Banana and Plantain Propagation: PIF Technique
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Questions and Answers

What is the primary challenge hindering increased banana and plantain production, as indicated in the content?

  • A lack of suitable land for cultivation in various ecosystems.
  • The high costs associated with exporting bananas and plantains.
  • The extended growth cycle of over two years resulting from the use of poor-quality suckers. (correct)
  • Insufficient demand for bananas and plantains in the intertropical zone.

Compared to in vitro cultivation, what is a key advantage of the PIF technique for banana and plantain propagation?

  • PIF requires more specialized equipment and technical expertise than _in vitro_ cultivation.
  • PIF offers a higher success rate in producing disease-resistant plantlets.
  • PIF is generally more effective for research purposes, as it allows for precise genetic manipulation.
  • PIF is typically less expensive and faster, making it more accessible to small-scale farmers. (correct)

In the PIF technique, what step is crucial for inducing sprouts from axillary buds?

  • Subjecting the suckers to a period of intense cold.
  • Applying chemical fertilizers directly to the corms.
  • Planting entire suckers without any modification.
  • Exposing axillary buds to high humidity after destroying primary buds. (correct)

What is the potential plantlet yield from one sucker using the PIF technique, based on the information provided?

<p>Between 10 and 50 plantlets, depending on the variety and farmer's experience. (A)</p> Signup and view all the answers

According to the content, which of the following is the MOST crucial consideration when constructing a propagator for PIF production?

<p>Ensuring easy access to the explants within the propagator. (A)</p> Signup and view all the answers

If a banana farmer observes signs of disease on their suckers, at which stage of the PIF process should they implement phytosanitary treatments, according to the listed steps?

<p>Immediately after peeling the bulbs (Stage 7). (C)</p> Signup and view all the answers

A farmer is planning to implement the PIF technique but has limited resources. Which initial investment would provide the most benefit for the success of the technique?

<p>Constructing a durable and accessible propagator. (A)</p> Signup and view all the answers

Assuming a plantain farmer follows all steps of the PIF production, what is the MOST critical factor that determines the number of plantlets they can obtain from a single sucker?

<p>The variety of plantain and the farmer's experience with the PIF technique. (C)</p> Signup and view all the answers

Why is it crucial to peel up to 2 mm above the knot when preparing a bulb for propagation?

<p>To avoid damaging the sensitive tissues at the knot, which could hinder growth. (C)</p> Signup and view all the answers

What is the primary reason for cutting the pseudostem back to 1 to 2 cm above the stem’s last visible knot?

<p>To concentrate the plant’s energy on developing new roots rather than maintaining the old stem. (C)</p> Signup and view all the answers

What is the significance of making a right-angled crosscut on the bud at the starting point of each leaf during bulb preparation?

<p>It prevents the leaves from tightly wrapping the bulb, allowing for better air circulation and reduced risk of rot. (A)</p> Signup and view all the answers

What is the recommended procedure for phytosanitary treatment of peeled stems to eliminate fungus, insects, and nematodes?

<p>Soak the peeled stem in a mix of fungicide and insecticide, then dry in a dry, shady place. (C)</p> Signup and view all the answers

Why is it important to contact a local agricultural service provider for phytosanitary treatment information rather than relying solely on general guidelines?

<p>Specific products and instructions vary, and local providers can offer tailored advice appropriate for regional conditions and regulations. (C)</p> Signup and view all the answers

What is the recommended dilution and application method for neem oil as an organic insecticide-fungicide, based on the provided information?

<p>Dilute 2 cl of neem oil in one liter of water and spray using a vaporizer. (B)</p> Signup and view all the answers

When preparing garlic as a natural insecticide-fungicide, what is the correct procedure regarding dilution after the crushed bulbs have been steeped?

<p>Use the filtered solution without dilution, spraying it once it is cold. (A)</p> Signup and view all the answers

If a new banana plantation is established, and the farmer wants to optimize growth potential, which environmental conditions should the farmer ensure are consistently maintained?

<p>Humid tropical environment with well-drained, moist soil, temperatures between 24-29°C, and rainfall between 1800-2500 mm. (A)</p> Signup and view all the answers

A grower notices a minor fungal infection on their bulbs after peeling. Considering both commercial and organic options, what initial treatment approach would be MOST prudent?

