Bacterial Polymers and Biofilms Quiz
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Questions and Answers

Exopolysaccharides (EPS) secreted by bacteria are characterized by their:

  • Role in active transport of water molecules across the bacterial cell membrane, ensuring hydration.
  • Ability to create a hydrophobic environment, promoting closer contact with host cells by displacing water. (correct)
  • Primary structure consisting of amino acid polymers that enhance protein synthesis.
  • Hydrophilic nature, facilitating nutrient uptake from the surrounding environment.
  • The capsule formed by exocellular polysaccharides (CPS) around a bacterium primarily functions to:

  • Enhance bacterial motility by reducing friction against host tissues.
  • Increase the surface area for nutrient absorption from the external environment.
  • Provide a protective barrier against the ingress of harmful chemicals, including immune molecules and antibiotics. (correct)
  • Facilitate the exchange of genetic material with neighboring bacterial cells.
  • Biofilms are complex structures composed of various biological polymers. Which of the following is a key component of biofilms, beyond exocellular polysaccharides?

  • Deoxyribonucleic acid (DNA) and proteins, contributing to the biofilm's architecture and function. (correct)
  • Lipopolysaccharides (LPS) that contribute to the biofilm's structural rigidity.
  • Peptidoglycans forming a mesh-like network throughout the biofilm matrix.
  • Phospholipids that create a hydrophobic barrier, preventing water penetration into the biofilm.
  • The 'complex architecture' of biofilms, allowing fluid flow, is most critical for:

    <p>Facilitating efficient nutrient delivery to bacteria within the biofilm and removal of metabolic waste products. (B)</p> Signup and view all the answers

    Pathogens actively scavenge serine from the host environment primarily because serine:

    <p>Depletes the host's available energy resources, particularly affecting immune cell function. (C)</p> Signup and view all the answers

    Lipopolysaccharide (LPS), known as endotoxin, triggers a powerful inflammatory response by directly interacting with:

    <p>Toll Like Receptor 4 (TLR4) on immune cells, initiating a cascade of inflammatory signaling events. (D)</p> Signup and view all the answers

    Superantigens induce a 'cytokine storm' due to their unique mechanism of action, which involves:

    <p>Non-specific activation of a broad population of T-cells by bridging Class II MHC on APCs with the TCR. (A)</p> Signup and view all the answers

    The symptoms associated with a 'cytokine storm' induced by superantigens, such as fever, nausea, vomiting, and shock, are primarily a result of:

    <p>Widespread systemic inflammation and physiological dysregulation caused by excessive cytokine release. (C)</p> Signup and view all the answers

    Which of the following combinations of vector and associated disease is INCORRECT?

    <p>Tick - Bubonic plague (D)</p> Signup and view all the answers

    Beyond adapting to varying pH and temperature, what other critical environmental factor must a pathogen navigate for successful transmission?

    <p>Host cell immune responses (B)</p> Signup and view all the answers

    Which adhesion factor is primarily responsible for the initial attachment of a pathogen to host cells?

    <p>Fimbriae/pili (A)</p> Signup and view all the answers

    What is the primary function of Type IV Pili, besides their role in adhesion?

    <p>DNA transfer (C)</p> Signup and view all the answers

    What role do exopolysaccharides (EPS) play in pathogen adhesion?

    <p>They facilitate the formation of biofilms, aiding in attachment. (B)</p> Signup and view all the answers

    Which of the following is NOT a biological product capable of transmitting pathogens?

    <p>Antibiotics (A)</p> Signup and view all the answers

    Which of these environmental factors does a pathogen NOT need to adapt to for successful transmission?

    <p>Barometric pressure (A)</p> Signup and view all the answers

    Apart from motility, what other role might flagella play in pathogen transmission?

    <p>Adhesion to host cells (D)</p> Signup and view all the answers

    What is the primary function of the "Toxin Complex" as described in the text?

    <p>To facilitate the direct transfer of toxic proteins into host cells. (A)</p> Signup and view all the answers

    Which of the following diseases is NOT transmitted through direct contact?

    <p>Cholera (D)</p> Signup and view all the answers

    Which type of toxin is produced by multi-enzyme "factories" within the bacterial cell?

    <p>Small drug-like toxins, such as polyketides (B)</p> Signup and view all the answers

    What is a key characteristic of specialized toxin delivery systems in bacterial pathogens?

