Biology Chapter on Bioenergetics
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Questions and Answers

What is the primary reason why living organisms require a constant energy input?

  • To synthesize essential molecules and perform cellular processes.
  • To generate waste products and maintain a stable internal environment.
  • To overcome the loss of energy to the environment due to entropy. (correct)
  • To maintain their internal temperature and prevent heat loss.
  • Which of the following examples best illustrates irreversible energy fixation in living organisms?

  • A flower producing a distinctive fragrance to attract pollinators. (correct)
  • A plant storing energy in the form of starch.
  • A muscle contracting and releasing energy as heat.
  • Glucose being broken down to pyruvate during glycolysis.
  • What is the relationship between the first and second laws of thermodynamics in bioenergetics?

  • The first law describes energy conservation, while the second law explains the inevitable increase in entropy during energy transfer. (correct)
  • The first law determines the amount of energy available for biological work, while the second law regulates the efficiency of energy conversion.
  • The first law dictates the direction of energy flow, while the second law governs the types of energy conversions.
  • The first law states that energy can be lost, while the second law explains how energy conversion increases entropy.
  • Why are enzymes essential for efficient metabolic pathways?

    <p>Enzymes lower the activation energy of reactions, increasing their rate. (A)</p> Signup and view all the answers

    Which of the following correctly describes ATP's role in coupling energy-releasing and energy-requiring processes?

    <p>ATP acts as an intermediate energy carrier, transferring energy from catabolic reactions to anabolic reactions. (D)</p> Signup and view all the answers

    Which of these options is an example of a biological process that captures and stores energy?

    <p>Photosynthesis in plants converting light energy into chemical energy. (C)</p> Signup and view all the answers

    How is the concept of entropy related to the generation of waste heat in living organisms?

    <p>Entropy increases with every energy transfer, leading to some energy being lost as heat. (B)</p> Signup and view all the answers

    What is the significance of oxidation-reduction reactions in energy generation?

    <p>They allow for the transfer of energy through electron carriers. (D)</p> Signup and view all the answers

    What type of enzyme is involved in the step of glycolysis that breaks down a 6-carbon molecule into two smaller 3-carbon molecules?

    <p>Lyase (B)</p> Signup and view all the answers

    Which of the following molecules is NOT a product of the citric acid cycle?

    <p>Glucose (C)</p> Signup and view all the answers

    Which of the following molecules carries electrons from glycolysis and the citric acid cycle to the electron transport chain?

    <p>FADH2 (B), NADH (D)</p> Signup and view all the answers

    What is the key difference between the first and second steps of glycolysis that involve phosphate group addition?

    <p>The first step requires energy in the form of ATP, while the second step does not. (C)</p> Signup and view all the answers

    What is the role of NADH in glycolysis?

    <p>It acts as an electron acceptor, oxidizing a substrate and reducing itself. (C)</p> Signup and view all the answers

    How many ATP molecules are produced per glucose molecule during the citric acid cycle?

    <p>2 (B)</p> Signup and view all the answers

    What is the role of the electron transport chain in cellular respiration?

    <p>To create a proton gradient across the mitochondrial membrane, driving ATP synthesis. (B)</p> Signup and view all the answers

    Which of the following statements correctly describes the link reaction in cellular respiration?

    <p>It occurs in the mitochondria and converts pyruvate to acetyl-CoA. (D)</p> Signup and view all the answers

    Why is the citric acid cycle considered a central hub of metabolism?

    <p>It connects catabolic and anabolic pathways, integrating energy production and biosynthesis. (A)</p> Signup and view all the answers

    What is the role of ATP synthase in oxidative phosphorylation?

    <p>To synthesize ATP using the proton gradient generated by the electron transport chain. (D)</p> Signup and view all the answers

    What process allows an organism to obtain energy by oxidizing nutrients?

    <p>Cellular respiration (D)</p> Signup and view all the answers

    Which term describes the totality of an organism's chemical reactions?

    <p>Metabolism (C)</p> Signup and view all the answers

    What is the role of ATP in cellular processes?

