Podcast
Questions and Answers
What is the primary purpose of biochemical profiling?
What is the primary purpose of biochemical profiling?
- To eliminate the need for any laboratory tests
- To identify all diseases present in an individual
- To assess the physiological status and improve treatment plans (correct)
- To measure the exact concentration of glucose only
Which factor should NOT be considered when selecting biochemical tests?
Which factor should NOT be considered when selecting biochemical tests?
- The best interest of the patient
- The statistical significance of all possible tests (correct)
- The potential cost of unnecessary tests
- The efficiency of the tests
In colorimetric assays, what is used to detect the presence of glucose?
In colorimetric assays, what is used to detect the presence of glucose?
- Horse Radish Peroxidase (correct)
- D-Gluconic Acid
- Glucose Oxidase
- Hydrogen peroxide
What does optical density (OD) measure in biochemical testing?
What does optical density (OD) measure in biochemical testing?
How can unknown concentrations of biomolecules be determined using a known sample plot?
How can unknown concentrations of biomolecules be determined using a known sample plot?
What is a primary characteristic of a screening test?
What is a primary characteristic of a screening test?
Which statement accurately differentiates plasma from serum?
Which statement accurately differentiates plasma from serum?
What is an example of a specific function test?
What is an example of a specific function test?
What process is used to separate light and heavy fluids in blood samples?
What process is used to separate light and heavy fluids in blood samples?
Why is the serum urea level important in medical testing?
Why is the serum urea level important in medical testing?
What type of sample can be used for both specific function tests and screening tests?
What type of sample can be used for both specific function tests and screening tests?
How do electrolytes influence health assessment?
How do electrolytes influence health assessment?
What distinguishes a specific function test from a screening test?
What distinguishes a specific function test from a screening test?
What is the primary use of venous plasma sampling?
What is the primary use of venous plasma sampling?
Which of the following statements regarding arterial plasma sampling is true?
Which of the following statements regarding arterial plasma sampling is true?
For which patient scenario is capillary sampling most appropriately used?
For which patient scenario is capillary sampling most appropriately used?
What is produced from the breakdown of protein and amino acids in the liver?
What is produced from the breakdown of protein and amino acids in the liver?
Which enzyme is used to convert urea into ammonium?
Which enzyme is used to convert urea into ammonium?
During the measurement of blood urea, what is the initial blood sample processing step?
During the measurement of blood urea, what is the initial blood sample processing step?
What happens to NADH during the conversion of ammonium and 2-oxoglutarate into glutamate?
What happens to NADH during the conversion of ammonium and 2-oxoglutarate into glutamate?
What condition is indicated by hypoxaemia?
What condition is indicated by hypoxaemia?
What does a high level of urea typically indicate?
What does a high level of urea typically indicate?
Which condition is NOT associated with low urea levels?
Which condition is NOT associated with low urea levels?
Which component of the Troponin complex indicates cardiac muscle damage?
Which component of the Troponin complex indicates cardiac muscle damage?
What is the role of the reference electrode in ion selective electrodes (ISE)?
What is the role of the reference electrode in ion selective electrodes (ISE)?
What factors can affect the reference ranges for medical tests?
What factors can affect the reference ranges for medical tests?
In the detection of Troponin, what does the second antibody attach to?
In the detection of Troponin, what does the second antibody attach to?
What does a potential difference between the reference electrode and sample electrode indicate?
What does a potential difference between the reference electrode and sample electrode indicate?
What does abnormal levels of Troponin indicate?
What does abnormal levels of Troponin indicate?
Flashcards
Biochemical profiling
Biochemical profiling
Assessing the physiological status of an individual to support diagnosis and tailor treatment plans.
Optical Density (OD)
Optical Density (OD)
A unit used to measure the amount of light absorbed by a solution, indicating the number of molecules.
Colorimetric assays
Colorimetric assays
Methods that measure biomolecules by generating a color change, based on light absorption.
Glucose measurement (example)
Glucose measurement (example)
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Determining unknown concentrations
Determining unknown concentrations
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Screening test
Screening test
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Specific function test
Specific function test
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Sample types
Sample types
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Urea and electrolytes
Urea and electrolytes
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Serum urea level
Serum urea level
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Electrolytes
Electrolytes
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Plasma
Plasma
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Serum
Serum
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Venous Blood Sampling
Venous Blood Sampling
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Arterial Blood Sampling
Arterial Blood Sampling
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Capillary Blood Sampling
Capillary Blood Sampling
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Urea
Urea
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Urea Production
Urea Production
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Urea Excretion
Urea Excretion
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Urease Enzyme
Urease Enzyme
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Glutamate Dehydrogenase
Glutamate Dehydrogenase
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Urea Measurement
Urea Measurement
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Electrolyte Imbalance
Electrolyte Imbalance
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Reference Ranges
Reference Ranges
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Troponin
Troponin
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TnC, TnI, TnT
TnC, TnI, TnT
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Myocardial Infarction
Myocardial Infarction
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cTnI Detection Steps
cTnI Detection Steps
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Ion Selective Electrodes (ISE)
Ion Selective Electrodes (ISE)
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Study Notes
Biochemical Tests
- Biochemical profiling assesses an individual's physiological status, aiding diagnosis and improving treatment plans.
- Efficient test selection, valid justification, and patient benefit are crucial.
- Biomolecules are measured by how they absorb light (optical density).
- Colorimetric assays use color changes to quantify biomolecules.
- Glucose is converted to D-Gluconic acid by glucose oxidase, producing hydrogen peroxide.
- Hydrogen peroxide reacts with HRP to produce a measurable color change (540-570nm wavelength).
- Unknown concentrations are determined by plotting a standard curve with known samples.
Screening vs. Specific Function Tests
- Screening tests provide general information rapidly.
- Specific function tests offer detailed information on specific systems.
- Common samples for testing include blood, urine, saliva, and sweat.
- Urea and electrolytes are frequently analyzed, reflecting renal function and electrolyte balance.
- Plasma (unclotted) or serum (clotted) can be used. Plasma is often preferred for specific tests.
- Venous sampling is common for analyzing blood cell contents.
- Arterial sampling measures gases (oxygen, carbon dioxide, pH).
- Capillary sampling is suitable for infants, fragile veins, and burns.
Urea and Electrolytes
- Urea is a protein/amino acid breakdown product, processed by the liver, and excreted by the kidneys.
- Urea is measured by converting it to ammonium, which then yields a measurable color change.
- High urea often indicates kidney problems or dehydration.
- Low urea may indicate liver disease, starvation, or pregnancy.
- Ion-selective electrodes (ISEs) measure electrolytes. ISES have a reference and a sample electrode.
- Electrolyte imbalances can result from renal issues, dehydration, or liver failure.
Reference Ranges
- Reference ranges indicate healthy values, influenced by factors like sex, ethnicity, and socioeconomic status.
- Reference ranges are important for evaluating test results.
Myocardial Infarction (MI) and Troponin
- Troponin is a protein in heart muscle cells.
- Elevated troponin levels in blood indicate possible heart muscle damage (ischemic cardiac damage).
- Troponin levels are analyzed using special antibodies and a luminometer.
Patient Case Study
- Diabetic ketoacidosis results in the increased metabolic breakdown of fats, producing ketone bodies.
- High levels of ketone bodies can cause acidosis (lower blood pH).
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