Questions and Answers
When preparing a wet mount specimen for viewing, the specimen should be covered with:
A change in what component of microscopy influences the apparent size of the objects being viewed?
Magnification
You have successfully focused on an object with the 10x and 40x objective lenses. You have rotated the 100x objective lens into position but are unable to obtain a sharp image. What is most likely to correct this problem?
After focusing on an object with the low power objective lens, you are trying to bring it into focus under the 40x objective lens, but the field of view is very dark. Which of the following may remedy this problem?
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A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen.
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The mechanical stage upper knob causes the stage to move:
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The mechanical stage lower knob causes the stage to move:
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Using oil immersion is useful because:
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What does the term motile mean?
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Study Notes
Wet Mount Specimen Preparation
- A coverslip is essential for covering the specimen in a wet mount to protect it and ensure clear viewing.
Microscopy Basics
- Magnification refers to the change in size of the objects being viewed in microscopy. It is a critical factor in determining how clearly an object can be seen.
High Power Objective Lens Use
- To achieve a sharp image when using a 100x objective lens, immersion oil should be applied before rotating this lens into position for optimal clarity.
Light Intensity Adjustment
- If the field of view appears dark while focusing with a 40x objective lens, increasing the light intensity can enhance visibility.
Preparation Timing for Wet Mounts
- It is not necessary to add dyes and cell fixatives 12 hours prior to viewing a wet mount specimen, allowing for more flexibility in specimen preparation.
Mechanical Stage Operation
- The upper knob of the mechanical stage allows movement of the stage back and forth, facilitating easy positioning of the specimen.
- The lower knob on the mechanical stage enables movement to the right and left, assisting in finer adjustments during observation.
Oil Immersion Technique
- Oil immersion is advantageous as it significantly increases the resolving power, enabling better detail resolution of the specimen.
Definition of Motile
- The term "motile" describes organisms that are capable of movement, an important characteristic in studying cellular behavior.
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Description
Test your knowledge with these flashcards covering key concepts from Chapter 3 of Biology. This set focuses on microscopy techniques and terms essential for understanding specimen preparation and magnification. Enhance your mastery of the subject with this interactive quiz.