<p>Start with a diluted application of neem oil, monitoring the bulb's response before considering stronger treatments. (C)</p> Signup and view all the answers

A researcher is studying the genetic lineage of a banana specimen. If the banana is identified as a hybrid of Musa acuminata and Musa balbisiana, what are the implications for its original species classification?

<p>It suggests a reclassification, as the original classifications of <em>Musa sapientum</em> and <em>Musa paradisiaca</em> are now understood to be hybrids of the two wild species. (D)</p> Signup and view all the answers

A food scientist aims to develop a new product using plantains, focusing on their nutritional benefits. Considering the established nutritional profile of plantains, which health benefit can be most effectively advertised?

<p>Rich in vitamin B6 and vitamin C, contributing to immune function and energy metabolism. (D)</p> Signup and view all the answers

An entrepreneur is planning to establish a sustainable enterprise using plantain plants. Which product would best utilize the plant's characteristics while also capitalizing on the concept of sustainability?

<p>Manufacturing ropes, paper and matting from plantain fibers, promoting eco-friendly alternatives. (D)</p> Signup and view all the answers

A botanist discovers a plant that appears to be a cross between a banana and another fruit-bearing plant. Which characteristic would definitively classify it as being closely related to the plantain rather than a typical banana?

<p>The unripe fruit is green and transitions to dark as it ripens. (D)</p> Signup and view all the answers

A farmer observes that their banana plants, which were previously healthy, are now producing smaller, less flavorful fruit. They consult an agricultural expert. What is the most likely recommendation the expert will make based on the optimal growing conditions for bananas?

<p>Ensure the plants are in a location with consistent temperatures between 24-29°C and adequate rainfall of 1800–2500 mm. (B)</p> Signup and view all the answers

In a comparative study of global food sources, a researcher analyzes the cultivation and consumption patterns of bananas and plantains. What conclusion would accurately describe the global significance of these crops?

<p>Bananas are globally valued for their flavor and availability, while plantains serve as a primary staple in African, Caribbean, and Latino diets. (B)</p> Signup and view all the answers

A structural engineer is inspired by the architecture of plantain plants to design sustainable buildings. Which aspect of the plantain plant's structure could be adapted into building design features?

<p>The conical pseudostem formed by leaf sheaths can inspire modular, self-supporting architectural columns. (A)</p> Signup and view all the answers

What is the primary reason for placing newly replanted plantlets in a shade during acclimatization?

<p>To facilitate the gradual adjustment to environmental conditions. (D)</p> Signup and view all the answers

Why is it important to ensure water can easily drain through the bags when replanting plantlets?

<p>To reduce the risk of root rot and maintain soil aeration. (C)</p> Signup and view all the answers

What is the potential consequence of planting a plantlet too deeply in the soil during replanting?

<p>Delayed growth initiation. (D)</p> Signup and view all the answers

What is the recommended temperature range for plantlet acclimatization after replanting, and why is maintaining it important?

<p>25-27°C, to facilitate acclimatization. (C)</p> Signup and view all the answers

Considering agriculture as a business, which of the following strategies would be LEAST effective for maximizing net return?

<p>Minimizing labor costs by delaying essential farm operations. (D)</p> Signup and view all the answers

What is the significance of regularly weeding the nursery where plantlets are growing?

<p>To reduce competition for resources. (D)</p> Signup and view all the answers

A farmer is deciding between two varieties of plantain. Variety A yields larger fruit but is susceptible to a common local disease. Variety B is disease-resistant but produces smaller fruit. Which approach best integrates agricultural science and art to maximize profit?

<p>Planting a mix of both varieties, combined with implementing targeted disease management strategies for Variety A. (A)</p> Signup and view all the answers

What is the optimal temperature for growing plantain, and what happens to its growth rate outside of this temperature range?

<p>28°C; growth gradually slows down from 28 to 20°C and becomes negligible around 16-18°C. (D)</p> Signup and view all the answers

How does understanding the 'art' of agriculture enhance a farmer's ability to adapt to unpredictable environmental changes, such as unusual rainfall patterns?

