Bacteriology Culture Media Quiz

Culture Requirements for Isolation of Specific Bacteria

• Haemophilus influenzae thrives on Chocolate agar, which contains Factors V (NAD+) and X (hematin), along with Vancomycin to inhibit other organisms.
• Neisseria gonorrhoeae and Neisseria meningitidis grow on Thayer-Martin agar, specifically designed to favor Neisseria while suppressing gram-positive bacteria using Trimethoprim and Colistin. Fungal growth is inhibited by Nystatin.
• Bordetella pertussis can be isolated using Bordet-Gengou agar or Regan-Lowe medium, both supplemented with potato extract, charcoal, blood, and antibiotics to support growth.
• Corynebacterium diphtheriae is cultured using Tellurite agar or Löffler medium, which are special media suited for its isolation.
• Mycobacterium tuberculosis requires Löwenstein-Jensen medium or Middlebrook medium for culture, and can also be rapidly cultivated in automated broth cultures.
• Mycoplasma pneumoniae growth is facilitated by Eaton agar, with a specific requirement for cholesterol in the media.
• Lactose-fermenting Enterics are isolated on MacConkey agar, where lactose fermentation results in acid production, enabling identification.

Culture Requirements for Isolation of Specific Bacteria

• Haemophilus influenzae thrives on Chocolate agar, which contains Factors V (NAD+) and X (hematin), along with Vancomycin to inhibit other organisms.
• Neisseria gonorrhoeae and Neisseria meningitidis grow on Thayer-Martin agar, specifically designed to favor Neisseria while suppressing gram-positive bacteria using Trimethoprim and Colistin. Fungal growth is inhibited by Nystatin.
• Bordetella pertussis can be isolated using Bordet-Gengou agar or Regan-Lowe medium, both supplemented with potato extract, charcoal, blood, and antibiotics to support growth.
• Corynebacterium diphtheriae is cultured using Tellurite agar or Löffler medium, which are special media suited for its isolation.
• Mycobacterium tuberculosis requires Löwenstein-Jensen medium or Middlebrook medium for culture, and can also be rapidly cultivated in automated broth cultures.
• Mycoplasma pneumoniae growth is facilitated by Eaton agar, with a specific requirement for cholesterol in the media.
• Lactose-fermenting Enterics are isolated on MacConkey agar, where lactose fermentation results in acid production, enabling identification.

Culture Requirements for Isolation of Specific Bacteria

• Haemophilus influenzae thrives on Chocolate agar, which contains Factors V (NAD+) and X (hematin), along with Vancomycin to inhibit other organisms.
• Neisseria gonorrhoeae and Neisseria meningitidis grow on Thayer-Martin agar, specifically designed to favor Neisseria while suppressing gram-positive bacteria using Trimethoprim and Colistin. Fungal growth is inhibited by Nystatin.
• Bordetella pertussis can be isolated using Bordet-Gengou agar or Regan-Lowe medium, both supplemented with potato extract, charcoal, blood, and antibiotics to support growth.
• Corynebacterium diphtheriae is cultured using Tellurite agar or Löffler medium, which are special media suited for its isolation.
• Mycobacterium tuberculosis requires Löwenstein-Jensen medium or Middlebrook medium for culture, and can also be rapidly cultivated in automated broth cultures.
• Mycoplasma pneumoniae growth is facilitated by Eaton agar, with a specific requirement for cholesterol in the media.
• Lactose-fermenting Enterics are isolated on MacConkey agar, where lactose fermentation results in acid production, enabling identification.

Culture Requirements for Isolation of Specific Bacteria

• Haemophilus influenzae thrives on Chocolate agar, which contains Factors V (NAD+) and X (hematin), along with Vancomycin to inhibit other organisms.
• Neisseria gonorrhoeae and Neisseria meningitidis grow on Thayer-Martin agar, specifically designed to favor Neisseria while suppressing gram-positive bacteria using Trimethoprim and Colistin. Fungal growth is inhibited by Nystatin.
• Bordetella pertussis can be isolated using Bordet-Gengou agar or Regan-Lowe medium, both supplemented with potato extract, charcoal, blood, and antibiotics to support growth.
• Corynebacterium diphtheriae is cultured using Tellurite agar or Löffler medium, which are special media suited for its isolation.
• Mycobacterium tuberculosis requires Löwenstein-Jensen medium or Middlebrook medium for culture, and can also be rapidly cultivated in automated broth cultures.
• Mycoplasma pneumoniae growth is facilitated by Eaton agar, with a specific requirement for cholesterol in the media.
• Lactose-fermenting Enterics are isolated on MacConkey agar, where lactose fermentation results in acid production, enabling identification.

Culture Requirements for Isolation of Specific Bacteria

• Haemophilus influenzae thrives on Chocolate agar, which contains Factors V (NAD+) and X (hematin), along with Vancomycin to inhibit other organisms.
• Neisseria gonorrhoeae and Neisseria meningitidis grow on Thayer-Martin agar, specifically designed to favor Neisseria while suppressing gram-positive bacteria using Trimethoprim and Colistin. Fungal growth is inhibited by Nystatin.
• Bordetella pertussis can be isolated using Bordet-Gengou agar or Regan-Lowe medium, both supplemented with potato extract, charcoal, blood, and antibiotics to support growth.
• Corynebacterium diphtheriae is cultured using Tellurite agar or Löffler medium, which are special media suited for its isolation.
• Mycobacterium tuberculosis requires Löwenstein-Jensen medium or Middlebrook medium for culture, and can also be rapidly cultivated in automated broth cultures.
• Mycoplasma pneumoniae growth is facilitated by Eaton agar, with a specific requirement for cholesterol in the media.
• Lactose-fermenting Enterics are isolated on MacConkey agar, where lactose fermentation results in acid production, enabling identification.

Culture Requirements for Isolation of Specific Bacteria

• Haemophilus influenzae thrives on Chocolate agar, which contains Factors V (NAD+) and X (hematin), along with Vancomycin to inhibit other organisms.
• Neisseria gonorrhoeae and Neisseria meningitidis grow on Thayer-Martin agar, specifically designed to favor Neisseria while suppressing gram-positive bacteria using Trimethoprim and Colistin. Fungal growth is inhibited by Nystatin.
• Bordetella pertussis can be isolated using Bordet-Gengou agar or Regan-Lowe medium, both supplemented with potato extract, charcoal, blood, and antibiotics to support growth.
• Corynebacterium diphtheriae is cultured using Tellurite agar or Löffler medium, which are special media suited for its isolation.
• Mycobacterium tuberculosis requires Löwenstein-Jensen medium or Middlebrook medium for culture, and can also be rapidly cultivated in automated broth cultures.
• Mycoplasma pneumoniae growth is facilitated by Eaton agar, with a specific requirement for cholesterol in the media.
• Lactose-fermenting Enterics are isolated on MacConkey agar, where lactose fermentation results in acid production, enabling identification.

Test your knowledge on the specific culture media used for isolating various bacterial pathogens, including Haemophilus influenzae and Neisseria species. This quiz provides insights into the composition and selective properties of different agar media. Perfect for microbiology students!