Podcast
Questions and Answers
What is the end product of glycolysis in anaerobic conditions?
What is the end product of glycolysis in anaerobic conditions?
- Lactate (correct)
- Acetyl-CoA
- Pyruvate
- Carbon dioxide
Which pathway produces ribose and reducing equivalents from glycolysis intermediates?
Which pathway produces ribose and reducing equivalents from glycolysis intermediates?
- Fatty acid synthesis
- Pentose phosphate pathway (correct)
- Glycogenolysis
- Citric acid cycle
What component is required for synthesizing long-chain fatty acids?
What component is required for synthesizing long-chain fatty acids?
- Acetyl-CoA (correct)
- Ribose
- Glycerol
- Amino acids
During β-oxidation, what is acetyl-CoA primarily oxidized to?
During β-oxidation, what is acetyl-CoA primarily oxidized to?
What is the main caloric reserve of the body formed from fatty acids?
What is the main caloric reserve of the body formed from fatty acids?
Which substance is formed in the liver from acetyl-CoA and serves as a precursor for ketone bodies?
Which substance is formed in the liver from acetyl-CoA and serves as a precursor for ketone bodies?
Which type of amino acids must be supplied specifically in the diet?
Which type of amino acids must be supplied specifically in the diet?
What process allows tissues to utilize oxygen for complete metabolism of pyruvate?
What process allows tissues to utilize oxygen for complete metabolism of pyruvate?
What is the main purpose of catabolism?
What is the main purpose of catabolism?
Which stage of catabolism involves the conversion of complex molecules to their building blocks?
Which stage of catabolism involves the conversion of complex molecules to their building blocks?
During which stage of catabolism is a small amount of energy captured as ATP?
During which stage of catabolism is a small amount of energy captured as ATP?
What is the primary metabolic function of the liver concerning metabolites?
What is the primary metabolic function of the liver concerning metabolites?
Which metabolic pathway is involved in the final oxidation of energy-rich molecules?
Which metabolic pathway is involved in the final oxidation of energy-rich molecules?
After deamination of amino acids, what compound is formed from excess amino nitrogen?
After deamination of amino acids, what compound is formed from excess amino nitrogen?
What is the primary reaction type that occurs during anabolism?
What is the primary reaction type that occurs during anabolism?
Which of the following intermediates is NOT a starting material for anabolic reactions?
Which of the following intermediates is NOT a starting material for anabolic reactions?
Which of the following statements about glycolysis is true?
Which of the following statements about glycolysis is true?
Which metabolites share a common route of absorption via the hepatic portal vein?
Which metabolites share a common route of absorption via the hepatic portal vein?
What is necessary for anabolic reactions besides the availability of precursors?
What is necessary for anabolic reactions besides the availability of precursors?
Which of the following statements about metabolites is true?
Which of the following statements about metabolites is true?
What result occurs from the glycolytic breakdown of glucose in certain mammalian tissues?
What result occurs from the glycolytic breakdown of glucose in certain mammalian tissues?
What happens to excess glucose during the metabolic process in the liver?
What happens to excess glucose during the metabolic process in the liver?
Where does glycolysis primarily take place within a cell?
Where does glycolysis primarily take place within a cell?
What process allows for the conversion of carbon skeletons remaining after transamination?
What process allows for the conversion of carbon skeletons remaining after transamination?
What is the role of ATP in the first step of glycolysis?
What is the role of ATP in the first step of glycolysis?
Which enzyme catalyzes the isomerization of glucose 6-phosphate to fructose 6-phosphate?
Which enzyme catalyzes the isomerization of glucose 6-phosphate to fructose 6-phosphate?
What compound is produced from the cleavage of fructose 1,6-bisphosphate?
What compound is produced from the cleavage of fructose 1,6-bisphosphate?
In the pay-off phase of glycolysis, what is generated alongside ATP during the transfer from 1,3-bisphosphoglycerate?
In the pay-off phase of glycolysis, what is generated alongside ATP during the transfer from 1,3-bisphosphoglycerate?