    <p>They can directly inject toxins into host cells upon contact. (C)</p> Signup and view all the answers

    What is a key distinction between droplet transmission and airborne transmission of diseases?

    <p>Droplet transmission involves larger particles that travel shorter distances, while airborne transmission involves smaller particles that can travel longer distances. (C)</p> Signup and view all the answers

    What types of bacteria utilize PVCs for toxin delivery?

    <p>Mainly Gram-negative bacteria (D)</p> Signup and view all the answers

    Which of the following is an example of an environmental reservoir for a zoonotic disease?

    <p>Soil containing anthrax spores (A)</p> Signup and view all the answers

    How does the transmission of rabies differ from the transmission of anthrax?

    <p>Rabies primarily spreads through animal bites, while anthrax can spread through contact with contaminated animal products or spores. (B)</p> Signup and view all the answers

    What is the role of siderophores produced by some bacteria?

    <p>Scavenging iron from the host environment (B)</p> Signup and view all the answers

    What is TSST-1 and what does it cause?

    <p>A protein toxin, causing Toxic Shock Syndrome. (B)</p> Signup and view all the answers

    What role did Dr. John Snow play in understanding disease transmission?

    <p>He identified contaminated water as the source of a cholera outbreak. (C)</p> Signup and view all the answers

    What distinguishes the 'toxin adaptor' from the 'injector' in the 'Toxin Complex'?

    <p>The adaptor links the toxin to the injector machinery. (B)</p> Signup and view all the answers

    Which of these could serve as a fomite?

    <p>A contaminated doorknob (B)</p> Signup and view all the answers

    Besides toxins, what other substances can be produced by 'multi-enzyme factories'?

    <p>Immune inhibitors and antibiotics (A)</p> Signup and view all the answers

    What is a common characteristic of diseases spread through droplet transmission?

    <p>They often involve respiratory symptoms. (D)</p> Signup and view all the answers

    How does vertical transmission differ from other forms of direct contact transmission?

    <p>Vertical transmission occurs from mother to offspring, while other forms occur between any two individuals. (B)</p> Signup and view all the answers

    Which combination of factors contributes most significantly to a pathogen's overall virulence?

    <p>Survival in the host and tissue damage capacity (C)</p> Signup and view all the answers

    A bacterium possesses membrane-associated virulence factors. What role are these factors most likely to play in the infection process?

    <p>Promoting adhesion to host cells and evading immune responses (C)</p> Signup and view all the answers

    What distinguishes pathogenicity from virulence in the context of bacterial infections?

    <p>Pathogenicity refers to the ability to cause disease, while virulence refers to the severity of the disease. (C)</p> Signup and view all the answers

    Which stage of bacterial infection is primarily facilitated by secretory virulence factors?

    <p>Invasion of host tissues and immune evasion (B)</p> Signup and view all the answers

    How do cytosolic virulence factors contribute to a bacterium's ability to cause disease?

    <p>By enabling the bacterium to adapt to the intracellular environment (B)</p> Signup and view all the answers

    What is the primary role of a bacterial reservoir in the context of disease transmission?

    <p>To provide a site for the pathogen to replicate and maintain its population (B)</p> Signup and view all the answers

    A new bacterial pathogen is discovered that exhibits high transmissibility but low virulence. Which scenario is most likely to occur?

    <p>The pathogen will spread widely but cause mostly mild or asymptomatic infections. (C)</p> Signup and view all the answers

    In the context of bacterial pathogenesis, what does "tissue damage" specifically refer to?

    <p>The combined effects of bacterial toxins, enzymes, and other virulence factors on host cells and tissues (D)</p> Signup and view all the answers

    Flashcards

    Pathogen

    An organism that causes disease to its host.

    Pathogenicity

    The capacity to initiate an infectious disease.

    Virulence

    The capacity to cause disease and its severity.

    Transmissibility

    The ability to transmit from human-to-human or reservoir-to-human.

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    Infectivity

    The ability to breach host defenses.

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    Virulence Factors

    Proteins or molecules essential for a pathogen's pathogenicity.

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    Human Reservoirs

    Humans that transmit pathogens directly to others.

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    Animal Reservoirs

    Pathogens transmitted from animals, with humans as incidental hosts.

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    Zoonosis

    Transmission of diseases from animals to humans, e.g., rabies or plague.

