Biochem 3.3  Biology Chapter: Ion Channels and Membrane Function

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Questions and Answers

Why can't Na+ ions pass through a potassium channel pore?

  • Na+ ions are larger than K+ ions.
  • Na+ ions have a different charge than K+ ions.
  • Desolvated Na+ ions are too small to form interactions. (correct)
  • Na+ ions cannot desolvate properly.

What drives the net flow of uncharged solutes through a channel?

  • The size of the solute particles.
  • Temperature of the surrounding environment.
  • The ionic charge of solutes.
  • Concentration gradient across the membrane. (correct)

What happens to the charges on either side of a membrane when potassium ions flow out?

  • Positive charges accumulate outside the membrane. (correct)
  • Negative charges build up outside the membrane.
  • Positive charges increase inside the vesicle.
  • The charges on both sides remain neutral.

What occurs when the positive charges outside the membrane resist additional potassium flow?

<p>Potassium ions will be driven back inside the membrane. (D)</p> Signup and view all the answers

What stabilizes potassium ions within the potassium channel?

<p>Residues of the channel protein. (C)</p> Signup and view all the answers

What happens to potassium concentrations across the membrane over time?

<p>The concentrations become equal on both sides. (D)</p> Signup and view all the answers

What role do channels primarily play for charged particles like potassium?

<p>Channels mediate the flow of ions across the membrane. (C)</p> Signup and view all the answers

What occurs during the process of repolarization in a cell?

<p>Potassium flows out of the cell, decreasing the membrane potential. (D)</p> Signup and view all the answers

How do mechanically gated ion channels operate?

<p>They open in response to physical stimuli. (B)</p> Signup and view all the answers

What characterizes carrier proteins compared to channel proteins?

<p>Carrier proteins can move solutes against their electrochemical gradient. (C)</p> Signup and view all the answers

What is the role of aquaporins in a cell's membrane?

<p>They allow the passage of uncharged particles. (A)</p> Signup and view all the answers

What happens to the membrane potential when cells with channels A and B are exposed to a neutral molecule?

<p>The membrane potential increases then decreases. (B)</p> Signup and view all the answers

What does the flow of ions through a channel create across a cell membrane?

<p>Charge buildup (A)</p> Signup and view all the answers

Which equation describes the relationship between ion concentration and membrane potential?

<p>Nernst equation (D)</p> Signup and view all the answers

If the intracellular concentration of a positive ion is greater than its extracellular concentration, what is the sign of the equilibrium potential?

<p>Negative (B)</p> Signup and view all the answers

What happens to the membrane potential when there is increased intracellular anion concentration?

<p>It becomes positive (A)</p> Signup and view all the answers

What elements does the simplified form of the Nernst equation involve at physiological temperature?

<p>R, T, and F (B)</p> Signup and view all the answers

Which ion is represented as having a negative charge in the context of membrane potential?

<p>Chloride (Cl−) (A)</p> Signup and view all the answers

What term describes an ion that cannot cross the membrane?

<p>Nonpermeable ion (C)</p> Signup and view all the answers

What happens to membrane potential when the permeability of sodium (Na+) increases significantly?

<p>Membrane potential becomes less negative or more positive. (A)</p> Signup and view all the answers

What is the value for Faraday's constant used in calculations related to equilibrium potential?

<p>96,500 C/mol (A)</p> Signup and view all the answers

Which factor does NOT influence the actual membrane potential in a cell?

<p>Volume of the cell (C)</p> Signup and view all the answers

In the equation for determining membrane potential, how is the chloride ion ratio structured?

<p>In:Out ratio (D)</p> Signup and view all the answers

What type of channels are always open, allowing specific ions to flow continuously?

<p>Ungated ion channels (A)</p> Signup and view all the answers

Given a vesicle with 100 mM sodium inside and a 10 mM sodium solution outside, what is the goal of the equilibrium potential?

<p>To prevent sodium ions from diffusing out (A)</p> Signup and view all the answers

What is the primary condition for the majority of ion channels before a signal is received?

<p>They remain closed. (C)</p> Signup and view all the answers

How does the initial concentration of Na+ compare inside and outside of a cell at rest?

<p>Extracellular Na+ concentration is greater. (D)</p> Signup and view all the answers

What effect does an increase in potassium (K+) permeability typically have on the membrane potential?

