Biol110 Quiz 1 Overview
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Questions and Answers

What is the primary focus of the lecture on proteins?

  • Protein denaturation
  • Protein synthesis
  • Protein folding (correct)
  • Protein function
  • Which domain recognizes double- or single-stranded DNA?

  • Protein fold/domain
  • Quaternary structure
  • Leucine zippers
  • Helix-Turn-Helix (correct)
  • What is the main purpose of estimating the percent of ligand occupied sites in a protein?

  • Evaluating protein folding efficiency
  • Identifying protein function
  • Determining protein synthesis rates
  • Assessing ligand binding affinity (correct)
  • Which type of structure is formed by the assembly of individual polypeptides into a larger functional cluster?

    <p>Quaternary structure</p> Signup and view all the answers

    What is the term used to describe a protein domain that independently folds and recognizes DNA?

    <p>Helix-Turn-Helix</p> Signup and view all the answers

    Which level of protein structure refers to the specific arrangement of several secondary structure elements?

    <p>Motifs (folds)</p> Signup and view all the answers

    What is the term used for a molecule that binds to a protein?

    <p>Ligand</p> Signup and view all the answers

    In the lock-and-key model of binding kinetics, what does the 'P' represent?

    <p>Protein</p> Signup and view all the answers

    Which structural motif is known for recognizing double- or single-stranded DNA?

    <p>Helix-Turn-Helix</p> Signup and view all the answers

    What type of binding allows interactions to be reversible and transient?

    <p>Non-covalent binding</p> Signup and view all the answers

    Which mutant protein is a monomeric variant of DsRed?

    <p>mRFP1</p> Signup and view all the answers

    What determines the rate of the forward reaction in binding kinetics?

    <p>[kon]</p> Signup and view all the answers

    What is the purpose of X-ray crystallography in determining the atomic structure of proteins?

    <p>To collect diffraction data</p> Signup and view all the answers

    Which two scientists were jointly awarded the Nobel Prize in Chemistry in 1962 for their studies on the structures of globular proteins?

    <p>John Kendrew and Max Perutz</p> Signup and view all the answers

    What is the role of heme in the structure of myoglobin?

    <p>It is shown in the electron density of only part of the structure</p> Signup and view all the answers

    What is the source of the fluorophore in the green fluorescent protein (GFP)?

    <p>Jellyfish Aequorea victoria</p> Signup and view all the answers

    Which pair correctly matches the individual with their contribution to protein structure studies?

    <p>John Kendrew - building myoglobin molecule</p> Signup and view all the answers

    What are the steps involved in determining the structure of sperm whale myoglobin by X-ray crystallography?

    <p>Collect diffraction data, calculate electron density, fit known amino acid residues into density</p> Signup and view all the answers

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