Bioinformatics Lab Techniques Quiz
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Questions and Answers

What is the purpose of optimizing PCR conditions?

  • To avoid the use of primers
  • To improve the efficiency and specificity of PCR amplification (correct)
  • To increase the concentration of extracted DNA
  • To decrease the yield of PCR products
  • What is the function of the GC-Clamp in the primer sequences?

  • To reduce the PCR cycle conditions
  • To act as a DNA extraction reagent
  • To increase the melting temperature (Tm) of the primers (correct)
  • To inhibit the DNA polymerase activity
  • What is the purpose of agarose-gel electrophoresis in this context?

  • To synthesize new primer sequences
  • To purify the PCR products
  • To determine the DNA extraction efficiency
  • To visualize and analyze the PCR products (correct)
  • Why are different primer sequences used for Bacteria, Fungi, and Archea?

    <p>To ensure specificity for different organisms</p> Signup and view all the answers

    What is the purpose of PCR product purification?

    <p>To concentrate and clean the PCR products</p> Signup and view all the answers

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