Biochemistry Laboratory Techniques
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Questions and Answers

What is the common name of the separation method that has been used for thousands of years?

  • Gravitational sedimentation (correct)
  • Floatation
  • Electrostatic separation
  • Centrifugation

    • What is an example of the use of gravitational sedimentation?

  • Separating salt from sugar
  • Separating gold particles from sand (correct)
  • Separating water from oil
  • Separating iron from copper

    • What is the time period for which gravitational sedimentation has been used?

  • Decades of years
  • Millions of years
  • Hundreds of years
  • Thousands of years (correct)

    • What type of particles are separated from sand using gravitational sedimentation?

    <p>Gold particles</p> Signup and view all the answers

    What is the basis of the gravitational sedimentation separation method?

    <p>Density difference</p> Signup and view all the answers

    What is the technique used to study molecular interactions and the formation of enzyme complexes?

    <p>Chromatin Immunoprecipitation</p> Signup and view all the answers

    What is the result of the formation of enzyme complexes?

    <p>Structural changes in the enzyme</p> Signup and view all the answers

    What is the significance of studying molecular interactions?

    <p>To understand the mechanism of gene expression</p> Signup and view all the answers

    What is the role of enzyme complexes in molecular interactions?

    <p>To regulate enzyme activity</p> Signup and view all the answers

    What is the benefit of using Chromatin Immunoprecipitation in studying molecular interactions?

    <p>It allows for the study of DNA-protein interactions</p> Signup and view all the answers

    What is the primary purpose of rate-zonal centrifugation?

    <p>To separate particles based on their size and density</p> Signup and view all the answers

    In rate-zonal centrifugation, how is the sample loaded?

    <p>As a thin layer on top of a density gradient medium</p> Signup and view all the answers

    What is the name of the technique that separates particles based on their size and density?

    <p>Rate-zonal centrifugation</p> Signup and view all the answers

    What is the function of the density gradient medium in rate-zonal centrifugation?

    <p>To provide a density gradient for particle separation</p> Signup and view all the answers

    What is an alternative name for rate-zonal centrifugation?

    <p>Velocity sedimentation</p> Signup and view all the answers

    What is the main function of SAT in the metabolic pathway?

    <p>Catalyze the first reaction in sulfur fixation</p> Signup and view all the answers

    What is the name of the process in which L-serine is converted to cysteine?

    <p>Sulfur fixation</p> Signup and view all the answers

    What is the purpose of using analytical ultracentrifugation in the study of SAT?

    <p>To elucidate the number of protein subunits present in the functional enzyme</p> Signup and view all the answers

    What is the starting material for the production of cysteine in bacteria?

    <p>L-serine</p> Signup and view all the answers

    What is the role of SAT in the bacterial cell?

    <p>To catalyze the first reaction in sulfur fixation</p> Signup and view all the answers

    What is the purpose of centrifugation at 100,000 g for 48 hours?

    <p>To separate HDL from LDL</p> Signup and view all the answers

    What is the result of centrifugation at 100,000 g for 48 hours?

    <p>A layer of HDL at the top of the tube</p> Signup and view all the answers

    What is the density of the layer that floats after centrifugation at 100,000 g for 48 hours?

    <p>Lower than the surrounding liquid</p> Signup and view all the answers

    What is the name of the layer that floats after centrifugation at 100,000 g for 48 hours?

    <p>HDL</p> Signup and view all the answers

    What is the unit of measurement for the force applied during centrifugation?

    <p>g</p> Signup and view all the answers

    Study Notes

    Gravitational Sedimentation

    • Gravitational sedimentation is a well-known separation method used for thousands of years, for example, to separate gold particles from sand.

    Rate-Zonal Centrifugation

    • In rate-zonal centrifugation, the sample is loaded as a thin layer on the top of a density gradient medium.
    • This technique was used to study the semi-conservative replication of DNA.

    Enzyme Study

    • Analytical ultracentrifugation methods were used to study the serine acetyltransferase (SAT) enzyme in E. coli.
    • The goal was to determine the number of protein subunits present in the functional enzyme.

    Bacterial Metabolic Pathway

    • In bacteria, L-serine is converted to cysteine in a two-step process.
    • SAT catalyzes the first reaction in this metabolic pathway, known as "sulfur fixation".

    Centrifugation Process

    • Centrifugation at 100,000 g for 48 hours produces a floating layer of HDL.
    • The same process, with the same conditions, produces a floating layer of LDL.

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    Description

    This quiz covers various laboratory techniques used in biochemistry, including gravitational sedimentation and rate-zonal centrifugation.

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