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Questions and Answers
What is the oxidation product of dissolved iron when exposed to oxygen in groundwater?
What is the oxidation product of dissolved iron when exposed to oxygen in groundwater?
- Fe2O3
- Fe(OH)2
- Fe(OH)3 (correct)
- Fe(HCO3)2
Which method is used to determine the concentration of manganese in water samples?
Which method is used to determine the concentration of manganese in water samples?
- Direct measurement without any reagents
- Spectrophotometry at 480 nm
- Using a Nessler cylinder without any dilution
- Heating and boiling the sample with ammonium persulfate (correct)
What is the maximum permissible value of iron in drinking water?
What is the maximum permissible value of iron in drinking water?
- 50 μg/L
- 200 μg/L (correct)
- 100 μg/L
- 250 mg/L
Which step is NOT part of the iron concentration measurement method using a Nessler cylinder?
Which step is NOT part of the iron concentration measurement method using a Nessler cylinder?
What is the wavelength used to check the concentration of sulphates in a water sample?
What is the wavelength used to check the concentration of sulphates in a water sample?
What does the biochemical oxygen demand (BOD) measure?
What does the biochemical oxygen demand (BOD) measure?
During which stage does nitrification occur in the BOD method?
During which stage does nitrification occur in the BOD method?
What is the purpose of the Winkler method in the BOD process?
What is the purpose of the Winkler method in the BOD process?
How many Winkler bottles are filled during the preparation of dilutions?
How many Winkler bottles are filled during the preparation of dilutions?
What organic compounds are oxidized in Stage I of the BOD method?
What organic compounds are oxidized in Stage I of the BOD method?
To achieve a 1:5 dilution, how much dilution water is needed for a total sample volume of 500 mL?
To achieve a 1:5 dilution, how much dilution water is needed for a total sample volume of 500 mL?
What is the chemical equation representing the oxidation of organic compounds in the BOD method?
What is the chemical equation representing the oxidation of organic compounds in the BOD method?
Which compound is NOT involved in the nitrification process during BOD testing?
Which compound is NOT involved in the nitrification process during BOD testing?
What is the correct dilution ratio for the second dilution in Set 1?
What is the correct dilution ratio for the second dilution in Set 1?
Which of the following compounds is NOT listed as a component of the dilution water?
Which of the following compounds is NOT listed as a component of the dilution water?
What is the adjusted pH value of the dilution water?
What is the adjusted pH value of the dilution water?
If the BOD7 is calculated to be 80% of BODtotal, what percentage is BOD5 based on BODtotal?
If the BOD7 is calculated to be 80% of BODtotal, what percentage is BOD5 based on BODtotal?
In the BOD calculation formula, what does the variable 'a' represent?
In the BOD calculation formula, what does the variable 'a' represent?
What does the variable 'M' represent in the BOD calculation formula?
What does the variable 'M' represent in the BOD calculation formula?
What is the purpose of incubating the sample for 7 days?
What is the purpose of incubating the sample for 7 days?
During the BOD5 calculation, which of the following values is subtracted?
During the BOD5 calculation, which of the following values is subtracted?
At what dissolved oxygen levels does iron typically occur as Fe3+?
At what dissolved oxygen levels does iron typically occur as Fe3+?
What is the impact of elevated concentrations of iron and manganese in water?
What is the impact of elevated concentrations of iron and manganese in water?
Which of the following statements is true regarding the solubility of metals in groundwater?
Which of the following statements is true regarding the solubility of metals in groundwater?
What concentration of dissolved oxygen is likely to keep iron present in the more soluble Fe2+ form?
What concentration of dissolved oxygen is likely to keep iron present in the more soluble Fe2+ form?
What health concern is likely associated with elevated aluminum levels in drinking water?
What health concern is likely associated with elevated aluminum levels in drinking water?
During which stage is sodium thiosulphate used for titration?
During which stage is sodium thiosulphate used for titration?
What is one of the most noticeable effects of high concentrations of iron in water?
What is one of the most noticeable effects of high concentrations of iron in water?
How does groundwater interact with metals found in soils and rocks?
How does groundwater interact with metals found in soils and rocks?
Flashcards
Biochemical Oxygen Demand (BOD)
Biochemical Oxygen Demand (BOD)
The amount of dissolved oxygen needed by aerobic microorganisms to break down organic matter in water.