What is the end product of glycolysis derived from glucose?
What is the end product of glycolysis derived from glucose?
During which step of glycolysis does the first oxidation of glyceraldehyde 3-phosphate occur?
During which step of glycolysis does the first oxidation of glyceraldehyde 3-phosphate occur?
Which step of glycolysis involves the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate?
Which step of glycolysis involves the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate?
What type of reaction does aldolase catalyze during glycolysis?
What type of reaction does aldolase catalyze during glycolysis?
Glycolysis can only occur in the presence of oxygen.
Glycolysis can only occur in the presence of oxygen.
Acetyl-CoA can be formed from the metabolism of fatty acids.
Acetyl-CoA can be formed from the metabolism of fatty acids.
The pentose phosphate pathway provides ribose and reducing equivalents for the synthesis of nucleotides.
The pentose phosphate pathway provides ribose and reducing equivalents for the synthesis of nucleotides.
Pyruvate cannot be converted to acetyl-CoA in aerobic tissues.
Pyruvate cannot be converted to acetyl-CoA in aerobic tissues.
Glycogen is predominantly stored in the liver and skeletal muscle as a source of glucose.
Glycogen is predominantly stored in the liver and skeletal muscle as a source of glucose.
Fatty acid synthesis can only occur using lipid sources from the diet.
Fatty acid synthesis can only occur using lipid sources from the diet.
Amino acids are essential for protein synthesis and can be synthesized by the body.
Amino acids are essential for protein synthesis and can be synthesized by the body.
Acetyl-CoA can be used to synthesize cholesterol in the liver.
Acetyl-CoA can be used to synthesize cholesterol in the liver.
Amino acids are only required for protein synthesis in the body.
Amino acids are only required for protein synthesis in the body.
Glycolysis occurs exclusively in the mitochondria of the cell.
Glycolysis occurs exclusively in the mitochondria of the cell.
Catabolism involves the formation of complex molecules from simpler precursors.
Catabolism involves the formation of complex molecules from simpler precursors.
Acetyl-CoA is completely oxidized to CO2 in the final stage of catabolism.
Acetyl-CoA is completely oxidized to CO2 in the final stage of catabolism.
Liver plays a key role in regulating blood glucose concentrations by converting excess glucose to glycogen.
Liver plays a key role in regulating blood glucose concentrations by converting excess glucose to glycogen.
Anabolism is a process that only occurs in the presence of reducing equivalents like NADH.
Anabolism is a process that only occurs in the presence of reducing equivalents like NADH.
The glycolytic breakdown of glucose can be the sole source of metabolic energy for certain mammalian tissues.
The glycolytic breakdown of glucose can be the sole source of metabolic energy for certain mammalian tissues.
During gluconeogenesis, carbon skeletons can be formed from amino acids.
During gluconeogenesis, carbon skeletons can be formed from amino acids.
The Krebs cycle accepts carbon compounds derived from carbohydrates, lipids, or proteins.
The Krebs cycle accepts carbon compounds derived from carbohydrates, lipids, or proteins.
Excess amino nitrogen is eliminated through ammonium production in the body.
Excess amino nitrogen is eliminated through ammonium production in the body.
Metabolites can only refer to products of metabolic reactions.
Metabolites can only refer to products of metabolic reactions.
Stage II of catabolism involves the complete degradation of building blocks to simple intermediates.
Stage II of catabolism involves the complete degradation of building blocks to simple intermediates.
Metabolites absorbed via the hepatic portal vein are initially directed to the lungs.
Metabolites absorbed via the hepatic portal vein are initially directed to the lungs.
Many anaerobic microorganisms rely solely on the process of oxidative phosphorylation for energy.
Many anaerobic microorganisms rely solely on the process of oxidative phosphorylation for energy.
Catabolic reactions capture energy in the form of ATP during the breakdown of biomolecules.
Catabolic reactions capture energy in the form of ATP during the breakdown of biomolecules.
Biosynthetic reactions in anabolism depend solely on the availability of ATP.