<p>It enables the farmer to make informed decisions based on experience and observation, adjusting practices to suit the specific conditions. (A)</p> Signup and view all the answers

How does shading affect the growth of plantain, and why is it important to manage the light conditions in a plantation?

<p>Shade accelerates height growth, making it important to adjust density based on the cultivar. (A)</p> Signup and view all the answers

What specific characteristic of polyethylene bags makes them suitable for replanting plantlets, and which size is highly recommended?

<p>Black and perforated; 17 cm x 24 cm. (B)</p> Signup and view all the answers

If a farmer's primary goal is to ensure the long-term productivity and health of their land, which of the following actions would be MOST aligned with the aims of sustainable agriculture?

<p>Implementing crop rotation and conservation tillage practices to improve soil structure and reduce erosion. (D)</p> Signup and view all the answers

In the context of plantain and banana cultivation, which of the following actions represents the MOST effective application of 'mental skill' in agriculture?

<p>Selecting a specific cultivar and cropping system based on regional climate data and historical yield performance. (C)</p> Signup and view all the answers

Given the characteristics of plants in the Musaceae family, what is the MOST critical factor to consider when selecting a site for a new plantain farm?

<p>Access to rich, well-drained soil and sufficient sunlight or partial shade. (C)</p> Signup and view all the answers

A farmer observes that their plantain crop is producing smaller fruits than expected, despite consistent watering and fertilization. Which of the following is the MOST strategic approach to diagnose the underlying issue?

<p>Consulting with a local agricultural extension agent to analyze soil samples, assess plant health, and identify potential nutrient deficiencies or pest problems. (B)</p> Signup and view all the answers

Compared to bananas, plantains have a significantly higher starch content, necessitating cooking before consumption. How does this difference in composition MOST directly influence cultivation and post-harvest handling practices?

<p>Plantains are typically harvested at a later stage of maturity compared to bananas. (B)</p> Signup and view all the answers

When creating an insecticidal spray using hot peppers, what is the purpose of shaking the mixture in a sealed container?

<p>To ensure even distribution of capsaicin throughout the water. (D)</p> Signup and view all the answers

Why is it recommended to make a right-angled crosscut in the center of the explant during propagation?

<p>To stimulate the development of multiple shoots by disrupting apical dominance. (C)</p> Signup and view all the answers

What is the primary reason for delaying watering of the explants until 24-30 hours after they are placed in the propagator?

<p>To prevent overhydration and potential rotting of the explants. (B)</p> Signup and view all the answers

Reactivation involves making a new right-angled crosscut in the pseudostem of the plantlet. What is the main purpose of this step?

<p>To stimulate further shoot production from the plantlet. (A)</p> Signup and view all the answers

Why is reactivation not recommended for beginners in plantlet propagation?

<p>It demands precise timing and a high level of experience to be successful. (A)</p> Signup and view all the answers

What is the critical factor in determining the number of explants that can be placed per square meter in the propagator?

<p>The size of the individual explants. (B)</p> Signup and view all the answers

In the context of using ashes as an insect repellent, what is the most likely reason for mixing the ashes with soapy water, urine, or milk?

<p>To improve the adhesion of the ashes to the plant surface, increasing their effectiveness. (C)</p> Signup and view all the answers

Why is it important to use a sharp knife to trim the explant surface, leaving only 2-3 mm of the pseudostem remaining?

<p>To minimize the risk of introducing pathogens into the explant. (A)</p> Signup and view all the answers

Flashcards

Agriculture

The art, science, and business of growing crops and livestock from Earth's natural resources for economic gain.

Farming

Producing food, animal feed, and industrial materials from the land.

Physical Skill (Agriculture)

Skill in performing farm operations efficiently, such as handling tools and applying inputs.

Mental Skill (Agriculture)

Ability to make informed decisions based on experience, like crop selection and timing.

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Agriculture as a Science

Using modern scientific technologies (breeding, protection, economics) to maximize yield and profit.

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Agriculture as a Business

Managing land, labor, water, and capital to maximize net return through production.

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Aim of Agriculture

To increase land productivity while preventing deterioration and misuse.