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    Environmental Reservoirs

    Sources in the environment, such as plants or water, that harbor pathogens.

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    Cholera

    A disease caused by Vibrio cholerae from contaminated water, leading to severe diarrhea.

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    Direct Contact Transmission

    Microbes transferred via touching or direct body contact between individuals.

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    Indirect Contact Transmission

    Transmission involving contaminated objects (fomites) that carry pathogens.

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    Droplet Transmission

    Spread of pathogens through droplets in the air from coughing or sneezing.

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    Zoonosis Sources

    Pathogens from animals via bites, contaminated food, or animal products.

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    Legionnaires Disease

    A respiratory disease caused by Legionella bacteria, often linked to water supplies.

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    Invertebrate vector

    Organisms like ticks, mosquitos, and fleas that transmit pathogens.

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    Biological products

    Products derived from biological sources, including vaccines and blood products.

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    Pathogen adaptation

    The ability of pathogens to survive in varying conditions like pH and temperature.

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    Adhesion

    Process by which organisms attach to host tissues for colonization.

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    Attachment proteins

    Proteins like pili and fimbriae that help pathogens stick to host cells.

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    Fimbriae/Pili

    Short protein fibers aiding bacterial attachment to surfaces.

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    Exopolysaccharides (EPS)

    Generic surface structures that assist in attachment during colonization.

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    Type IV Pili

    Specialized pili used for DNA transfer and motility in bacteria.

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    EPSs

    Polymers secreted by bacteria into the environment, often hydrophobic.

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    Capsule

    A protective layer around bacteria formed by polysaccharides, preventing harmful ingress.

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    Phagocytosis Prevention

    Mechanism by which CPS prevents immune cells from engulfing bacteria.

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    Biofilms

    Complex communities of microbes that enhance survival on surfaces or tissues.

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    Antibiotics Resistance

    Biofilms form barriers that limit the penetration of antibiotics.

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    Active Combat

    Methods used by pathogens to evade or attack host defenses.

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    Superantigens

    Toxins that trigger excessive immune responses, leading to cytokine storms.

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    LPS (Endotoxin)

    A component triggering inflammation via Toll Like Receptor TLR4.

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    Toxin Complex

    A multi-protein injection machine used by pathogens to deliver toxins into host cells.

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    Non-Ribosomal Peptide Synthases

    Multi-enzyme complexes that produce small drug-like toxins and immune inhibitors.

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    Specialized Toxin Delivery Systems

    Systems in bacteria that secrete proteins or toxins directly into host cells, critical for bacterial virulence.

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    Gram-positive Bacteria

    Bacteria with thick cell walls, can secrete toxins and proteins to enter host cells.

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    Gram-negative Bacteria

    Bacteria with thinner cell walls that often have more complex toxin delivery systems.

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    Active Combat Toxins

    Toxins produced actively by pathogens to evade immune responses and cause damage.

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    Immune Inhibitors

    Molecules produced by pathogens to suppress the host's immune response.

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    Siderophores

    Small molecules produced by bacteria to scavenge iron from the host, essential for bacterial growth.

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    Study Notes

    Mechanisms of Bacterial Virulence

    • A pathogen is an organism that causes disease in a host.
    • Pathogenicity is the ability to initiate an infectious disease.
    • Virulence is the capacity to cause disease and the severity of the disease.
    • Transmissibility is the ability to spread from human to human or reservoir to human.
    • Survival is the ability to avoid destruction by the host's immune system, reproduce, and obtain nutrients within the host.
    • Infectivity is the capacity to overcome host defenses.
    • Virulence factors are the proteins or molecules produced by an organism to promote its pathogenicity.

    Virulence Factors Classification

    • Cytosolic factors: Changes in metabolic rate, physiological functions, and morphological structures.
    • Membrane-associated factors: Aid in adhesion to host tissues and cells, and in evading host immunity.
    • Secretory factors: Used to enter tissues and evade innate and adaptive immune responses.

    Steps to Bacterial Infection

    • Transmission: Exposure to pathogens.
    • Adherence: Binding to the skin or mucosa.
    • Invasion: Breaking through barriers.
    • Survival: Growth at original and distal sites, producing virulence factors.
    • Tissue damage: Toxicity and disease.

