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Bioanalysis of Biomolecules: Structure, Function, and Interactions

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Bioanalysis of Biomolecules: Structure, Function, and Interactions

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Questions and Answers

What type of molecules can be analyzed through Bioanalysis?

Proteins, lipids, nucleotides, and amino acids

Carbohydrates, DNA, triglycerides, and organic acids

Enzymes, antibodies, lipoproteins, and polysaccharides

Proteins, DNA, RNA, carbohydrates, lipids, and small molecules (correct)

Which type of bond connects amino acids in a protein molecule?

Ionic bond

Hydrogen bond

Covalent bond

Peptide bond (correct)

What is the major difference in spectroscopic behavior between alpha-helices and beta-sheets in proteins?

Secondary and tertiary structures

Max absorption wavelength and extinction coefficient (correct)

Chirality and solubility

Rotation around peptide bonds

Which term describes the tertiary structure of proteins as domains?

Quaternary structure Signup and view all the answers

What type of groups are prosthetic groups in proteins?

Tightly bound to a protein and essential for function Signup and view all the answers

What role do soluble cofactors play in protein function?

They diffuse into the active center for catalysis Signup and view all the answers

What is the function of Competitive Radio-Immuno-Assay (RIA)?

Measures antigen concentration Signup and view all the answers

How does Immunocytochemistry work?

Localizes antigens in cells using labeled antibodies Signup and view all the answers

What is the purpose of Western Blot / Dot Blot techniques?

Identify and quantify specific proteins in a sample Signup and view all the answers

How is ELISA (Enzyme-linked immunosorbent assay) readout typically measured?

By colorimetric readout Signup and view all the answers

What is the main purpose of Immunoprecipitation (IP) / Co-Immunoprecipitation (Co-IP)?

Isolate and identify protein-protein interactions Signup and view all the answers

In Competitive Radio-Immuno-Assay (RIA), what does reduced labeled antigen binding indicate?

Higher sample antigen concentration Signup and view all the answers