Biosynthetic reactions in anabolism depend solely on the availability of ATP.
Glycolysis consists of eight steps in total.
Glycolysis consists of eight steps in total.
Fructose 1,6-bisphosphate is cleaved into two triose phosphates.
Fructose 1,6-bisphosphate is cleaved into two triose phosphates.
The first step of glycolysis involves the transfer of a phosphoryl group from ADP to glucose.
The first step of glycolysis involves the transfer of a phosphoryl group from ADP to glucose.
The enzyme phosphoglycerate kinase is responsible for the oxidation of glyceraldehyde 3-phosphate.
The enzyme phosphoglycerate kinase is responsible for the oxidation of glyceraldehyde 3-phosphate.
One molecule of glucose yields two molecules of glyceraldehyde 3-phosphate in glycolysis.
One molecule of glucose yields two molecules of glyceraldehyde 3-phosphate in glycolysis.
The pay-off phase of glycolysis conserves free energy in the form of NADH.
The pay-off phase of glycolysis conserves free energy in the form of NADH.
Phosphofructokinase-1 catalyzes the isomerization of glucose 6-phosphate to fructose 6-phosphate.
Phosphofructokinase-1 catalyzes the isomerization of glucose 6-phosphate to fructose 6-phosphate.
Dihydroxyacetone phosphate is an aldose sugar.
Dihydroxyacetone phosphate is an aldose sugar.
What is the primary purpose of catabolism in metabolic processes?
What is the primary purpose of catabolism in metabolic processes?
During which stage of catabolism is pyruvate formed from building blocks?
During which stage of catabolism is pyruvate formed from building blocks?
What role does acetyl-CoA play in the metabolic processes?
What role does acetyl-CoA play in the metabolic processes?
Which of the following compounds can serve as starting materials for anabolic reactions?
Which of the following compounds can serve as starting materials for anabolic reactions?
What is a significant function of the Krebs cycle in cellular metabolism?
What is a significant function of the Krebs cycle in cellular metabolism?
Which of the following statements about anabolic reactions is incorrect?
Which of the following statements about anabolic reactions is incorrect?
Which process is involved in the transformation of simple intermediates into building blocks?
Which process is involved in the transformation of simple intermediates into building blocks?
What is formed from the carbon skeletons that remain after transamination?
What is formed from the carbon skeletons that remain after transamination?
Which amino acids are primarily supplied in the diet?
Which amino acids are primarily supplied in the diet?
What occurs during the final oxidation of acetyl-CoA in metabolic pathways?
What occurs during the final oxidation of acetyl-CoA in metabolic pathways?
What metabolic function does the liver perform with excess glucose?
What metabolic function does the liver perform with excess glucose?
Which compound is a product of glycolysis?
Which compound is a product of glycolysis?
What happens to amino nitrogen after deamination in the body?
What happens to amino nitrogen after deamination in the body?
Where does glycolysis occur within a cell?
Where does glycolysis occur within a cell?
What is the primary function of acetyl-CoA derived from β-oxidation?
What is the primary function of acetyl-CoA derived from β-oxidation?
Which type of tissues rely solely on glycolysis for metabolic energy?
Which type of tissues rely solely on glycolysis for metabolic energy?
What is a key role of the pentose phosphate pathway?
What is a key role of the pentose phosphate pathway?
During starvation, what becomes an important source of energy?
During starvation, what becomes an important source of energy?
Which of the following molecules can be derived from the intermediates of glycolysis?
Which of the following molecules can be derived from the intermediates of glycolysis?
What is the significance of the conversion of pyruvate to acetyl-CoA?
What is the significance of the conversion of pyruvate to acetyl-CoA?
What is a primary source for fatty acid synthesis?
What is a primary source for fatty acid synthesis?
In which organ is acetoacetate formed, and what is its primary function?
In which organ is acetoacetate formed, and what is its primary function?
Which molecule is primarily generated during the rapid phase of glycolysis?
Which molecule is primarily generated during the rapid phase of glycolysis?
What is the main purpose of glycogen in the body?
