Biological Indicators
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Questions and Answers

Which microorganisms are used as biological indicators (BIs)?

  • Non-spore-forming microbes
  • Spore-forming microorganisms (gram-positive bacteria) (correct)
  • Active state spore-forming microbes
  • Gram-negative bacteria

    • Why are spore-forming microorganisms used as BIs?

  • They are more common in a worst-case bioburden situation
  • They are easier to kill during sterilization
  • They are more resistant to sterilization than non-spore-forming microbes (correct)
  • They represent the majority of bioburden on items to be sterilized

    • What does the ability of a biological indicator to mimic worst-case bioburden scenarios validate?

  • The presence of spore-forming microbes
  • The effectiveness of sterilization processes (correct)
  • The accuracy of bioburden estimation
  • The need for additional sterilization methods

    • What is the purpose of using biological indicators in sterilization processes?

    <p>To validate the effectiveness of sterilization processes</p> Signup and view all the answers

    Which microorganisms are more difficult to kill during sterilization?

    <p>Spore-forming microorganisms (gram-positive bacteria)</p> Signup and view all the answers

    Which microorganisms are exponentially more difficult to kill during sterilization than non-spore-forming microbes or spore-forming microbes in an active state?

    <p>Gram-positive bacteria</p> Signup and view all the answers

    Why are spore-forming microorganisms used as biological indicators (BIs)?

    <p>They are more resistant to sterilization than other microorganisms</p> Signup and view all the answers

    What does the ability of a biological indicator to mimic 'worst-case' bioburden scenarios validate?

    <p>The effectiveness of the sterilization process</p> Signup and view all the answers

    What is the main reason for using spore-forming microorganisms as biological indicators (BIs)?

    <p>They are more resistant to sterilization than other microorganisms</p> Signup and view all the answers

    What represents a minimal subset of the actual bioburden on items to be sterilized?

    <p>Spore-forming microbes</p> Signup and view all the answers

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