BOD Method Stages
BOD Method Stages
A two-stage process: Stage I oxidizes simple and complex organic compounds, while Stage II (after 6-10 days) includes nitrification (oxidizing nitrogen compounds).
Winkler Method
Winkler Method
A technique used to measure dissolved oxygen levels in water samples.
Sample Dilution
Sample Dilution
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Incubation (BOD)
Incubation (BOD)
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Nitrification
Nitrification
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Dissolved Oxygen (DO)
Dissolved Oxygen (DO)
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Dilution Factor
Dilution Factor
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BOD5
BOD5
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BOD7
BOD7
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DW
DW
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Dilution Factor
Dilution Factor
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BOD Calculation
BOD Calculation
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Dilution 1
Dilution 1
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Dilution 2
Dilution 2
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Sample Volume (incubation)
Sample Volume (incubation)
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Iron forms
Iron forms
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Dissolved Oxygen (DO) impact on Fe
Dissolved Oxygen (DO) impact on Fe
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Water quality impact of metals
Water quality impact of metals
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Metal source in groundwater
Metal source in groundwater
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Sample volume for analysis
Sample volume for analysis
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Sample volume for calculations
Sample volume for calculations
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Sample dilution in research
Sample dilution in research
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Parameters measured in sets
Parameters measured in sets
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Groundwater Iron Oxidation
Groundwater Iron Oxidation
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Iron Detection Method
Iron Detection Method
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Manganese Detection Method
Manganese Detection Method
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Maximum Permissible Iron Limit (drinking water)
Maximum Permissible Iron Limit (drinking water)
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Sulphates Detection
Sulphates Detection
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Study Notes
Biochemical Oxygen Demand (BOD)
- BOD is the amount of dissolved oxygen needed by aerobic microorganisms to mineralize organic compounds in a water sample.
- The process can be represented by the equation: organic compounds + O₂ → CO₂ + H₂O + bacteria.
BOD Stages
- Stage I: Oxidation of simple organic compounds like proteins, sugars, and starches. Also oxidation of complex organic compounds like fats, cellulose, and pectins.
- Stage II (after 6-10 days): Nitrification - oxidation of nitrogen compounds (NH₄⁺ → NO₂⁻ → NO₃⁻) in aerobic conditions by autotrophic bacteria.
BOD Method
- Fill Winkler bottles: Fill them with samples according to a specific procedure.
- Determine initial dissolved oxygen: Measure dissolved oxygen in the samples using the Winkler method.
- Incubation: Leave a set of samples for 7 days, sealed properly.
- Determine final dissolved oxygen: Measure dissolved oxygen after incubation.
- Dilution: Use a graduated cylinder to prepare dilutions for analysis (e.g., 1:5 to 1:10). These dilutions take total sample volume and divide it into the desired ratio of sample (water) & dilution water.
- Calculations: Use the formula BOD = [(a-b) - (c-d)M] / 1000 to calculate BOD, where (a), (b), (c), and (d) are oxygen concentrations (in mg O₂/L) before and after incubation (for sample and dilution water respectively). M is the amount of dilution water mL contained in 1 L of sample and m is the mL amount of water in 1L of sample. The BOD₅ is typically calculated – the value at 5 days into the process.
Dilution Water (DW) Composition
- Phosphate buffer, magnesium sulphate, calcium chloride, and magnesium chloride.
- Saturated with oxygen (≈ 8 mg/L).
- Adjusted pH value of 7.2.
Iron (Fe) and Manganese (Mn)
- The solubility of Fe and Mn in groundwater is dependent on the oxygen concentration (more oxygen means less Fe, Mn, and thus less damage to pipes).
- Iron exists as Fe²⁺ (reduced) or Fe³⁺ (oxidized) depending on the oxygen level. At higher oxygen levels, iron becomes Fe³⁺ which can cause rusty color deposits.
- Manganese has similar oxidation states.
- These metal levels need to be kept at safe concentrations.
Methods for Metals
- Iron: Use a Nessler cylinder, hydroxylamine hydrochloride, acetate buffer, 1.10 phenanthroline, then use a spectrophotometer at 510 nm.
- Manganese: Use 100 mL of water sample, specific reagent mixture, ammonium persulfate, heat for a minute, then a spectrophotometer at 525 nm.
- Sulfates: Use 5.0 mL water sample, a "reagent", and a spectrophotometer at 430 nm.
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