Podcast
Questions and Answers
What distinguishes ribose from deoxyribose in the nucleotide structure?
What distinguishes ribose from deoxyribose in the nucleotide structure?
- Presence of a phosphate group
- Difference in the nitrogen base
- Presence of a double-ring structure
- Presence of an OH group on the 2nd carbon (correct)
How are the two strands of DNA oriented in relation to each other?
How are the two strands of DNA oriented in relation to each other?
- Both strands run in the same direction
- Base pairs connect the two strands instead of phosphodiester bonds
- The strands run in an anti-parallel direction (correct)
- One strand has a 3’ end aligned with the other strand’s 3’ end
Which of the following nitrogen bases is unique to RNA?
Which of the following nitrogen bases is unique to RNA?
- Thymine
- Cytosine
- Adenine
- Uracil (correct)
What type of bond forms the sugar-phosphate backbone of a DNA strand?
What type of bond forms the sugar-phosphate backbone of a DNA strand?
Which statement correctly describes the size and structure of purines and pyrimidines?
Which statement correctly describes the size and structure of purines and pyrimidines?
What role do hydrogen bonds play in the structure of DNA?
What role do hydrogen bonds play in the structure of DNA?
Which nitrogen bases are classified as pyrimidines in DNA?
Which nitrogen bases are classified as pyrimidines in DNA?
In the context of DNA, what does the term 'anti-parallel' refer to?
In the context of DNA, what does the term 'anti-parallel' refer to?
What is the primary function of the enzyme primase in DNA replication?
What is the primary function of the enzyme primase in DNA replication?
Why can DNA polymerase only add nucleotides in a 5’ to 3’ direction?
Why can DNA polymerase only add nucleotides in a 5’ to 3’ direction?
What enzyme is responsible for joining Okazaki fragments on the lagging strand?
What enzyme is responsible for joining Okazaki fragments on the lagging strand?
What occurs during the DNA mismatch repair process?
What occurs during the DNA mismatch repair process?
Which type of DNA damage is caused by the removal of NH3 from a nitrogen base?
Which type of DNA damage is caused by the removal of NH3 from a nitrogen base?
What is the role of nuclease enzymes in DNA replication?
What is the role of nuclease enzymes in DNA replication?
What is the consequence of thymine dimer formation?
What is the consequence of thymine dimer formation?
What type of mutation can result from depurination?
What type of mutation can result from depurination?
What type of bond forms between adenine and thymine in DNA?
What type of bond forms between adenine and thymine in DNA?
In human chromosomes, how many pairs are categorized as somatic chromosomes?
In human chromosomes, how many pairs are categorized as somatic chromosomes?
Which method can be used to distinguish different chromosome pairs?
Which method can be used to distinguish different chromosome pairs?
What are introns primarily characterized as in gene structure?
What are introns primarily characterized as in gene structure?
During which phase are chromosomes highly condensed compared to interphase?
During which phase are chromosomes highly condensed compared to interphase?
What is the principal function of telomeres in chromosomes?
What is the principal function of telomeres in chromosomes?
Which type of chromatin is characterized by being highly condensed and inaccessible for transcription?
Which type of chromatin is characterized by being highly condensed and inaccessible for transcription?
Which component is necessary to form a nucleosome?
Which component is necessary to form a nucleosome?
What role does miRNA play in gene expression?
What role does miRNA play in gene expression?
Flashcards
What are nucleotides?
What are nucleotides?
The basic building blocks of nucleic acids, like DNA and RNA.
What is deoxyribose?
What is deoxyribose?
A five-carbon sugar found in DNA, lacking an oxygen atom on the 2' carbon.
What are purines?
What are purines?
Adenine (A) and Guanine (G) are the two types, both with double-ring structures.
What are pyrimidines?
What are pyrimidines?
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What is a phosphodiester bond?
What is a phosphodiester bond?
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What is the sugar-phosphate backbone?
What is the sugar-phosphate backbone?
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How do the strands of DNA run?
How do the strands of DNA run?
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What are hydrogen bonds in DNA?
What are hydrogen bonds in DNA?
