Bioenergetics and ATP Production
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Questions and Answers

What type of glycosidic bond forms the linear chain of glycogen?

  • β-(1,4)
  • α-(1,4) (correct)
  • α-(1,6)
  • β-(1,6)
  • Which molecule is required to activate glucose 1-phosphate before it can be added to glycogen during synthesis?

  • CTP
  • GTP
  • ATP
  • UDP (correct)
  • What is the primary function of glycogen in skeletal muscle?

  • To regulate blood glucose levels for the whole body
  • To provide a rapid supply of glucose for the muscle itself (correct)
  • To serve as a long term energy storage for the whole body
  • To synthesize nucleotides for the muscle
  • What is the approximate percentage of the liver's weight accounted for by glycogen?

    <p>10% (B)</p> Signup and view all the answers

    What is the primary role of liver glycogen?

    <p>To regulate blood glucose levels for the whole body (D)</p> Signup and view all the answers

    What is the fate of glucose 1-phosphate obtained during glycogen degradation?

    <p>It is converted into glucose 6-phosphate (A)</p> Signup and view all the answers

    Which of the following is a main product of the pentose phosphate pathway?

    <p>NADPH (B)</p> Signup and view all the answers

    What is the primary role of ribose 5-phosphate produced in the pentose phosphate pathway?

    <p>To synthesize nucleotides and nucleic acids (A)</p> Signup and view all the answers

    Approximately how many ATP molecules are generated from one molecule of NADH in the electron transport chain?

    <p>2.5 (B)</p> Signup and view all the answers

    Which of the following molecules directly enters the electron transport chain (ETC) at Complex II?

    <p>FADH2 (A)</p> Signup and view all the answers

    In the context of cellular respiration, what is the net ATP production from glycolysis in an oxidative state?

    <p>2 ATP (A)</p> Signup and view all the answers

    What is the primary role of the malate-aspartate shuttle in cellular respiration?

    <p>To convert cytoplasmic NADH to mitochondrial NADH (A)</p> Signup and view all the answers

    How many ATP are produced by the full oxidation of one glucose molecule, considering both substrate-level and oxidative phosphorylation?

    <p>36-38 (A)</p> Signup and view all the answers

    Which process is responsible for synthesizing glucose from non-carbohydrate precursors?

    <p>Gluconeogenesis (A)</p> Signup and view all the answers

    What is the immediate product of glucose inside the cell that is formed by the action of hexokinase?

    <p>Glucose 6-phosphate (C)</p> Signup and view all the answers

    Which enzyme is important in the regulation of gluconeogenesis process?

    <p>Fructose 1,6-bisphosphatase (D)</p> Signup and view all the answers

    Besides diet and gluconeogenesis, what is another primary source for blood glucose?

    <p>Glycogen degradation (D)</p> Signup and view all the answers

    Why is glycogen degradation considered to be a more rapid source of glucose compared to gluconeogenesis?

    <p>Glycogen is stored in a readily accessible form. (C)</p> Signup and view all the answers

    What is the primary role of bioenergetics in biological systems?

    <p>To determine the feasibility and energy transfer of metabolic processes. (D)</p> Signup and view all the answers

    A reaction with a negative ΔG is considered:

    <p>Exergonic and spontaneous. (A)</p> Signup and view all the answers

    Which of the following best represents the net change in free energy (ΔG) for a coupled reaction where ΔG01 = -5 kcal/mol and ΔG02 = +2 kcal/mol?

    <p>-3 kcal/mol (A)</p> Signup and view all the answers

    What is the primary function of the electron transport chain (ETC) in ATP production?

    <p>Capturing electrons from NADH and FADH2 to reduce oxygen and pump H+. (A)</p> Signup and view all the answers

    What is substrate-level phosphorylation?

    <p>The direct transfer of a phosphate group from a substrate to ADP. (D)</p> Signup and view all the answers

    What is the role of NADH and FADH2 in cellular respiration?

    <p>To act as electron carriers for the electron transport chain. (A)</p> Signup and view all the answers

    What is the main purpose of the pentose phosphate pathway?

