Biology Chapter: Cell Structure and Function
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Questions and Answers

Which characteristic of living organisms involves sensitivity to environmental changes?

  • Sensitivity (correct)
  • Cellular organization
  • Energy utilization
  • Ordered complexity
  • What type of organisms are classified under the domain Prokarya?

  • Unicellular organisms without a nucleus (correct)
  • Organisms with membrane-bound organelles
  • Complex multicellular organisms
  • Organisms possessing a nucleus
  • According to the Cell Theory, what is the smallest living unit?

  • Organisms
  • Tissues
  • Cells (correct)
  • Viruses
  • Which component is NOT typically found in prokaryotic cells?

    <p>Nucleus (A)</p> Signup and view all the answers

    What is the primary composition of the bacterial cell wall?

    <p>Peptidoglycan (B)</p> Signup and view all the answers

    What is the main function of the nucleolus in a cell?

    <p>Ribosome and RNA synthesis (B)</p> Signup and view all the answers

    Which part of the Golgi apparatus is responsible for the exit of materials?

    <p>Trans face (A)</p> Signup and view all the answers

    What is the primary role of lysosomes in a cell?

    <p>Digestion of macromolecules (D)</p> Signup and view all the answers

    Which type of endoplasmic reticulum is involved in lipid synthesis?

    <p>Smooth ER (A)</p> Signup and view all the answers

    What process involves the engulfing of another particle by a cell?

    <p>Phagocytosis (A)</p> Signup and view all the answers

    What is the primary structure responsible for protein synthesis within a cell?

    <p>Ribosome (D)</p> Signup and view all the answers

    Mitochondria are known to be involved in which process?

    <p>Oxidative metabolism (C)</p> Signup and view all the answers

    What structural element is primarily involved in maintaining cell shape and facilitating movement?

    <p>Microfilaments (C)</p> Signup and view all the answers

    Which component of the endomembrane system is primarily responsible for packaging and distributing synthesized molecules?

    <p>Golgi apparatus (B)</p> Signup and view all the answers

    According to the endosymbiosis theory, what was engulfed by a cell to form eukaryotes?

    <p>A prokaryote (A)</p> Signup and view all the answers

    What process describes the net diffusion of water across a membrane toward a higher solute concentration?

    <p>Osmosis (A)</p> Signup and view all the answers

    Which type of junction directly connects the cytoskeletons of neighboring cells or to the extracellular matrix (ECM)?

    <p>Adhesive junctions (C)</p> Signup and view all the answers

    What is a characteristic of homologous structures?

    <p>Same evolutionary origin, different structures (A)</p> Signup and view all the answers

    In protein structure, what term describes the final folded shape of a globular protein?

    <p>Tertiary structure (B)</p> Signup and view all the answers

    What type of bond is formed when atoms share two or more valence electrons?

    <p>Covalent bond (B)</p> Signup and view all the answers

    Which substance is formed by linking two monosaccharides together?

    <p>Disaccharide (B)</p> Signup and view all the answers

    What is the role of chaperones in protein biology?

    <p>To assist in protein folding (A)</p> Signup and view all the answers

    How does a buffer function in biological systems?

    <p>Resists changes in pH (A)</p> Signup and view all the answers

    Which of the following best describes triglycerides?

    <p>Made of one glycerol and three fatty acids (D)</p> Signup and view all the answers

    What is the primary energy currency of the cell?

    <p>Adenosine triphosphate (ATP) (C)</p> Signup and view all the answers

    What structure in plant cells allows for communication and connection between adjacent cells?

    <p>Plasmodesmata (C)</p> Signup and view all the answers

    What proteins are responsible for the recognition of self and non-self cells by the immune system?

    <p>MHC proteins (B)</p> Signup and view all the answers

    Which type of transport requires energy and moves substances from low to high concentration?

    <p>Active transport (B)</p> Signup and view all the answers

    Which term describes small changes in influenza virus proteins that can evade vaccine-induced immunity?

    <p>Antigenic drift (D)</p> Signup and view all the answers

    What is the primary reason why ATP is not suitable for long-term energy storage?

    <p>ATP is too reactive and unstable. (A)</p> Signup and view all the answers

    What distinguishes exergonic reactions from endergonic reactions?

    <p>Exergonic reactions release free energy while endergonic store free energy. (B)</p> Signup and view all the answers

    Which of the following accurately describes the role of retroviruses?

    <p>Reverse transcribe RNA into DNA (C)</p> Signup and view all the answers

    Which statement about the 2nd Law of Thermodynamics is true?

    <p>Entropy is a measure of the disorder of a system. (D)</p> Signup and view all the answers

    What type of junction allows for direct communication between animal cells?

    <p>Communicating junctions (B)</p> Signup and view all the answers

    Which of the following correctly describes the role of enzymes in a chemical reaction?

    <p>Enzymes lower the activation energy required for reactions. (C)</p> Signup and view all the answers

    Which component of the cell membrane contributes to its fluidity?

    <p>Cholesterol (C)</p> Signup and view all the answers

    What happens during glycolysis?

    <p>A six-carbon glucose molecule is split into two three-carbon pyruvate molecules. (D)</p> Signup and view all the answers

    What is the primary component of the viral capsid?

    <p>Proteins (D)</p> Signup and view all the answers

    Which characteristic of the Electron Transport Chain is accurate?

    <p>It transfers electrons through a series of linked protein reactions. (C)</p> Signup and view all the answers

    In which condition do cells typically swell due to excess water intake?

    <p>Hypotonic (D)</p> Signup and view all the answers

    What is the main role of NAD+ in cellular respiration?

    <p>To recycle high-energy electrons for continued glycolysis. (D)</p> Signup and view all the answers

    Which of the following accurately describes facilitated diffusion?

    <p>Involves specific carrier proteins (B)</p> Signup and view all the answers

    What describes the process by which a virus hijacks a host cell to reproduce?

    <p>Viral replication (D)</p> Signup and view all the answers

    Which process involves the transfer of a phosphate group directly to ADP to produce ATP?

    <p>Substrate-level phosphorylation. (A)</p> Signup and view all the answers

    What type of solvent typically allows the passage of small polar molecules through the membrane?

