Biochemical Oxygen Demand Quiz

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Questions and Answers

What is the oxidation product of dissolved iron when exposed to oxygen in groundwater?

  • Fe2O3
  • Fe(OH)2
  • Fe(OH)3 (correct)
  • Fe(HCO3)2

Which method is used to determine the concentration of manganese in water samples?

  • Direct measurement without any reagents
  • Spectrophotometry at 480 nm
  • Using a Nessler cylinder without any dilution
  • Heating and boiling the sample with ammonium persulfate (correct)

What is the maximum permissible value of iron in drinking water?

  • 50 μg/L
  • 200 μg/L (correct)
  • 100 μg/L
  • 250 mg/L

Which step is NOT part of the iron concentration measurement method using a Nessler cylinder?

<p>Heat the sample to boiling (A)</p> Signup and view all the answers

What is the wavelength used to check the concentration of sulphates in a water sample?

<p>430 nm (D)</p> Signup and view all the answers

What does the biochemical oxygen demand (BOD) measure?

<p>The amount of dissolved oxygen needed by microorganisms (B)</p> Signup and view all the answers

During which stage does nitrification occur in the BOD method?

<p>After 6-10 days of incubation (D)</p> Signup and view all the answers

What is the purpose of the Winkler method in the BOD process?

<p>To determine the dissolved oxygen concentration (D)</p> Signup and view all the answers

How many Winkler bottles are filled during the preparation of dilutions?

<p>Two bottles for each dilution prepared (D)</p> Signup and view all the answers

What organic compounds are oxidized in Stage I of the BOD method?

<p>Both simple and complex organic compounds (C)</p> Signup and view all the answers

To achieve a 1:5 dilution, how much dilution water is needed for a total sample volume of 500 mL?

<p>100 mL of water and 400 mL of dilution water (A)</p> Signup and view all the answers

What is the chemical equation representing the oxidation of organic compounds in the BOD method?

<p>organic compounds + O2 → CO2 + H2O + bacteria (B)</p> Signup and view all the answers

Which compound is NOT involved in the nitrification process during BOD testing?

<p>CO2 (carbon dioxide) (A)</p> Signup and view all the answers

What is the correct dilution ratio for the second dilution in Set 1?

<p>1:10 (D)</p> Signup and view all the answers

Which of the following compounds is NOT listed as a component of the dilution water?

<p>Sodium bicarbonate (D)</p> Signup and view all the answers

What is the adjusted pH value of the dilution water?

<p>7.2 (D)</p> Signup and view all the answers

If the BOD7 is calculated to be 80% of BODtotal, what percentage is BOD5 based on BODtotal?

<p>68% (A)</p> Signup and view all the answers

In the BOD calculation formula, what does the variable 'a' represent?

<p>Oxygen concentration before incubation (B)</p> Signup and view all the answers

What does the variable 'M' represent in the BOD calculation formula?

<p>Amount of dilution water contained in 1 L of the sample (C)</p> Signup and view all the answers

What is the purpose of incubating the sample for 7 days?

<p>To allow microorganisms to deplete the oxygen (B)</p> Signup and view all the answers

During the BOD5 calculation, which of the following values is subtracted?

<p>Oxygen concentration in the sample after incubation (A)</p> Signup and view all the answers

At what dissolved oxygen levels does iron typically occur as Fe3+?

<p>Greater than 1-2 mg/L (D)</p> Signup and view all the answers

What is the impact of elevated concentrations of iron and manganese in water?

<p>Clogging of pipelines (A)</p> Signup and view all the answers

Which of the following statements is true regarding the solubility of metals in groundwater?

<p>The solubility of metals is influenced by the amount of oxygen present. (D)</p> Signup and view all the answers

What concentration of dissolved oxygen is likely to keep iron present in the more soluble Fe2+ form?

<p>Below 1 mg/L (D)</p> Signup and view all the answers

What health concern is likely associated with elevated aluminum levels in drinking water?

<p>Alzheimer's disease (B)</p> Signup and view all the answers

During which stage is sodium thiosulphate used for titration?

<p>Before and after incubation (C)</p> Signup and view all the answers

What is one of the most noticeable effects of high concentrations of iron in water?

<p>An unpleasant metallic taste (D)</p> Signup and view all the answers

How does groundwater interact with metals found in soils and rocks?

<p>It can dissolve metals and release them. (C)</p> Signup and view all the answers

Flashcards

Biochemical Oxygen Demand (BOD)

The amount of dissolved oxygen needed by aerobic microorganisms to break down organic matter in water.

BOD Method Stages

A two-stage process: Stage I oxidizes simple and complex organic compounds, while Stage II (after 6-10 days) includes nitrification (oxidizing nitrogen compounds).

Winkler Method

A technique used to measure dissolved oxygen levels in water samples.

Sample Dilution

Reducing the concentration of a sample by adding a solvent, typically water.

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Incubation (BOD)

Allowing a water sample to sit and undergo biological processes (usually for 5 days).

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Nitrification

A process where ammonia is oxidized into nitrites then nitrates by specialized aerobic bacteria.

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Dissolved Oxygen (DO)

The amount of oxygen dissolved in a water sample, a key measurement in water quality.

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Dilution Factor

The ratio comparing the final volume of a diluted solution to the initial volume of the concentrated solution.

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BOD5

Biochemical Oxygen Demand after 5 days, a measure of the amount of dissolved oxygen consumed by microorganisms in a water sample.