    <p>To synthesize ribose 5-phosphate and NADPH. (D)</p> Signup and view all the answers

    Which process converts glucose to pyruvate?

    <p>Glycolysis (C)</p> Signup and view all the answers

    Which of the following represents the correct net ATP production of glycolysis assuming no further reactions with the products?

    <p>2 ATP (B)</p> Signup and view all the answers

    What is the function of fermentation in the absence of oxygen?

    <p>To regenerate NAD+ for glycolysis. (D)</p> Signup and view all the answers

    Where does the citric acid cycle (TCA cycle) mainly occur in eukaryotes?

    <p>In the mitochondrial matrix. (D)</p> Signup and view all the answers

    What is the primary function of the citric acid cycle (TCA cycle)?

    <p>To harvest high-energy electrons from carbon fuels. (D)</p> Signup and view all the answers

    The conversion of pyruvate to acetyl-CoA links which two metabolic processes?

    <p>Glycolysis and the TCA cycle (B)</p> Signup and view all the answers

    What is the role of the proton gradient that is formed during the electron transport chain?

    <p>Drives ATP synthesis by powering ATP synthase. (B)</p> Signup and view all the answers

    What are the two stages of glycolysis?

    <p>Investment and Harvest (C)</p> Signup and view all the answers

    Flashcards

    What is the Electron Transport Chain (ETC)?

    The Electron Transport Chain (ETC) is a series of protein complexes embedded in the inner mitochondrial membrane. Electrons are passed from one complex to another, releasing energy that is used to pump protons across the membrane, creating a proton gradient.

    How do NADH and FADH2 play a role in the ETC?

    NADH and FADH2 are electron carriers that deliver electrons to the ETC. NADH enters at Complex I, while FADH2 enters at Complex II.

    How does the ETC contribute to ATP synthesis?

    The ETC pumps protons across the inner mitochondrial membrane, creating a proton gradient. This gradient drives the synthesis of ATP by ATP synthase, a protein complex that uses the energy of proton flow to create ATP.

    Explain glycolysis in the context of ATP production.

    Glycolysis is a metabolic pathway occurring in the cytoplasm that breaks down glucose into pyruvate, generating ATP and NADH.

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    What is the Citric Acid Cycle (TCA cycle) and its role in ATP production?

    The Citric Acid Cycle (TCA cycle) takes place in the mitochondrial matrix and further oxidizes pyruvate, generating ATP, NADH, and FADH2.

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    Explain the malate-aspartate shuttle.

    The malate-aspartate shuttle transfers electrons from NADH in the cytoplasm to NADH in the mitochondria. This process allows for continued ATP production from glycolysis.

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    What is the glycerol 3-phosphate shuttle?

    The glycerol 3-phosphate shuttle transfers electrons from NADH in the cytoplasm to FADH2 in the mitochondria. This shuttle is less efficient than the malate-aspartate shuttle, resulting in less ATP production.

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    What is glycogen and its role in energy storage?

    Glycogen is a stored form of glucose found primarily in the liver and muscles. It serves as a readily available energy source when blood glucose levels drop.

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    What is gluconeogenesis?

    Gluconeogenesis is a metabolic pathway that synthesizes glucose from non-carbohydrate sources like amino acids and glycerol. It helps maintain blood glucose levels when dietary intake is low.

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    What is the Pentose Phosphate Pathway (PPP) and its functions?

    The Pentose Phosphate Pathway (PPP) is an alternative pathway of glucose metabolism that generates NADPH (a reducing agent) and ribose-5-phosphate (a precursor for nucleotide synthesis).

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    Bioenergetics

    The study of energy transfer and utilization within living organisms. It explains how cells obtain, store, and use energy for essential processes.

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    Free Energy Change (ΔG)

    A measure of the energy change in a reaction. A negative ΔG indicates a spontaneous reaction, releasing energy (exergonic). A positive ΔG indicates a non-spontaneous reaction, requiring energy input (endergonic).

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    ATP (Adenosine Triphosphate)

    The energy currency of cells. It stores and releases energy through the breaking and forming of its phosphate bonds.

