Biology Chapter: Nucleotides and DNA Formation

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What distinguishes ribose from deoxyribose in the nucleotide structure?

  • Presence of a phosphate group
  • Difference in the nitrogen base
  • Presence of a double-ring structure
  • Presence of an OH group on the 2nd carbon (correct)

How are the two strands of DNA oriented in relation to each other?

  • Both strands run in the same direction
  • Base pairs connect the two strands instead of phosphodiester bonds
  • The strands run in an anti-parallel direction (correct)
  • One strand has a 3’ end aligned with the other strand’s 3’ end

Which of the following nitrogen bases is unique to RNA?

  • Thymine
  • Cytosine
  • Adenine
  • Uracil (correct)

What type of bond forms the sugar-phosphate backbone of a DNA strand?

<p>Phosphodiester bonds (A)</p> Signup and view all the answers

Which statement correctly describes the size and structure of purines and pyrimidines?

<p>Purines are large, double-ringed bases (B)</p> Signup and view all the answers

What role do hydrogen bonds play in the structure of DNA?

<p>They connect the nitrogen bases of opposite strands (A)</p> Signup and view all the answers

Which nitrogen bases are classified as pyrimidines in DNA?

<p>Cytosine and Thymine (B)</p> Signup and view all the answers

In the context of DNA, what does the term 'anti-parallel' refer to?

<p>The orientation of the nucleotide sugar-carbon backbones (B)</p> Signup and view all the answers

What is the primary function of the enzyme primase in DNA replication?

<p>To synthesize RNA primers complementary to template strands (A)</p> Signup and view all the answers

Why can DNA polymerase only add nucleotides in a 5’ to 3’ direction?

<p>It requires a 3' hydroxyl group to form a bond (A)</p> Signup and view all the answers

What enzyme is responsible for joining Okazaki fragments on the lagging strand?

<p>DNA ligase (D)</p> Signup and view all the answers

What occurs during the DNA mismatch repair process?

<p>Mistakes are identified, removed, and fixed by DNA polymerase and ligase (A)</p> Signup and view all the answers

Which type of DNA damage is caused by the removal of NH3 from a nitrogen base?

<p>Deamination (D)</p> Signup and view all the answers

What is the role of nuclease enzymes in DNA replication?

<p>To remove RNA primers (C)</p> Signup and view all the answers

What is the consequence of thymine dimer formation?

<p>It stalls DNA replication (D)</p> Signup and view all the answers

What type of mutation can result from depurination?

<p>Deletion mutation (D)</p> Signup and view all the answers

What type of bond forms between adenine and thymine in DNA?

<p>Hydrogen bond (D)</p> Signup and view all the answers

In human chromosomes, how many pairs are categorized as somatic chromosomes?

<p>22 (A)</p> Signup and view all the answers

Which method can be used to distinguish different chromosome pairs?

<p>Fluorescent dyes (D)</p> Signup and view all the answers

What are introns primarily characterized as in gene structure?

<p>Non-coding regions (C)</p> Signup and view all the answers

During which phase are chromosomes highly condensed compared to interphase?

<p>Prophase (C)</p> Signup and view all the answers

What is the principal function of telomeres in chromosomes?

<p>To ensure complete DNA replication (C)</p> Signup and view all the answers

Which type of chromatin is characterized by being highly condensed and inaccessible for transcription?

<p>Heterochromatin (B)</p> Signup and view all the answers

Which component is necessary to form a nucleosome?

<p>Histone octamer (A)</p> Signup and view all the answers

What role does miRNA play in gene expression?

<p>Inhibits translation of mRNA (D)</p> Signup and view all the answers

Flashcards

What are nucleotides?

The basic building blocks of nucleic acids, like DNA and RNA.

What is deoxyribose?

A five-carbon sugar found in DNA, lacking an oxygen atom on the 2' carbon.

What are purines?

Adenine (A) and Guanine (G) are the two types, both with double-ring structures.

What are pyrimidines?

Cytosine (C) and Thymine (T) in DNA, and Uracil (U) in RNA, are all single-ring bases.

Signup and view all the flashcards

What is a phosphodiester bond?

A chemical bond that links nucleotides together in a DNA or RNA strand.

Signup and view all the flashcards

What is the sugar-phosphate backbone?

A long chain of alternating sugar and phosphate groups, forming the backbone of DNA or RNA.

Signup and view all the flashcards

How do the strands of DNA run?

