Podcast
Questions and Answers
Why can't Na+ ions pass through a potassium channel pore?
Why can't Na+ ions pass through a potassium channel pore?
- Na+ ions are larger than K+ ions.
- Na+ ions have a different charge than K+ ions.
- Desolvated Na+ ions are too small to form interactions. (correct)
- Na+ ions cannot desolvate properly.
What drives the net flow of uncharged solutes through a channel?
What drives the net flow of uncharged solutes through a channel?
- The size of the solute particles.
- Temperature of the surrounding environment.
- The ionic charge of solutes.
- Concentration gradient across the membrane. (correct)
What happens to the charges on either side of a membrane when potassium ions flow out?
What happens to the charges on either side of a membrane when potassium ions flow out?
- Positive charges accumulate outside the membrane. (correct)
- Negative charges build up outside the membrane.
- Positive charges increase inside the vesicle.
- The charges on both sides remain neutral.
What occurs when the positive charges outside the membrane resist additional potassium flow?
What occurs when the positive charges outside the membrane resist additional potassium flow?
What stabilizes potassium ions within the potassium channel?
What stabilizes potassium ions within the potassium channel?
What happens to potassium concentrations across the membrane over time?
What happens to potassium concentrations across the membrane over time?
What role do channels primarily play for charged particles like potassium?
What role do channels primarily play for charged particles like potassium?
What occurs during the process of repolarization in a cell?
What occurs during the process of repolarization in a cell?
How do mechanically gated ion channels operate?
How do mechanically gated ion channels operate?
What characterizes carrier proteins compared to channel proteins?
What characterizes carrier proteins compared to channel proteins?
What is the role of aquaporins in a cell's membrane?
What is the role of aquaporins in a cell's membrane?
What happens to the membrane potential when cells with channels A and B are exposed to a neutral molecule?
What happens to the membrane potential when cells with channels A and B are exposed to a neutral molecule?
What does the flow of ions through a channel create across a cell membrane?
What does the flow of ions through a channel create across a cell membrane?
Which equation describes the relationship between ion concentration and membrane potential?
Which equation describes the relationship between ion concentration and membrane potential?
If the intracellular concentration of a positive ion is greater than its extracellular concentration, what is the sign of the equilibrium potential?
If the intracellular concentration of a positive ion is greater than its extracellular concentration, what is the sign of the equilibrium potential?
What happens to the membrane potential when there is increased intracellular anion concentration?
What happens to the membrane potential when there is increased intracellular anion concentration?
What elements does the simplified form of the Nernst equation involve at physiological temperature?
What elements does the simplified form of the Nernst equation involve at physiological temperature?
Which ion is represented as having a negative charge in the context of membrane potential?
Which ion is represented as having a negative charge in the context of membrane potential?
What term describes an ion that cannot cross the membrane?
What term describes an ion that cannot cross the membrane?
What happens to membrane potential when the permeability of sodium (Na+) increases significantly?
What happens to membrane potential when the permeability of sodium (Na+) increases significantly?
What is the value for Faraday's constant used in calculations related to equilibrium potential?
What is the value for Faraday's constant used in calculations related to equilibrium potential?
Which factor does NOT influence the actual membrane potential in a cell?
Which factor does NOT influence the actual membrane potential in a cell?
In the equation for determining membrane potential, how is the chloride ion ratio structured?
In the equation for determining membrane potential, how is the chloride ion ratio structured?
What type of channels are always open, allowing specific ions to flow continuously?
What type of channels are always open, allowing specific ions to flow continuously?
Given a vesicle with 100 mM sodium inside and a 10 mM sodium solution outside, what is the goal of the equilibrium potential?
Given a vesicle with 100 mM sodium inside and a 10 mM sodium solution outside, what is the goal of the equilibrium potential?
What is the primary condition for the majority of ion channels before a signal is received?
What is the primary condition for the majority of ion channels before a signal is received?
How does the initial concentration of Na+ compare inside and outside of a cell at rest?
How does the initial concentration of Na+ compare inside and outside of a cell at rest?
What effect does an increase in potassium (K+) permeability typically have on the membrane potential?
What effect does an increase in potassium (K+) permeability typically have on the membrane potential?
In determining membrane potential, which of the following ions is primarily considered along with sodium and potassium?
In determining membrane potential, which of the following ions is primarily considered along with sodium and potassium?
When a channel opens upon signal receipt, what change occurs in the ions?
When a channel opens upon signal receipt, what change occurs in the ions?
What characteristic is primarily responsible for ungated potassium channels not equalizing potassium concentrations on both sides of the membrane?
What characteristic is primarily responsible for ungated potassium channels not equalizing potassium concentrations on both sides of the membrane?
What is the primary action of ligand-gated ion channels when a ligand binds?
What is the primary action of ligand-gated ion channels when a ligand binds?
What occurs when acetylcholine binds to ligand-gated channels on muscle cells?
What occurs when acetylcholine binds to ligand-gated channels on muscle cells?
Which of the following describes what happens during desensitization of ligand-gated ion channels?
Which of the following describes what happens during desensitization of ligand-gated ion channels?
How do voltage-gated ion channels respond to changes in membrane potential?
How do voltage-gated ion channels respond to changes in membrane potential?
What effect does the opening of voltage-gated sodium channels have after ligand-gated sodium channels are activated?