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    Substrate-Level Phosphorylation

    The direct transfer of a phosphate group from a substrate molecule to ADP, generating ATP. This occurs in glycolysis and the citric acid cycle.

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    Oxidative Phosphorylation

    The production of ATP using energy released from the electron transport chain. This is the main ATP production pathway in aerobic respiration.

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    Glycolysis

    A metabolic pathway that breaks down glucose into pyruvate, generating ATP and NADH. It occurs in the cytoplasm of cells.

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    Gluconeogenesis

    A metabolic pathway that synthesizes glucose from non-carbohydrate precursors, such as pyruvate, lactate, and glycerol. It occurs primarily in the liver.

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    Pentose Phosphate Pathway

    A metabolic pathway that generates NADPH and ribose 5-phosphate, essential for nucleotide synthesis and detoxification. It occurs in the cytoplasm of cells.

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    Glycogenolysis

    The breakdown of glycogen, a stored form of glucose, into glucose molecules. This is the process of releasing glucose when needed.

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    Glycogenesis

    The synthesis of glycogen from glucose molecules. This is the process of storing glucose for later use.

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    Citric Acid Cycle (TCA Cycle)

    A series of reactions that oxidize acetyl-CoA, generating electron carriers (NADH and FADH2) and precursors for biosynthesis. It occurs in the mitochondria of cells.

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    Electron Transport Chain (ETC)

    The process where electrons from NADH and FADH2 are passed through a chain of protein complexes, releasing energy to pump protons across the mitochondrial membrane. This proton gradient drives ATP synthesis.

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    Oxidative Phosphorylation

    The process of transferring electrons from NADH and FADH2 to oxygen, ultimately generating water. This is the final step in aerobic respiration.

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    Fermentation

    A metabolic pathway that regenerates NAD+ from NADH under anaerobic conditions. It produces lactate (lactic acid fermentation) or ethanol (alcoholic fermentation).

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    Pyruvate Dehydrogenase Complex (PDC)

    A metabolic pathway that occurs in the mitochondria. It breaks down pyruvate into acetyl-CoA, which enters the citric acid cycle.

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    Pyruvate Transport

    The process of moving pyruvate from the cytoplasm into mitochondria. This is essential for energy production in aerobic respiration.

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    What is glycogen?

    A branched homopolysaccharide composed of α-D-glucose monomers. The main chain is linked by α-(1,4) glycosidic bonds, while branches are formed by α-(1,6) glycosidic bonds. A protein called glycogenin serves as the core of the glycogen molecule.

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    What is the function of glycogen in skeletal muscle?

    Glycogen stored in skeletal muscle is primarily used for the muscle's own energy needs, particularly during periods of intense activity. It allows for quick, local energy delivery to fuel muscle contractions.

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    What is the function of glycogen in the liver?

    Liver glycogen serves as a glucose reservoir for the whole body. It plays a crucial role in regulating blood glucose levels, ensuring a steady supply of glucose for the brain and other tissues.

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    What is glycogen synthesis?

    The process of converting glucose into glycogen for storage. It involves the activation of glucose 1-phosphate by UDP (uridine diphosphate) to form UDP-glucose, which is then added to the growing glycogen chain.

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    What is glycogen degradation?

    The breakdown of glycogen into glucose for energy production. It involves the cleavage of glucose 1-phosphate molecules from the ends of glycogen chains. One molecule of free glucose is also released from the branching point.

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    What is glycolysis?

    A series of metabolic reactions that convert glucose into pyruvate (or lactate) for energy production. It is the primary pathway for glucose metabolism.

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    What is the pentose phosphate pathway?

    A metabolic pathway that converts glucose into ribose 5-phosphate, a precursor for nucleotide biosynthesis, and NADPH, a reducing agent important for various metabolic processes.

