29 Questions
What is the role of an enzyme in a biological reaction?
To speed up the reaction without being changed
What is the result of malfunctions in enzyme reactions?
Diseases occur
What is the mechanism of HIV1 protease inhibitors?
They bind to the active site and block the enzyme's activity
What is the importance of enzymes in biological processes?
They are involved in all biological processes
What is the term for the area of the enzyme where substrate(s) bind?
Active site
What is the purpose of catalysis in biological reactions?
To control the reaction
What is the primary function of biological catalysts, such as enzymes?
To speed up chemical reactions by reducing the activation energy
What is the term for the minimum energy required for a reaction to occur?
Activation energy
What is the purpose of measuring enzyme activity?
To understand the rate of a reaction
What is the term for the state in which the reactants and products are equally balanced?
Equilibrium
What is the characteristic of an enzyme's active site that allows it to bind to a specific substrate?
Induced fit
What is the term for the measure of disorder or randomness in a system?
Entropy
What is the primary reason for starting with a high concentration of substrate and a much lower concentration of enzyme?
To ensure the enzyme is fully saturated
What is being measured in an enzyme kinetics experiment?
The decrease in substrate or the increase in product
What is the purpose of measuring the reaction rate at different starting substrate concentrations?
To determine the Km value
What is the complex formed during the enzyme-catalyzed reaction?
ES
What is the equation that represents the enzyme-catalyzed reaction?
E + S → ES → EP → E + P
What can be measured during an enzyme-catalyzed reaction?
The decrease in substrate and the increase in product
What is the difference between the substrate and product in the catalase reaction?
The substrate is a liquid, while the product is a gas
Why can NITROSEFIN be used to study the degradation of penicillin by β-lactamase?
Because NITROSEFIN is a colored molecule that changes intensity during the reaction
What is the role of the enzyme catalyst in the reaction?
To speed up the reaction without being consumed
What is a common feature of many substrates and products in biology?
They absorb specific wavelengths of light
What is the purpose of measuring the amount of time the enzyme is bound to the substrate?
To determine the rate of the reaction
Why do we need to use a spectrophotometer to measure the rate of an enzyme-catalysed reaction?
Because many enzymes absorb in the ultraviolet range
What is the purpose of using a stopped flow method in enzyme kinetics?
To measure the rate of very fast reactions
What is the significance of the maximum rate of an enzyme-catalysed reaction?
It is the fastest rate that the reaction can go in the presence of the enzyme
What is the importance of understanding the affinity of an enzyme for its substrate?
It determines how well the substrate binds to the active site
What is the purpose of electrophoresis in enzyme kinetics?
To separate big fragments from small fragments
What is the significance of using a high concentration of substrate and a low concentration of enzyme in enzyme kinetics experiments?
To ensure that the enzyme is fully saturated with substrate
Learn about enzymes, catalysts, and substrates in biochemical reactions, including their definitions, active sites, and how they affect the organism's state.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
