid number of chromosomes found in a zygote?
What is the normal diploid number of chromosomes found in a zygote?
What is the primary role of progesterone in the early stages of pregnancy?
What is the primary role of progesterone in the early stages of pregnancy?
What occurs during implantation that may be mistaken for menstruation?
What occurs during implantation that may be mistaken for menstruation?
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What occurs if the morula arrives in the uterus prematurely?
What occurs if the morula arrives in the uterus prematurely?
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What is the solid ball of 16 cells formed by the rapidly dividing zygote called?
What is the solid ball of 16 cells formed by the rapidly dividing zygote called?
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What is the primary role of the trophoblast after implantation?
What is the primary role of the trophoblast after implantation?
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Which part of the blastocyst is responsible for inserting into the endometrium during implantation?
Which part of the blastocyst is responsible for inserting into the endometrium during implantation?
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What does the inner cell mass of the blastocyst become?
What does the inner cell mass of the blastocyst become?
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What happens to the zona pellucida during the implantation process?
What happens to the zona pellucida during the implantation process?
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What phase follows the formation of the morula during early stages of pregnancy?
What phase follows the formation of the morula during early stages of pregnancy?
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A triploid cell results from what event during fertilization?
A triploid cell results from what event during fertilization?
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Approximately how many cells does a blastocyst consist of when ready for implantation?
Approximately how many cells does a blastocyst consist of when ready for implantation?
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What is the normal duration of pregnancy counted from?
What is the normal duration of pregnancy counted from?
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What is the role of the zona pellucida during the early stages of pregnancy?
What is the role of the zona pellucida during the early stages of pregnancy?
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What major change occurs in the endometrial tissue at the contact site during implantation?
What major change occurs in the endometrial tissue at the contact site during implantation?
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During which trimester is the highest rate of miscarriage observed?
During which trimester is the highest rate of miscarriage observed?
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What is the definition of preterm birth?
What is the definition of preterm birth?
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What event marks Day 6 in the early stages of pregnancy?
What event marks Day 6 in the early stages of pregnancy?
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What processes begin with spermatozoa penetrating into the egg's corona radiata?
What processes begin with spermatozoa penetrating into the egg's corona radiata?
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Which hormone is not produced by the gonads?
Which hormone is not produced by the gonads?
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What is the correct sequence of events in fertilization?
What is the correct sequence of events in fertilization?
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What term is used for a live birth after 42 weeks of pregnancy?
What term is used for a live birth after 42 weeks of pregnancy?
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Which of the following is a function of the gonads?
Which of the following is a function of the gonads?
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What characterizes the placenta's hormone secretion?
What characterizes the placenta's hormone secretion?
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Which hormone is produced first by the developing chorion?
Which hormone is produced first by the developing chorion?
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What is a primary function of hCG during early pregnancy?
What is a primary function of hCG during early pregnancy?
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Which hormone secreted by the placenta is crucial for masculinizing the fetal reproductive tract?
Which hormone secreted by the placenta is crucial for masculinizing the fetal reproductive tract?
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What role does hPL (human placental lactogen) play during pregnancy?
What role does hPL (human placental lactogen) play during pregnancy?
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Which of the following hormones is NOT primarily secreted by the placenta?
Which of the following hormones is NOT primarily secreted by the placenta?
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Which statement about the placenta is true?
Which statement about the placenta is true?
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What is the effect of oxytocin during pregnancy?
What is the effect of oxytocin during pregnancy?
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What is the primary function of progesterone in the uterus?
What is the primary function of progesterone in the uterus?
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Which hormone significantly increases uterine responsiveness to oxytocin during pregnancy?
Which hormone significantly increases uterine responsiveness to oxytocin during pregnancy?
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What is the primary source of estrogen during pregnancy?
What is the primary source of estrogen during pregnancy?
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Which of the following factors contributes to cervical ripening and increased uterine responsiveness to oxytocin?
Which of the following factors contributes to cervical ripening and increased uterine responsiveness to oxytocin?
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How does oxytocin contribute to labor initiation?
How does oxytocin contribute to labor initiation?
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Which of the following is NOT a function of oxytocin?
Which of the following is NOT a function of oxytocin?
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What is the typical duration of the placental delivery stage after childbirth?
What is the typical duration of the placental delivery stage after childbirth?