    <p>Water (B)</p> Signup and view all the answers

    Which of the following organisms captures energy through photosynthesis?

    <p>Autotrophs. (D)</p> Signup and view all the answers

    What is a primary function of the sodium-potassium pump?

    <p>Maintains sodium and potassium gradients across the membrane (A)</p> Signup and view all the answers

    What is the definition of free energy (G)?

    <p>The energy available to do work in a system. (C)</p> Signup and view all the answers

    Which type of transport protein moves two molecules in opposite directions?

    <p>Antiporter (D)</p> Signup and view all the answers

    What occurs to pyruvate when oxygen is not available?

    <p>It is reduced to ethanol or lactic acid. (B)</p> Signup and view all the answers

    What total yield of ATP is produced per glucose molecule during cellular respiration in eukaryotes?

    <p>30 ATP (C)</p> Signup and view all the answers

    Which enzyme catalyzes the conversion of pyruvate to acetyl-CoA?

    <p>Pyruvate dehydrogenase (A)</p> Signup and view all the answers

    What is produced from each 3-carbon pyruvate molecule during its oxidation?

    <p>1 CO2, 1 Acetyl-CoA, and 1 NADH (C)</p> Signup and view all the answers

    What is the primary function of DNA polymerase during DNA replication?

    <p>To match existing DNA bases with complementary nucleotides (D)</p> Signup and view all the answers

    Which feature distinguishes the leading strand from the lagging strand during DNA replication?

    <p>The leading strand is synthesized continuously, the lagging strand in fragments (A)</p> Signup and view all the answers

    Where does the citric acid cycle occur in eukaryotic cells?

    <p>Mitochondrial matrix (A)</p> Signup and view all the answers

    What is a primary function of the electron transport chain (ETC)?

    <p>Transport electrons and pump protons (C)</p> Signup and view all the answers

    What role does helicase play in DNA replication?

    <p>Unwinds the DNA double helix (B)</p> Signup and view all the answers

    Which product is associated with lactic acid fermentation?

    <p>Lactic acid (A)</p> Signup and view all the answers

    What is the significance of telomerase in eukaryotic cells?

    <p>It maintains the integrity of chromosome ends (B)</p> Signup and view all the answers

    In anaerobic respiration, which molecules can serve as final electron acceptors?

    <p>Inorganic substances like nitrate or sulfate (A)</p> Signup and view all the answers

    Which statement correctly describes the antiparallel nature of DNA strands?

    <p>One strand extends from 3' to 5' and the other from 5' to 3' (C)</p> Signup and view all the answers

    What distinguishes the semiconservative model of DNA replication from other models?

    <p>Each daughter DNA strand contains one parental and one new strand (A)</p> Signup and view all the answers

    During the citric acid cycle, what is regenerated to allow the cycle to continue?

    <p>Oxaloacetate (B)</p> Signup and view all the answers

    Which type of DNA polymerase in E. coli is primarily responsible for DNA replication?

    <p>DNA polymerase III (B)</p> Signup and view all the answers

    Which of the following describes the role of ATP synthase in cellular respiration?

    <p>Generates ATP by utilizing a proton gradient (C)</p> Signup and view all the answers

    What is the function of the sliding clamp during DNA replication?

    <p>To enhance the processivity of DNA polymerase (C)</p> Signup and view all the answers

    What molecule serves as the final electron acceptor in fermentation?

    <p>An organic molecule (A)</p> Signup and view all the answers

    What type of activity do all three DNA polymerases possess, which is essential for proofreading?

    <p>3' to 5' exonuclease activity (D)</p> Signup and view all the answers

    Which reaction occurs in the presence of oxygen during cellular respiration?

    <p>Pyruvate is oxidized to acetyl-CoA. (D)</p> Signup and view all the answers

    What is the main pigment responsible for photosynthesis in plants?

    <p>Chlorophyll a (B)</p> Signup and view all the answers

    Which of the following best describes the primary role of carotenoids in photosynthesis?

    <p>Aid in the absorption of light energy (A)</p> Signup and view all the answers

    What critical process occurs during anaphase I of meiosis that distinguishes it from mitosis?

    <p>Cohesion is lost from the chromosome arms. (A)</p> Signup and view all the answers

    What major feature of meiosis contributes to genetic variation in sexually reproducing populations?

    <p>Independent assortment of chromosomes. (D)</p> Signup and view all the answers

    How does sister chromatid attachment differ between meiosis I and mitosis?

    <p>Sister kinetochores attach to the same pole in meiosis I. (B)</p> Signup and view all the answers

    What suppresses DNA replication between meiosis I and meiosis II?

    <p>The complete loss of Cyclin B. (A)</p> Signup and view all the answers

    What event occurs during prophase I that significantly alters genetic material?

    <p>Crossing over between homologous chromosomes. (A)</p> Signup and view all the answers

    What is the primary function of the antenna complex in photosynthesis?

    <p>Capture photons and channel light energy (C)</p> Signup and view all the answers

    Which photosystem is primarily responsible for generating NADPH?

    <p>Photosystem 1 (B)</p> Signup and view all the answers

    What molecule replenishes the electron 'hole' in chlorophyll after it is excited by light?

    <p>Water (A)</p> Signup and view all the answers

    Which statement describes cyclic photophosphorylation?

    <p>It generates a proton gradient for ATP synthesis (C)</p> Signup and view all the answers

    What is the end product of the Calvin cycle?

    <p>G3P (D)</p> Signup and view all the answers

    What is the role of RuBisCO in the Calvin cycle?

    <p>Catalyze the fixation of CO2 (B)</p> Signup and view all the answers

    During photorespiration, what causes the enzyme RuBisCO to favor the oxidation of RuBP?

    <p>Closed stomata in hot conditions (D)</p> Signup and view all the answers

    In which type of plants does C4 photosynthesis occur?

    <p>Corn and sugarcane (D)</p> Signup and view all the answers

    What unique feature distinguishes CAM plants from C4 plants?

    <p>C4 pathway occurs during the night (B)</p> Signup and view all the answers

    Which product is created from the reduction phase of the Calvin Cycle?

