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Explain the function of enzymes in biochemical reactions.
Enzymes are proteins that act as biological catalysts, accelerating biochemical reactions in the human body. They have various functions such as aiding in digestion and eliminating toxins.
What are the two identified sites within enzymes and their functions?
The two identified sites within enzymes are the catalytic site, which is involved in catalysis, and the substrate binding site, where reactants called substrates bind to the enzyme.
Explain the clinical significance of enzymes in the blood serum.
If enzymes are found in high quantity in the blood serum, it indicates tissue damage, giving enzymes diagnostic value. Enzymes can also be used as drugs.
List three important properties of enzymes.
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What is the function of Oxidoreductase enzymes?
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What are the important properties of enzymes? Provide at least three properties.
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Explain the function of enzymes in the human body and provide an example of their role in bodily processes.
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What is the active site of an enzyme and what is its function?
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Explain the clinical significance of finding high quantities of enzymes in the blood serum.
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List and explain the function of at least three classifications of enzymes.
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Explain the function of Hydrolases enzymes and provide an example of a biochemical reaction they catalyze?
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What is the clinical significance of finding high quantities of enzymes in the blood serum? Provide an example of an enzyme used for diagnostic purposes.
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Explain the properties of enzymes that differentiate them from non-biochemical catalysts. Provide at least two properties.
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What are the two identified sites within enzymes and their functions?
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What is the function of Transferases enzymes and provide an example of a molecular group transfer reaction they catalyze?
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What is the primary function of Oxidoreductase enzymes?
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Which type of enzyme catalyzes the transfer of a molecular group from one molecule to another?
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What distinguishes enzymes from non-biochemical catalysts?
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What is the clinical significance of finding high quantities of enzymes in the blood serum?
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What is the primary property of enzymes related to their catalytic function?
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Study Notes
Enzymes in Biochemical Reactions
- Enzymes are biological molecules that catalyze biochemical reactions, increasing their rates and efficiency without being consumed or altered in the process.
- Enzymes have two identified sites: the active site and the binding site. The active site is where the substrate binds, and the enzyme catalyzes the reaction. The binding site is where the enzyme recognizes and binds to the substrate.
Properties of Enzymes
- Enzymes are highly specific, meaning they only catalyze one specific reaction or a small group of related reactions.
- Enzymes are highly efficient, allowing them to catalyze reactions at a much faster rate than non-biochemical catalysts.
- Enzymes are highly regulated, meaning their activity can be controlled and adjusted according to the needs of the cell.
Clinical Significance of Enzymes in Blood Serum
- High quantities of enzymes in the blood serum can indicate tissue damage or disease, making enzymes important diagnostic tools.
- Elevated levels of certain enzymes can be indicative of specific diseases, such as liver or pancreatic disease.
Classifications of Enzymes
- Oxidoreductases: catalyze oxidation and reduction reactions, often involving the transfer of electrons.
- Transferases: catalyze the transfer of a molecular group from one molecule to another.
- Hydrolases: catalyze the hydrolysis of a molecule, involving the breaking of a chemical bond using water.
Examples of Enzymes
- Hydrolases: peptidases, which break down peptides into amino acids.
- Transferases: transaminases, which catalyze the transfer of an amino group from one molecule to another.
- Oxidoreductases: cytochrome P450, which catalyzes the oxidation of xenobiotics in the liver.
Active Site and its Function
- The active site is a region on the enzyme where the substrate binds, and the enzyme catalyzes the reaction.
- The active site is highly specific, allowing the enzyme to recognize and bind to the correct substrate.
Diagnostic Use of Enzymes
- Enzymes can be used as diagnostic tools to detect specific diseases or conditions.
- For example, lactate dehydrogenase (LDH) is an enzyme used to diagnose tissue damage or disease.
Differentiation from Non-Biochemical Catalysts
- Enzymes are highly specific, whereas non-biochemical catalysts are often non-specific and can catalyze a wide range of reactions.
- Enzymes are highly regulated, whereas non-biochemical catalysts are often unregulated and can catalyze reactions at a constant rate.
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Description
Test your knowledge on the biochemistry of enzymes, including their functions and mechanisms of action. Explore the fundamental role of enzymes as biological catalysts in various biochemical reactions within the human body.