CVR MCB

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Questions and Answers

What is the primary purpose of biochemical profiling?

  • To eliminate the need for any laboratory tests
  • To identify all diseases present in an individual
  • To assess the physiological status and improve treatment plans (correct)
  • To measure the exact concentration of glucose only

Which factor should NOT be considered when selecting biochemical tests?

  • The best interest of the patient
  • The statistical significance of all possible tests (correct)
  • The potential cost of unnecessary tests
  • The efficiency of the tests

In colorimetric assays, what is used to detect the presence of glucose?

  • Horse Radish Peroxidase (correct)
  • D-Gluconic Acid
  • Glucose Oxidase
  • Hydrogen peroxide

What does optical density (OD) measure in biochemical testing?

<p>The amount of light absorbed by a solution (A)</p> Signup and view all the answers

How can unknown concentrations of biomolecules be determined using a known sample plot?

<p>By tracing the OD value horizontally to the line of best fit (D)</p> Signup and view all the answers

What is a primary characteristic of a screening test?

<p>It reflects the general conditions of an individual. (C)</p> Signup and view all the answers

Which statement accurately differentiates plasma from serum?

<p>Plasma contains anticoagulants whereas serum does not. (A)</p> Signup and view all the answers

What is an example of a specific function test?

<p>Urea and electrolytes analysis (B)</p> Signup and view all the answers

What process is used to separate light and heavy fluids in blood samples?

<p>Centrifugation (B)</p> Signup and view all the answers

Why is the serum urea level important in medical testing?

<p>It indicates the effectiveness of renal function. (B)</p> Signup and view all the answers

What type of sample can be used for both specific function tests and screening tests?

<p>Blood and urine (D)</p> Signup and view all the answers

How do electrolytes influence health assessment?

<p>They reflect general conditions and renal function. (B)</p> Signup and view all the answers

What distinguishes a specific function test from a screening test?

<p>Specific function tests offer detailed information. (C)</p> Signup and view all the answers

What is the primary use of venous plasma sampling?

<p>Analyzing blood cell contents (C)</p> Signup and view all the answers

Which of the following statements regarding arterial plasma sampling is true?

<p>It measures partial pressure of gases and pH. (A)</p> Signup and view all the answers

For which patient scenario is capillary sampling most appropriately used?

<p>Infants and patients with fragile veins (B)</p> Signup and view all the answers

What is produced from the breakdown of protein and amino acids in the liver?

<p>Urea (C)</p> Signup and view all the answers

Which enzyme is used to convert urea into ammonium?

<p>Urease (B)</p> Signup and view all the answers

During the measurement of blood urea, what is the initial blood sample processing step?

<p>Centrifuge to extract serum from clotted blood (C)</p> Signup and view all the answers

What happens to NADH during the conversion of ammonium and 2-oxoglutarate into glutamate?

<p>It is oxidized to NAD. (B)</p> Signup and view all the answers

What condition is indicated by hypoxaemia?

<p>Low oxygen tension in circulation (B)</p> Signup and view all the answers

What does a high level of urea typically indicate?

<p>Kidney function alteration or dehydration (A)</p> Signup and view all the answers

Which condition is NOT associated with low urea levels?

<p>Dehydration (D)</p> Signup and view all the answers

Which component of the Troponin complex indicates cardiac muscle damage?

<p>TnI (B), TnT (C)</p> Signup and view all the answers

What is the role of the reference electrode in ion selective electrodes (ISE)?

<p>To allow all ion influx into the electrode (D)</p> Signup and view all the answers

What factors can affect the reference ranges for medical tests?

<p>Biological sex, ethnicity, and socioeconomic status (D)</p> Signup and view all the answers

In the detection of Troponin, what does the second antibody attach to?

<p>Alkaline phosphatase (ALP) (A)</p> Signup and view all the answers

What does a potential difference between the reference electrode and sample electrode indicate?

<p>Specific ion concentration in the sample (A)</p> Signup and view all the answers

What does abnormal levels of Troponin indicate?

<p>Ischaemic cardiac damage (D)</p> Signup and view all the answers

Flashcards

Biochemical profiling

Assessing the physiological status of an individual to support diagnosis and tailor treatment plans.

Optical Density (OD)

A unit used to measure the amount of light absorbed by a solution, indicating the number of molecules.

Colorimetric assays

Methods that measure biomolecules by generating a color change, based on light absorption.

Glucose measurement (example)

Glucose is converted to a colored compound (measuring absorption at 540-570nm), this reaction lets scientists know how much glucose there is.

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Determining unknown concentrations

Using a graph created from known samples to find the concentration of unknown samples based on their optical density

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Screening test

A general health check that takes little time, reflecting the overall condition of a person.

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Specific function test

A detailed test performed after a screening test, focusing on specific organs or systems.

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Sample types

Various materials like blood (with serum/plasma) and urine, saliva, and sweat, used for analysis in medical tests.