What effect does the opening of voltage-gated sodium channels have after ligand-gated sodium channels are activated?
What kind of ion flow do intracellular organelle ungated channels typically allow?
What kind of ion flow do intracellular organelle ungated channels typically allow?
What triggers the inactivation of voltage-gated sodium channels?
What triggers the inactivation of voltage-gated sodium channels?
What is the general role of potassium channels in maintaining membrane potential?
What is the general role of potassium channels in maintaining membrane potential?
Which statement best describes the function of ligand-gated channels in muscle contraction?
Which statement best describes the function of ligand-gated channels in muscle contraction?
Flashcards
Passive diffusion
Passive diffusion
A process where a solute moves through a channel from a region of higher concentration to a region of lower concentration.
Electrochemical gradient
Electrochemical gradient
The force exerted by charged particles on each other, impacting their movement.
Electrochemical potential
Electrochemical potential
The phenomenon where ions move through a channel, creating a potential difference across the membrane due to the accumulation of charge.
Ion Desolvation
Ion Desolvation
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Ion transport through a channel
Ion transport through a channel
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Electrochemical Equilibrium
Electrochemical Equilibrium
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Channel Selectivity
Channel Selectivity
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Resting membrane potential
Resting membrane potential
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Equilibrium potential
Equilibrium potential
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Nernst equation
Nernst equation
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Ion permeability
Ion permeability
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Extracellular ion concentration
Extracellular ion concentration
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Intracellular ion concentration
Intracellular ion concentration
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Ion flow through a channel
Ion flow through a channel
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Charge buildup
Charge buildup
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Membrane Permeability
Membrane Permeability
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Membrane Potential
Membrane Potential
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Goldman-Hodgkin-Katz Equation
Goldman-Hodgkin-Katz Equation
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Ungated Ion Channel
Ungated Ion Channel
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Gated Ion Channel
Gated Ion Channel
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Closed State
Closed State
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Open State
Open State
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Conformational Change
Conformational Change
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Leak Channels
Leak Channels
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Negative Resting Membrane Potential
Negative Resting Membrane Potential
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Mechanically Gated Ion Channels
Mechanically Gated Ion Channels
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Channels for Uncharged Particles
Channels for Uncharged Particles
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Ion Channels
Ion Channels
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Carrier Proteins
Carrier Proteins
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Ligand-gated ion channels
Ligand-gated ion channels
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Channel desensitization
Channel desensitization
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Voltage-gated ion channels
Voltage-gated ion channels
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Threshold membrane potential
Threshold membrane potential
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Depolarization
Depolarization
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Hyperpolarization
Hyperpolarization
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Potassium pumping
Potassium pumping
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Potassium leak channels
Potassium leak channels
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Channel activation cascade
Channel activation cascade
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Study Notes
Potassium Channel Function
- Potassium channels selectively allow potassium ions (K+) through, but not sodium ions (Na+), due to specific interactions and size differences
- Potassium ions dissociate from surrounding water molecules and bind to residues within the pore, while sodium ions are too large or small for stabilization
- Solutes can move through channels in either direction
- Direction of movement is influenced by concentration gradients; solutes move from higher to lower concentration for uncharged solutes
- Channels mediate ion movement, considering charge effects
- Example: Potassium chloride (KCl) vesicle - potassium flows from higher to lower concentration, creating a charge imbalance
Channel Regulation and Flow
- Channels facilitate ion passage, and charge imbalances must be considered
- Channels mediate flow down concentration gradients
- Initially, both sides of a membrane have a net charge of 0 (e.g., potassium chloride)
- As potassium flows outward, positive charges build on the outside
- Simultaneously, negative charges build inside, resisting further potassium flow
- Eventually, flow rates equalize, even with different concentrations on each side
- Charge separation creates a counteracting force influencing ion flow based on both concentration and charge
- Membrane potential is a result of charge separation across a membrane
- Nernst equation describes equilibrium potential, considering ion concentration and charge
Ion Channels and Membrane Potential
- Equilibrium potential (E) calculated using the Nernst equation (E = RTzF/ [Xin]/[Xout]), considering the gas constant (R), temperature (T), charge (z), Faraday's constant (F), and intracellular/extracellular ion concentrations
- Negative equilibrium potential for positive ions with higher intracellular concentration than extracellular concentration.
- Increased intracellular concentration of a positive ion results in a negative equilibrium potential
- Equilibrium potential is the membrane voltage required to balance the concentration gradient driving ion movement
- Imbalance in concentrations causes a voltage difference
Types of Ion Channels
- Ungated channels allow continuous ion flow
- Potassium channels are often ungated
- Ligand-gated channels open in response to a ligand binding to the channel protein.
- Voltage-gated channels open in response to changes in the membrane potential.
- Mechanically-gated channels open in response to physical forces.
Carrier Protein Function
- Carrier proteins transfer solutes across cell membranes, unlike channels that allow continuous flow
- Movement may be in one direction (uniport), or both directions can be involved in their movement (symport or antiport)
- These proteins can facilitate transport down or against a gradient by utilizing energy (e.g., ATP hydrolysis).
- Carry polar molecules
- Example: Aquaporins are carrier proteins that transport water
- Example: GLUT2 (glucose transporter) moves glucose into cells
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