Biochemistry Module: Metabolism Overview

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Questions and Answers

What is the end product of glycolysis in anaerobic conditions?

  • Lactate (correct)
  • Acetyl-CoA
  • Pyruvate
  • Carbon dioxide

Which pathway produces ribose and reducing equivalents from glycolysis intermediates?

  • Fatty acid synthesis
  • Pentose phosphate pathway (correct)
  • Glycogenolysis
  • Citric acid cycle

What component is required for synthesizing long-chain fatty acids?

  • Acetyl-CoA (correct)
  • Ribose
  • Glycerol
  • Amino acids

During β-oxidation, what is acetyl-CoA primarily oxidized to?

<p>CO2 and H2O (A)</p> Signup and view all the answers

What is the main caloric reserve of the body formed from fatty acids?

<p>Acylglycerol (C)</p> Signup and view all the answers

Which substance is formed in the liver from acetyl-CoA and serves as a precursor for ketone bodies?

<p>Acetoacetate (B)</p> Signup and view all the answers

Which type of amino acids must be supplied specifically in the diet?

<p>Essential amino acids (B)</p> Signup and view all the answers

What process allows tissues to utilize oxygen for complete metabolism of pyruvate?

<p>Aerobic respiration (B)</p> Signup and view all the answers

What is the main purpose of catabolism?

<p>To break down molecules and generate energy (D)</p> Signup and view all the answers

Which stage of catabolism involves the conversion of complex molecules to their building blocks?

<p>Stage I (C)</p> Signup and view all the answers

During which stage of catabolism is a small amount of energy captured as ATP?

<p>Stage II (B)</p> Signup and view all the answers

What is the primary metabolic function of the liver concerning metabolites?

<p>Regulation of blood concentration of metabolites (D)</p> Signup and view all the answers

Which metabolic pathway is involved in the final oxidation of energy-rich molecules?

<p>Krebs's cycle (B)</p> Signup and view all the answers

After deamination of amino acids, what compound is formed from excess amino nitrogen?

<p>Urea (D)</p> Signup and view all the answers

What is the primary reaction type that occurs during anabolism?

<p>Biosynthetic reactions (C)</p> Signup and view all the answers

Which of the following intermediates is NOT a starting material for anabolic reactions?

<p>NADPH (D)</p> Signup and view all the answers

Which of the following statements about glycolysis is true?

<p>Glycolysis is a pathway of glucose catabolism (A)</p> Signup and view all the answers

Which metabolites share a common route of absorption via the hepatic portal vein?

<p>Amino acids and glucose (D)</p> Signup and view all the answers

What is necessary for anabolic reactions besides the availability of precursors?

<p>Energy (ATP or GTP) (A)</p> Signup and view all the answers

Which of the following statements about metabolites is true?

<p>Metabolites can be substrates or products in metabolic reactions (D)</p> Signup and view all the answers

What result occurs from the glycolytic breakdown of glucose in certain mammalian tissues?

<p>It serves as the sole source of metabolic energy (B)</p> Signup and view all the answers

What happens to excess glucose during the metabolic process in the liver?

<p>It is stored as glycogen or fat (C)</p> Signup and view all the answers

Where does glycolysis primarily take place within a cell?

<p>Cytoplasm (C)</p> Signup and view all the answers

What process allows for the conversion of carbon skeletons remaining after transamination?

<p>Gluconeogenesis and ketogenesis (B)</p> Signup and view all the answers

What is the role of ATP in the first step of glycolysis?

<p>It donates a phosphoryl group to glucose. (B)</p> Signup and view all the answers

Which enzyme catalyzes the isomerization of glucose 6-phosphate to fructose 6-phosphate?

<p>Phosphohexose isomerase (C)</p> Signup and view all the answers

What compound is produced from the cleavage of fructose 1,6-bisphosphate?

<p>Two triose phosphates (D)</p> Signup and view all the answers

In the pay-off phase of glycolysis, what is generated alongside ATP during the transfer from 1,3-bisphosphoglycerate?

<p>3-phosphoglycerate (D)</p> Signup and view all the answers

What is the end product of glycolysis derived from glucose?

<p>Pyruvate (D)</p> Signup and view all the answers

During which step of glycolysis does the first oxidation of glyceraldehyde 3-phosphate occur?

<p>Step 6 (A)</p> Signup and view all the answers

Which step of glycolysis involves the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate?

