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Questions and Answers
What is the primary reason why living organisms require a constant energy input?
What is the primary reason why living organisms require a constant energy input?
Which of the following examples best illustrates irreversible energy fixation in living organisms?
Which of the following examples best illustrates irreversible energy fixation in living organisms?
What is the relationship between the first and second laws of thermodynamics in bioenergetics?
What is the relationship between the first and second laws of thermodynamics in bioenergetics?
Why are enzymes essential for efficient metabolic pathways?
Why are enzymes essential for efficient metabolic pathways?
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Which of the following correctly describes ATP's role in coupling energy-releasing and energy-requiring processes?
Which of the following correctly describes ATP's role in coupling energy-releasing and energy-requiring processes?
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Which of these options is an example of a biological process that captures and stores energy?
Which of these options is an example of a biological process that captures and stores energy?
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How is the concept of entropy related to the generation of waste heat in living organisms?
How is the concept of entropy related to the generation of waste heat in living organisms?
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What is the significance of oxidation-reduction reactions in energy generation?
What is the significance of oxidation-reduction reactions in energy generation?
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What type of enzyme is involved in the step of glycolysis that breaks down a 6-carbon molecule into two smaller 3-carbon molecules?
What type of enzyme is involved in the step of glycolysis that breaks down a 6-carbon molecule into two smaller 3-carbon molecules?
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Which of the following molecules is NOT a product of the citric acid cycle?
Which of the following molecules is NOT a product of the citric acid cycle?
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Which of the following molecules carries electrons from glycolysis and the citric acid cycle to the electron transport chain?
Which of the following molecules carries electrons from glycolysis and the citric acid cycle to the electron transport chain?
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What is the key difference between the first and second steps of glycolysis that involve phosphate group addition?
What is the key difference between the first and second steps of glycolysis that involve phosphate group addition?
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What is the role of NADH in glycolysis?
What is the role of NADH in glycolysis?
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How many ATP molecules are produced per glucose molecule during the citric acid cycle?
How many ATP molecules are produced per glucose molecule during the citric acid cycle?
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What is the role of the electron transport chain in cellular respiration?
What is the role of the electron transport chain in cellular respiration?
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Which of the following statements correctly describes the link reaction in cellular respiration?
Which of the following statements correctly describes the link reaction in cellular respiration?
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Why is the citric acid cycle considered a central hub of metabolism?
Why is the citric acid cycle considered a central hub of metabolism?
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What is the role of ATP synthase in oxidative phosphorylation?
What is the role of ATP synthase in oxidative phosphorylation?
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What process allows an organism to obtain energy by oxidizing nutrients?
What process allows an organism to obtain energy by oxidizing nutrients?
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Which term describes the totality of an organism's chemical reactions?
Which term describes the totality of an organism's chemical reactions?
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What is the role of ATP in cellular processes?
What is the role of ATP in cellular processes?
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Which process is characterized by the use of energy to build complex molecules?
Which process is characterized by the use of energy to build complex molecules?
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What do catabolic reactions primarily do?
What do catabolic reactions primarily do?
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In the context of energy, what does 'net energy' refer to?
In the context of energy, what does 'net energy' refer to?
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Which of the following best describes Gibbs free energy?
Which of the following best describes Gibbs free energy?
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What type of energy is primarily associated with metabolic work in cells?
What type of energy is primarily associated with metabolic work in cells?
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What is the primary outcome of glycolysis?
What is the primary outcome of glycolysis?
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How do organisms typically lose energy during metabolic processes?
How do organisms typically lose energy during metabolic processes?
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What does the change in Gibbs free energy, ΔG, indicate about a reaction?
What does the change in Gibbs free energy, ΔG, indicate about a reaction?
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Which equation represents the relationship of change in Gibbs free energy with enthalpy and entropy?
Which equation represents the relationship of change in Gibbs free energy with enthalpy and entropy?
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How do enzymes affect the activation energy (EA) of a reaction?
How do enzymes affect the activation energy (EA) of a reaction?
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What is the primary function of ATP in biological systems?
