Bioanalysis of Biomolecules: Structure, Function, and Interactions
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Questions and Answers

What type of molecules can be analyzed through Bioanalysis?

  • Proteins, lipids, nucleotides, and amino acids
  • Carbohydrates, DNA, triglycerides, and organic acids
  • Enzymes, antibodies, lipoproteins, and polysaccharides
  • Proteins, DNA, RNA, carbohydrates, lipids, and small molecules (correct)

Which type of bond connects amino acids in a protein molecule?

  • Ionic bond
  • Hydrogen bond
  • Covalent bond
  • Peptide bond (correct)

What is the major difference in spectroscopic behavior between alpha-helices and beta-sheets in proteins?

  • Secondary and tertiary structures
  • Max absorption wavelength and extinction coefficient (correct)
  • Chirality and solubility
  • Rotation around peptide bonds

Which term describes the tertiary structure of proteins as domains?

<p>Quaternary structure (D)</p> Signup and view all the answers

What type of groups are prosthetic groups in proteins?

<p>Tightly bound to a protein and essential for function (D)</p> Signup and view all the answers

What role do soluble cofactors play in protein function?

<p>They diffuse into the active center for catalysis (B)</p> Signup and view all the answers

What is the function of Competitive Radio-Immuno-Assay (RIA)?

<p>Measures antigen concentration (A)</p> Signup and view all the answers

How does Immunocytochemistry work?

<p>Localizes antigens in cells using labeled antibodies (D)</p> Signup and view all the answers

What is the purpose of Western Blot / Dot Blot techniques?

<p>Identify and quantify specific proteins in a sample (D)</p> Signup and view all the answers

How is ELISA (Enzyme-linked immunosorbent assay) readout typically measured?

<p>By colorimetric readout (A)</p> Signup and view all the answers

What is the main purpose of Immunoprecipitation (IP) / Co-Immunoprecipitation (Co-IP)?

<p>Isolate and identify protein-protein interactions (B)</p> Signup and view all the answers

In Competitive Radio-Immuno-Assay (RIA), what does reduced labeled antigen binding indicate?

<p>Higher sample antigen concentration (A)</p> Signup and view all the answers

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