    <p>Energy reservoir (B)</p> Signup and view all the answers

    Which process is characterized by the use of energy to build complex molecules?

    <p>Anabolism (D)</p> Signup and view all the answers

    What do catabolic reactions primarily do?

    <p>Release energy (C)</p> Signup and view all the answers

    In the context of energy, what does 'net energy' refer to?

    <p>Energy produced minus energy lost (C)</p> Signup and view all the answers

    Which of the following best describes Gibbs free energy?

    <p>Energy available to do work in a system (C)</p> Signup and view all the answers

    What type of energy is primarily associated with metabolic work in cells?

    <p>Chemical energy (C)</p> Signup and view all the answers

    What is the primary outcome of glycolysis?

    <p>Breakdown of glucose (D)</p> Signup and view all the answers

    How do organisms typically lose energy during metabolic processes?

    <p>As heat (C)</p> Signup and view all the answers

    What does the change in Gibbs free energy, ΔG, indicate about a reaction?

    <p>The reaction occurs spontaneously if ΔG is negative. (A)</p> Signup and view all the answers

    Which equation represents the relationship of change in Gibbs free energy with enthalpy and entropy?

    <p>ΔG = ΔH - TΔS (D)</p> Signup and view all the answers

    How do enzymes affect the activation energy (EA) of a reaction?

    <p>They lower the activation energy required. (D)</p> Signup and view all the answers

    What is the primary function of ATP in biological systems?

    <p>To release energy for cellular processes. (D)</p> Signup and view all the answers

    What type of phosphorylation involves the transfer of a phosphate group during glycolysis?

    <p>Substrate-level phosphorylation (B)</p> Signup and view all the answers

    Which process is an example of oxidative phosphorylation?

    <p>ATP synthase activity in mitochondria. (D)</p> Signup and view all the answers

    What phenomenon describes the movement of ions down their electrochemical gradient across a semipermeable membrane?

    <p>Chemiosmosis (D)</p> Signup and view all the answers

    What role does negative feedback play in enzyme regulation?

    <p>Inhibits the enzyme by the end product. (C)</p> Signup and view all the answers

    What is the primary ATP yield from fermentation per glucose molecule?

    <p>2 ATP (B)</p> Signup and view all the answers

    Which process leads to a significant increase in ATP production due to the presence of oxygen?

    <p>Oxidative phosphorylation (B)</p> Signup and view all the answers

    What is the role of NAD+ in cellular respiration?

    <p>Captures electrons and delivers them to the electron transport chain (B)</p> Signup and view all the answers

    Which of the following statements about metabolism is correct?

    <p>Metabolism can be both anabolic and catabolic (D)</p> Signup and view all the answers

    The overall reaction for cellular respiration can be summarized by which equation?

    <p>C6H12O6 + 6 O2 → 6 CO2 + 6 H2O ∆G= -2870kJ/mol (A)</p> Signup and view all the answers

    What forms the 'high-energy' intermediate that aids in ATP synthesis during cellular respiration?

    <p>Trans-membrane H+ gradient (C)</p> Signup and view all the answers

    How many ATP molecules are typically produced from the complete cellular respiration of one glucose molecule?

    <p>30-32 ATP (C)</p> Signup and view all the answers

    What is the function of the proton gradient established by the electron transport chain?

    <p>To facilitate ATP synthesis (D)</p> Signup and view all the answers

    What is the main function of the electron transport chain in cellular respiration?

    <p>To generate a proton gradient across the mitochondrial membrane (C)</p> Signup and view all the answers

    What is the primary function of NADH in cellular respiration?

    <p>To donate electrons to the electron transport chain (C)</p> Signup and view all the answers

    Which of the following statements about chemiosmosis is TRUE?

    <p>It requires the presence of an intact membrane to establish a proton gradient (C)</p> Signup and view all the answers

    What is the primary driving force for the movement of protons across the mitochondrial membrane during chemiosmosis?