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Plantain

A starchy fruit, similar to bananas but larger, and typically cooked before eating due to higher starch content.

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Banana and Plantain Origin

Hybrids from Musa acuminata and Musa balbisiana.

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Banana Scientific Name & Climate

Musa sapientum, thrives in humid, tropical climates with 1800–2500 mm rainfall, optimum temperature is 24–29°C.

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Banana Value and Distribution

Valued for flavour, nutrition, and year-round availability; grown worldwide.

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Plantain Scientific Name & Use

Musa paradisiaca. Fruits turn yellow/dark when ripe. It is a staple food in African, Caribbean, and Latino diets.

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Plantain Nutritional Value

Excellent source of vitamin B6 and vitamin C.

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Plantain Climate Preference

Warm, humid tropical and subtropical climates.

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Plantain Plant Structure

Gigantic herbs from rhizomes, 3–10 meters tall, with a pseudostem.

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Plantain Fruit Characteristics

Fruits are green to brown-yellow, larger than bananas, arranged in hands of 10-20.

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In vitro Cultivation

A cultivation method used in research centers to produce healthy plant materials, but it is more expensive and slower than PIF.

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PIF Technique

A macro-propagation technique that uses plantlets from stem fragments to produce healthy planting materials. It is cheaper and faster than in vitro cultivation.

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Process of Macro-propagation (PIF)

Destroying the primary buds of suckers or corm fragments and exposing axillary buds to high humidity to induce sprouts.

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Success of PIF Among Farmers

Relatively easy to produce large numbers of plantlets quickly, with one sucker yielding 10-50 plantlets.

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Propagator (Seedbed)

A structure made from wood, plastic, or cement used to house and protect explants during the initial stages of growth. It must allow easy access to the explants.

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Making a Propagator

The first stage in PIF production, involving the construction of a propagator (seedbed) to house explants.

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Importance of Banana and Plantain

They are important crops in many countries of the intertropical zone, adapting to various ecosystems and offering great potential in food production.

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Major Problem in Banana/Plantain Production

Lack of healthy planting material, stemming from parasite influence and poor-quality suckers, extending the growth cycle.

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Capsicum Fruit Pesticide

A natural pesticide made from hot peppers.

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Ash-Based Pesticide

Using ashes suspended in liquid to protect plants.

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Explant Propagation

The stage of placing prepared explants in a controlled environment to encourage growth.

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Explant Crosscut

Cutting the explant to promote efficient growth.

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Sawdust in Propagation

A layer that retains moisture.

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Plantlet Reactivation

Re-cutting plantlets to promote further growth.

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Taking Cuttings

Removing young plants from the explant for individual growth.

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Cutting Implement

Young plants removed with a scalpel, razor blade, or knife.

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Knot (in bulb)

The light-colored band that attaches each leaf sheath to the bulb.

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Bulb Peeling

Removing outer layers of the bulb to expose the stem, typically removing 3-5 layers.

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Pseudostem Cutting

Cut back to 1 to 2 cm above the stem’s last visible knot.

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Phytosanitary Treatment

Treatment to eliminate fungus, insects, and nematodes, often involving soaking in fungicide and insecticide.

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Peeled Stem Treatment

Soaking peeled bulbs in a mix of fungicide and insecticide, then drying in a shady place.

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Neem Oil

A natural insecticide from the neem tree, diluted in water and sprayed.

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Garlic Solution

Crushed bulbs soaked in boiling water, filtered, and sprayed as a natural fungicide-insecticide.

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Natural Insecticides/Fungicides

Substances derived from nature that kill or repel insects and fungi.

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Plantlet Replanting

Replanting the plantlet with all its roots into a perforated polyethylene bag for further growth.

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Rootless Plantlet Care

Plantlets without roots should be replanted in sawdust for ten days before being transferred to bags.

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Ideal Bag Compost

A compost mixture, sometimes including sand or coffee husks, ensuring good drainage.

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Plantlet Planting Depth

Plant only the bulb in the soil. Planting too deep delays growth.

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Plantlet Acclimatization

Ensuring they receive regular watering, are kept in shade, and maintained at a temperature of 25-27°C.

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Nursery Plant Care

Regular weeding and application of insecticide in bags or propagators.

