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Questions and Answers
What is the purpose of optimizing PCR conditions?
What is the purpose of optimizing PCR conditions?
- To avoid the use of primers
- To improve the efficiency and specificity of PCR amplification (correct)
- To increase the concentration of extracted DNA
- To decrease the yield of PCR products
What is the function of the GC-Clamp in the primer sequences?
What is the function of the GC-Clamp in the primer sequences?
- To reduce the PCR cycle conditions
- To act as a DNA extraction reagent
- To increase the melting temperature (Tm) of the primers (correct)
- To inhibit the DNA polymerase activity
What is the purpose of agarose-gel electrophoresis in this context?
What is the purpose of agarose-gel electrophoresis in this context?
- To synthesize new primer sequences
- To purify the PCR products
- To determine the DNA extraction efficiency
- To visualize and analyze the PCR products (correct)
Why are different primer sequences used for Bacteria, Fungi, and Archea?
Why are different primer sequences used for Bacteria, Fungi, and Archea?
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What is the purpose of PCR product purification?
What is the purpose of PCR product purification?
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