<p>Step 3 (B)</p> Signup and view all the answers

What type of reaction does aldolase catalyze during glycolysis?

<p>Aldol condensation (D)</p> Signup and view all the answers

Glycolysis can only occur in the presence of oxygen.

<p>False (B)</p> Signup and view all the answers

Acetyl-CoA can be formed from the metabolism of fatty acids.

<p>True (A)</p> Signup and view all the answers

The pentose phosphate pathway provides ribose and reducing equivalents for the synthesis of nucleotides.

<p>True (A)</p> Signup and view all the answers

Pyruvate cannot be converted to acetyl-CoA in aerobic tissues.

<p>False (B)</p> Signup and view all the answers

Glycogen is predominantly stored in the liver and skeletal muscle as a source of glucose.

<p>True (A)</p> Signup and view all the answers

Fatty acid synthesis can only occur using lipid sources from the diet.

<p>False (B)</p> Signup and view all the answers

Amino acids are essential for protein synthesis and can be synthesized by the body.

<p>False (B)</p> Signup and view all the answers

Acetyl-CoA can be used to synthesize cholesterol in the liver.

<p>True (A)</p> Signup and view all the answers

Amino acids are only required for protein synthesis in the body.

<p>False (B)</p> Signup and view all the answers

Glycolysis occurs exclusively in the mitochondria of the cell.

<p>False (B)</p> Signup and view all the answers

Catabolism involves the formation of complex molecules from simpler precursors.

<p>False (B)</p> Signup and view all the answers

Acetyl-CoA is completely oxidized to CO2 in the final stage of catabolism.

<p>True (A)</p> Signup and view all the answers

Liver plays a key role in regulating blood glucose concentrations by converting excess glucose to glycogen.

<p>True (A)</p> Signup and view all the answers

Anabolism is a process that only occurs in the presence of reducing equivalents like NADH.

<p>False (B)</p> Signup and view all the answers

The glycolytic breakdown of glucose can be the sole source of metabolic energy for certain mammalian tissues.

<p>True (A)</p> Signup and view all the answers

During gluconeogenesis, carbon skeletons can be formed from amino acids.

<p>True (A)</p> Signup and view all the answers

The Krebs cycle accepts carbon compounds derived from carbohydrates, lipids, or proteins.

<p>True (A)</p> Signup and view all the answers

Excess amino nitrogen is eliminated through ammonium production in the body.

<p>False (B)</p> Signup and view all the answers

Metabolites can only refer to products of metabolic reactions.

<p>False (B)</p> Signup and view all the answers

Stage II of catabolism involves the complete degradation of building blocks to simple intermediates.

<p>False (B)</p> Signup and view all the answers

Metabolites absorbed via the hepatic portal vein are initially directed to the lungs.

<p>False (B)</p> Signup and view all the answers

Many anaerobic microorganisms rely solely on the process of oxidative phosphorylation for energy.

<p>False (B)</p> Signup and view all the answers

Catabolic reactions capture energy in the form of ATP during the breakdown of biomolecules.

<p>True (A)</p> Signup and view all the answers

Biosynthetic reactions in anabolism depend solely on the availability of ATP.

<p>False (B)</p> Signup and view all the answers

Glycolysis consists of eight steps in total.

<p>False (B)</p> Signup and view all the answers

Fructose 1,6-bisphosphate is cleaved into two triose phosphates.

<p>True (A)</p> Signup and view all the answers

The first step of glycolysis involves the transfer of a phosphoryl group from ADP to glucose.

<p>False (B)</p> Signup and view all the answers

The enzyme phosphoglycerate kinase is responsible for the oxidation of glyceraldehyde 3-phosphate.

<p>False (B)</p> Signup and view all the answers

One molecule of glucose yields two molecules of glyceraldehyde 3-phosphate in glycolysis.

<p>True (A)</p> Signup and view all the answers

The pay-off phase of glycolysis conserves free energy in the form of NADH.

<p>False (B)</p> Signup and view all the answers

Phosphofructokinase-1 catalyzes the isomerization of glucose 6-phosphate to fructose 6-phosphate.

<p>False (B)</p> Signup and view all the answers

Dihydroxyacetone phosphate is an aldose sugar.

<p>False (B)</p> Signup and view all the answers

What is the primary purpose of catabolism in metabolic processes?

<p>To break down complex molecules and release energy (D)</p> Signup and view all the answers

During which stage of catabolism is pyruvate formed from building blocks?