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What is the approximate weight of a mature placenta at term?
What is the approximate weight of a mature placenta at term?
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What happens to the blastocyst after it burrows into the decidua?
What happens to the blastocyst after it burrows into the decidua?
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Which of the following structures primarily contributes to the formation of the placenta?
Which of the following structures primarily contributes to the formation of the placenta?
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What is the function of the trophoblastic layer during the early stages of pregnancy?
What is the function of the trophoblastic layer during the early stages of pregnancy?
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How is uteroplacental circulation established?
How is uteroplacental circulation established?
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What is the role of chorionic villi in the placenta?
What is the role of chorionic villi in the placenta?
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Which process occurs on day 12 of embryonic development in relation to the decidua?
Which process occurs on day 12 of embryonic development in relation to the decidua?
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What is the significance of the thin barrier between maternal and fetal blood in the placenta?
What is the significance of the thin barrier between maternal and fetal blood in the placenta?
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What are cotyledons in the context of the placenta?
What are cotyledons in the context of the placenta?
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Which of the following events directly precedes the formation of the morula?
Which of the following events directly precedes the formation of the morula?
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The blastocyst's primary function is to:
The blastocyst's primary function is to:
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What is the primary role of the trophoblast during implantation?
What is the primary role of the trophoblast during implantation?
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The endometrial secretory phase is characterized by:
The endometrial secretory phase is characterized by:
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Which statement accurately describes the relationship between the morula and the blastocyst?
Which statement accurately describes the relationship between the morula and the blastocyst?
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Why is it crucial for sufficient progesterone to be present for the morula to enter the uterus?
Why is it crucial for sufficient progesterone to be present for the morula to enter the uterus?
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What would be the most likely consequence of a premature arrival of the morula in the uterine cavity?
What would be the most likely consequence of a premature arrival of the morula in the uterine cavity?
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What is the main reason for the triploid cell being lethal?
What is the main reason for the triploid cell being lethal?
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Which hormone is most closely associated with signaling that ovulation has occurred?
Which hormone is most closely associated with signaling that ovulation has occurred?
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What primary role does fetal cortisol play during the parturition process?
What primary role does fetal cortisol play during the parturition process?
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During labor, which hormone's increase is crucial for uterine changes that lead to parturition?
During labor, which hormone's increase is crucial for uterine changes that lead to parturition?
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Which of these hormones is indicative of preparing the uterine environment for pregnancy?
Which of these hormones is indicative of preparing the uterine environment for pregnancy?
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What is the primary function of GATA-4 in embryogenesis?
What is the primary function of GATA-4 in embryogenesis?
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What is the primary estrogen synthesized by the placenta from fetal DHEA?
What is the primary estrogen synthesized by the placenta from fetal DHEA?
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What is the primary function of the placental barrier?
What is the primary function of the placental barrier?
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During which stage of labor does cervical dilation primarily occur?
During which stage of labor does cervical dilation primarily occur?
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What significant change in progesterone levels is observed during the last 7 months of gestation?
What significant change in progesterone levels is observed during the last 7 months of gestation?
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Which blood vessels are contained within the umbilical cord?
Which blood vessels are contained within the umbilical cord?
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What is the duration range of Stage 2 of labor during a typical first delivery?
What is the duration range of Stage 2 of labor during a typical first delivery?
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How does the amniotic sac contribute to fetal development?
How does the amniotic sac contribute to fetal development?
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What does the placenta extract from fetal blood for conversion into estrogen?
What does the placenta extract from fetal blood for conversion into estrogen?
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What is the length of a typical umbilical cord at full term?
What is the length of a typical umbilical cord at full term?
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What is the frequency of uterine contractions shortly before the transition from Stage 1 to Stage 2 of labor?
What is the frequency of uterine contractions shortly before the transition from Stage 1 to Stage 2 of labor?
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What happens to the umbilical cord immediately after childbirth?
What happens to the umbilical cord immediately after childbirth?
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What process is critically related to the timing of implantation?
What process is critically related to the timing of implantation?
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What is the role of uterine contractions during the second stage of labor?
What is the role of uterine contractions during the second stage of labor?
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What is the composition of the umbilical vein in the umbilical cord?
What is the composition of the umbilical vein in the umbilical cord?