The two DNA strands run in opposite directions, with one strand's 5' end aligning with the other strand's 3' end.

Signup and view all the flashcards

What are hydrogen bonds in DNA?

Weak bonds that hold base pairs together in DNA, specifically between adenine (A) and thymine (T) and between guanine (G) and cytosine (C).

Signup and view all the flashcards

Primer

A short sequence of RNA that is complementary to the template DNA strand. It acts as a starting point for DNA polymerase to begin replication.

Signup and view all the flashcards

Primase

An enzyme that synthesizes RNA primers during DNA replication.

Signup and view all the flashcards

DNA polymerase

An enzyme that reads the template DNA strand and adds complementary nucleotides to create a new DNA strand.

Signup and view all the flashcards

DNA replication

The process of adding nucleotides to a new DNA strand during replication.

Signup and view all the flashcards

Proofreading

A process where DNA polymerase checks for errors in the new DNA strand and corrects them.

Signup and view all the flashcards

5' to 3' direction

The direction in which DNA polymerase adds nucleotides, from the 5th carbon of the sugar molecule to the 3rd carbon.

Signup and view all the flashcards

Leading strand

The strand of DNA that is synthesized continuously during replication.

Signup and view all the flashcards

Lagging strand

The strand of DNA that is synthesized in short fragments, called Okazaki fragments.

Signup and view all the flashcards

Okazaki fragments

Short fragments of DNA synthesized on the lagging strand.

Signup and view all the flashcards

Nuclease

An enzyme that removes RNA primers from the lagging strand.

Signup and view all the flashcards

DNA repair polymerase

An enzyme that fills in the gaps left by removed RNA primers with DNA nucleotides.

Signup and view all the flashcards

DNA ligase

An enzyme that joins the Okazaki fragments together to form a complete DNA strand.

Signup and view all the flashcards

DNA mismatch repair system

A system that scans newly synthesized DNA strands for mistakes and corrects them.

Signup and view all the flashcards

Depurination

The loss of a purine base (Adenine or Guanine) from DNA

Signup and view all the flashcards

Deamination

The removal of an amino group (NH3) from a nitrogenous base in DNA.

Signup and view all the flashcards

Thymine dimer

A type of DNA damage where two adjacent thymine bases bond together, typically caused by UV radiation.

Signup and view all the flashcards

A = T Base Pairing

The two nitrogenous bases Adenine (A) and Thymine (T) form two hydrogen bonds with each other. This is a crucial part of DNA structure and ensures proper base pairing during replication.

Signup and view all the flashcards

C ≡ G Base Pairing

The two nitrogenous bases Cytosine (C) and Guanine (G) form three hydrogen bonds with each other. This is a crucial part of DNA structure and ensures proper base pairing during replication.

Signup and view all the flashcards

Complementary Base Pairing

The specific pairing of nitrogenous bases in DNA, where adenine (A) always pairs with thymine (T) and cytosine (C) always pairs with guanine (G). This ensures the correct sequence of bases is maintained during replication and transcription.

Signup and view all the flashcards

Chromosome

A highly condensed DNA molecule that contains genetic information. In humans, there are 46 chromosomes arranged in 23 pairs.

Signup and view all the flashcards

Autosome

Chromosomes that are not involved in determining sex. Humans have 22 pairs of autosomes.

Signup and view all the flashcards

Sex Chromosome

Chromosomes that determine an individual's sex. In humans, there is one pair, either XX (female) or XY (male).

Signup and view all the flashcards

Homologous Chromosomes

Two versions of the same chromosome, one from each parent. These chromosomes have the same gene loci, but may have different alleles.

Signup and view all the flashcards

Gene

A specific length of DNA that codes for an RNA strand. Genes are the fundamental units of heredity.

Signup and view all the flashcards

Translation

The process by which ribosomes translate RNA into a protein sequence.

Signup and view all the flashcards

Non-protein coding RNA

RNA molecules that do not code for proteins and have various roles in cellular processes.

Signup and view all the flashcards

Intron

Regions within a gene that are transcribed but not translated into protein. These regions are removed from the final mRNA transcript.

Signup and view all the flashcards

Promoter and Regulatory Regions

Regions within a gene that regulate gene expression. These can be promoter or enhancer regions.

Signup and view all the flashcards

Satellite DNA

Repetitive sequences of DNA that are non-protein coding but have specific functions. These include centromeres, telomeres, and replication origins.