<p>Stage II (B)</p> Signup and view all the answers

What role does acetyl-CoA play in the metabolic processes?

<p>It is a key intermediate for the final oxidation of energy-rich molecules (D)</p> Signup and view all the answers

Which of the following compounds can serve as starting materials for anabolic reactions?

<p>Acetyl-CoA (A)</p> Signup and view all the answers

What is a significant function of the Krebs cycle in cellular metabolism?

<p>It oxidizes acetyl-CoA completely to CO2 (D)</p> Signup and view all the answers

Which of the following statements about anabolic reactions is incorrect?

<p>They generate ATP as a main product. (B)</p> Signup and view all the answers

Which process is involved in the transformation of simple intermediates into building blocks?

<p>Catabolism (C)</p> Signup and view all the answers

What is formed from the carbon skeletons that remain after transamination?

<p>Glucose (B)</p> Signup and view all the answers

Which amino acids are primarily supplied in the diet?

<p>Essential amino acids (A)</p> Signup and view all the answers

What occurs during the final oxidation of acetyl-CoA in metabolic pathways?

<p>NADH and FADH2 are produced, which are used for energy (A)</p> Signup and view all the answers

What metabolic function does the liver perform with excess glucose?

<p>Stores it as glycogen (B)</p> Signup and view all the answers

Which compound is a product of glycolysis?

<p>Both B and C (B)</p> Signup and view all the answers

What happens to amino nitrogen after deamination in the body?

<p>It is removed as urea (D)</p> Signup and view all the answers

Where does glycolysis occur within a cell?

<p>Cytoplasm (D)</p> Signup and view all the answers

What is the primary function of acetyl-CoA derived from β-oxidation?

<p>To provide energy in the citric acid cycle (B)</p> Signup and view all the answers

Which type of tissues rely solely on glycolysis for metabolic energy?

<p>Brain and sperm cells (B)</p> Signup and view all the answers

What is a key role of the pentose phosphate pathway?

<p>To provide reducing equivalents and ribose (B)</p> Signup and view all the answers

During starvation, what becomes an important source of energy?

<p>Ketone bodies formed from acetyl-CoA (C)</p> Signup and view all the answers

Which of the following molecules can be derived from the intermediates of glycolysis?

<p>Nucleotides (B)</p> Signup and view all the answers

What is the significance of the conversion of pyruvate to acetyl-CoA?

<p>It allows entry into the citric acid cycle (A)</p> Signup and view all the answers

What is a primary source for fatty acid synthesis?

<p>Acetyl-CoA derived from carbohydrate (C)</p> Signup and view all the answers

In which organ is acetoacetate formed, and what is its primary function?

<p>Liver; as an energy reserve (B)</p> Signup and view all the answers

Which molecule is primarily generated during the rapid phase of glycolysis?

<p>Lactate (B)</p> Signup and view all the answers

What is the main purpose of glycogen in the body?

<p>To serve as a quick energy source (B)</p> Signup and view all the answers

What is the first product formed from the phosphorylation of glucose in glycolysis?

<p>Glucose 6-phosphate (C)</p> Signup and view all the answers

Which enzyme catalyzes the conversion of glucose 6-phosphate to fructose 6-phosphate?

<p>Phosphohexose isomerase (C)</p> Signup and view all the answers

In the pay-off phase of glycolysis, what are the two molecules primarily formed from one glucose molecule?

<p>Two molecules of glyceraldehyde 3-phosphate (A)</p> Signup and view all the answers

What type of reaction occurs during Step 4 of glycolysis when fructose 1,6-bisphosphate is cleaved?

<p>Aldol condensation (A)</p> Signup and view all the answers

During the oxidation of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, what is released?

<p>NADH (C)</p> Signup and view all the answers

Which enzyme is responsible for transferring a phosphoryl group from 1,3-bisphosphoglycerate to ADP?

<p>Phosphoglycerate kinase (A)</p> Signup and view all the answers

What is the purpose of the preparatory phase in glycolysis?

<p>Convert glucose to fructose-1,6-bisphosphate (C)</p> Signup and view all the answers

Which of the following accurately describes the inter-conversion of triose phosphates in glycolysis?

<p>Only one triose phosphate is utilized in subsequent glycolysis steps (B)</p> Signup and view all the answers

Flashcards

What is metabolism?

The sum of all chemical reactions that occur within a living organism.

What is a metabolic pathway?

