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Questions and Answers
Exopolysaccharides (EPS) secreted by bacteria are characterized by their:
Exopolysaccharides (EPS) secreted by bacteria are characterized by their:
The capsule formed by exocellular polysaccharides (CPS) around a bacterium primarily functions to:
The capsule formed by exocellular polysaccharides (CPS) around a bacterium primarily functions to:
Biofilms are complex structures composed of various biological polymers. Which of the following is a key component of biofilms, beyond exocellular polysaccharides?
Biofilms are complex structures composed of various biological polymers. Which of the following is a key component of biofilms, beyond exocellular polysaccharides?
The 'complex architecture' of biofilms, allowing fluid flow, is most critical for:
The 'complex architecture' of biofilms, allowing fluid flow, is most critical for:
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Pathogens actively scavenge serine from the host environment primarily because serine:
Pathogens actively scavenge serine from the host environment primarily because serine:
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Lipopolysaccharide (LPS), known as endotoxin, triggers a powerful inflammatory response by directly interacting with:
Lipopolysaccharide (LPS), known as endotoxin, triggers a powerful inflammatory response by directly interacting with:
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Superantigens induce a 'cytokine storm' due to their unique mechanism of action, which involves:
Superantigens induce a 'cytokine storm' due to their unique mechanism of action, which involves:
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The symptoms associated with a 'cytokine storm' induced by superantigens, such as fever, nausea, vomiting, and shock, are primarily a result of:
The symptoms associated with a 'cytokine storm' induced by superantigens, such as fever, nausea, vomiting, and shock, are primarily a result of:
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Which of the following combinations of vector and associated disease is INCORRECT?
Which of the following combinations of vector and associated disease is INCORRECT?
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Beyond adapting to varying pH and temperature, what other critical environmental factor must a pathogen navigate for successful transmission?
Beyond adapting to varying pH and temperature, what other critical environmental factor must a pathogen navigate for successful transmission?
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Which adhesion factor is primarily responsible for the initial attachment of a pathogen to host cells?
Which adhesion factor is primarily responsible for the initial attachment of a pathogen to host cells?
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What is the primary function of Type IV Pili, besides their role in adhesion?
What is the primary function of Type IV Pili, besides their role in adhesion?
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What role do exopolysaccharides (EPS) play in pathogen adhesion?
What role do exopolysaccharides (EPS) play in pathogen adhesion?
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Which of the following is NOT a biological product capable of transmitting pathogens?
Which of the following is NOT a biological product capable of transmitting pathogens?
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Which of these environmental factors does a pathogen NOT need to adapt to for successful transmission?
Which of these environmental factors does a pathogen NOT need to adapt to for successful transmission?
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Apart from motility, what other role might flagella play in pathogen transmission?
Apart from motility, what other role might flagella play in pathogen transmission?
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What is the primary function of the "Toxin Complex" as described in the text?
What is the primary function of the "Toxin Complex" as described in the text?
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Which of the following diseases is NOT transmitted through direct contact?
Which of the following diseases is NOT transmitted through direct contact?
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Which type of toxin is produced by multi-enzyme "factories" within the bacterial cell?
Which type of toxin is produced by multi-enzyme "factories" within the bacterial cell?
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What is a key characteristic of specialized toxin delivery systems in bacterial pathogens?
What is a key characteristic of specialized toxin delivery systems in bacterial pathogens?
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What is a key distinction between droplet transmission and airborne transmission of diseases?
What is a key distinction between droplet transmission and airborne transmission of diseases?
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What types of bacteria utilize PVCs for toxin delivery?
What types of bacteria utilize PVCs for toxin delivery?
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Which of the following is an example of an environmental reservoir for a zoonotic disease?
Which of the following is an example of an environmental reservoir for a zoonotic disease?
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How does the transmission of rabies differ from the transmission of anthrax?
How does the transmission of rabies differ from the transmission of anthrax?
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What is the role of siderophores produced by some bacteria?
What is the role of siderophores produced by some bacteria?
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What is TSST-1 and what does it cause?
What is TSST-1 and what does it cause?
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What role did Dr. John Snow play in understanding disease transmission?
What role did Dr. John Snow play in understanding disease transmission?
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What distinguishes the 'toxin adaptor' from the 'injector' in the 'Toxin Complex'?
What distinguishes the 'toxin adaptor' from the 'injector' in the 'Toxin Complex'?
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Which of these could serve as a fomite?
Which of these could serve as a fomite?
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Besides toxins, what other substances can be produced by 'multi-enzyme factories'?
Besides toxins, what other substances can be produced by 'multi-enzyme factories'?
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What is a common characteristic of diseases spread through droplet transmission?
What is a common characteristic of diseases spread through droplet transmission?
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How does vertical transmission differ from other forms of direct contact transmission?
How does vertical transmission differ from other forms of direct contact transmission?
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Which combination of factors contributes most significantly to a pathogen's overall virulence?
Which combination of factors contributes most significantly to a pathogen's overall virulence?
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A bacterium possesses membrane-associated virulence factors. What role are these factors most likely to play in the infection process?
A bacterium possesses membrane-associated virulence factors. What role are these factors most likely to play in the infection process?
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What distinguishes pathogenicity from virulence in the context of bacterial infections?
What distinguishes pathogenicity from virulence in the context of bacterial infections?
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Which stage of bacterial infection is primarily facilitated by secretory virulence factors?
Which stage of bacterial infection is primarily facilitated by secretory virulence factors?
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How do cytosolic virulence factors contribute to a bacterium's ability to cause disease?