    Transmission Reservoirs

    • Human reservoirs: Person-to-person transmission (asymptomatic carriage).
      • Examples: COVID, HIV, Measles, Mumps, Streptococcal infection, Respiratory pathogens.
    • Animal reservoirs: Animal-to-animal or animal-to-human transmission.
      • Examples: Brucellosis (cows and pigs), Anthrax (sheep), Plague (rodents), Trichinellosis (swine), Rabies (bats, dogs).
    • Environmental reservoirs: Plants, soil, and water.
      • Examples: Cholera outbreaks (Vibrio Cholera in contaminated water), Legionnaires' disease (caused by water in cooling towers).

    Transmission Mechanisms

    • Direct contact: Direct body surface contact (skin-to-skin contact, sexual transmission, mother-to-neonate).
      • Examples: HIV, Staphylococcal infections, infectious mononucleosis, Gonorrhoea.
    • Indirect contact: Contact via contaminated objects (fomites).
      • Examples: Cholera, Salmonella, Listeriosis, Viral hepatitis.
    • Droplets: Generated via coughing, sneezing, and talking; carrying microorganisms.
      • Examples: Influenza, Pneumonia, Meningococcal infections, Pertussis, Anthrax spores, Tuberculosis, SARS-CoV-2, Varicella, Hantaviruses.
    • Zoonoses: Animal-to-human transmission.
      • Animal bites (Rabies, Hepatitis), Animal products (contaminated meat, animal hides), Invertebrate vector-borne (ticks, mosquitos, fleas) (Lyme's disease, Malaria, Yellow fever, Typhus, Plague).

    Portal of Entry and Exit

    • Various routes through which bacteria enter (Mucous membranes (epithelium), Skin damage, Vertical transmission (across placenta, through the gametes), Specialized entry).
    • The various routes through which bacteria exit the body(Respiratory tract, Gastrointestinal tract, Genitourinary tract, Skin, blood).
    • Environmental adaptation for pathogen transmission.
    • Key pathogens require adaptation to survive in differing environmental conditions (Temperature, pH, Oxygen levels, Host cell immune response, Nutrient availability).

    How Pathogens Achieve Colonization

    • Cell surface components: Adhesion, defense against immunity (e.g., pili, fimbriae, flagella, EPS, CPS (capsule)).
    • Active combat: Invasive enzymes (e.g., coagulase, kinases, hyaluronidase, collagenase), Nutrient acquisition, Toxins.
    • General strategies: Dealing with phagocytosis, Communication/Intelligence signals, Subverting apoptosis.
    • Adhesion: The process of bacteria attaching to tissues or cells, crucial for colonization
    • Adhesion factors: Proteins such as pili, fimbriae and flagella enable attachment. Specialized surface structures (EPS, CPS)
    • Structural components of bacteria: Can act as toxins eg. LPS

    Active Combat

    • Invasive enzymes: Coagulase, Kinases, Hyaluronidase, Collagenase, IgA proteases.
    • Toxins: Botulinum, Shiga toxin, Staphylococcal enterotoxin, differing LD50 values. Different toxin groups, and structures.
    • LPS (Lipopolysaccharide): Gram-negative bacteria's outer membrane component, triggering Toll-like receptor 4 (TLR4), leading to an inflammatory response and fever. Little inherent toxicity.
    • Exotoxins: Specific toxins damaging or manipulating the host. (Single polypeptide toxins, Multiple polypeptide toxin complexes, Small "drug-like" molecules, Toxins directly injected).

    Active Combat - Specialized Toxin Delivery Systems

    • Secretory systems in bacteria to deliver virulence factors to host cells.
    • Type I, II, V, III, IV, VI, VII secretory systems in Gram-positive and Gram-negative bacteria.
    • Specialized mechanisms for injecting toxins into host cells. (e.g. Toxin complex, Injector, Toxin adaptor, toxin).

    Biofilms

    • After initial attachment, bacteria form biofilms for enhanced survival.
    • Biofilms are colonies adhering to each other and forming on abiotic surfaces and host tissues.
    • Biofilms provide a barrier against host defenses, antibiotics, and immune factors.
    • Complex architecture allowing for nutrient flow and waste removal

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    Description

    Test your knowledge on exopolysaccharides, biofilms, and their roles in bacterial physiology and pathogenesis. This quiz covers the functions of capsules, the importance of biofilm architecture, and the effects of lipopolysaccharides and superantigens. Dive into the complex interactions that bacteria have with their environments!

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