Biochemistry Lab 3: Denaturing of Proteins
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Questions and Answers

What is denaturation of proteins?

  • A process where a protein increases in size due to the addition of amino acids
  • A process where a protein loses its native shape and becomes biologically inactive (correct)
  • A process where a protein gains its native shape
  • A process where a protein changes its function

Which factor causes denaturation of proteins by disrupting hydrophobic interactions?

  • Acid
  • Radiation
  • Alcohol (correct)
  • Heat

How does changing pH affect protein denaturation?

  • It enhances the protein's biological activity
  • It denatures the protein by altering charges on side chains (correct)
  • It decreases the size of the protein
  • It has no effect on the protein's structure

Which of the following is NOT an agent causing denaturation of proteins?

<p>Enzymes (C)</p> Signup and view all the answers

What happens to a denatured antibody?

<p>It loses its ability to bind to antigens (C)</p> Signup and view all the answers

Why does increasing temperature lead to protein denaturation?

<p>It disrupts the attractive forces that hold the protein's shape (B)</p> Signup and view all the answers

What is the denaturation process that occurs at higher temperatures called?

<p>Heat denaturation (A)</p> Signup and view all the answers

During denaturation, which type of bond is NOT broken?

<p>Peptide bonds (D)</p> Signup and view all the answers

What is the purpose of adding egg white to boiling water in the experiment described?

<p>To observe heat denaturation (D)</p> Signup and view all the answers

What happens when egg white is subjected to vigorous shaking in a container?

<p>Mechanical denaturation (A)</p> Signup and view all the answers

Which component is NOT used in the experiment described for observing denaturation?

<p>Vinegar (B)</p> Signup and view all the answers

What bonds are broken during denaturation according to the text?

<p>Disulfide bonds and hydrophobic interactions (C)</p> Signup and view all the answers

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