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Optimal Plantain Temperature

The optimal temperature is 28°C. Growth slows from 28 to 20°C and is negligible around 16-18°C

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Shade Management

Determining shade density based on cultivar to optimize light exposure.

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Study Notes

  • Agriculture is the art, science, and business of producing crops and livestock for economic purposes and involves the production of food, fodder, and industrial materials.
  • Agriculture requires physical and mental skills, using scientific principles to maximize yield and profit, and is commercialized through mechanization to maximize net return.
  • The aim of agriculture is to cause the land to produce more abundantly, and at the same time, to protect it from deterioration and misuse.

About Bananas and Plantains

  • Both bananas and plantains are fruit crops of the genus Musa and family Musaceae.

  • Musaceae are evergreen, palm-like perennials which require sun or partial shade and well-drained soil.

  • Plantains look like bananas but are bigger and usually cooked due to a higher starch content.

  • Bananas and plantains are hybrids of Musa acuminata and Musa balbisiana.

  • Cavendish banana's scientific name is Musa sapientum and it grows in humid, tropical climates with 1800-2500 mm rainfall; optimum temperature is 24–29°C.

  • Bananas are native to Bangladesh, Malaysia, etc. but grown in India, Taiwan, Equator, Coast Rica, Panama, Mexico, Ivory coast, Columbia, Nigeria and Guatemala.

  • The scientific name of the commonly grown plantain is Musa paradisiaca.

  • Plantain fruits are green when unripe but turn yellow and then dark as they ripen.

  • Plantain is a staple food in African, Caribbean, and Latino diets, and is fried, boiled, or processed into flour.

  • Plantains are an excellent source of vitamins B6 and C.

  • Plantain fibers are used to make ropes, paper, and matting.

  • Plantains are native to Malaya and Philippines but are now being grown in different countries in Africa.

  • Plantains grow well in warm and humid tropical and subtropical climates.

  • Plantain plants are gigantic herbs from underground stems or rhizomes, that have a conical false "trunk" and fleshy root system and leaves grow up to 2.7m long and 60cm wide and their height is generally 3-10 meters.

Problems Facing Banana and Plantain Farmers

  • A major problem is the lack of healthy planting material stemming from parasites, leading to poor-quality suckers and a growth cycle of over two years.
  • In vitro cultivation and macro-propagation provide healthy plant materials. Macro-propagation (PIF) is cheaper and faster than in vitro culture.
  • PIF is the acronyms of “Plants Issues de Fragments de Tiges” (French) which means “Plants derived from Stem Fragments".
  • PIF involves destroying primary buds and exposing axillary buds to high humidity to induce sprouts.
  • PIF is relatively easy to produce large numbers of plantlets.
  • One sucker can produce between 10 and 50 plantlets via PIF, depending on the variety and experience.

Stages in PIF Production

  • Making a propagator
  • Making a greenhouse
  • Making a shade house
  • Selecting suckers
  • Cleaning and trimming of bulbs
  • Peeling of bulbs
  • Phytosanitary treatments of peeled stem before propagation
  • Propagation of the explants
  • Reactivation
  • Taking cuttings
  • Replanting in bags and acclimatisation in shade houses
  • Plant care

Making A Propagator

  • Propagators, or seedbeds, are made from wood, plastic or cement.
  • Propagator Depth is 25 to 50 cm.
  • Bottom is sealed to prevent direct contact between the substrate and the ground below.
  • A 10 cm layer of fine sand or gravel can be added to the bottom, or use perforated polythene.
  • The seedbed is filled with a 20 cm deep layer of light-colored sawdust, but avoid colored wood.
  • Recommended dimensions are 1 meter wide with variable length, which allows easy access to explants

Making a Greenhouse

  • The greenhouse is built around the propagator.
  • Build a framework 80 cm to 120 cm above the seed flat.
  • It is best to make two slopes to facilitate runoff in case of rain.
  • The propagator should be hermetically sealed with strong, transparent polythene.

Making a Shade House

  • The material used will depend on availability and resources (palm fronds, straw, netting, etc.).
  • It should be 1.5 – 2 metres high, and should reduce sunlight on the seedlings by about 50 percent.