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Which structure primarily forms the fetal part of the placenta?
Which structure primarily forms the fetal part of the placenta?
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What structure is primarily responsible for the invasive behavior during the establishment of uteroplacental circulation?
What structure is primarily responsible for the invasive behavior during the establishment of uteroplacental circulation?
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What role does the chorion play after the embedding of the embryo into the decidua?
What role does the chorion play after the embedding of the embryo into the decidua?
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What physical change occurs to the trophoblastic layer by day 12 of embryonic development?
What physical change occurs to the trophoblastic layer by day 12 of embryonic development?
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What is the significance of the interlocking maternal and fetal structures in the placenta?
What is the significance of the interlocking maternal and fetal structures in the placenta?
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What happens to maternal blood as it interacts with the developing structures of the placenta?
What happens to maternal blood as it interacts with the developing structures of the placenta?
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Which characteristic makes the placenta unique among endocrine tissues?
Which characteristic makes the placenta unique among endocrine tissues?
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What is the main form of nutrient supply to the embryo during the initial stages before the placenta fully develops?
What is the main form of nutrient supply to the embryo during the initial stages before the placenta fully develops?
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What is the main structural feature that defines cotyledons in the placenta?
What is the main structural feature that defines cotyledons in the placenta?
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What stimulates the corpus luteum to remain functional during early pregnancy?
What stimulates the corpus luteum to remain functional during early pregnancy?
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Which statement accurately describes the barrier between maternal and fetal blood in the placenta?
Which statement accurately describes the barrier between maternal and fetal blood in the placenta?
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Which hormone is responsible for masculinizing the developing reproductive tract in male fetuses?
Which hormone is responsible for masculinizing the developing reproductive tract in male fetuses?
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What is the primary factor influencing the rate of hormone secretion by the placenta?
What is the primary factor influencing the rate of hormone secretion by the placenta?
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What dual role does the placenta fulfill during pregnancy?
What dual role does the placenta fulfill during pregnancy?
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Which of the following hormones increases progesterone production during pregnancy?
Which of the following hormones increases progesterone production during pregnancy?
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What is one of the first endocrine events in pregnancy?
What is one of the first endocrine events in pregnancy?
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What prevents the corpus luteum from degenerating during early pregnancy?
What prevents the corpus luteum from degenerating during early pregnancy?
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Flashcards
Fertilization
Fertilization
The process where sperm penetrates the egg, leading to conception.
Implantation
Implantation
When a fertilized egg attaches to the uterine wall.
Duration of Pregnancy
Duration of Pregnancy
Normal pregnancy lasts 40 weeks from the last menstrual period.
Trimesters of Pregnancy
Trimesters of Pregnancy
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Miscarriage
Miscarriage
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Gonads
Gonads
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Endocrine Function of Placenta
Endocrine Function of Placenta
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Parturition
Parturition
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Blastocyst embedding
Blastocyst embedding
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Trophoblastic digestion
Trophoblastic digestion
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Endocrine Function of the Placenta
Endocrine Function of the Placenta
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Uteroplacental circulation
Uteroplacental circulation
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Transient Tissue
Transient Tissue
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Chorion
Chorion
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Hormone Secretion Control
Hormone Secretion Control
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Chorionic villi
Chorionic villi
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Key Placental Hormones
Key Placental Hormones
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Cotyledons
Cotyledons
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Human Chorionic Gonadotropin (hCG)
Human Chorionic Gonadotropin (hCG)
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Placental barrier
Placental barrier
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Function of hCG
Function of hCG
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Placenta composition
Placenta composition
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Leydig Cells Stimulation
Leydig Cells Stimulation
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Other Important Pregnancy Hormones
Other Important Pregnancy Hormones
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Acrosome Reaction
Acrosome Reaction
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Zygote
Zygote
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Morula
Morula
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Blastocyst
Blastocyst
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Polyspermy
Polyspermy
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Progesterone
Progesterone
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Zona Pellucida
Zona Pellucida
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Trophoblast
Trophoblast
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Implantation Bleeding
Implantation Bleeding
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Capacitation
Capacitation
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Endometrium
Endometrium