    <p>G3P (D)</p> Signup and view all the answers

    What characterizes the direction of a nucleotide chain in DNA?

    <p>5'-to-3' orientation (C)</p> Signup and view all the answers

    What type of bond connects nucleotides in a DNA strand?

    <p>Phosphodiester bond (B)</p> Signup and view all the answers

    What was Rosalind Franklin known for in the study of DNA?

    <p>Discovering the helical structure via X-ray diffraction (B)</p> Signup and view all the answers

    Which of the following best represents Chargaff's rules in DNA composition?

    <p>A = T and C = G (A)</p> Signup and view all the answers

    What is the primary role of meiosis in sexual reproduction?

    <p>To halve the chromosome number and produce gametes (C)</p> Signup and view all the answers

    During which stage of meiosis does crossing over occur?

    <p>Prophase I (B)</p> Signup and view all the answers

    What term describes the failure of chromosomes to separate properly during meiosis?

    <p>Nondisjunction (D)</p> Signup and view all the answers

    What occurs during anaphase I of meiosis?

    <p>Homologous chromosomes are pulled apart (C)</p> Signup and view all the answers

    How many genetically distinct gametes are produced at the end of meiosis?

    <p>Four (A)</p> Signup and view all the answers

    How do homologous chromosomes differ from each other?

    <p>They may carry different alleles for the same genes. (B)</p> Signup and view all the answers

    What is the result of the second meiotic division?

    <p>Four haploid cells (D)</p> Signup and view all the answers

    What is the significance of independent assortment during meiosis?

    <p>It increases genetic diversity among gametes. (C)</p> Signup and view all the answers

    What is a characteristic of sister chromatids after meiosis I?

    <p>They carry different genetic information. (A)</p> Signup and view all the answers

    What role does synapsis play in meiosis?

    <p>It allows homologous chromosomes to closely associate. (A)</p> Signup and view all the answers

    Which statement best describes the end result of meiosis in animals?

    <p>Four haploid cells that develop into gametes are formed. (D)</p> Signup and view all the answers

    What happens during telophase II of meiosis?

    <p>Four haploid cells are formed. (C)</p> Signup and view all the answers

    What primarily distinguishes leading strand synthesis from lagging strand synthesis?

    <p>Leading strand synthesis occurs continuously, while lagging strand synthesis is discontinuous. (B), Leading strand synthesis requires only one primer, while lagging strand synthesis requires multiple primers. (C)</p> Signup and view all the answers

    Which enzyme is responsible for removing RNA primers during DNA replication?

    <p>DNA Pol I (C)</p> Signup and view all the answers

    What is the role of the FtsZ protein in bacterial cell division?

    <p>To assist in the formation of the septum. (B)</p> Signup and view all the answers

    What characterizes heterochromatin in a chromosome?

    <p>It is highly condensed, gene-poor, and transcriptionally silent. (B)</p> Signup and view all the answers

    Which statement best describes the process of chromatin condensation during the cell cycle?

    <p>Condensed chromosomes are typically visible during prophase. (B)</p> Signup and view all the answers

    What occurs during prophase of mitosis?

    <p>The nuclear envelope begins to break down. (C), Sister chromatids condense into distinct chromosomes. (D)</p> Signup and view all the answers

    What is the purpose of ligase in DNA replication?

    <p>To join Okazaki fragments together. (C)</p> Signup and view all the answers

    What marks the beginning of telophase in cell division?

    <p>Nuclear envelope forms (C)</p> Signup and view all the answers

    Which feature distinguishes eukaryotic chromosomes from prokaryotic chromosomes?

    <p>Eukaryotic chromosomes contain histones. (D)</p> Signup and view all the answers

    What is the function of the cohesin protein during cell division?

    <p>To hold sister chromatids together until anaphase. (A)</p> Signup and view all the answers

    Which process is responsible for the physical division of the cytoplasm in animal cells?

    <p>Cleavage furrow formation (A)</p> Signup and view all the answers

    How does the structure of chromatin influence gene expression?

    <p>Less condensed chromatin is generally more transcriptionally active. (A)</p> Signup and view all the answers

    What is the primary role of cyclin-dependent kinases (Cdks) in the cell cycle?

    <p>To phosphorylate proteins (C)</p> Signup and view all the answers

    What is primarily responsible for the compaction of DNA within a chromosome?

    <p>Interactions with positively charged histone proteins. (D)</p> Signup and view all the answers

    Which checkpoint assesses the success of DNA replication in the cell cycle?

    <p>G2/M checkpoint (A)</p> Signup and view all the answers

    Which phase of the eukaryotic cell cycle involves DNA replication?

    <p>S phase (A)</p> Signup and view all the answers

    What is the function of the Anaphase-Promoting Complex (APC)?

    <p>To trigger anaphase (A)</p> Signup and view all the answers

    What characterizes proto-oncogenes when they become mutated?

    <p>They become oncogenes (C)</p> Signup and view all the answers

    What is the primary characteristic of a chromosome when it is composed of sister chromatids?

    <p>It is compacted and visible during mitosis. (C)</p> Signup and view all the answers

    Which of the following best describes the kinetochores?

    <p>They are attachment sites for microtubules. (D)</p> Signup and view all the answers

    Which gene is notably involved in the G1 checkpoint and plays a role in determining cell fate after DNA damage?

    <p>p53 gene (C)</p> Signup and view all the answers

    What defines a diploid organism?

    <p>Having two complete sets of chromosomes. (A)</p> Signup and view all the answers

    What is the role of growth factors in cell division?

    <p>To trigger intracellular signaling systems (C)</p> Signup and view all the answers

    How does the control of the cell cycle differ in multicellular eukaryotes compared to yeast?

    <p>Eukaryotes have a more complex control system (A)</p> Signup and view all the answers

    What occurs during cytokinesis in plant cells?

    <p>A cell plate forms (C)</p> Signup and view all the answers

    What critical role does the cdc2 gene serve in cell cycle progression?

    <p>Pushing boundaries for mitosis (D)</p> Signup and view all the answers

    What happens to tumor-suppressor genes in the development of cancer?

    <p>Both copies must be mutated to develop cancer (C)</p> Signup and view all the answers

    What is a characteristic of asexual reproduction?