Selecting Suckers

  • Selection is key: remove a healthy sucker from the base of a fully-grown cultivar
  • Suckers should have narrow, sword-shaped leaves, with a pseudostem of 5 to 40 cm in height, and the bulb free from disease.

Cleaning and Trimming of Bulbs

  • Involves cleaning the bulb with a sharp knife or machete.
  • The top of the bulb and all the roots should be removed, up to 3 to 5 mm.
  • Once trimming is completed, the trimmed and cleansed bulb should be totally white

Peeling of Bulb

  • Crucial to the success
  • Remove the leaf sheaths one by one.
  • Note that the base of the plantain plant has a conical pseudostem formed by the leaf sheaths of its spirally arranged, long, thin leaves.
  • At the level of the bulb, there is a light-colored band (the color depends on the variety) that attaches each leaf sheath to the bulb (the knot).
  • Each leaf sheath has a knot. Peel up to 2 mm above the knot.
  • Remove the sheaths one by one, generally 3 to 5 layers.
  • The pseudostem should be cut back to 1 to 2 cm above the stem's last visible knot.
  • Make a right-angled crosscut on the bud at the starting point of each leaf

Phytosanitary Treatments of Peeled Stem Before Propagation

  • Eliminate fungus, insects and nematodes by soaking the peeled stem in a mix of fungicide and insecticide, then drying in a shady place for 48-72 hours.
  • The local agricultural service provider should be contacted for specific information, as there are different products.
  • Peeling involves removing leaf sheaths down to the knot, cutting the pseudostem back, and making a right-angled crosscut on the bud.
  • Examples
  • The peeled bulbs can be soaked in a mix of 100 g of Callidium 50EC and Ridomil Plus in 40 liters of water.
  • Table 1: lists some commercial pesticides (CALLIDIUM-RIDOMIL PLUS, SARPAGRUM, CAIMAN ROUGE).
  • Table 2: lists some natural substances used as insecticide and fungicide(Neem, Garlic, Hot peppers, Insecticidal plants (e.g. Mahogany)).

Propagation of the Explants

  • Place explants in the propagator, trim the surface, make a right-angled crosscut, and let stand for 30-60 minutes.
  • Position explants side by side with the crosscut facing up, cover with a 2-3 cm layer of light-colored sawdust, and water thoroughly 24-30 hours later.

Reactivation

  • Two weeks later, explants will have multiple shoots that should be thumb size before reactivation.
  • Remove plantlets 2 mm above the node, make a new right-angled crosscut, but note reactivation isn't necessary.

Taking Cuttings

  • Taking cuttings occurs 30 to 40 days after propagation.
  • Carefully remove young plants with 3 to 5 leaves with a sharp knife.
  • Take cuttings occurs 30 to 40 days after propagation where young plants are harvested.

Replanting in Bags and Acclimatization in Shade Houses

  • Replant the plantlet with all its roots in a black, perforated polyethylene bag.
  • Separated plantlets without roots should be replanted in the propagator sawdust for ten days before transferring to bags, using 17 cm x 24 cm bags.
  • Fill bags with rich compost, mixed with sand or coffee husks, and water the evening before replanting. Bags should be thoroughly soaked when beginning replanting.

Plant Care

  • Weed the nursery regularly.
  • Insecticide can be directly applied to bags or propagator, and foliar fertilization can be applied if needed.
  • Plantlets are ready six to ten weeks after taking cuttings to be planted and can be transferred to the field.

Agronomic Requirements for Growing Plantain

  • Optimal temperature is 28°C, with growth slowing down below 20°C and stopping around 16-18°C.
  • Shade accelerates height growth and depends on cultivar selected.
  • Plantain needs around 200 mm of water per month and is very sensitive to strong wind.
  • Fallow land can be used, but the yield will depend on what was previously grown and the duration of the fallow.
  • Plantain grows best in deep soil that is well drained and rich in organic matter.

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Description

This quiz covers the PIF (Plantlet from Fragment) technique for banana and plantain propagation. It examines challenges in production, advantages of PIF over in vitro methods, critical steps for sprout induction, and potential plantlet yield. It also covers crucial considerations for propagator construction and disease management.

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