Biology 2581b Week 7 Transposon Mediated Transformation Quiz

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Questions and Answers

What is the function of inverted repeats in transposon mediated transformation?

  • Code for the transposase enzyme
  • Transcribe the coding region
  • Move to new positions in the genome
  • Bind to transposase (correct)

Which component of transposon mediated transformation acts as a trans-acting factor?

  • Coding region
  • Plasmid
  • Inverted repeats
  • Transposase (correct)

What happens to the transposon in transposon mediated transformation?

  • It codes for inverted repeats
  • It is cut out of the genome (correct)
  • It transcribes the coding region
  • It gets randomly integrated into the genome

How many components are involved in transposon mediated transformation?

<p>Two (D)</p> Signup and view all the answers

Which process involves incorporating a gene and a marker into a viral genome?

<p>Viral mediated transformation (A)</p> Signup and view all the answers

What is the role of inverted repeats in transposon mediated transformation?

<p>Bind to the coding region (B)</p> Signup and view all the answers

What is the process called when viral DNA, containing a specific gene, is used to infect cells and insert the gene into the cell's genome?

<p>Transposon mediated transformation (D)</p> Signup and view all the answers

Which factor recognizes two recombination sites and facilitates a recombination event between them?

<p>Transposase trans-acting factor (A)</p> Signup and view all the answers

In the context of viral mediated transformation, what is the main outcome of the process?

<p>Insertion of the viral genome into the cell's genome (D)</p> Signup and view all the answers

What technique involves the insertion of specific genes into cells through the use of viral particles?

<p>Viral mediated transformation (A)</p> Signup and view all the answers

In site-specific recombination, where does the recombination event typically occur?

<p>In the genome (B)</p> Signup and view all the answers

What is a key feature of transposon mediated transformation?

<p>Inverted repeats cis-acting factor (A)</p> Signup and view all the answers

What is the main factor responsible for the random integration of DNA into the genome during transposon-mediated transformation?

<p>Transposase (B)</p> Signup and view all the answers

In the context of DNA insertion in the genome, what role do inverted repeats play?

<p>Act as a cis-acting factor (D)</p> Signup and view all the answers

Which of the following statements is true regarding DNA injection in vertebrate embryos for genetic engineering purposes?

<p>It can result in misexpression of Antennapedia gene (A)</p> Signup and view all the answers

Which element acts as a cis-acting factor in the context of transposon-mediated transformation?

<p>Inverted repeats (D)</p> Signup and view all the answers

In the process of DNA insertion in the genome, what does the trans-acting factor primarily do?

<p>Catalyzes transposon movement (B)</p> Signup and view all the answers

What technique has made gene knockout more general and accessible compared to homologous recombination and gene knockout in yeast and mice in the past?

<p>CRISPR technology (A)</p> Signup and view all the answers

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