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Primer
Primer
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Primase
Primase
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DNA polymerase
DNA polymerase
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DNA replication
DNA replication
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Proofreading
Proofreading
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5' to 3' direction
5' to 3' direction
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Leading strand
Leading strand
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Lagging strand
Lagging strand
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Okazaki fragments
Okazaki fragments
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Nuclease
Nuclease
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DNA repair polymerase
DNA repair polymerase
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DNA ligase
DNA ligase
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DNA mismatch repair system
DNA mismatch repair system
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Depurination
Depurination
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Deamination
Deamination
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Thymine dimer
Thymine dimer
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A = T Base Pairing
A = T Base Pairing
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C ≡ G Base Pairing
C ≡ G Base Pairing
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Complementary Base Pairing
Complementary Base Pairing
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Chromosome
Chromosome
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Autosome
Autosome
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Sex Chromosome
Sex Chromosome
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Homologous Chromosomes
Homologous Chromosomes
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Gene
Gene
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Translation
Translation
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Non-protein coding RNA
Non-protein coding RNA
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Intron
Intron
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Promoter and Regulatory Regions
Promoter and Regulatory Regions
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Satellite DNA
Satellite DNA
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Centromere
Centromere
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Telomere
Telomere
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Replication Origins
Replication Origins
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Study Notes
Nucleotides
- Nucleotides are the building blocks of nucleic acids (DNA and RNA).
- Each nucleotide has a pentose sugar (ribose in RNA, deoxyribose in DNA), a nitrogenous base, and a phosphate group.
- The only difference between ribose and deoxyribose is the presence of an OH group on the 2' carbon in ribose, absent in deoxyribose.
- Nitrogenous bases are either purines (double-ringed: Adenine (A), Guanine (G)) or pyrimidines (single-ringed: Cytosine (C), Thymine (T) in DNA; Uracil (U) in RNA).
Formation of DNA
- DNA is a double helix formed by two antiparallel strands of nucleotides.
- Phosphodiester bonds link nucleotides within each strand (sugar-phosphate backbone).
- The strands run in opposite 5' to 3' directions.
- Hydrogen bonds form between complementary base pairs: A=T (2 hydrogen bonds), C≡G (3 hydrogen bonds).
- Complementary base pairing maintains a constant distance between the two DNA strands.
Chromosomes
- Chromosomes are highly condensed DNA molecules.
- Humans have 23 pairs of chromosomes; 22 pairs are somatic (homologous), and one pair are sex chromosomes (XX in females, XY in males).
- Different chromosome pairs can be distinguished by size, fluorescent dye binding, and staining patterns (e.g., Giemsa stain).
- Genes are specific DNA segments coding for RNA molecules, which may be translated into proteins or remain as non-protein coding RNA.
- Chromosomes also contain non-protein coding regions: introns, promotor and regulatory regions.
- Repeated sequences (satellites) include centromeres (attachment sites for spindle fibers), telomeres (protective caps), and replication origins (DNA replication initiation sites).
- Repeated sequences can cause mutations.
Structure of Interphase Chromosomes
- Interphase chromosomes are less condensed than mitotic chromosomes. They're longer and finer, and located within the nucleus.
- Specific regions of the nucleus are occupied by different chromosomes. Some chromosomes are tethered to the nuclear membrane or nuclear lamina.
- The nucleolus is formed by the convergence of certain chromosomes (containing genes for rRNA). This is where rRNA is transcribed and ribosomes assemble.
- Chromatin is the complex of DNA and histone proteins.
- Nucleosomes are formed when DNA wraps around histone cores ('beads on a string').
- DNA is further packaged into chromatin fibers, and then into interphase chromosomes.
- The final condensation step results in the creation of mitotic chromosomes.
Euchromatin vs Heterochromatin
- Chromatin exists as euchromatin and heterochromatin during interphase.
- Euchromatin is less condensed which allows transcription. Found in gene-rich regions.
- Heterochromatin is highly condensed, making transcription impossible. It's located in gene-poor areas, including centromeres.
- Heterochromatin can silence genes; for example, one X chromosome is inactivated in females.
DNA Replication
- DNA replication is semi-conservative, where each strand serves as a template.
- Replication begins at replication origins, typically rich in A=T base pairs.
- Initiator proteins separate DNA strands.
- DNA helicase and replication forks open up the DNA helix.
- Primers are short RNA sequences synthesized by primase, providing a starting point for DNA polymerase.
- DNA polymerase adds free nucleotides to the 3' end of the primer, following complementary base-pairing rules.
- Proofreading by DNA polymerase ensures accuracy. Continuous synthesis of the leading strand; lagging strand synthesis leads to Okazaki fragments
- RNA primers are removed and replaced by DNA by DNA polymerase.
- DNA ligase seals the gaps between Okazaki fragments, completing the new strands.
- Mismatch repair systems scan and correct errors.
Non-replication Related DNA Damage
- Depurination – spontaneous loss of purine bases.
- Deamination – spontaneous conversion of a base (e.g. C to U).
- Dimerization – formation of base dimers (e.g., thymine dimers) due to UV radiation.
- These damages are repaired by nucleases, DNA repair polymerase and DNA ligase.
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