What is the primary function of ATP in biological systems?
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What type of phosphorylation involves the transfer of a phosphate group during glycolysis?
What type of phosphorylation involves the transfer of a phosphate group during glycolysis?
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Which process is an example of oxidative phosphorylation?
Which process is an example of oxidative phosphorylation?
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What phenomenon describes the movement of ions down their electrochemical gradient across a semipermeable membrane?
What phenomenon describes the movement of ions down their electrochemical gradient across a semipermeable membrane?
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What role does negative feedback play in enzyme regulation?
What role does negative feedback play in enzyme regulation?
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What is the primary ATP yield from fermentation per glucose molecule?
What is the primary ATP yield from fermentation per glucose molecule?
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Which process leads to a significant increase in ATP production due to the presence of oxygen?
Which process leads to a significant increase in ATP production due to the presence of oxygen?
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What is the role of NAD+ in cellular respiration?
What is the role of NAD+ in cellular respiration?
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Which of the following statements about metabolism is correct?
Which of the following statements about metabolism is correct?
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The overall reaction for cellular respiration can be summarized by which equation?
The overall reaction for cellular respiration can be summarized by which equation?
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What forms the 'high-energy' intermediate that aids in ATP synthesis during cellular respiration?
What forms the 'high-energy' intermediate that aids in ATP synthesis during cellular respiration?
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How many ATP molecules are typically produced from the complete cellular respiration of one glucose molecule?
How many ATP molecules are typically produced from the complete cellular respiration of one glucose molecule?
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What is the function of the proton gradient established by the electron transport chain?
What is the function of the proton gradient established by the electron transport chain?
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What is the main function of the electron transport chain in cellular respiration?
What is the main function of the electron transport chain in cellular respiration?
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What is the primary function of NADH in cellular respiration?
What is the primary function of NADH in cellular respiration?
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Which of the following statements about chemiosmosis is TRUE?
Which of the following statements about chemiosmosis is TRUE?
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What is the primary driving force for the movement of protons across the mitochondrial membrane during chemiosmosis?
What is the primary driving force for the movement of protons across the mitochondrial membrane during chemiosmosis?
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How does the electron transport chain contribute to the establishment of a proton gradient?
How does the electron transport chain contribute to the establishment of a proton gradient?
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Why is electronegativity important in the process of cellular respiration?
Why is electronegativity important in the process of cellular respiration?
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Which of the following best describes the role of ATP in cellular respiration?
Which of the following best describes the role of ATP in cellular respiration?
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What is the main difference between NADH and NADPH?
What is the main difference between NADH and NADPH?
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Flashcards
Free Energy
Free Energy
The energy available to do work in a system.
Entropy
Entropy
A measure of disorder or randomness in a system.
ATP Role
ATP Role
ATP couples energy-releasing reactions with energy-requiring processes.
Oxidation-Reduction Reactions
Oxidation-Reduction Reactions
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Glycolysis
Glycolysis
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Krebs Cycle
Krebs Cycle
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First Law of Thermodynamics
First Law of Thermodynamics
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Second Law of Thermodynamics
Second Law of Thermodynamics
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Energy Balance
Energy Balance
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Cellular Respiration
Cellular Respiration
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ATP
ATP
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Metabolism
Metabolism
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Anabolism
Anabolism
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Catabolism
Catabolism
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Gibbs Free Energy
Gibbs Free Energy
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Energy Conversion
Energy Conversion
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Kinetic Energy
Kinetic Energy
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Chemical Energy
Chemical Energy
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Free Energy (G)
Free Energy (G)
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Gibbs Free Energy Equation
Gibbs Free Energy Equation
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Spontaneous Reaction
Spontaneous Reaction
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Enzymes
Enzymes
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Substrate-level Phosphorylation
Substrate-level Phosphorylation
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Oxidative Phosphorylation
Oxidative Phosphorylation
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Chemiosmosis
Chemiosmosis
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Redox potential
Redox potential
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NADH
NADH
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Electronegativity
Electronegativity
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Energy currency
Energy currency
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Electron transport chain
Electron transport chain
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Proton gradient
Proton gradient
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Redox reaction
Redox reaction
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Fermentation
Fermentation
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ATP Production in Respiration
ATP Production in Respiration
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NAD+ Function
NAD+ Function
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Overall Cellular Respiration Equation
Overall Cellular Respiration Equation
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Metabolic Pathways
Metabolic Pathways
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Proton Gradient Role
Proton Gradient Role
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Energy Harvest
Energy Harvest
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Citric Acid Cycle
Citric Acid Cycle
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ATP Production in Glycolysis
ATP Production in Glycolysis
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Link Reaction
Link Reaction
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Study Notes
Cellular Energetics
- Cellular energetics is the study of how cells produce and use energy.
