Clinical Bacteriology - MT 6320

Lysine is broken down to Cadaverine

Beta-galactosidase converts ONPG to galactose and o-nitrophenol

Bacteria can hydrolyze gelatin, causing liquification

Ornithine is broken down to Putrescine

Arginine breaks down through steps to Ornithine and then to Putrescine

Bacteria produces green color

Bacteria produces a brownish or purple color

Results showing a yellow color within a range of time

A type of bacteria that produces hydrogen sulfide.

A biochemical reaction where bacteria produce hydrogen sulfide.

A test to determine if an organism produces an enzyme called oxidase.

A test to identify bacteria that can break down urea.

A test determining if bacteria can reduce nitrate to nitrite.

A semi-solid medium for determining if an organism produces sulfur compounds.

A characteristic or pattern of bacterial growth (possibly in a certain medium).

Stab-streak method is used to culture bacteria.

A positive result for the beta-d-glucoronidase test indicates the presence of E.coli, producing an electric blue fluorescence under UV light.

A negative result for the beta-d-glucoronidase test is typically associated with other enterics like Klebsiella. These organisms do not produce the enzyme beta-d-glucoronidase, resulting in no fluorescence.

The Indole Test detects the presence of tryptophanase, an enzyme that breaks down tryptophan into indole. A positive result is indicated by a red ring forming in the broth after adding Kovac's reagent. This test helps differentiate bacteria based on their ability to break down tryptophan.

The Methyl Red Test determines if a bacterium uses the mixed acids pathway for fermenting glucose. A positive result is indicated by a red color in the broth after adding Methyl Red reagent. This test helps identify bacteria that produce a significant amount of acid from glucose fermentation.

The Voges-Proskauer Test (VP Test) detects the presence of acetoin (acetyl methylcarbinol) as a product of glucose fermentation by certain bacteria. A positive result is indicated by a red coloration in the broth after adding Barritt's reagent. This test differentiates bacteria based on their ability to produce acetoin.

The Citrate Utilization Test determines if a bacterium can utilize citrate as its sole carbon source. A positive result is indicated by a deep blue color in the Simmon's Citrate Agar. This test helps differentiate bacteria based on their ability to utilize citrate.

The IMVIC test is a series of four biochemical tests used to differentiate enterobacteria. The tests include the Indole, Methyl Red, Voges-Proskauer, and Citrate tests. The combination of results for these four tests can help identify specific types of bacteria.

Common enteric bacteria that are often differentiated with the IMVIC test include E. coli, Klebsiella, Enterobacter, Serratia, and Citrobacter. Their unique metabolic profiles and reactions to these tests help in their identification.