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Decidua
Decidua
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Stage 3 Delivery
Stage 3 Delivery
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Uterine Contractions
Uterine Contractions
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Effacement
Effacement
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Estrogen
Estrogen
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Oxytocin
Oxytocin
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Cervical Ripening
Cervical Ripening
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Onset of Parturition
Onset of Parturition
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Zygote Formation
Zygote Formation
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Meiotic Division
Meiotic Division
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Morula Development
Morula Development
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Blastocyst Structure
Blastocyst Structure
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Progesterone Role
Progesterone Role
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Polyspermy Consequence
Polyspermy Consequence
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Blastocyst Implantation
Blastocyst Implantation
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CRH level in labor
CRH level in labor
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Luteinizing hormone
Luteinizing hormone
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GATA-4
GATA-4
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Role of cortisol in labor
Role of cortisol in labor
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Estrogen in pregnancy
Estrogen in pregnancy
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Thyroid Hormones Role in Pregnancy
Thyroid Hormones Role in Pregnancy
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Syncytiotrophoblasts
Syncytiotrophoblasts
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Endovascular trophoblast invasion
Endovascular trophoblast invasion
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Histotrophic nutrition
Histotrophic nutrition
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Myometrium Growth
Myometrium Growth
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Uterine Blood Flow
Uterine Blood Flow
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Uterine Receptors
Uterine Receptors
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Estrogen Increase
Estrogen Increase
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Stages of Labor
Stages of Labor
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Cervical Dilation
Cervical Dilation
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Delivery Time
Delivery Time
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Formation of Estriol
Formation of Estriol
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Placenta
Placenta
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Umbilical Cord
Umbilical Cord
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Amniotic Sac
Amniotic Sac
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Umbilical Vein
Umbilical Vein
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Umbilical Arteries
Umbilical Arteries
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Amniotic Fluid
Amniotic Fluid
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Fetal Membranes
Fetal Membranes
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Study Notes
Pregnancy Course Information
- Course: Endocrine and Breast
- Lecturer: Brona Murphy - [email protected]
- Lecturer: Ebrahim Rajab - [email protected]
Learning Outcomes
- Outline the processes of fertilization & implantation
- Describe the endocrine function of the placenta and the hormones involved
- Explain the endocrine factors that trigger parturition and maintain labor
Recap from Previous Lectures - The Gonads
- The primary reproductive organs (gonads) consist of the testes in males and the ovaries in females
- Mature gonads produce gametes (sperm and ova) and secrete sex hormones (testosterone, progesterone, and estrogen)
- Gonads and reproductive system do not contribute to homeostasis but are essential for the perpetuation of the species
Recap from Previous Lectures - Correlation between hormonal levels & cyclic ovarian & uterine changes
- FSH and LH stimulate oocyte development and follicle growth
- LH burst triggers ovulation
- LH stimulates development and maintenance of corpus luteum
- Plasma concentrations of estrogen and progesterone fluctuate throughout cycle phases
Pregnancy
- Pregnancy begins with fertilization of the ovum and subsequent implantation into the uterine wall
- Pregnancy duration is measured in weeks of gestation from the first day of the last menstrual period, typically 40 weeks (280 days)
- Preterm birth: live birth before 37 weeks
- Post-term birth: live birth after 42 weeks
- Trimesters of pregnancy: First (weeks 1-13), Highest rate of miscarriage; Second (weeks 14-26); Third (weeks 27-40)
Fertilization/Conception
- Fertilization is a series of processes starting when sperm penetrate the corona radiata surrounding the egg, culminating in the intermingling of maternal and paternal chromosomes
- Oviduct (specifically, the ampulla) is the usual site of fertilization, ideally within 24 hours post-ovulation
- Sperm can survive for up to 5 days in the female reproductive tract
- Sperm cells undergo functional maturation (capacitation) in the female genital tract, allowing them to penetrate the ovulated oocyte (impregnation)
Fertilization/Conception - Additional details
- When released, the oocyte is surrounded by the corona radiata (follicular cells) and zona pellucida (glycoprotein matrix)
- Human fertilization begins when sperm penetrates the corona radiata and binds to a sugar group in the zona pellucida
Capacitation
- Spermatozoa recovered at the time of ejaculation are unable to fertilize for several hours
- After intercourse, sperm recovered from the uterus or Fallopian tube are capable of immediate fertilization due to capacitation
- Capacitation results in the removal of a glycoprotein coat from the sperm cell membrane surface by enzymes present in the uterus
- Changes in the sperm's acrosomal cap allow acrosome reaction
- Removal of cholesterol, glycoproteins, and proteins from plasma membrane around the sperm head
- Hyperactivity of the sperm tail
Acrosome Reaction
- The fertilizing sperm penetrates the corona radiata and binds to ZP3 receptors in the zona pellucida, triggering the acrosome reaction
- Hydrolytic enzymes in the acrosome are released onto the zona pellucida, creating a pathway for sperm to reach the ovum's plasma membrane
- Fusion of sperm and ovum plasma membranes
- The sperm nucleus enters the ovum cytoplasm, activating the ovum and initiating fertilization
Early Stages of Pregnancy
- Within 1 hour, sperm and egg nuclei fuse to form a zygote.