    <p>The concentration gradient of protons (C)</p> Signup and view all the answers

    How does the electron transport chain contribute to the establishment of a proton gradient?

    <p>By using the energy released during electron transfer to pump protons across the membrane (B)</p> Signup and view all the answers

    Why is electronegativity important in the process of cellular respiration?

    <p>It dictates the direction of electron flow in the electron transport chain (B)</p> Signup and view all the answers

    Which of the following best describes the role of ATP in cellular respiration?

    <p>The primary energy source for the process (A)</p> Signup and view all the answers

    What is the main difference between NADH and NADPH?

    <p>NADH donates electrons to the electron transport chain, while NADPH donates electrons to anabolic reactions (C)</p> Signup and view all the answers

    Flashcards

    Free Energy

    The energy available to do work in a system.

    Entropy

    A measure of disorder or randomness in a system.

    ATP Role

    ATP couples energy-releasing reactions with energy-requiring processes.

    Oxidation-Reduction Reactions

    Reactions that involve the transfer of electrons, key in energy generation.

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    Glycolysis

    The metabolic pathway that breaks down glucose to produce energy.

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    Krebs Cycle

    A series of reactions that produce energy carriers from acetyl-CoA.

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    First Law of Thermodynamics

    Energy cannot be created or destroyed, only transformed.

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    Second Law of Thermodynamics

    Energy transfers increase the total entropy of the Universe.

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    Energy Balance

    The balance between energy intake and energy expenditure in an ecosystem or organism.

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    Cellular Respiration

    Metabolic process where organisms convert nutrients into energy, producing waste.

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    ATP

    Adenosine triphosphate, the primary energy currency of the cell used for work.

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    Metabolism

    Total chemical reactions within an organism, including catabolism and anabolism.

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    Anabolism

    Metabolic process that uses energy to synthesize complex molecules from simpler ones.

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    Catabolism

    Metabolic process that breaks down molecules to release energy.

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    Gibbs Free Energy

    Energy available in a system to do work, balancing system and surroundings.

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    Energy Conversion

    The process of changing energy from one form to another, like food to ATP.

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    Kinetic Energy

    The energy of motion; can be associated with work done by an organism.

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    Chemical Energy

    Energy stored in the bonds of chemical compounds, released during reactions.

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    Free Energy (G)

    The portion of a system’s energy that can perform work.

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    Gibbs Free Energy Equation

    ΔG = ΔH - TΔS; relates changes in enthalpy, temperature, and entropy.

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    Spontaneous Reaction

    A reaction that occurs if ΔG is negative, indicating a decrease in free energy.

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    Enzymes

    Proteins that catalyze reactions by lowering activation energy.

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    Substrate-level Phosphorylation

    Direct transfer of a phosphate group to ADP to form ATP.

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    Oxidative Phosphorylation

    Process of ATP formation through chemiosmosis and electron transport chain.

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    Chemiosmosis

    Movement of ions down their electrochemical gradient across a membrane.

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    Redox potential

    Reflects the affinity of atoms to release or incorporate electrons and protons.

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    NADH

    A molecule that serves as an electron currency in cells, derived from NAD+.

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    Electronegativity

    A measure of an atom's ability to attract electrons in a bond.

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    Energy currency

    Molecules like ATP that store and provide energy in biological systems.

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    Electron transport chain

    A series of proteins in the membrane that transfer electrons and pump protons.

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    Proton gradient

    A difference in proton concentration across a membrane used for ATP production.

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    Redox reaction

    A chemical reaction in which one substance is oxidized and another is reduced.

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    Fermentation

    An anaerobic process that converts glucose into 2 ATP.

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    ATP Production in Respiration

    Cellular respiration produces approximately 30-32 ATP per glucose molecule.

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    NAD+ Function

    NAD+ captures electrons and transfers them as NADH to the electron transport chain.

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    Overall Cellular Respiration Equation

    C6H12O6 + 6 O2 → 6 CO2 + 6 H2O; releases energy.

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    Metabolic Pathways

    Biochemical reactions organized in pathways forming complex networks.