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    Study Notes

    Bioenergetics

    • Bioenergetics is the transfer and utilization of energy in biological systems
    • Bioenergetics predicts if a process is possible, whereas kinetics measures the reaction rate
    • Enzymes cannot make a reaction happen on their own
    • Energy difference (ΔG) = (Final E) - (Initial E). A negative ΔG indicates a spontaneous, exergonic reaction. A positive ΔG indicates a nonspontaneous, endergonic reaction.
    • The change in free energy of a reaction (ΔG) is determined by the difference in energy between the products and reactants.
    • Enzymes don't change ΔG, they lower the activation energy needed for a reaction to occur

    ATP Production

    • ATP production occurs through two main pathways:
      • Substrate-level phosphorylation: Direct transfer of a phosphate group from a phosphorylated intermediate to ADP to form ATP (e.g., in glycolysis)
      • Oxidative phosphorylation: Production of ATP by the electron transport chain (ETC), fueled by electrons from NADH and FADH2 (e.g., in mitochondria)

    Carbohydrate Metabolism

    • Carbohydrates are the primary source of energy for the body.
    • Glycolysis is the breakdown of glucose into pyruvate, producing ATP and NADH.
    • Glycogenesis is the synthesis of glycogen from glucose.
    • Glycogenolysis is the breakdown of glycogen into glucose.
    • Gluconeogenesis is the synthesis of glucose from non-carbohydrate precursors (like amino acids).
    • The pentose phosphate pathway produces NADPH and ribose 5-phosphate.

    TCA Cycle

    • The TCA cycle (also known as the citric acid cycle) is an important metabolic pathway in the mitochondria.
    • It plays an essential role in energy production. Pyruvate, produced from glycolysis, is converted to Acetyl-CoA to enter the TCA cycle.
    • The TCA cycle generates NADH and FADH2 (electron carriers) and produces CO₂.
    • Intermediates from the TCA cycle can be used to synthesize various biomolecules, demonstrating its biosynthetic role

    Oxidative Phosphorylation and ETC

    • The electron transport chain (ETC) is a series of protein complexes in the inner mitochondrial membrane.
    • NADH and FADH₂ (produced from glycolysis and TCA cycle) deliver electrons to the ETC.
    • The energy released from electron transfer through the ETC is used to pump protons (H+) across the membrane creating an electrochemical gradient.
    • ATP synthase uses this gradient to synthesize ATP.

    Pentose Phosphate Pathway

    • The pentose phosphate pathway is an alternative metabolic pathway for glucose.
    • It produces NADPH, essential for reducing agents in biosynthetic processes, and ribose 5-phosphate required for nucleotide synthesis and nucleic acid synthesis.
    • The oxidative reactions of this pathway are irreversible
    • The nonoxidative reactions are reversible.

    Glycogen

    • Storage form of glucose in animals
    • Found in liver and muscle tissue
    • Branched structure that provides multiple points for breakdown when needed
    • The branching allows for rapid glucose release and storage when energy is demanded or excess fuel is available for storage

    Hormonal Regulation of Glycolysis

    • Insulin stimulates glycolysis (used to increase glucose from blood).
    • Glucagon inhibits glycolysis (used to decrease glucose from blood).

    Glycolysis: NAD+ Regeneration

    • NAD+ serves as an electron acceptor during glycolysis. When O2 is present, NADH produced by glycolysis can be oxidized via oxidative phosphorylation in the mitochondria.
    • When O2 is absent, fermentation pathways (e.g., lactate production) regenerate NAD+ in the cytoplasm allowing anaerobic respiration to proceed.

    Malate Aspartate Shuttle

    • A mechanism to transfer electrons from NADH in the cytosol to the mitochondrial matrix.
    • Through a series of reactions, NADH from glycolysis in the cytoplasm is converted to NADH within the mitochondrial matrix, allowing for its use in oxidative phosphorylation.

    Glycerol 3-Phosphate Shuttle

    • Another method that transfers high energy electrons from the cytoplasm to the mitochondria.
    • Conversion of NADH in the cytoplasm to FADH2 in the mitochondria to support oxidative phosphorylation without transferring NADH directly

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    Description

    Explore the fundamentals of bioenergetics and the pathways of ATP production. This quiz covers concepts such as energy transfer in biological systems, the role of enzymes, and the processes of substrate-level and oxidative phosphorylation. Test your understanding of energy changes in biochemical reactions!

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