- Zygote completes its 2nd meiotic division to develop into normal diploid number of chromosomes (46 in humans)
- Within 3-4 days, zygote develops into a morula (a solid ball of 16 cells)
- Rising progesterone levels from the developing corpus luteum stimulate glycogen release from the endometrium for embryonic energy
- The morula matures into a blastocyst, accumulating nutrients in the uterine lumen
- The blastocyst implants in the uterine wall, if prematurely arrives, it dies.
Early Stages of Pregnancy - Implantation
- The blastocyst implants into the uterine lining
- Trophoblast cells penetrate endometrium, forming the placenta later on
- Implantation occurs after the zona pellucida dissolves
- The inner cell mass differentiates into the embryo
- Implantation bleeding may occur, potentially mistaken for menstruation
Summary of Early Stages of Pregnancy
- Day 0: Capacitation, fertilization, impregnation, single-celled zygote
- Day 1-5: Zygote travels down fallopian tube, divisions into morula, and then into blastocyst
- Day 6: Zona pellucida dissolves; implantation
Implantation
- Endometrium is suited to implantation approximately one week after ovulation
- Inner cell mass becomes the embryo
- The trophoblast performs dual functions in supporting the embryo and developing the fetal portion of the placenta
Implantation (Further Detail)
- Trophoblastic cells release enzymes, guiding them through the endometrial lining
- Plasma membranes of advancing trophoblastic cells degenerate, forming the fetal portion of the placenta
- Endometrial tissue near contact site dramatically changes to support the embryo
- Blastocyst burrows into the decidua, with its surface completely covered by endometrial cells.
- Trophoblast layer digests surrounding cells to supply energy to the embryo until placenta develops
Placenta
- Uteroplacental circulation is established with multiple steps—endometrium and vascular remodeling
- Embryo implants in the decidua by day 12 of the pregnancy, and is surrounded by chorionic villi
- Chorionic villi form from trophoblast cells and reach into the pools of maternal blood, forming cotyledons.
- Maternal blood leaks into these cavities
Placenta (Further Detail)
- Maternal and fetal blood do not mix but exchange nutrients across a thin barrier
- Entire system of maternal (decidual) and fetal (chorionic) structures compose the placenta
- Placenta develops from trophoblastic and decidual tissue
Endocrine Function of the Placenta
- The placenta acts as a unique endocrine tissue, connecting embryo and mother
- Hormones secreted are not subject to external control, but levels depend on pregnancy stage
- The placenta secretes steroid and peptide hormones, adapting the maternal body to pregnancy
hCG (Human Chorionic Gonadotropin)
- Early event in pregnancy: secreted by the developing chorion (later placenta)
- Stimulates and maintains corpus luteum to prevent menstruation, providing progesterone until placenta takes over
- Increases progesterone production by the trophoblast
- In male fetuses, it stimulates Leydig cells for testosterone production, masculinizng reproductive tract.
hPL (Human Placental Lactogen)
- Initially produced by trophoblast, then placenta
- Lactogenic & growth hormone-like actions — stimulates mammary glands.
- Less potent than prolactin and GH for milk production and growth processes
- Ensures adequate fuel for fetus (carbohydrates, proteins, and fats), aiding in adaptation to pregnancy
- Antagonistic action to insulin to support pregnancy-related glucose homeostasis
Progesterone
- Initially secreted by corpus luteum, then placenta
- Essential for maintaining the uterus and early embryo, facilitating implantation
- Inhibits contractions to prevent miscarriage
- Important for fetal development and supporting maternal immunological system
- Serves as a precursor to steroid production in adrenal glands, stimulating mammary development.