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Urea and electrolytes

Examples of tests used that help assess kidney function and overall health by detecting imbalances in important chemical elements.

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Serum urea level

A blood test indicating the efficiency of the kidney function.

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Electrolytes

Chemicals in the blood that help maintain the body's balance and show how effective the kidneys are, and the general health.

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Plasma

The liquid part of blood that hasn't clotted.

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Serum

The liquid part of blood after it has clotted.

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Venous Blood Sampling

Blood drawn from a vein, commonly used for analyzing blood cell components like red and white blood cells.

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Arterial Blood Sampling

Blood drawn from an artery, used to measure oxygen, carbon dioxide, and pH levels.

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Capillary Blood Sampling

Blood drawn from a capillary, used for infants, patients with fragile veins, or burns.

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Urea

The breakdown product of protein and amino acid metabolism.

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Urea Production

Urea is produced in the liver when ammonia combines with CO2.

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Urea Excretion

Urea travels through the blood to the kidneys and is excreted in urine.

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Urease Enzyme

An enzyme used in urea measurement to convert urea into ammonium.

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Glutamate Dehydrogenase

An enzyme used in urea measurement to convert ammonium and 2-Oxoglutarate into glutamate.

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Urea Measurement

Urea levels indicate kidney function, with high levels suggesting potential problems or dehydration, while low levels could point to chronic liver disease, starvation, or pregnancy.

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Electrolyte Imbalance

An imbalance of electrolytes in the blood, often caused by chronic kidney malfunction, dehydration, or liver failure.

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Reference Ranges

The normal range of values for a specific test, indicating healthy levels. Factors like sex, ethnicity, and socioeconomic status can influence these ranges.

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Troponin

A protein found in heart muscle cells, used to detect heart damage. Abnormal levels indicate possible ischemic cardiac damage.

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TnC, TnI, TnT

Subunits of the Troponin complex. Detection of TnI and TnT specifically indicates cardiac muscle damage.

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Myocardial Infarction

A heart attack, caused by a blockage in the coronary arteries, leading to damage to the heart muscle.

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cTnI Detection Steps

Two antibodies are used to detect cTnI in a blood sample. One antibody is attached to magnetic beads and the other to an enzyme called alkaline phosphatase. Both bind to different parts of the cTnI molecule.

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Ion Selective Electrodes (ISE)

Two electrodes used to measure electrolytes. One electrode allows all ions to pass, while the other allows only specific ions, creating a potential difference that reflects the level of the target ion.

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Study Notes

Biochemical Tests

  • Biochemical profiling assesses an individual's physiological status, aiding diagnosis and improving treatment plans.
  • Efficient test selection, valid justification, and patient benefit are crucial.
  • Biomolecules are measured by how they absorb light (optical density).
  • Colorimetric assays use color changes to quantify biomolecules.
  • Glucose is converted to D-Gluconic acid by glucose oxidase, producing hydrogen peroxide.
  • Hydrogen peroxide reacts with HRP to produce a measurable color change (540-570nm wavelength).
  • Unknown concentrations are determined by plotting a standard curve with known samples.

Screening vs. Specific Function Tests

  • Screening tests provide general information rapidly.
  • Specific function tests offer detailed information on specific systems.
  • Common samples for testing include blood, urine, saliva, and sweat.
  • Urea and electrolytes are frequently analyzed, reflecting renal function and electrolyte balance.
  • Plasma (unclotted) or serum (clotted) can be used. Plasma is often preferred for specific tests.
  • Venous sampling is common for analyzing blood cell contents.
  • Arterial sampling measures gases (oxygen, carbon dioxide, pH).
  • Capillary sampling is suitable for infants, fragile veins, and burns.

Urea and Electrolytes

  • Urea is a protein/amino acid breakdown product, processed by the liver, and excreted by the kidneys.
  • Urea is measured by converting it to ammonium, which then yields a measurable color change.
  • High urea often indicates kidney problems or dehydration.
  • Low urea may indicate liver disease, starvation, or pregnancy.
  • Ion-selective electrodes (ISEs) measure electrolytes. ISES have a reference and a sample electrode.
  • Electrolyte imbalances can result from renal issues, dehydration, or liver failure.

Reference Ranges

  • Reference ranges indicate healthy values, influenced by factors like sex, ethnicity, and socioeconomic status.
  • Reference ranges are important for evaluating test results.

Myocardial Infarction (MI) and Troponin

  • Troponin is a protein in heart muscle cells.
  • Elevated troponin levels in blood indicate possible heart muscle damage (ischemic cardiac damage).
  • Troponin levels are analyzed using special antibodies and a luminometer.

Patient Case Study

  • Diabetic ketoacidosis results in the increased metabolic breakdown of fats, producing ketone bodies.
  • High levels of ketone bodies can cause acidosis (lower blood pH).

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