Signup and view all the flashcards

Centromere

The constricted region of a chromosome where spindle fibers attach during cell division.

Signup and view all the flashcards

Telomere

The protective caps at the ends of chromosomes that prevent DNA degradation and ensure complete replication. They shorten with every cell division.

Signup and view all the flashcards

Replication Origins

The specific sites on DNA where DNA replication begins.

Signup and view all the flashcards

Study Notes

Nucleotides

  • Nucleotides are the building blocks of nucleic acids (DNA and RNA).
  • Each nucleotide has a pentose sugar (ribose in RNA, deoxyribose in DNA), a nitrogenous base, and a phosphate group.
  • The only difference between ribose and deoxyribose is the presence of an OH group on the 2' carbon in ribose, absent in deoxyribose.
  • Nitrogenous bases are either purines (double-ringed: Adenine (A), Guanine (G)) or pyrimidines (single-ringed: Cytosine (C), Thymine (T) in DNA; Uracil (U) in RNA).

Formation of DNA

  • DNA is a double helix formed by two antiparallel strands of nucleotides.
  • Phosphodiester bonds link nucleotides within each strand (sugar-phosphate backbone).
  • The strands run in opposite 5' to 3' directions.
  • Hydrogen bonds form between complementary base pairs: A=T (2 hydrogen bonds), C≡G (3 hydrogen bonds).
  • Complementary base pairing maintains a constant distance between the two DNA strands.

Chromosomes

  • Chromosomes are highly condensed DNA molecules.
  • Humans have 23 pairs of chromosomes; 22 pairs are somatic (homologous), and one pair are sex chromosomes (XX in females, XY in males).
  • Different chromosome pairs can be distinguished by size, fluorescent dye binding, and staining patterns (e.g., Giemsa stain).
  • Genes are specific DNA segments coding for RNA molecules, which may be translated into proteins or remain as non-protein coding RNA.
  • Chromosomes also contain non-protein coding regions: introns, promotor and regulatory regions.
  • Repeated sequences (satellites) include centromeres (attachment sites for spindle fibers), telomeres (protective caps), and replication origins (DNA replication initiation sites).
  • Repeated sequences can cause mutations.

Structure of Interphase Chromosomes

  • Interphase chromosomes are less condensed than mitotic chromosomes. They're longer and finer, and located within the nucleus.
  • Specific regions of the nucleus are occupied by different chromosomes. Some chromosomes are tethered to the nuclear membrane or nuclear lamina.
  • The nucleolus is formed by the convergence of certain chromosomes (containing genes for rRNA). This is where rRNA is transcribed and ribosomes assemble.
  • Chromatin is the complex of DNA and histone proteins.
  • Nucleosomes are formed when DNA wraps around histone cores ('beads on a string').
  • DNA is further packaged into chromatin fibers, and then into interphase chromosomes.
  • The final condensation step results in the creation of mitotic chromosomes.

Euchromatin vs Heterochromatin

  • Chromatin exists as euchromatin and heterochromatin during interphase.
  • Euchromatin is less condensed which allows transcription. Found in gene-rich regions.
  • Heterochromatin is highly condensed, making transcription impossible. It's located in gene-poor areas, including centromeres.
  • Heterochromatin can silence genes; for example, one X chromosome is inactivated in females.

DNA Replication

  • DNA replication is semi-conservative, where each strand serves as a template.
  • Replication begins at replication origins, typically rich in A=T base pairs.
  • Initiator proteins separate DNA strands.
  • DNA helicase and replication forks open up the DNA helix.
  • Primers are short RNA sequences synthesized by primase, providing a starting point for DNA polymerase.
  • DNA polymerase adds free nucleotides to the 3' end of the primer, following complementary base-pairing rules.
  • Proofreading by DNA polymerase ensures accuracy. Continuous synthesis of the leading strand; lagging strand synthesis leads to Okazaki fragments
  • RNA primers are removed and replaced by DNA by DNA polymerase.
  • DNA ligase seals the gaps between Okazaki fragments, completing the new strands.
  • Mismatch repair systems scan and correct errors.
  • Depurination – spontaneous loss of purine bases.
  • Deamination – spontaneous conversion of a base (e.g. C to U).
  • Dimerization – formation of base dimers (e.g., thymine dimers) due to UV radiation.
  • These damages are repaired by nucleases, DNA repair polymerase and DNA ligase.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

More Like This

Use Quizgecko on...
Browser
Browser