Biology 2581b Week 7 Transposon Mediated Transformation Quiz

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What is the function of inverted repeats in transposon mediated transformation?

Code for the transposase enzyme

Transcribe the coding region

Move to new positions in the genome

Bind to transposase (correct)

Which component of transposon mediated transformation acts as a trans-acting factor?

Coding region

Plasmid

Inverted repeats

Transposase (correct)

What happens to the transposon in transposon mediated transformation?

It codes for inverted repeats

It is cut out of the genome (correct)

It transcribes the coding region

It gets randomly integrated into the genome

How many components are involved in transposon mediated transformation?

Two Signup and view all the answers

Which process involves incorporating a gene and a marker into a viral genome?

Viral mediated transformation Signup and view all the answers

What is the role of inverted repeats in transposon mediated transformation?

Bind to the coding region Signup and view all the answers

What is the process called when viral DNA, containing a specific gene, is used to infect cells and insert the gene into the cell's genome?

Transposon mediated transformation Signup and view all the answers

Which factor recognizes two recombination sites and facilitates a recombination event between them?

Transposase trans-acting factor Signup and view all the answers

In the context of viral mediated transformation, what is the main outcome of the process?

Insertion of the viral genome into the cell's genome Signup and view all the answers

What technique involves the insertion of specific genes into cells through the use of viral particles?

Viral mediated transformation Signup and view all the answers

In site-specific recombination, where does the recombination event typically occur?

In the genome Signup and view all the answers

What is a key feature of transposon mediated transformation?

Inverted repeats cis-acting factor Signup and view all the answers

What is the main factor responsible for the random integration of DNA into the genome during transposon-mediated transformation?

Transposase Signup and view all the answers

In the context of DNA insertion in the genome, what role do inverted repeats play?

Act as a cis-acting factor Signup and view all the answers

Which of the following statements is true regarding DNA injection in vertebrate embryos for genetic engineering purposes?

It can result in misexpression of Antennapedia gene Signup and view all the answers

Which element acts as a cis-acting factor in the context of transposon-mediated transformation?

Inverted repeats Signup and view all the answers

In the process of DNA insertion in the genome, what does the trans-acting factor primarily do?

Catalyzes transposon movement Signup and view all the answers

What technique has made gene knockout more general and accessible compared to homologous recombination and gene knockout in yeast and mice in the past?

CRISPR technology Signup and view all the answers