<p>Membrane potential becomes more negative. (B)</p> Signup and view all the answers

In determining membrane potential, which of the following ions is primarily considered along with sodium and potassium?

<p>Chloride (Cl−) (D)</p> Signup and view all the answers

When a channel opens upon signal receipt, what change occurs in the ions?

<p>Ions flow until the gate closes again. (B)</p> Signup and view all the answers

What characteristic is primarily responsible for ungated potassium channels not equalizing potassium concentrations on both sides of the membrane?

<p>Active pumping of potassium into the cytosol (C)</p> Signup and view all the answers

What is the primary action of ligand-gated ion channels when a ligand binds?

<p>They open the gate to allow ion flow. (D)</p> Signup and view all the answers

What occurs when acetylcholine binds to ligand-gated channels on muscle cells?

<p>Sodium ions enter the cell, causing depolarization. (B)</p> Signup and view all the answers

Which of the following describes what happens during desensitization of ligand-gated ion channels?

<p>The channels close even with continued ligand presence. (A)</p> Signup and view all the answers

How do voltage-gated ion channels respond to changes in membrane potential?

<p>They have a threshold that triggers opening at specific potentials. (A)</p> Signup and view all the answers

What effect does the opening of voltage-gated sodium channels have after ligand-gated sodium channels are activated?

<p>It further depolarizes the membrane potential. (D)</p> Signup and view all the answers

What kind of ion flow do intracellular organelle ungated channels typically allow?

<p>Ions to flow between the cytosol and the organelle lumen. (D)</p> Signup and view all the answers

What triggers the inactivation of voltage-gated sodium channels?

<p>Strong membrane depolarization. (D)</p> Signup and view all the answers

What is the general role of potassium channels in maintaining membrane potential?

<p>They help to maintain a resting membrane potential. (B)</p> Signup and view all the answers

Which statement best describes the function of ligand-gated channels in muscle contraction?

<p>They increase sodium influx which leads to depolarization and contraction. (D)</p> Signup and view all the answers

Flashcards

Passive diffusion

A process where a solute moves through a channel from a region of higher concentration to a region of lower concentration.

Electrochemical gradient

The force exerted by charged particles on each other, impacting their movement.

Electrochemical potential

The phenomenon where ions move through a channel, creating a potential difference across the membrane due to the accumulation of charge.

Ion Desolvation

A specific interaction between an ion and channel residues, leading to the removal of water molecules surrounding the ion.

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Ion transport through a channel

The process of an ion moving through a channel from the region of higher concentration to the region of lower concentration, driven by the electrochemical gradient.

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Electrochemical Equilibrium

The condition where the movement of ions through a channel is balanced, resulting in no net flow across the membrane, even if the ion concentrations on both sides are not equal.

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Channel Selectivity

The ability of a channel to selectively allow passage of certain ions, while excluding others, based on their size and charge.

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Resting membrane potential

The voltage difference across a cell membrane at electrochemical equilibrium, where ion movement is balanced.

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Equilibrium potential

The voltage required to maintain an uneven distribution of a specific ion across a membrane, balancing the electrochemical gradient.

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Nernst equation

The formula used to calculate the equilibrium potential for a specific ion, considering its concentration gradient and charge.

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Ion permeability

A measure of how easily an ion can cross the membrane; a value of 0 indicates the ion cannot cross.

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Extracellular ion concentration

The specific ion concentration outside a cell, represented by [X]out in the Nernst equation.

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Intracellular ion concentration

The specific ion concentration inside a cell, represented by [X]in in the Nernst equation.

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Ion flow through a channel

The process by which ions move down their electrochemical gradient, creating a charge separation across the membrane.

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Charge buildup

The accumulation of charge on one side of the membrane due to ion flow, contributing to the resting membrane potential.

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Membrane Permeability

The ability of a membrane to allow specific ions to pass through it.

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Membrane Potential

The difference in electrical potential between the inside and outside of a cell membrane.

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Goldman-Hodgkin-Katz Equation

An equation to calculate the membrane potential, considering the contributions of sodium, potassium, and chloride ions.

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Ungated Ion Channel

An ion channel that is always open, allowing continuous flow of ions.

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Gated Ion Channel

An ion channel that opens in response to a specific signal, allowing controlled ion flow.

