Podcast
Questions and Answers
What is the function of inverted repeats in transposon mediated transformation?
What is the function of inverted repeats in transposon mediated transformation?
- Code for the transposase enzyme
- Transcribe the coding region
- Move to new positions in the genome
- Bind to transposase (correct)
Which component of transposon mediated transformation acts as a trans-acting factor?
Which component of transposon mediated transformation acts as a trans-acting factor?
- Coding region
- Plasmid
- Inverted repeats
- Transposase (correct)
What happens to the transposon in transposon mediated transformation?
What happens to the transposon in transposon mediated transformation?
- It codes for inverted repeats
- It is cut out of the genome (correct)
- It transcribes the coding region
- It gets randomly integrated into the genome
How many components are involved in transposon mediated transformation?
How many components are involved in transposon mediated transformation?
Which process involves incorporating a gene and a marker into a viral genome?
Which process involves incorporating a gene and a marker into a viral genome?
What is the role of inverted repeats in transposon mediated transformation?
What is the role of inverted repeats in transposon mediated transformation?
What is the process called when viral DNA, containing a specific gene, is used to infect cells and insert the gene into the cell's genome?
What is the process called when viral DNA, containing a specific gene, is used to infect cells and insert the gene into the cell's genome?
Which factor recognizes two recombination sites and facilitates a recombination event between them?
Which factor recognizes two recombination sites and facilitates a recombination event between them?
In the context of viral mediated transformation, what is the main outcome of the process?
In the context of viral mediated transformation, what is the main outcome of the process?
What technique involves the insertion of specific genes into cells through the use of viral particles?
What technique involves the insertion of specific genes into cells through the use of viral particles?
In site-specific recombination, where does the recombination event typically occur?
In site-specific recombination, where does the recombination event typically occur?
What is a key feature of transposon mediated transformation?
What is a key feature of transposon mediated transformation?
What is the main factor responsible for the random integration of DNA into the genome during transposon-mediated transformation?
What is the main factor responsible for the random integration of DNA into the genome during transposon-mediated transformation?
In the context of DNA insertion in the genome, what role do inverted repeats play?
In the context of DNA insertion in the genome, what role do inverted repeats play?
Which of the following statements is true regarding DNA injection in vertebrate embryos for genetic engineering purposes?
Which of the following statements is true regarding DNA injection in vertebrate embryos for genetic engineering purposes?
Which element acts as a cis-acting factor in the context of transposon-mediated transformation?
Which element acts as a cis-acting factor in the context of transposon-mediated transformation?
In the process of DNA insertion in the genome, what does the trans-acting factor primarily do?
In the process of DNA insertion in the genome, what does the trans-acting factor primarily do?
What technique has made gene knockout more general and accessible compared to homologous recombination and gene knockout in yeast and mice in the past?
What technique has made gene knockout more general and accessible compared to homologous recombination and gene knockout in yeast and mice in the past?
