12 Questions
What type of molecules can be analyzed through Bioanalysis?
Proteins, DNA, RNA, carbohydrates, lipids, and small molecules
Which type of bond connects amino acids in a protein molecule?
Peptide bond
What is the major difference in spectroscopic behavior between alpha-helices and beta-sheets in proteins?
Max absorption wavelength and extinction coefficient
Which term describes the tertiary structure of proteins as domains?
Quaternary structure
What type of groups are prosthetic groups in proteins?
Tightly bound to a protein and essential for function
What role do soluble cofactors play in protein function?
They diffuse into the active center for catalysis
What is the function of Competitive Radio-Immuno-Assay (RIA)?
Measures antigen concentration
How does Immunocytochemistry work?
Localizes antigens in cells using labeled antibodies
What is the purpose of Western Blot / Dot Blot techniques?
Identify and quantify specific proteins in a sample
How is ELISA (Enzyme-linked immunosorbent assay) readout typically measured?
By colorimetric readout
What is the main purpose of Immunoprecipitation (IP) / Co-Immunoprecipitation (Co-IP)?
Isolate and identify protein-protein interactions
In Competitive Radio-Immuno-Assay (RIA), what does reduced labeled antigen binding indicate?
Higher sample antigen concentration
Learn about the determination of structure, function, interactions, and concentrations of biological molecules such as proteins, DNA, RNA, carbohydrates, lipids, and small molecules. Explore the diverse functions of proteins and their composition of amino acids.
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