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    Proton Gradient Role

    Trans-membrane H+ gradient acts as a 'high-energy' intermediate for ATP synthesis.

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    Energy Harvest

    The process of converting energy from electrons to ATP during cellular respiration.

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    Citric Acid Cycle

    Also known as the Krebs cycle, it processes Acetyl CoA to generate NADH, FADH2, and ATP.

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    ATP Production in Glycolysis

    Glycolysis produces a net gain of 2 ATP from breaking down glucose.

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    Link Reaction

    Converts pyruvate into Acetyl CoA, generating NADH, and occurs before the Citric Acid Cycle.

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    Study Notes

    Cellular Energetics

    • Cellular energetics is the study of how cells produce and use energy.
    • This is based on the laws of thermodynamics.
    • Energy is related to:
      • Capture (respiration, photosynthesis)
      • Storage (ATP, NADH, proton gradient)
      • Conversion (light, chemical, kinetic)

    Energy Conservation and Conversion

    • First Law of Thermodynamics: Energy cannot be created or destroyed, only transformed from one form into another.
    • Second Law of Thermodynamics: Every energy transfer increases the entropy (disorder) of the universe. Energy transfer increases entropy of the universe.

    Energy Conservation and Conversion in Biological Systems

    • Not all energy can be converted to biologically relevant work.
    • Organisms lose some energy to the environment (primarily heat).
    • Some energy is fixed irreversibly (e.g., secondary metabolites in plants).
    • Living organisms require a constant input of energy.

    Energy Balance

    • Ecosystems and individual organisms convert energy and experience energy loss/heat.
    • Energy flows through a food chain: Sun → Producers → Primary Consumers → Secondary Consumers → Tertiary Consumers → Decomposers.
    • Cellular respiration is a metabolic process where organisms obtain energy by oxidizing nutrients, releasing energy and waste products.

    Metabolism and ATP Energy Currency

    • Metabolism is the totality of an organism's chemical reactions.
    • Anabolism: synthesis of complex molecules using energy.
    • Catabolism: breakdown of complex molecules to release energy.
    • ATP acts as an energy reservoir in cells, used for chemical work, transport, and movement.

    Gibbs Free Energy

    • Gibbs free energy (G) is the portion of a system's energy that can perform work.
    • The change in Gibbs free energy (ΔG) determines if a reaction is spontaneous.
    • ΔG = H - TS (where H is enthalpy, T is temperature, and S is entropy).
      • A negative ΔG indicates a spontaneous reaction.

    Chemical Reactions

    • Exergonic reactions release energy (ΔG < 0).
    • Endergonic reactions require energy input (ΔG > 0).
    • Energy released from exergonic reactions can power endergonic reactions.

    Enzymes and Metabolic Regulation

    • Enzymes catalyze metabolic reactions in cells.
    • Enzymes lower activation energy for reactions to proceed more rapidly.
    • Enzymes are influenced by pH and regulated by protein modification (e.g., phosphorylation).
    • Enzymes can also be inhibited by the end product of a pathway (negative feedback).

    ATP as Energy Currency

    • ATP (adenosine triphosphate) releases energy when the outermost inorganic phosphate is cleaved off, producing ADP (adenosine diphosphate).
    • The stored chemical energy in ATP results from the electrostatic repulsion of the negative charges in the phosphates.
      • ΔG = -7.3 kcal/mol (-30.5 kJ/mol).

    Formation of ATP

    • Substrate-level phosphorylation: Transfer of a phosphate group from a substrate to ADP to form ATP. (example: Glycolysis).
    • Oxidative phosphorylation: ATP synthesis coupled with electron transport (example: inner mitochondrial membrane – chemiosmosis).
    • Chemiosmosis: process of generating ATP by moving ions across a semipermeable membrane.

