Biological Indicators
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Questions and Answers

Which microorganisms are used as biological indicators (BIs)?

  • Non-spore-forming microbes
  • Spore-forming microorganisms (gram-positive bacteria) (correct)
  • Active state spore-forming microbes
  • Gram-negative bacteria

Why are spore-forming microorganisms used as BIs?

  • They are more common in a worst-case bioburden situation
  • They are easier to kill during sterilization
  • They are more resistant to sterilization than non-spore-forming microbes (correct)
  • They represent the majority of bioburden on items to be sterilized

What does the ability of a biological indicator to mimic worst-case bioburden scenarios validate?

  • The presence of spore-forming microbes
  • The effectiveness of sterilization processes (correct)
  • The accuracy of bioburden estimation
  • The need for additional sterilization methods

What is the purpose of using biological indicators in sterilization processes?

<p>To validate the effectiveness of sterilization processes (A)</p> Signup and view all the answers

Which microorganisms are more difficult to kill during sterilization?

<p>Spore-forming microorganisms (gram-positive bacteria) (A)</p> Signup and view all the answers

Which microorganisms are exponentially more difficult to kill during sterilization than non-spore-forming microbes or spore-forming microbes in an active state?

<p>Gram-positive bacteria (B)</p> Signup and view all the answers

Why are spore-forming microorganisms used as biological indicators (BIs)?

<p>They are more resistant to sterilization than other microorganisms (D)</p> Signup and view all the answers

What does the ability of a biological indicator to mimic 'worst-case' bioburden scenarios validate?

<p>The effectiveness of the sterilization process (D)</p> Signup and view all the answers

What is the main reason for using spore-forming microorganisms as biological indicators (BIs)?

<p>They are more resistant to sterilization than other microorganisms (B)</p> Signup and view all the answers

What represents a minimal subset of the actual bioburden on items to be sterilized?

<p>Spore-forming microbes (B)</p> Signup and view all the answers

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