    <p>Generates genetically identical offspring (D)</p> Signup and view all the answers

    What is the significance of whole-genome sequencing in cancer research?

    <p>It provides insights into tumor heterogeneity (C)</p> Signup and view all the answers

    Flashcards

    Prokaryotic Cells

    Simple, single-celled organisms lacking a nucleus, including bacteria and archaea.

    Eukaryotic Cells

    Complex cells with a nucleus and other membrane-bound organelles.

    Cell Theory

    All living things are made of cells, cells are the basic unit of life, and all cells come from pre-existing cells.

    Peptidoglycan

    A polymer found in bacterial cell walls, providing support and structure.

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    Archaea Cell Walls

    Archaea cell walls lack peptidoglycan, a unique difference from bacteria.

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    Nucleolus

    Region of the nucleus where ribosomes and RNA are synthesized.

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    Nucleus envelope

    Double phospholipid bilayer that surrounds the nucleus.

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    Nuclear pore

    Structure that controls the movement of substances into and out of the nucleus.

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    Ribosomes

    Cellular machinery responsible for protein synthesis.

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    Endomembrane system

    Series of interconnected membranes that compartmentalize the eukaryotic cell.

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    Rough ER

    Site of protein synthesis due to ribosomes attached to membranes.

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    Smooth ER

    Site of lipid synthesis, calcium storage, and detoxification.

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    Golgi apparatus

    Organelle that processes, packages, and distributes molecules.

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    Lysosomes

    Membrane-bound vesicles containing enzymes that break down macromolecules.

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    Mitochondria

    Organelle responsible for cellular respiration, producing ATP.

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    Hydrophobic interior

    The inner region of a cell membrane that repels polar molecules but allows non-polar molecules to pass through.

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    Osmosis

    The movement of water across a semi-permeable membrane from an area of high water concentration to an area of low water concentration.

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    Plasmodesmata

    Specialized channels that connect the cytoplasm of adjacent plant cells, allowing for communication and transport.

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    Adhesive junctions

    Connections that attach the cytoskeletons of neighboring cells or to the extracellular matrix, providing structural support.

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    Separate/Tight junctions

    Connections that seal the plasma membrane of adjacent cells, preventing leakage and maintaining separate compartments.

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    Communicating junctions

    Connections that allow for direct communication between cells, either through chemical or electrical signals.

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    Scientific theory

    A well-substantiated explanation of some aspect of the natural world, supported by a large body of evidence from multiple experiments.

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    Homologous structures

    Structures in different organisms that share a common evolutionary origin but may have different functions.

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    Analogous structures

    Structures in different organisms that have similar functions but evolved independently.

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    Gene

    A discrete unit of hereditary information that codes for a specific trait.

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    Cellulose

    A complex carbohydrate that makes up the cell walls of plants and protists.

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    Chitin

    A complex carbohydrate found in the cell walls of fungi, providing structural support.

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    Glycoproteins & Collagen

    Proteins found in animal cell structures, providing support and flexibility.

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    Glycolipids

    Sugars attached to lipids on the cell surface, acting as markers that identify different cell types.

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    MHC Proteins

    Proteins on the cell surface that identify cells as 'self' or 'non-self', crucial for the immune system.

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    Plasmodesmata in Plant Cells

    Specialized openings that connect the cytoplasm of adjacent plant cells, allowing for communication and transport.

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    Virus Structure

    Consists of a core of nucleic acid (DNA or RNA) surrounded by a protein coat called a capsid.

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    Viral Host Range

    The types of organisms a virus can infect.

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    Tissue Tropism

    The specific tissues a virus can infect within its host.

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    Viral Replication

    Process where a virus hijacks a host cell's machinery to create more viruses, often damaging the cell.

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    Helical Virus Shape

    Spiral rod or thread-like shape.

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    Icosahedral Virus Shape

    Almost spherical, resembling a soccer ball.

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    What is energy?

    The capacity to do work.

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    What is kinetic energy?

    Energy of motion.

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    What is potential energy?

    Stored energy.

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    1st Law of Thermodynamics

    Energy cannot be created or destroyed, only transformed from one form to another.

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    2nd Law of Thermodynamics

    Entropy (disorder) always increases in an isolated system. Energy transformations tend to make things less organized and more stable.

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    Free Energy (G)

    The energy available to do work in a system.

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    Endergonic Reaction

    A reaction that requires energy input to occur; positive free energy change (ΔG > 0).

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    Exergonic Reaction

    A reaction that releases energy; negative free energy change (ΔG < 0).

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    Activation Energy

    The extra energy required to destabilize existing bonds and initiate a chemical reaction.

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    Catalyst

    A substance that speeds up a chemical reaction without being consumed or permanently altered.

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    Fermentation

    A metabolic process that occurs when oxygen is not available, where organic molecules act as the final electron acceptor. This process regenerates NAD+ needed for glycolysis.

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    Pyruvate Oxidation

    The process of breaking down pyruvate into acetyl-CoA, CO2, and NADH. This occurs in the presence of oxygen and is a crucial step in cellular respiration.

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    Krebs Cycle

    A series of chemical reactions that oxidize acetyl-CoA, generating ATP, NADH, and FADH2. This cycle is also known as the citric acid cycle.

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    Electron Transport Chain (ETC)

    A series of membrane-bound electron carriers that transfer electrons from NADH and FADH2, generating a proton gradient across the mitochondrial membrane.

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    Chemiosmosis

    The movement of protons across the mitochondrial membrane driven by the proton gradient, powering the synthesis of ATP.

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    ATP Synthase

    An enzyme complex that uses the energy from the proton gradient to synthesize ATP.

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    Anaerobic Respiration

    A type of respiration that uses inorganic molecules other than oxygen as the final electron acceptor.

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    Methogens

    Microorganisms that produce methane (CH4) as a byproduct of anaerobic respiration.

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    Lactic Acid Fermentation

    A type of fermentation that occurs in animal cells, producing lactic acid as a byproduct.

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    Deamination

    The process of removing the amino group (-NH2) from an amino acid.

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    Beta Oxidation

    A process that breaks down fatty acids into acetyl-CoA, generating energy.