    Redox Potential and Electron Transport

    • Redox potential (energy stored in redox reactions) reflects the affinity of atoms to release or incorporate electrons.
    • Relocating electrons from sugars to oxygen (more electronegative element) releases energy.
    • Electron transport chain is a series of redox reactions that transfer electrons.
    • NAD+/NADH is a vital electron carrier

    NADH

    • Nicotinamide adenine dinucleotide (NAD+) is a coenzyme that carries electrons (during redox reactions).
    • NADH carries electrons from glycolysis and the Citric acid cycle to the electron transport chain to make ATP.

    Energy Harvest: From Electrons to ATP

    • Chemiosmosis: a process of ATP synthesis caused by H+ ion moving across membranes.
    • Intact membranes are crucial for chemiosmosis.

    Energy Harvest: From Electrons to ATP (NADH):

    • NADH is a crucial electron carrier involved in the oxidation-reduction processes of electron transport, transferring energy necessary for ATP synthesis.
    • H+ gradient across mitochondrial membrane drives ATP synthesis.

    Cellular Respiration

    • A metabolic process that oxidizes nutrients to produce ATP.
    • Involves glycolysis, the Citric Acid Cycle, and Oxidative phosphorylation.
    • Different pathways are active at different locations in a cell.

    Stages of Cellular Respiration

    • Glycolysis: Glucose is broken down to pyruvate. Produces a net of 2 ATP and 2 NADH. Occurrs in the cytosol.
    • Pyruvate Oxidation: Pyruvate is transported into the mitochondrial matrix and converted to Acetyl CoA. Produces CO2 and 1 NADH per pyruvate.
    • Citric Acid Cycle: Acetyl CoA enters the cycle which generates high energy electrons (carried by NADH and FADH2) and ATP. Two turns of the cycle for each glucose molecule.
    • Oxidative Phosphorylation: Oxidizes electron carriers NADH and FADH2, producing a significant amount of ATP via the electron transport chain and chemiosmosis. Occurrs within the inner mitochondrial membrane.

    Glycolysis (in detail)

    • Glycolysis is the first step of cellular respiration. The steps involved are:
      • Energy investment: glucose becomes fructose-1, 6-bisphosphate, consuming 2 ATP
      • Energy payoff: 2 NAD+ + 4e- + 4H+ → 2 NADH + 2 H+ and 4 ADP + 4P → 4ATP yielding a net of 2 ATP and 2 NADH.
    • Glycolysis occurs in the cytosol

    Metabolism, Summary

    • Metabolism is the totality of an organism's chemical reactions.
    • Pathways are interconnected. Glucose breakdown is crucial for ATP capture.
    • Respiration occurs via glycolysis, citric acid cycle, and oxidative phosphorylation.
    • Fermentation occurs in low oxygen conditions.

    Preparing Pyruvate for Citric Acid Cycle

    • Pyruvate is converted to acetyl-CoA which is then ready to enter the citric acid cycle.
    • This occurs in the mitochondrial matrix and involves the release of CO2 and the transfer of electrons to NAD+.
    • This step is also referred to as the link reaction.

    Citric Acid Cycle (in detail)

    • Cycle breaks down acetyl CoA, generating high-energy molecules.
    • 8 NADH, 2 FADH2, and 2 ATP molecules are produced per glucose molecule via two turns of the citric acid cycle.

    Electron Transport Chain and ATP Synthase

    • The electron transport chain (ETC) is a sequence of electron carriers.
    • Energy released pumps H+ across the mitochondrial membrane creating a proton gradient.
    • ATP synthase uses the proton gradient to generate ATP (chemiosmosis).

    Electron Transport Chain and ATP Synthase Summary

    • Electrons are passed along the chain from NADH and FADH2, releasing energy.
    • H+ions are pumped into the intermembrane space creating a H+ gradient.
    • The gradient drives H+ through ATP synthase, which produces ATP (chemiosmosis).

    Fermentation

    • Anaerobic alternative to respiration.
    • The process regenerates NAD+ from NADH, so glycolysis can continue.
    • Two types fermentation pathway: alcohol and lactic acid.

    Overall Reaction of Cellular Respiration

    • Glucose + Oxygen → Carbon Dioxide + Water + Energy (ATP)

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