A series of enzyme-catalyzed steps that produce a specific product.

What is catabolism?

The process of breaking down complex molecules into simpler ones, releasing energy.

What is anabolism?

The process of building complex molecules from simpler ones, requiring energy.

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What is a metabolite?

The starting materials or intermediates in metabolic reactions.

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What is ATP?

The energy currency of cells, used to power anabolic reactions.

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What happens in Stage I of Catabolism?

The first stage of catabolism where large molecules are broken down into their building blocks.

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What happens in Stage II of Catabolism?

The second stage of catabolism where building blocks are further broken down into simple intermediates like pyruvate and acetyl-CoA.

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Glycolysis

The metabolic pathway that breaks down glucose into pyruvate and lactate. It can occur with or without oxygen.

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Aerobic Metabolism

The process of converting pyruvate into acetyl-CoA, which then enters the citric acid cycle for complete oxidation to CO2 and H2O.

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Glycogen

A storage form of glucose, primarily found in skeletal muscle and liver.

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Pentose Phosphate Pathway

A metabolic pathway that generates reducing equivalents (such as NADPH) for biosynthesis. It also produces ribose, crucial for nucleotide and nucleic acid formation.

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β-oxidation

The breakdown of fatty acids into acetyl-CoA. This process releases energy.

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Triacylglycerol (Fat)

Storage form of fatty acids in the body. They serve as the primary source of energy reserves.

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Ketone Bodies

Ketone bodies are alternative water-soluble fuels used by tissues during conditions like starvation. Acetoacetate is the parent ketone body.

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Essential Amino Acids

Amino acids are essential for protein synthesis. Some must be obtained from the diet.

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Non-essential amino acids

Amino acids that can be synthesized by the body from other amino acids or intermediates. They don't need to be directly consumed.

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Transamination

The process where the amino group (NH2) from an amino acid is transferred to a keto acid, forming a new amino acid.

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Deamination

The process where the amino group (NH2) is removed from an amino acid, forming ammonia (NH3) and a keto acid. This is a key step in amino acid catabolism.

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Liver's role in metabolism

The liver is the main organ responsible for regulating the levels of glucose and amino acids in the blood. It takes up excess nutrients and stores them.

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Glycogenesis

The process where glucose is converted into glycogen, a storage form of glucose, primarily in the liver.

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Glycogenolysis

The process where glycogen is broken down into glucose, providing energy for the body.

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Lipogenesis

The process where glucose is converted into fatty acids and stored as triglycerides.

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What is the first step in glycolysis?

Glucose is activated by adding a phosphate group to its C-6 position, forming glucose 6-phosphate.

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How is glucose 6-phosphate converted to fructose 6-phosphate?

Glucose 6-phosphate, an aldose, is converted to fructose 6-phosphate, a ketose. This reaction is catalyzed by phosphohexose isomerase.

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What happens in the second phosphorylation step of glycolysis?

Another phosphate group is added to fructose 6-phosphate at C-1, forming fructose 1,6-bisphosphate. This reaction is catalyzed by phosphofructokinase-1 (PFK-1).

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What happens to fructose 1,6-bisphosphate?

Fructose 1,6-bisphosphate is cleaved into two three-carbon molecules: glyceraldehyde 3- phosphate and dihydroxyacetone phosphate. This reaction is catalyzed by aldolase.

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What is the purpose of the inter-conversion of the triose phosphates?

Dihydroxyacetone phosphate, a ketose, is converted to glyceraldehyde 3-phosphate, an aldose. This reaction is catalyzed by triose phosphate isomerase.

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What happens during the oxidation of glyceraldehyde 3-phosphate?

Glyceraldehyde 3-phosphate is oxidized to 1,3-bisphosphoglycerate, and NAD+ is reduced to NADH. This reaction is catalyzed by glyceraldehyde 3-phosphate dehydrogenase.

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How is ATP generated in the pay-off phase of glycolysis?

The high-energy phosphate group from 1,3-bisphosphoglycerate is transferred to ADP, forming ATP and 3-phosphoglycerate. This reaction is catalyzed by phosphoglycerate kinase.

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What is the pay-off phase in glycolysis?

The pay-off phase is the second phase, after the preparatory phase. It includes the energy-generating steps where ATP is produced.