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    Photosynthesis

    The process by which plants and algae convert light energy into chemical energy in the form of glucose.

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    Light-Dependent Reactions

    The first stage of photosynthesis where light energy is captured and used to produce ATP and NADPH.

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    Chlorophyll

    A green pigment in plants and algae that absorbs light energy for photosynthesis.

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    Carotenoids

    Pigments that absorb light energy and can protect chlorophyll from damage.

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    Leading Strand Synthesis

    DNA replication process where new DNA is synthesized continuously along the template strand in the 5' to 3' direction.

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    Lagging Strand Synthesis

    DNA replication process where new DNA is synthesized discontinuously in short fragments (Okazaki fragments) on the opposite strand.

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    What is an Okazaki fragment?

    A short segment of DNA synthesized on the lagging strand during DNA replication.

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    DNA Polymerase III

    A DNA polymerase in prokaryotes responsible for the majority of DNA replication.

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    What does DNA Polymerase I do?

    It removes RNA primers and replaces them with DNA during DNA replication.

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    DNA Ligase

    An enzyme that joins Okazaki fragments together, forming a continuous DNA strand.

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    What is a mutagen?

    Any agent that increases the rate of mutation in DNA.

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    Photolyase

    An enzyme that repairs thymine dimers in DNA using visible light.

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    Binary Fission

    A method of asexual reproduction in prokaryotes where a single cell divides into two identical daughter cells.

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    Septation

    The formation of a septum (a dividing wall) in prokaryotic cells during binary fission.

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    FtsZ Protein

    A protein found in most prokaryotes that forms a ring at the site of cell division.

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    Karyotype

    The complete set of chromosomes in an organism, arranged in a specific order.

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    Diploid

    A cell or organism with two complete sets of chromosomes.

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    Haploid

    A cell or organism with one complete set of chromosomes.

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    Chromatin

    The complex of DNA and proteins (histones) that make up chromosomes.

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    Reaction Center

    A transmembrane protein-pigment complex in photosynthesis that absorbs light energy and initiates electron transfer.

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    Antenna Complex

    A network of chlorophyll molecules that captures photons from sunlight and channels them to the reaction center.

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    Primary Photoevent

    The initial step in photosynthesis where a pigment molecule absorbs a photon of light.

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    Charge Separation

    The transfer of energy from the light-absorbing pigment molecule to the reaction center.

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    Cyclic Photophosphorylation

    A process that uses the electron transport chain to generate ATP but not NADPH. It occurs in anoxygenic photosynthesis.

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    Photosystem I

    A photosynthetic system involved in the noncyclic electron transport chain that produces NADPH.

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    Photosystem II

    A photosynthetic system involved in the noncyclic electron transport chain that generates an oxidation potential to split water.

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    Noncyclic Photophosphorylation

    The primary process of photosynthesis that produces both ATP and NADPH to power the Calvin cycle.

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    Calvin Cycle

    The series of biochemical reactions that fix carbon dioxide into sugars using ATP and NADPH produced during the light-dependent reactions.

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    Rubisco

    A key enzyme in the Calvin cycle that catalyzes the addition of carbon dioxide to RuBP.

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    Photorespiration

    A process that occurs in plants when Rubisco reacts with oxygen instead of carbon dioxide, reducing efficiency.

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    CAM Photosynthesis

    A photosynthetic pathway that separates carbon fixation and the Calvin cycle by time, allowing for efficient water use.

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    Gametes

    Reproductive cells (egg and sperm) that carry half the number of chromosomes of a normal body cell.

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    Fertilization

    The fusion of a male gamete (sperm) and a female gamete (egg) to form a diploid zygote.

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    Sex Chromosomes

    Chromosomes that determine an individual's sex.

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    Homologous Chromosomes

    Pairs of chromosomes that carry the same genes, but may have different alleles (versions of the gene) for those traits.

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    Synapsis

    The pairing of homologous chromosomes during prophase I of meiosis.

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    Crossing Over

    The exchange of genetic material between non-sister chromatids of homologous chromosomes during prophase I.

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    Chiasmata

    The points where crossing over occurs between non-sister chromatids of homologous chromosomes.

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    Independent Assortment

    The random separation of homologous chromosomes during anaphase I of meiosis.

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    Nondisjunction

    The failure of chromosomes to separate properly during meiosis, resulting in gametes with an abnormal number of chromosomes.

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    Aneuploidy

    A condition in which there is an abnormal number of chromosomes in a cell, often due to nondisjunction.

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    Anaphase B

    The stage of mitosis where the spindle poles move further apart, elongating the cell.

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    Telophase

    The final stage of mitosis where the nuclear envelope reforms around each set of chromosomes, the spindle apparatus disassembles, and the chromosomes begin to uncoil.

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    Cytokinesis

    The process of dividing the cytoplasm into two daughter cells, completing cell division.

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    Cleavage Furrow

    The indentation in the cell membrane during animal cytokinesis that pinches off the two daughter cells.

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    Cell Plate Formation

    The process by which a new cell wall forms between the two daughter nuclei in plant cytokinesis.

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    MPF

    Maturation-promoting factor, a key protein complex that triggers mitosis.

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    Cyclins

    Proteins that regulate the cell cycle by binding to and activating cyclin-dependent kinases (Cdks).

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    cdc2

    A gene in yeast that encodes a cyclin-dependent kinase (Cdk) critical for cell cycle progression.

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    Cyclin-Dependent Kinases (Cdks)

    Enzymes that phosphorylate proteins, playing a key role in regulating the cell cycle.

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    G1/S Checkpoint

    A key checkpoint where the cell 'decides' to divide and assesses external signals.

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    G2/M Checkpoint

    A checkpoint where the cell commits to mitosis and ensures DNA replication is complete.

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    Late Metaphase (Spindle) Checkpoint

    A checkpoint where the cell ensures all chromosomes are properly attached to the spindle fibers.

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    Anaphase-Promoting Complex (APC)

    A protein complex that triggers anaphase by marking securin for destruction, allowing separase to break down cohesion and separate sister chromatids.

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    Growth Factors

    Signals that stimulate cell division, acting by triggering intracellular signaling systems.

