12 Questions
What is the purpose of using an HA tag in molecular cloning?
To identify or isolate proteins using biochemical techniques like western blotting or immunofluorescence.
What is the role of a fluorescently labeled secondary antibody in immunofluorescence?
It binds to the primary antibody and is used to detect emitted fluorescence.
How does confocal microscopy produce sharp and detailed images?
By using a pinhole to selectively capture in-focus light.
What is the purpose of western blotting?
To detect specific proteins in a sample.
How were the C17.2 Neural cells established?
By retroviral transduction of the avian myc oncogene into cells of neonatal mouse cerebellum.
What induces fibrilization of tau in the context mentioned?
Arachidonic acid.
What are the two main pathological hallmarks of Alzheimer's disease?
Beta-amyloid plaques and neurofibrillary tangles of tau
In tauopathies, why are neurons dying?
Neurons are dying due to misfolded tau protein, leading to the formation of neurofibrillary tangles.
What is the suggested mechanism by which tau spreads through neighboring neurons in Alzheimer's disease and frontotemporal dementia?
The authors hypothesize that tau spreads through a 'prion-like' mechanism, where extracellular tau enters neighbor neurons and seeds misfolding of intracellular tau protein.
Where has tau been observed outside of neurons in Alzheimer's disease and frontotemporal dementia?
Tau has been observed in the cerebrospinal fluid (CSF) and in extracellular aggregates known as 'ghost tangles'.
What is the role of Yellow Fluorescent Protein (YFP) in the context of the study?
YFP is a genetic mutant of green fluorescent protein (GFP) derived from jellyfish Aequorea victoria.
What is the composition of the extracellular aggregates known as 'ghost tangles' in Alzheimer's disease and frontotemporal dementia?
Ghost tangles are made up of the microtubule binding region (MTBR) of full-length tau protein.
Test your knowledge on Alzheimer’s disease pathology focusing on extracellular beta-amyloid plaques and intracellular neurofibrillary tangles of tau. Explore the concept of tauopathies and the role of tau in neurodegeneration.
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