10 Questions
What is the unit of frequency?
Cycles/second or s-1 or Hertz (Hz)
What is the characteristic used to measure the distance between two waves of light?
Wavelength (λ)
What carries the energy in electromagnetic radiation?
Photons
Which property characterizes the number of waves per centimeter in electromagnetic radiation?
Wavenumber
What is the primary purpose of chromatography?
Separation, identification, and purification of components of a mixture
What is the formula to calculate energy carried by a photon?
E = hν
Which substances can be separated using chromatography?
Pigments, dyes, amino acids, vitamins, polymers
Who is credited with inventing and naming liquid chromatography?
Mikhail Tswett
What does the term 'chromatography' mean?
'Color Writing'
What is the definition of chromatography?
'A technique for separating mixtures into their components in order to analyze, identify, purify and/or quantify the mixture or components'
Study Notes
Instrumental Analysis
- In instrumental analysis, a physical property of a substance is measured to determine its chemical composition.
Goals of Analysis
- The goal of analysis is to provide information about the composition of the sample, which is called quantitative analysis.
Types of Analytical Methods
- There are three categories of analytical methods:
- Chemical methods
- Instrumental methods
- Chromatography methods
Types of Chemical Analysis
- There are two types of chemical analysis:
Qualitative Analysis
- Refers to identifying the product, yielding useful clues about the molecular or atomic species, structural features, or functional groups in the sample.
Quantitative Analysis
- Refers to determining the purity of the product, providing numerical data corresponding to the concentration of the analyte.
- This type of analysis is done using instruments.
Choosing an Instrument
- The choice of instrument depends on the property being measured.
Learn about the fundamental principles of using instruments to measure the physical properties of substances for the purpose of determining their chemical composition. Explore the different types of analytical methods and their applications in biochemical, inorganic, organic, and physical analyses.
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