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Study Notes

Biochemistry Module: Introduction to Metabolism & Carbohydrate Metabolism

  • The module covers biochemistry, metabolism, and carbohydrate metabolism.
  • The lecturer is Dr Mohammed Mansour, a Senior Lecturer in Biomedical Science at London South Bank University.
  • The module's duration is from 12:00 to 15:00.
  • Online access via VLE is used.
  • A one-time code for access is SO-HB-BK.

Catabolic and Anabolic Reactions

  • Catabolism is a degradative process that breaks down complex molecules into simpler ones, releasing energy.
  • Anabolism is a biosynthetic process building complex molecules from simpler ones, requiring energy.
  • Complex organic molecules like carbohydrates, lipids, and proteins are broken down into simpler molecules like simple sugars, fatty acids, and amino acids.
  • The released energy is in the form of ATP (adenosine triphosphate) and NADPH.

Introduction to Metabolism

  • Metabolism encompasses all chemical reactions within a living system.
  • A metabolic pathway is a series of enzymatic reactions that produce desired products.
  • Metabolites are substrates, intermediates, or products in metabolic reactions.
  • Metabolism is classified into catabolism and anabolism.
  • Enzymes act as biological catalysts in these reactions.

Outline of Metabolism

  • Stage I of catabolism: conversion of complex molecules into their building blocks. (e.g., polysaccharides to monosaccharides, lipids to fatty acids and glycerol, proteins to amino acids).
  • Stage II of catabolism: formation of simple intermediates (e.g., pyruvate and acetyl-CoA).
  • Stage III of catabolism: final oxidation of acetyl-CoA (e.g., Krebs cycle).

Carbohydrate Metabolism

  • Carbohydrate metabolism focuses on glucose's fate, including glycolysis.
  • Glycolysis converts glucose to pyruvate and lactate in all cells.
  • Anaerobic conditions result in only lactate as a product.
  • Aerobic tissues utilize oxygen, converting pyruvate to acetyl-CoA, enabling entry into the citric acid cycle for complete oxidation into CO2 and H2O, releasing ATP.
  • Glucose is a major fuel source for many tissues and is involved in various processes.

Other Metabolic Pathways

  • Glycogen synthesis: Glucose is converted into glycogen, primarily in liver and muscle tissue.
  • Pentose phosphate pathway: A source of reducing equivalents (2H) for biosynthesis (e.g., fatty acids), and a source of ribose for nucleotides and nucleic acid formation.
  • Fatty acid synthesis/biosynthesis: Acetyl-CoA is the source for fatty acid synthesis. Fatty acids are either derived from dietary components or through de novo synthesis by acetyl-CoA.
  • Fatty acid oxidation: Fatty acids' breakdown into acetyl-CoA (β-oxidation) and incorporation into triacylglycerol.
  • Amino acid metabolism: Necessary for protein synthesis. Some amino acids are essential (dietary) and others are non-essential (formed from intermediates). Transamination and deamination are key processes.
  • Ketone bodies: The liver produces ketone bodies, a source of alternative water-soluble metabolic fuel during conditions like starvation.

Metabolic Pathways - Levels of Organization

  • Metabolic pathways are studied at different levels:
    • Subcellular level: organelles like mitochondria and cytosol have specific roles and a pattern.
    • Tissue and organ level: the nature of substrates entering and metabolites leaving tissues and organs are observed.
  • Blood circulation integrates metabolism, carrying amino acids and glucose from digestion to other body parts.

Liver's Role in Metabolism

  • The liver plays a primary role in regulating metabolite levels, particularly for glucose.
  • Glucose homeostasis is regulated through glycogen storage (glycogenesis) or conversion to fat (lipogenesis).
  • Between meals, the liver uses stored glycogen for glucose release.

Glycolysis

  • Glycolysis is a universal central pathway of glucose breakdown in most cells.
  • In some cells (erythrocytes, renal medulla, brain, and sperm), it is the sole metabolic energy source.
  • It occurs in the cytoplasm.
  • The process breaks down glucose into pyruvate, with energy conservation in ATP form.
  • Steps involved include phosphorylation phases and energy-conservation phases.
  • In anaerobic conditions, pyruvate is reduced to lactate, regenerating NAD+.

Additional Notes

  • The provided slides provide an outline of the biochemistry modules' content, concentrating on metabolism and carbohydrate metabolism.
  • A variety of reactions, processes, and molecules are discussed.
  • Key concepts like catabolism, anabolism, glycolysis, and enzymes are highlighted.
  • The importance of different organs like the liver in managing metabolic processes is emphasized.

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