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    Tumor-Suppressor Genes

    Genes that normally prevent uncontrolled cell growth, but when mutated can lead to cancer.

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    Proto-Oncogenes

    Normal cellular genes that can become oncogenes when mutated, causing uncontrolled cell growth.

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    What is the key difference between meiosis and mitosis?

    In meiosis I, sister chromatids remain connected at the centromere throughout the process, while cohesion is lost from the chromosome arms during anaphase I. This allows for the separation of homologous chromosomes, a key event in meiosis.

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    What is the reason for monopolar attachment in meiosis I?

    The structural differences between centromere-kinetochore complexes in meiosis I and mitosis are responsible for the monopolar attachment of sister kinetochores to the same pole.

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    How does replication get suppressed between meiotic divisions?

    The exact mechanisms are unclear, but it involves the loss of cyclin B and prevention of replication initiation complexes from forming. This ensures that DNA replication only occurs once before the two meiotic divisions.

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    Why is genetic variation so important in sexually reproducing organisms?

    Because of the random orientation of chromosomes during meiosis I and crossing over, sexually reproducing organisms have much greater genetic variation than asexually reproducing ones. This variation is essential for evolution and adaptation.

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    What happens during crossing over?

    During prophase I of meiosis, homologous chromosomes exchange pieces, scrambling the genetic material. This process contributes to genetic diversity in offspring.

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    Base Pairing

    The specific pairing of nitrogenous bases in DNA: Adenine (A) pairs with Thymine (T), and Guanine (G) pairs with Cytosine (C).

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    Hydrogen Bonds

    Weak bonds that hold together complementary base pairs in DNA. A-T forms two hydrogen bonds, while G-C forms three.

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    Phosphodiester Backbone

    The sugar-phosphate backbone of a DNA strand, formed by repeating sugar and phosphate units linked by phosphodiester bonds.

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    5'-to-3' Direction

    The direction in which a single DNA strand extends, from the 5' phosphate end to the 3' hydroxyl end.

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    Antiparallel Configuration

    The two strands of DNA run in opposite directions: one 5'-to-3', the other 3'-to-5'.

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    Semiconservative Replication

    The process where each new DNA molecule consists of one original parent strand and one newly synthesized strand.

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    DNA Polymerase

    Enzymes that synthesize new DNA strands by adding complementary nucleotides to an existing strand in the 5'-to-3' direction.

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    Origin of Replication

    The specific site on a DNA molecule where replication begins in prokaryotes. Replication then proceeds in both directions.

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    Leading Strand

    The DNA strand that is synthesized continuously during replication, in the same direction as the replication fork.

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    Lagging Strand

    The DNA strand that is synthesized discontinuously in short fragments (Okazaki fragments) during replication, in the opposite direction to the replication fork.

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    Study Notes

    Living Organisms

    • Characteristics of living organisms include cellular organization, ordered complexity, sensitivity to the environment, growth, development, reproduction, energy utilization, and homeostasis.

    Taxonomy

    • Taxonomy is a system used to name and classify organisms.
    • The highest level of biological classification is the domain.

    Prokaryotes

    • Prokaryotes are organisms with small, single-celled cells lacking a nucleus.
    • Examples include bacteria and archaea.
      • Prokaryotic cells are the simplest organisms.
      • They lack a membrane-bound nucleus.
      • DNA is present in the nucleoid.
      • A cell wall is outside the plasma membrane.
      • Ribosomes are present.
      • They lack common organelles.
      • Bacterial cell walls are composed of peptidoglycan.
      • Archaea cell walls lack peptidoglycan.

    Eukaryotes

    • Eukaryotes are organisms with larger, complex cells that have nuclei.
      • They contain a membrane-bound nucleus.
      • Their cells are more complex than prokaryotic cells.

    Cell Theory

    • All organisms are composed of cells.
    • Cells are the smallest living units.
    • Cells arise only from pre-existing cells.

    Basic Structural Similarities

    • Genetic material
    • Cytoplasm
    • Ribosomes
    • Plasma membrane are found in all living organisms

    Cytoskeleton

    • Molecules related to actin and tublin are found in prokaryotes and influence cell wall shape, strength, and shape.
    • Keeps organelles in fixed location

    Nucleus

    • Nucleus is the repository of genetic information.
    • Nucleolus is the region where ribosomes/RNA synthesis takes place.
    • The nucleus envelope is composed of two phospholipid bilayers.
    • Nuclear pores control the movement of materials in and out of the nucleus.

    Ribosomes

    • Ribosomes are the cell's protein synthesis machinery.
    • Found in all three domains (bacteria, archaea, and eukaryotes).
    • A complex of ribosomal RNA (rRNA) and protein.

    Endomembrane System

    • A series of membranes throughout the cytoplasm in eukaryotic cells.
    • Divides the cell into compartments for different functions.
    • This system is a fundamental distinction between eukaryotes & prokaryotes.
    • Proteins are transported through the endomembrane system.

    Endoplasmic Reticulum (ER)

    • Rough ER: site of protein synthesis, ribosomes attached
    • Smooth ER: lipid synthesis (phospholipids, store calcium), detoxification
    • The longest internal membrane system

    Golgi Apparatus

    • Flattened stacks of interconnected membranes (Golgi bodies).
    • Function in packaging & distribution of molecules.
    • Has a cis (receiving) face near the ER and a trans (shipping) face.

    Lysosomes

    • Membrane-bounded digestive vesicles.
    • Arise from the Golgi apparatus.
    • Contain enzymes that break down macromolecules (garbage disposal).
    • Break down old organelles.
    • Activated by fusing with a vesicle produced by phagocytosis.

    Microbodies

    • Variety of vesicles in eukaryotes that contain enzymes.

    Peroxisomes

    • Microbodies containing enzymes for oxidizing fatty acids.
    • Produce hydrogen peroxide as a byproduct.

    Vacuoles

    • Membrane-bound structures, typically found in plants, fungi, and protists.
    • Central vacuole: involved in cell growth (plants)
    • Contractile vacuole: in some protists for maintaining water balance
    • Storage vacuole: in plants for storage

    Mitochondria

    • Found in all types of eukaryotic cells.
    • Has two membranes (smooth outer and inner folded membrane).
    • Has its own DNA.
    • Has matrix and intermembrane compartments.
    • Embedded proteins carry out oxidative metabolism.