What is the main purpose of glycogen in the body?
What is the first product formed from the phosphorylation of glucose in glycolysis?
What is the first product formed from the phosphorylation of glucose in glycolysis?
Which enzyme catalyzes the conversion of glucose 6-phosphate to fructose 6-phosphate?
Which enzyme catalyzes the conversion of glucose 6-phosphate to fructose 6-phosphate?
In the pay-off phase of glycolysis, what are the two molecules primarily formed from one glucose molecule?
In the pay-off phase of glycolysis, what are the two molecules primarily formed from one glucose molecule?
What type of reaction occurs during Step 4 of glycolysis when fructose 1,6-bisphosphate is cleaved?
What type of reaction occurs during Step 4 of glycolysis when fructose 1,6-bisphosphate is cleaved?
During the oxidation of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, what is released?
During the oxidation of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, what is released?
Which enzyme is responsible for transferring a phosphoryl group from 1,3-bisphosphoglycerate to ADP?
Which enzyme is responsible for transferring a phosphoryl group from 1,3-bisphosphoglycerate to ADP?
What is the purpose of the preparatory phase in glycolysis?
What is the purpose of the preparatory phase in glycolysis?
Which of the following accurately describes the inter-conversion of triose phosphates in glycolysis?
Which of the following accurately describes the inter-conversion of triose phosphates in glycolysis?
Flashcards
What is metabolism?
What is metabolism?
The sum of all chemical reactions that occur within a living organism.
What is a metabolic pathway?
What is a metabolic pathway?
A series of enzyme-catalyzed steps that produce a specific product.
What is catabolism?
What is catabolism?
The process of breaking down complex molecules into simpler ones, releasing energy.
What is anabolism?
What is anabolism?
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What is a metabolite?
What is a metabolite?
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What is ATP?
What is ATP?
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What happens in Stage I of Catabolism?
What happens in Stage I of Catabolism?
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What happens in Stage II of Catabolism?
What happens in Stage II of Catabolism?
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Glycolysis
Glycolysis
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Aerobic Metabolism
Aerobic Metabolism
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Glycogen
Glycogen
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Pentose Phosphate Pathway
Pentose Phosphate Pathway
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β-oxidation
β-oxidation
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Triacylglycerol (Fat)
Triacylglycerol (Fat)
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Ketone Bodies
Ketone Bodies
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Essential Amino Acids
Essential Amino Acids
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Non-essential amino acids
Non-essential amino acids
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Transamination
Transamination
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Deamination
Deamination
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Liver's role in metabolism
Liver's role in metabolism
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Glycogenesis
Glycogenesis
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Glycogenolysis
Glycogenolysis
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Lipogenesis
Lipogenesis
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What is the first step in glycolysis?
What is the first step in glycolysis?
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How is glucose 6-phosphate converted to fructose 6-phosphate?
How is glucose 6-phosphate converted to fructose 6-phosphate?
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What happens in the second phosphorylation step of glycolysis?
What happens in the second phosphorylation step of glycolysis?
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What happens to fructose 1,6-bisphosphate?
What happens to fructose 1,6-bisphosphate?
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What is the purpose of the inter-conversion of the triose phosphates?
What is the purpose of the inter-conversion of the triose phosphates?
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What happens during the oxidation of glyceraldehyde 3-phosphate?
What happens during the oxidation of glyceraldehyde 3-phosphate?
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How is ATP generated in the pay-off phase of glycolysis?
How is ATP generated in the pay-off phase of glycolysis?
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What is the pay-off phase in glycolysis?
What is the pay-off phase in glycolysis?
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Study Notes
Biochemistry Module: Introduction to Metabolism & Carbohydrate Metabolism
- The module covers biochemistry, metabolism, and carbohydrate metabolism.
- The lecturer is Dr Mohammed Mansour, a Senior Lecturer in Biomedical Science at London South Bank University.
- The module's duration is from 12:00 to 15:00.
- Online access via VLE is used.
- A one-time code for access is SO-HB-BK.