Estrogens
- Primarily secreted by the placenta
- Increase uterine size, blood flow, and uterine receptor production for progesterone and oxytocin.
- Essential for uterine development and fetal organ maturation
- Contributes substantially to mammary duct growth
Secretion of Estrogens and Progesterone by the Placenta
- Placenta converts fetal DHEA into estrogen and extracts DHEA from fetal blood
- Placenta produces estrogen only after the fetal adrenal cortex is secreting DHEA (the direct source of fetal DHEA)
- The main estrogen product of the placenta is estriol
- Measurement of estriol in maternal urine can assess fetal viability
Stages of Labour
- Stage 1 (Cervical dilation & effacement): Cervical dilation and softening, rhythmic contractions
- Stage 2 (Delivery of baby): Begins with complete cervical dilation; intense coordinated contractions expel the fetus
- Stage 3 (Delivery of placenta): Uterine contractions separate the placenta from the uterine wall
Factors That Trigger Onset of Parturition
- Hormones are progesterone, estrogen, prostaglandins, oxytocin, and CRH
- Mechanical factors are distention of uterine muscles and softening of the cervix
Factors That Trigger Onset of Parturition (Oxytocin)
- Oxytocin is produced by the maternal hypothalamus and increases throughout pregnancy
- Uterine responsiveness to oxytocin is much higher during labor than in non-pregnant women
- Wide acceptance that labor begins when myometrial responsiveness to oxytocin is critical to induce strong and coordinated contractions
- Clinically useful for inducing labor
- Plays a key role in bonding between mother and child
Factors That Trigger Onset of Parturition (Ferguson Reflex)
- Uterine contractions drive the fetus towards the cervix
- Head pushing against the cervix opens the cervical canal, stimulating oxytocin release
- Oxytocin further stimulates uterine contractions as a positive feedback loop
- Oxytocin causes prostaglandin production and further intensifies uterine contractions.
Factors That Raise Levels of Placental Estrogen Secretion
- Corticotrophin-releasing hormone (CRH), secreted by the fetal placenta, regulates fetal cortisol and DHEA output by adrenal cortex
- Increased CRH leads to increased fetal cortisol levels (needed for lung maturation)
- Rising levels of placental estrogen (influenced by CRH in a positive feedback loop), drive uterine changes necessary at birth
- Pregnancy duration and timing determined by placental clock (CRH levels)
Corticotrophin-relasing hormone (CRH) and Parturition
- When a critical level of placental CRH is reached, parturition is triggered.
- This critical level ensures the infant is ready for life outside the womb by increasing fetal cortisol needed for lung maturation, along with estrogen changes for uterine changes to support labor
Initiation and Progression of Parturition
- Placental hormones (CRH, DHEA) contribute to fetal lung maturation and uterine changes
- This triggers the onset of parturition
- Active feedback mechanisms regulate uterine contractions and fetal expulsion.
Question
- What hormone increase is the best indicator of ovulation in a healthy 31-year-old woman trying to conceive?
- Luteinizing hormone (LH)
Recommended Reading
- Medical Sciences, by Jeannette Naish
- Endocrine and Reproductive Physiology, by Bruce White, John Harrison, Lisa Mehlman
- Master Medicine: Physiology, by J McGeown
Six Key Pregnancy Hormones
- Human Chorionic Gonadotropin (hCG): Produced by placenta to support corpus luteum initially; for pregnancy detection
- Progesterone: Produced by initial corpus luteum and later by placenta; maintains pregnancy by preventing uterine contractions and supporting other essential functions
- Estrogen: Produced primarily by placenta; stimulates development of uterine lining and mammary ducts
- Prolactin: Produced by pituitary gland; crucial for breast milk production
- Relaxin: Produced by placenta; relaxes connective tissues (especially in pelvis and cervix) to facilitate pregnancy and birth
- Oxytocin: Produced by pituitary gland; stimulates uterine contractions during labor and milk ejection
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Description
Test your knowledge about the processes of fertilization and implantation in early embryonic development. This quiz covers key concepts such as zygote formation, chromosome numbers, and hormonal roles during pregnancy. Assess your understanding of these foundational biological processes through a series of thought-provoking questions.