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BOD7

Biochemical Oxygen Demand after 7 days, a measure of the amount of dissolved oxygen consumed by microorganisms in a water sample

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DW

Dilution Water containing phosphate buffer, magnesium sulphate, calcium chloride, magnesium chloride, microorganisms that are saturated with oxygen and adjusted to pH 7.2

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Dilution Factor

Ratio of dilution water to the original sample, commonly 1:5 or 1:10

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BOD Calculation

Calculated using oxygen concentration in the sample before and after incubation with the dilution water.

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Dilution 1

The first of 2 dilution steps in a BOD measurement with a dilution ratio of typically 1:5 or 1:10

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Dilution 2

Second dilution step in a BOD measurement, twice as large as the first, with a dilution ratio.

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Sample Volume (incubation)

500 ml is used for BOD procedure; 1000ml for calculations

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Iron forms

Iron exists in two forms: Fe2+ (ferrous) and Fe3+ (ferric).

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Dissolved Oxygen (DO) impact on Fe

High DO levels favor Fe3+, while low DO levels favor Fe2+.

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Water quality impact of metals

High concentrations of iron and manganese can affect taste, odor, and potentially cause pipeline blockage.

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Metal source in groundwater

Metals dissolve from soil, rocks, and minerals when groundwater contacts them.

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Sample volume for analysis

500 mL is often used when testing and analyzing chemical properties of a sample.

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Sample volume for calculations

1000 mL is often used to calculate the amount of a parameter or substance present in the sample.

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Sample dilution in research

The process of reducing the concentration of a sample by adding dilution water.

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Parameters measured in sets

Parameters measured, such as oxygen concentration, and amount of sodium thiosulfate used, can be measured (titrated) before and after incubation.

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Groundwater Iron Oxidation

Groundwater iron reacts with atmospheric oxygen, changing from Fe2+ to Fe3+ (oxidation), and forming rust-colored deposits.

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Iron Detection Method

Measures iron concentration by adding reagents (hydroxylamine hydrochloride, acetate buffer, 1.10 phenanthroline) to a water sample then measuring the absorbance at 510nm with a spectrophotometer.

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Manganese Detection Method

Measures manganese concentration by adding a special reagent (a mixture of HgSO4, HNO3, and AgNO3) to a water sample, heating, and measuring the absorbance at 525nm with a spectrophotometer.

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Maximum Permissible Iron Limit (drinking water)

The maximum acceptable concentration of iron in drinking water is 200 µg/L.

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Sulphates Detection

Detects sulphate concentration by adding a specific reagent ('A reagent') to a water sample, shaking and measuring the absorbance at 430nm using a spectrophotometer.

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Study Notes

Biochemical Oxygen Demand (BOD)

  • BOD is the amount of dissolved oxygen needed by aerobic microorganisms to mineralize organic compounds in a water sample.
  • The process can be represented by the equation: organic compounds + O₂ → CO₂ + H₂O + bacteria.

BOD Stages

  • Stage I: Oxidation of simple organic compounds like proteins, sugars, and starches. Also oxidation of complex organic compounds like fats, cellulose, and pectins.
  • Stage II (after 6-10 days): Nitrification - oxidation of nitrogen compounds (NH₄⁺ → NO₂⁻ → NO₃⁻) in aerobic conditions by autotrophic bacteria.

BOD Method

  • Fill Winkler bottles: Fill them with samples according to a specific procedure.
  • Determine initial dissolved oxygen: Measure dissolved oxygen in the samples using the Winkler method.
  • Incubation: Leave a set of samples for 7 days, sealed properly.
  • Determine final dissolved oxygen: Measure dissolved oxygen after incubation.
  • Dilution: Use a graduated cylinder to prepare dilutions for analysis (e.g., 1:5 to 1:10). These dilutions take total sample volume and divide it into the desired ratio of sample (water) & dilution water.
  • Calculations: Use the formula BOD = [(a-b) - (c-d)M] / 1000 to calculate BOD, where (a), (b), (c), and (d) are oxygen concentrations (in mg O₂/L) before and after incubation (for sample and dilution water respectively). M is the amount of dilution water mL contained in 1 L of sample and m is the mL amount of water in 1L of sample. The BOD₅ is typically calculated – the value at 5 days into the process.

Dilution Water (DW) Composition

  • Phosphate buffer, magnesium sulphate, calcium chloride, and magnesium chloride.
  • Saturated with oxygen (≈ 8 mg/L).
  • Adjusted pH value of 7.2.

Iron (Fe) and Manganese (Mn)

  • The solubility of Fe and Mn in groundwater is dependent on the oxygen concentration (more oxygen means less Fe, Mn, and thus less damage to pipes).
  • Iron exists as Fe²⁺ (reduced) or Fe³⁺ (oxidized) depending on the oxygen level. At higher oxygen levels, iron becomes Fe³⁺ which can cause rusty color deposits.
  • Manganese has similar oxidation states.
  • These metal levels need to be kept at safe concentrations.

Methods for Metals

  • Iron: Use a Nessler cylinder, hydroxylamine hydrochloride, acetate buffer, 1.10 phenanthroline, then use a spectrophotometer at 510 nm.
  • Manganese: Use 100 mL of water sample, specific reagent mixture, ammonium persulfate, heat for a minute, then a spectrophotometer at 525 nm.
  • Sulfates: Use 5.0 mL water sample, a "reagent", and a spectrophotometer at 430 nm.

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