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Closed State

The default state of a gated ion channel, where it is closed and no ion flow occurs.

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Open State

The state of a gated ion channel when it is open, allowing ion flow through the membrane.

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Conformational Change

The process of changing the shape of an ion channel to open or close it.

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Leak Channels

Ions constantly flowing through ungated ion channels, contributing to the resting membrane potential.

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Negative Resting Membrane Potential

The negative charge inside a typical cell compared to the outside.

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Mechanically Gated Ion Channels

These channels open in response to physical stimuli like stretching, enabling sensations such as touch.

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Channels for Uncharged Particles

These channels facilitate the flow of uncharged particles, such as water, across a membrane, crucial for osmosis and regulating osmotic pressure.

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Ion Channels

These channels are responsible for the movement of ions across a membrane, allowing continuous flow when open, unlike carrier proteins.

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Carrier Proteins

These proteins move a specific number of molecules across a membrane each time they operate, and can move solutes either with or against their gradients.

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Ligand-gated ion channels

Channels that open in response to a specific molecule binding to them.

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Channel desensitization

The process where a ligand continuously bound to a channel causes the channel to close even when there is still ligand present.

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Voltage-gated ion channels

Channels that open in response to changes in membrane potential.

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Threshold membrane potential

The specific membrane potential at which a voltage-gated channel opens.

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Depolarization

The process where a membrane potential becomes more positive.

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Hyperpolarization

The process where a membrane potential becomes more negative.

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Potassium pumping

The process where a cell actively pumps potassium ions into the cytosol.

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Potassium leak channels

The process where ungated potassium channels allow potassium ions to flow down their concentration gradient.

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Channel activation cascade

The process where a change in membrane potential caused by one channel triggers the opening of another channel, amplifying the signal.

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Study Notes

Potassium Channel Function

  • Potassium channels selectively allow potassium ions (K+) through, but not sodium ions (Na+), due to specific interactions and size differences
  • Potassium ions dissociate from surrounding water molecules and bind to residues within the pore, while sodium ions are too large or small for stabilization
  • Solutes can move through channels in either direction
  • Direction of movement is influenced by concentration gradients; solutes move from higher to lower concentration for uncharged solutes
  • Channels mediate ion movement, considering charge effects
  • Example: Potassium chloride (KCl) vesicle - potassium flows from higher to lower concentration, creating a charge imbalance

Channel Regulation and Flow

  • Channels facilitate ion passage, and charge imbalances must be considered
  • Channels mediate flow down concentration gradients
  • Initially, both sides of a membrane have a net charge of 0 (e.g., potassium chloride)
  • As potassium flows outward, positive charges build on the outside
  • Simultaneously, negative charges build inside, resisting further potassium flow
  • Eventually, flow rates equalize, even with different concentrations on each side
  • Charge separation creates a counteracting force influencing ion flow based on both concentration and charge
  • Membrane potential is a result of charge separation across a membrane
  • Nernst equation describes equilibrium potential, considering ion concentration and charge

Ion Channels and Membrane Potential

  • Equilibrium potential (E) calculated using the Nernst equation (E = RTzF/ [Xin]/[Xout]), considering the gas constant (R), temperature (T), charge (z), Faraday's constant (F), and intracellular/extracellular ion concentrations
  • Negative equilibrium potential for positive ions with higher intracellular concentration than extracellular concentration.
  • Increased intracellular concentration of a positive ion results in a negative equilibrium potential
  • Equilibrium potential is the membrane voltage required to balance the concentration gradient driving ion movement
  • Imbalance in concentrations causes a voltage difference

Types of Ion Channels

  • Ungated channels allow continuous ion flow
  • Potassium channels are often ungated
  • Ligand-gated channels open in response to a ligand binding to the channel protein.
  • Voltage-gated channels open in response to changes in the membrane potential.
  • Mechanically-gated channels open in response to physical forces.

Carrier Protein Function

  • Carrier proteins transfer solutes across cell membranes, unlike channels that allow continuous flow
  • Movement may be in one direction (uniport), or both directions can be involved in their movement (symport or antiport)
  • These proteins can facilitate transport down or against a gradient by utilizing energy (e.g., ATP hydrolysis).
  • Carry polar molecules
  • Example: Aquaporins are carrier proteins that transport water
  • Example: GLUT2 (glucose transporter) moves glucose into cells

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