    Chloroplast

    • Present in plants and some other eukaryotic cells.
    • Surrounded by two membranes.
    • Contains chlorophyll (green pigment).
    • Has thylakoids (membrane sacs) and grana (stacked thylakoids).
    • Has its own DNA.

    Microfilaments (Actin)

    • Two protein chains loosely twined together.
    • Involved in contraction, crawling, and pinching movements.

    Microtubules

    • Largest cytoskeletal elements.
    • Dimers of α- and β-tubulin subunits.
    • Facilitate movement of cell and materials.

    Intermediate Filaments

    • Very stable, not usually broken down.
    • Medium size.

    Endosymbiosis Theory

    • Prokaryote engulfed by another cell = eukaryote.

    Eukaryotic Cell Walls

    • Different composition depending on the organism.
    • Plants and protists: cellulose. Fungi: chitin.
    • Animals lack cell walls but have glycoproteins and collagen

    Cell Connections

    • Three main types:
      1. Adhesive junctions
      2. Tight junctions
      3. Communicating junctions

    Cell-to-Cell Interactions

    • Cells make contact, read signals, and respond.

    Glycoproteins/Glycolipids

    • cell-surface markers

    Plasmodesmata

    • Specialized openings in plant cell walls.
    • Connect the cytoplasm of adjoining cells, similar to gap junctions in animal cells.

    Viruses

    • Nucleic acid core surrounded by a protein coat (capsid).
    • No cytoplasm (not a cell).
    • Nucleic acid: DNA or RNA, single or double-stranded, linear or circular, segmented or not.
    • Host range: types of organisms infected.
    • Tissue tropism: viruses may only infect certain tissues in a host.

    Viral Replication

    • Viruses hijack host cellular machinery for replication, transcription and translation
    • End result assembly and release of virus.

    Virus Structure

    • Viral structure types:
      1. Helical: spiral
      2. Icosahedral: almost spherical

    Viral Genomes

    • Can vary greatly in both type of nucleic acid (DNA or RNA) and number of strands (single or double).
    • Most RNA viruses are single-stranded.

    Retroviruses

    • Have a single-stranded RNA genome reverse-transcribed into double-stranded DNA.
    • Employ reverse transcriptase.

    Virus Classification

    • Methods include taxonomy, disease caused, or host infected.
    • Baltimore system sorts viruses based on genome structure & expression.

    Viral Diseases in Humans

    • Categorized as persistent (chronic or latent), or acute
    • Influenzavirus as an example.

    Influenza

    • Examples: 1918-1919 pandemic
    • Subtypes differ in protein spikes (hemagglutinin & neuraminidase).
    • Antigenic drift & shift can cause pandemics.

    Prions

    • Infectious proteins.

    Viroids

    • Tiny naked molecules of circular RNA; use host proteins to replicate.

    Cell Membranes

    • Form a barrier; regulate passage; receive stimuli; composed of a phospholipid bilayer; with proteins embedded.

    Fluid Mosaic Model

    • Proteins float in a fluid bilayer.

    Membrane Components

    • Phospholipid bilayer
    • Transmembrane proteins
    • Interior protein network
    • Cell-surface markers

    Types of Lipids

    • Phospholipids
      • Amphipathic, spontaneously form bilayers.
    • Sphingolipids
    • Cholesterol

    Protein Functions

    • Transport
    • Enzymes
    • Cell-surface receptors
    • Cell-surface identity markers
    • Cell-cell adhesion
    • Attachment to cytoskeleton
    • Affect membrane structure

    Anchoring Molecules

    • Link membrane proteins to the membrane surface: modified lipids (non-polar regions insert into the bilayer, chemical bonds link to proteins).

    Transmembrane Proteins

    • Spanning the lipid bilayer: non-polar regions embedded, polar regions protrude.

    Transmembrane Domains

    • Hydrophobic amino acids arranged in helices.
    • Single transmembrane domain anchors proteins.

    Membrane Pores

    • Non-polar regions create pores in transmembrane proteins for water and small polar molecules to pass

    Membrane Transport

    • Active transport (requires energy, substances move against concentration gradient.
    • Passive transport (no energy needed, substances move down concentration gradient).

    Active Transport Mechanisms

    1. Uniporters (one molecule at a time).
    2. Symporters (two molecules in the same direction).
    3. Antiporters (two molecules in opposite directions).
    4. Coupled transport

    Vesicular Transport (Endocytosis & Exocytosis)

    • Endocytosis: movement INTO cell (requires energy, phagocytosis, pinocytosis, receptor-mediated).
    • Exocytosis: movement OUT of cell (requires energy)

    ATP

    • Energy currency of cells

    Cellular Respiration

    • Series of chemical reactions releasing energy from sugar, producing ATP.

    Sodium-Potassium Pump

    • Direct use of ATP, antiporter action.

    Facilitated Diffusion

    • Uses proteins (channels or carriers) to aid movement across membrane.

    Channel Proteins

    • Hydrophobic interior; "open or close". (aqueous channels) - polar molecules

    Carrier Proteins

    • Bind molecules for assisted passage
    • Movement through diffusion Requires a concentration difference.

    Osmosis

    • Net diffusion of water across membrane towards higher solute concentration.

    Osmolarity

    • Hypertonic (higher solute concentration). Hypotonic (lower solute concentration). Isotonic (equal concentrations).

    Factors Affecting Diffusion

    • Concentration, molecular size, temperature

    Scientific Method

    • Hypothesis: possible explanation tested.
    • Experiment: designed test to validate hypothesis.
    • Control group: baseline for comparison.
    • Experimental groups: different from control group.

    Homologous & Analogous Structures

    • Homologous: same origin, different structure/function.
    • Analogous: different origin, similar function.

    Genes, Genomes, and Atomic Components

    • Gene: discrete info unit. Genome: entire DNA instructions.
    • Ions: unbalanced charged particles. Isotopes: atoms with same element differing neutrons.
    • Half-life: decay time. Valance electrons: outermost energy level electrons.