- This is based on the laws of thermodynamics.
- Energy is related to:
- Capture (respiration, photosynthesis)
- Storage (ATP, NADH, proton gradient)
- Conversion (light, chemical, kinetic)
Energy Conservation and Conversion
- First Law of Thermodynamics: Energy cannot be created or destroyed, only transformed from one form into another.
- Second Law of Thermodynamics: Every energy transfer increases the entropy (disorder) of the universe. Energy transfer increases entropy of the universe.
Energy Conservation and Conversion in Biological Systems
- Not all energy can be converted to biologically relevant work.
- Organisms lose some energy to the environment (primarily heat).
- Some energy is fixed irreversibly (e.g., secondary metabolites in plants).
- Living organisms require a constant input of energy.
Energy Balance
- Ecosystems and individual organisms convert energy and experience energy loss/heat.
- Energy flows through a food chain: Sun → Producers → Primary Consumers → Secondary Consumers → Tertiary Consumers → Decomposers.
- Cellular respiration is a metabolic process where organisms obtain energy by oxidizing nutrients, releasing energy and waste products.
Metabolism and ATP Energy Currency
- Metabolism is the totality of an organism's chemical reactions.
- Anabolism: synthesis of complex molecules using energy.
- Catabolism: breakdown of complex molecules to release energy.
- ATP acts as an energy reservoir in cells, used for chemical work, transport, and movement.
Gibbs Free Energy
- Gibbs free energy (G) is the portion of a system's energy that can perform work.
- The change in Gibbs free energy (ΔG) determines if a reaction is spontaneous.
- ΔG = H - TS (where H is enthalpy, T is temperature, and S is entropy).
- A negative ΔG indicates a spontaneous reaction.
Chemical Reactions
- Exergonic reactions release energy (ΔG < 0).
- Endergonic reactions require energy input (ΔG > 0).
- Energy released from exergonic reactions can power endergonic reactions.
Enzymes and Metabolic Regulation
- Enzymes catalyze metabolic reactions in cells.
- Enzymes lower activation energy for reactions to proceed more rapidly.
- Enzymes are influenced by pH and regulated by protein modification (e.g., phosphorylation).
- Enzymes can also be inhibited by the end product of a pathway (negative feedback).
ATP as Energy Currency
- ATP (adenosine triphosphate) releases energy when the outermost inorganic phosphate is cleaved off, producing ADP (adenosine diphosphate).
- The stored chemical energy in ATP results from the electrostatic repulsion of the negative charges in the phosphates.
- ΔG = -7.3 kcal/mol (-30.5 kJ/mol).
Formation of ATP
- Substrate-level phosphorylation: Transfer of a phosphate group from a substrate to ADP to form ATP. (example: Glycolysis).
- Oxidative phosphorylation: ATP synthesis coupled with electron transport (example: inner mitochondrial membrane – chemiosmosis).
- Chemiosmosis: process of generating ATP by moving ions across a semipermeable membrane.
Redox Potential and Electron Transport
- Redox potential (energy stored in redox reactions) reflects the affinity of atoms to release or incorporate electrons.
- Relocating electrons from sugars to oxygen (more electronegative element) releases energy.
- Electron transport chain is a series of redox reactions that transfer electrons.
