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Questions and Answers
What is the common name of the separation method that has been used for thousands of years?
What is the common name of the separation method that has been used for thousands of years?
- Gravitational sedimentation (correct)
- Floatation
- Electrostatic separation
- Centrifugation
What is an example of the use of gravitational sedimentation?
What is an example of the use of gravitational sedimentation?
- Separating salt from sugar
- Separating gold particles from sand (correct)
- Separating water from oil
- Separating iron from copper
What is the time period for which gravitational sedimentation has been used?
What is the time period for which gravitational sedimentation has been used?
- Decades of years
- Millions of years
- Hundreds of years
- Thousands of years (correct)
What type of particles are separated from sand using gravitational sedimentation?
What type of particles are separated from sand using gravitational sedimentation?
What is the basis of the gravitational sedimentation separation method?
What is the basis of the gravitational sedimentation separation method?
What is the technique used to study molecular interactions and the formation of enzyme complexes?
What is the technique used to study molecular interactions and the formation of enzyme complexes?
What is the result of the formation of enzyme complexes?
What is the result of the formation of enzyme complexes?
What is the significance of studying molecular interactions?
What is the significance of studying molecular interactions?
What is the role of enzyme complexes in molecular interactions?
What is the role of enzyme complexes in molecular interactions?
What is the benefit of using Chromatin Immunoprecipitation in studying molecular interactions?
What is the benefit of using Chromatin Immunoprecipitation in studying molecular interactions?
What is the primary purpose of rate-zonal centrifugation?
What is the primary purpose of rate-zonal centrifugation?
In rate-zonal centrifugation, how is the sample loaded?
In rate-zonal centrifugation, how is the sample loaded?
What is the name of the technique that separates particles based on their size and density?
What is the name of the technique that separates particles based on their size and density?
What is the function of the density gradient medium in rate-zonal centrifugation?
What is the function of the density gradient medium in rate-zonal centrifugation?
What is an alternative name for rate-zonal centrifugation?
What is an alternative name for rate-zonal centrifugation?
What is the main function of SAT in the metabolic pathway?
What is the main function of SAT in the metabolic pathway?
What is the name of the process in which L-serine is converted to cysteine?
What is the name of the process in which L-serine is converted to cysteine?
What is the purpose of using analytical ultracentrifugation in the study of SAT?
What is the purpose of using analytical ultracentrifugation in the study of SAT?
What is the starting material for the production of cysteine in bacteria?
What is the starting material for the production of cysteine in bacteria?
What is the role of SAT in the bacterial cell?
What is the role of SAT in the bacterial cell?
What is the purpose of centrifugation at 100,000 g for 48 hours?
What is the purpose of centrifugation at 100,000 g for 48 hours?
What is the result of centrifugation at 100,000 g for 48 hours?
What is the result of centrifugation at 100,000 g for 48 hours?
What is the density of the layer that floats after centrifugation at 100,000 g for 48 hours?
What is the density of the layer that floats after centrifugation at 100,000 g for 48 hours?
What is the name of the layer that floats after centrifugation at 100,000 g for 48 hours?
What is the name of the layer that floats after centrifugation at 100,000 g for 48 hours?
What is the unit of measurement for the force applied during centrifugation?
What is the unit of measurement for the force applied during centrifugation?
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Study Notes
Gravitational Sedimentation
- Gravitational sedimentation is a well-known separation method used for thousands of years, for example, to separate gold particles from sand.
Rate-Zonal Centrifugation
- In rate-zonal centrifugation, the sample is loaded as a thin layer on the top of a density gradient medium.
- This technique was used to study the semi-conservative replication of DNA.
Enzyme Study
- Analytical ultracentrifugation methods were used to study the serine acetyltransferase (SAT) enzyme in E. coli.
- The goal was to determine the number of protein subunits present in the functional enzyme.
Bacterial Metabolic Pathway
- In bacteria, L-serine is converted to cysteine in a two-step process.
- SAT catalyzes the first reaction in this metabolic pathway, known as "sulfur fixation".
Centrifugation Process
- Centrifugation at 100,000 g for 48 hours produces a floating layer of HDL.
- The same process, with the same conditions, produces a floating layer of LDL.
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