    Chemical Bonds and Compounds

    • Molecules: groups of atoms; compounds: more than one type of element.
    • Ionic bonds: electron donation; covalent bonds: electron sharing.
    • pH: potential of hydrogen, acids increase H+, bases decrease H+.

    Macromolecules

    • Carbohydrates (1:2:1 ratio C, H, O).
    • Monosaccharides: simple sugars. Disaccharides: two simple sugars linked. Polysaccharides: long chains.
    • Nucleic acids: nucleotides (sugar, phosphate, base). DNA (amino acid codes), RNA (similar to DNA but with ribose).
    • Proteins: amino acids linked by peptide bonds.

    Protein Structure

    - Primary structure: amino acid sequence
    - Secondary structure: peptide backbone interactions
    - Tertiary structure: final folded shape
    - Quaternary structure: multiple polypeptide chain arrangement
    

    Chaperones

    • Proteins that assist in protein folding.
    • Denaturation: protein loses structure and function.

    Lipids

    • Triglycerides: glycerol + 3 fatty acids.
    • Phospholipids: glycerol + 2 fatty acids + phosphate.
    • Micelles & Phospholipid bilayers
    • Waxes: fatty acids + alcohols.
    • Steroids: important lipid molecules (cholesterol, sex hormones).

    Energy and Thermodynamics

    • Energy: capacity to do work.
    • Kinetic energy: energy of motion; potential energy: stored energy.
    • Heat: convenient energy measure.
    • Energy flow: passage of energy through ecosystem (photosynthesis, potential energy, breaking bonds); oxidation (atom/molecule loses electron); reduction (gains electron); Electron transport chain.
    • 1st & 2nd laws of thermodynamics; free energy (G = H - TS), enthalpy (H), entropy (S), exergonic, endergonic reactions; activation energy (raising energy of reacting molecules & lowering activation energy).

    Catalysts and Enzymes

    • Catalysts: lower activation energy of chemical reactions.
    • Enzymes: most are proteins; speed up reactions by binding substrates in active sites.

    Cellular Respiration

    • 4 stages of aerobic respiration: glycolysis, pyruvate oxidation, citric acid cycle, electron transport chain & chemiosmosis
    • Glycolysis: splitting of glucose, produces 2 ATP, 2 NADPH.
    • Pyruvate oxidation: produces Acetyl-CoA, 2 CO2, 2 NADH
    • Krebs Cycle: Oxidizes acetyl group, 6 CO2, 4 ATP, 10 NADH, 2 FADH2
    • ETC/Chemiosmosis: electron carriers generate a proton gradient which in turn used by ATP synthase to make ATP (32 or 30 ATP).

    Oxidation Without O2

    • Anaerobic respiration (inorganic molecules as final electron acceptor)
    • Fermentation (organic molecules as final electron acceptor).

    Photosynthesis

    • Overview: captures energy from the sun (6CO2 + 12H2O + Light = C6H12O6 + 6H2O + 6O2).
    • Stages: light-dependent reactions (capture energy, make ATP & NADPH), light-independent reactions (Calvin Cycle: carbon fixation, reduction, RuBP regeneration).
    • Pigments (Chlorophyll, carotenoids).
    • Photosystems: antenna complex & reaction center.

    Calvin Cycle

    • 3 phases: carbon fixation, reduction, regeneration.
    • Output: G3P (3-carbon sugar formed); used to make glucose, sucrose and starch; cycle runs in reverse during glucose formation.

    Photorespiration

    • Rubisco: enzyme with carboxylation and photorespiration activities; issues with maximizing CO2 uptake in C3 and environmental conditions.

    C4 and CAM Photosynthesis

    • Adaptations for carbon fixation in hot, dry environments
      • C4 plants: spatial separation of CO2 fixation pathways in different cell types.
      • CAM plants: temporal separation of CO2 fixation, with carbon fixation in different conditions.

    DNA Replication

    • Replication requires a template, enzymes, and building blocks of nucleotides.
    • Stages: initiation, elongation, termination.
    • DNA polymerases: main enzyme for building new strands; proofreading functions.
    • Replication fork: point of opening.
    • Leading strand. Lagging strand. Okazaki fragments

    Eukaryotic DNA Replication

    • Multiple origins.
    • Complexity of replication enzymes.

    Telomeres and Telomerase

    • Protective structures at chromosome ends.
    • Enzyme that maintains telomere length.
    • Telomerase activity during development

    DNA Repair Mechanisms

    • Mismatch repair
    • Photorepair (thymine dimers)
    • Excision repair

    Bacterial Cell Division

    • Binary fission

    Prokaryotic & Eukaryotic Chromosomes

    • Chromatin: DNA & protein complex.
    • Eukaryotes: multiple linear chromosomes.
    • Prokaryotes: single circular chromosome.

    Eukaryotic Cell Cycle

    • G1 (gap 1). S (synthesis). G2 (gap 2). M (mitosis). C (cytokinesis).
    • checkpoints: regulate progression.

    Mitosis

    • Stages: prophase, prometaphase, metaphase, anaphase, telophase, cytokinesis.
      • Spindle apparatus. Centromeres. Kinetochores.

    Meiosis

    • Stages: 2 rounds of nuclear division (I, II),
    • Synapsis Recombination (crossing over)
    • Independent assortment
    • Prophase I, Metaphase I, Anaphase I, Telophase I, Prophase II, Metaphase II, Anaphase II, Telophase II.
    • Final result: 4 haploid cells (gametes).

    Meiosis vs Mitosis

    • Differences in chromosomal behavior and outcomes.

    Errors in Meiosis

    • Non-disjunction: failure of chromosome separation,
    • Aneuploidy: abnormal chromosome number.

    Sex Chromosomes

    • X and Y chromosomes.

    Sexual Reproduction

    • Meiosis and fertilization.
    • Variation increased.

    Cell Cycle Control

    • Checkpoints ensure accuracy.
    • MPF (cyclin-dependent kinase), Cyclins, CDKs.

    Cancer

    • Uncontrolled cell growth due to mutations in tumor suppressor genes and proto-oncogenes.

    Asexual Reproduction

    • Clonal reproduction; in organisms like bacteria

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