- NAD+/NADH is a vital electron carrier
NADH
- Nicotinamide adenine dinucleotide (NAD+) is a coenzyme that carries electrons (during redox reactions).
- NADH carries electrons from glycolysis and the Citric acid cycle to the electron transport chain to make ATP.
Energy Harvest: From Electrons to ATP
- Chemiosmosis: a process of ATP synthesis caused by H+ ion moving across membranes.
- Intact membranes are crucial for chemiosmosis.
Energy Harvest: From Electrons to ATP (NADH):
- NADH is a crucial electron carrier involved in the oxidation-reduction processes of electron transport, transferring energy necessary for ATP synthesis.
- H+ gradient across mitochondrial membrane drives ATP synthesis.
Cellular Respiration
- A metabolic process that oxidizes nutrients to produce ATP.
- Involves glycolysis, the Citric Acid Cycle, and Oxidative phosphorylation.
- Different pathways are active at different locations in a cell.
Stages of Cellular Respiration
- Glycolysis: Glucose is broken down to pyruvate. Produces a net of 2 ATP and 2 NADH. Occurrs in the cytosol.
- Pyruvate Oxidation: Pyruvate is transported into the mitochondrial matrix and converted to Acetyl CoA. Produces CO2 and 1 NADH per pyruvate.
- Citric Acid Cycle: Acetyl CoA enters the cycle which generates high energy electrons (carried by NADH and FADH2) and ATP. Two turns of the cycle for each glucose molecule.
- Oxidative Phosphorylation: Oxidizes electron carriers NADH and FADH2, producing a significant amount of ATP via the electron transport chain and chemiosmosis. Occurrs within the inner mitochondrial membrane.
Glycolysis (in detail)
- Glycolysis is the first step of cellular respiration. The steps involved are:
- Energy investment: glucose becomes fructose-1, 6-bisphosphate, consuming 2 ATP
- Energy payoff: 2 NAD+ + 4e- + 4H+ → 2 NADH + 2 H+ and 4 ADP + 4P → 4ATP yielding a net of 2 ATP and 2 NADH.
- Glycolysis occurs in the cytosol
Metabolism, Summary
- Metabolism is the totality of an organism's chemical reactions.
- Pathways are interconnected. Glucose breakdown is crucial for ATP capture.
- Respiration occurs via glycolysis, citric acid cycle, and oxidative phosphorylation.
- Fermentation occurs in low oxygen conditions.
Preparing Pyruvate for Citric Acid Cycle
- Pyruvate is converted to acetyl-CoA which is then ready to enter the citric acid cycle.
- This occurs in the mitochondrial matrix and involves the release of CO2 and the transfer of electrons to NAD+.
- This step is also referred to as the link reaction.
Citric Acid Cycle (in detail)
- Cycle breaks down acetyl CoA, generating high-energy molecules.
- 8 NADH, 2 FADH2, and 2 ATP molecules are produced per glucose molecule via two turns of the citric acid cycle.
Electron Transport Chain and ATP Synthase
- The electron transport chain (ETC) is a sequence of electron carriers.
- Energy released pumps H+ across the mitochondrial membrane creating a proton gradient.
- ATP synthase uses the proton gradient to generate ATP (chemiosmosis).
Electron Transport Chain and ATP Synthase Summary
- Electrons are passed along the chain from NADH and FADH2, releasing energy.
- H+ions are pumped into the intermembrane space creating a H+ gradient.
- The gradient drives H+ through ATP synthase, which produces ATP (chemiosmosis).
Fermentation
- Anaerobic alternative to respiration.
- The process regenerates NAD+ from NADH, so glycolysis can continue.
- Two types fermentation pathway: alcohol and lactic acid.
Overall Reaction of Cellular Respiration
- Glucose + Oxygen → Carbon Dioxide + Water + Energy (ATP)
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Description
Test your understanding of bioenergetics in living organisms with this quiz focused on energy requirements, metabolic pathways, and thermodynamics. Explore the roles of enzymes, ATP, and oxidation-reduction reactions in energy generation. Perfect for students studying biology and bioenergetics at various levels.