Biol110 Quiz 1 Overview

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What is the primary focus of the lecture on proteins?

Protein denaturation

Protein synthesis

Protein folding (correct)

Protein function

Which domain recognizes double- or single-stranded DNA?

Protein fold/domain

Quaternary structure

Leucine zippers

Helix-Turn-Helix (correct)

What is the main purpose of estimating the percent of ligand occupied sites in a protein?

Evaluating protein folding efficiency

Identifying protein function

Determining protein synthesis rates

Assessing ligand binding affinity (correct)

Which type of structure is formed by the assembly of individual polypeptides into a larger functional cluster?

Quaternary structure Signup and view all the answers

What is the term used to describe a protein domain that independently folds and recognizes DNA?

Helix-Turn-Helix Signup and view all the answers

Which level of protein structure refers to the specific arrangement of several secondary structure elements?

Motifs (folds) Signup and view all the answers

What is the term used for a molecule that binds to a protein?

Ligand Signup and view all the answers

In the lock-and-key model of binding kinetics, what does the 'P' represent?

Protein Signup and view all the answers

Which structural motif is known for recognizing double- or single-stranded DNA?

Helix-Turn-Helix Signup and view all the answers

What type of binding allows interactions to be reversible and transient?

Non-covalent binding Signup and view all the answers

Which mutant protein is a monomeric variant of DsRed?

mRFP1 Signup and view all the answers

What determines the rate of the forward reaction in binding kinetics?

[kon] Signup and view all the answers

What is the purpose of X-ray crystallography in determining the atomic structure of proteins?

To collect diffraction data Signup and view all the answers

Which two scientists were jointly awarded the Nobel Prize in Chemistry in 1962 for their studies on the structures of globular proteins?

John Kendrew and Max Perutz Signup and view all the answers

What is the role of heme in the structure of myoglobin?

It is shown in the electron density of only part of the structure Signup and view all the answers

What is the source of the fluorophore in the green fluorescent protein (GFP)?

Jellyfish Aequorea victoria Signup and view all the answers

Which pair correctly matches the individual with their contribution to protein structure studies?

John Kendrew - building myoglobin molecule Signup and view all the answers

What are the steps involved in determining the structure of sperm whale myoglobin by X-ray crystallography?

Collect diffraction data, calculate electron density, fit known amino acid residues into density Signup and view all the answers