Catabolic and Anabolic Reactions
- Catabolism is a degradative process that breaks down complex molecules into simpler ones, releasing energy.
- Anabolism is a biosynthetic process building complex molecules from simpler ones, requiring energy.
- Complex organic molecules like carbohydrates, lipids, and proteins are broken down into simpler molecules like simple sugars, fatty acids, and amino acids.
- The released energy is in the form of ATP (adenosine triphosphate) and NADPH.
Introduction to Metabolism
- Metabolism encompasses all chemical reactions within a living system.
- A metabolic pathway is a series of enzymatic reactions that produce desired products.
- Metabolites are substrates, intermediates, or products in metabolic reactions.
- Metabolism is classified into catabolism and anabolism.
- Enzymes act as biological catalysts in these reactions.
Outline of Metabolism
- Stage I of catabolism: conversion of complex molecules into their building blocks. (e.g., polysaccharides to monosaccharides, lipids to fatty acids and glycerol, proteins to amino acids).
- Stage II of catabolism: formation of simple intermediates (e.g., pyruvate and acetyl-CoA).
- Stage III of catabolism: final oxidation of acetyl-CoA (e.g., Krebs cycle).
Carbohydrate Metabolism
- Carbohydrate metabolism focuses on glucose's fate, including glycolysis.
- Glycolysis converts glucose to pyruvate and lactate in all cells.
- Anaerobic conditions result in only lactate as a product.
- Aerobic tissues utilize oxygen, converting pyruvate to acetyl-CoA, enabling entry into the citric acid cycle for complete oxidation into CO2 and H2O, releasing ATP.
- Glucose is a major fuel source for many tissues and is involved in various processes.
Other Metabolic Pathways
- Glycogen synthesis: Glucose is converted into glycogen, primarily in liver and muscle tissue.
- Pentose phosphate pathway: A source of reducing equivalents (2H) for biosynthesis (e.g., fatty acids), and a source of ribose for nucleotides and nucleic acid formation.
- Fatty acid synthesis/biosynthesis: Acetyl-CoA is the source for fatty acid synthesis. Fatty acids are either derived from dietary components or through de novo synthesis by acetyl-CoA.
- Fatty acid oxidation: Fatty acids' breakdown into acetyl-CoA (β-oxidation) and incorporation into triacylglycerol.
- Amino acid metabolism: Necessary for protein synthesis. Some amino acids are essential (dietary) and others are non-essential (formed from intermediates). Transamination and deamination are key processes.
- Ketone bodies: The liver produces ketone bodies, a source of alternative water-soluble metabolic fuel during conditions like starvation.
Metabolic Pathways - Levels of Organization
- Metabolic pathways are studied at different levels:
- Subcellular level: organelles like mitochondria and cytosol have specific roles and a pattern.
- Tissue and organ level: the nature of substrates entering and metabolites leaving tissues and organs are observed.
- Blood circulation integrates metabolism, carrying amino acids and glucose from digestion to other body parts.
Liver's Role in Metabolism
- The liver plays a primary role in regulating metabolite levels, particularly for glucose.
- Glucose homeostasis is regulated through glycogen storage (glycogenesis) or conversion to fat (lipogenesis).
- Between meals, the liver uses stored glycogen for glucose release.
Glycolysis
- Glycolysis is a universal central pathway of glucose breakdown in most cells.
- In some cells (erythrocytes, renal medulla, brain, and sperm), it is the sole metabolic energy source.
- It occurs in the cytoplasm.
- The process breaks down glucose into pyruvate, with energy conservation in ATP form.
- Steps involved include phosphorylation phases and energy-conservation phases.
- In anaerobic conditions, pyruvate is reduced to lactate, regenerating NAD+.
Additional Notes
- The provided slides provide an outline of the biochemistry modules' content, concentrating on metabolism and carbohydrate metabolism.
- A variety of reactions, processes, and molecules are discussed.
- Key concepts like catabolism, anabolism, glycolysis, and enzymes are highlighted.
- The importance of different organs like the liver in managing metabolic processes is emphasized.
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