How do cytosolic virulence factors contribute to a bacterium's ability to cause disease?
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What is the primary role of a bacterial reservoir in the context of disease transmission?
What is the primary role of a bacterial reservoir in the context of disease transmission?
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A new bacterial pathogen is discovered that exhibits high transmissibility but low virulence. Which scenario is most likely to occur?
A new bacterial pathogen is discovered that exhibits high transmissibility but low virulence. Which scenario is most likely to occur?
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In the context of bacterial pathogenesis, what does "tissue damage" specifically refer to?
In the context of bacterial pathogenesis, what does "tissue damage" specifically refer to?
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Flashcards
Pathogen
Pathogen
An organism that causes disease to its host.
Pathogenicity
Pathogenicity
The capacity to initiate an infectious disease.
Virulence
Virulence
The capacity to cause disease and its severity.
Transmissibility
Transmissibility
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Infectivity
Infectivity
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Virulence Factors
Virulence Factors
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Human Reservoirs
Human Reservoirs
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Animal Reservoirs
Animal Reservoirs
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Zoonosis
Zoonosis
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Environmental Reservoirs
Environmental Reservoirs
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Cholera
Cholera
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Direct Contact Transmission
Direct Contact Transmission
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Indirect Contact Transmission
Indirect Contact Transmission
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Droplet Transmission
Droplet Transmission
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Zoonosis Sources
Zoonosis Sources
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Legionnaires Disease
Legionnaires Disease
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Invertebrate vector
Invertebrate vector
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Biological products
Biological products
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Pathogen adaptation
Pathogen adaptation
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Adhesion
Adhesion
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Attachment proteins
Attachment proteins
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Fimbriae/Pili
Fimbriae/Pili
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Exopolysaccharides (EPS)
Exopolysaccharides (EPS)
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Type IV Pili
Type IV Pili
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EPSs
EPSs
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Capsule
Capsule
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Phagocytosis Prevention
Phagocytosis Prevention
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Biofilms
Biofilms
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Antibiotics Resistance
Antibiotics Resistance
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Active Combat
Active Combat
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Superantigens
Superantigens
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LPS (Endotoxin)
LPS (Endotoxin)
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Toxin Complex
Toxin Complex
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Non-Ribosomal Peptide Synthases
Non-Ribosomal Peptide Synthases
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Specialized Toxin Delivery Systems
Specialized Toxin Delivery Systems
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Gram-positive Bacteria
Gram-positive Bacteria
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Gram-negative Bacteria
Gram-negative Bacteria
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Active Combat Toxins
Active Combat Toxins
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Immune Inhibitors
Immune Inhibitors
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Siderophores
Siderophores
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Study Notes
Mechanisms of Bacterial Virulence
- A pathogen is an organism that causes disease in a host.
- Pathogenicity is the ability to initiate an infectious disease.
- Virulence is the capacity to cause disease and the severity of the disease.
- Transmissibility is the ability to spread from human to human or reservoir to human.
- Survival is the ability to avoid destruction by the host's immune system, reproduce, and obtain nutrients within the host.
- Infectivity is the capacity to overcome host defenses.
- Virulence factors are the proteins or molecules produced by an organism to promote its pathogenicity.
Virulence Factors Classification
- Cytosolic factors: Changes in metabolic rate, physiological functions, and morphological structures.
- Membrane-associated factors: Aid in adhesion to host tissues and cells, and in evading host immunity.
- Secretory factors: Used to enter tissues and evade innate and adaptive immune responses.
Steps to Bacterial Infection
- Transmission: Exposure to pathogens.
- Adherence: Binding to the skin or mucosa.
- Invasion: Breaking through barriers.
- Survival: Growth at original and distal sites, producing virulence factors.
- Tissue damage: Toxicity and disease.
Transmission Reservoirs
- Human reservoirs: Person-to-person transmission (asymptomatic carriage).
- Examples: COVID, HIV, Measles, Mumps, Streptococcal infection, Respiratory pathogens.
- Animal reservoirs: Animal-to-animal or animal-to-human transmission.
- Examples: Brucellosis (cows and pigs), Anthrax (sheep), Plague (rodents), Trichinellosis (swine), Rabies (bats, dogs).
- Environmental reservoirs: Plants, soil, and water.
- Examples: Cholera outbreaks (Vibrio Cholera in contaminated water), Legionnaires' disease (caused by water in cooling towers).
Transmission Mechanisms
- Direct contact: Direct body surface contact (skin-to-skin contact, sexual transmission, mother-to-neonate).
- Examples: HIV, Staphylococcal infections, infectious mononucleosis, Gonorrhoea.
- Indirect contact: Contact via contaminated objects (fomites).
- Examples: Cholera, Salmonella, Listeriosis, Viral hepatitis.
- Droplets: Generated via coughing, sneezing, and talking; carrying microorganisms.
- Examples: Influenza, Pneumonia, Meningococcal infections, Pertussis, Anthrax spores, Tuberculosis, SARS-CoV-2, Varicella, Hantaviruses.
- Zoonoses: Animal-to-human transmission.
- Animal bites (Rabies, Hepatitis), Animal products (contaminated meat, animal hides), Invertebrate vector-borne (ticks, mosquitos, fleas) (Lyme's disease, Malaria, Yellow fever, Typhus, Plague).
Portal of Entry and Exit
- Various routes through which bacteria enter (Mucous membranes (epithelium), Skin damage, Vertical transmission (across placenta, through the gametes), Specialized entry).
- The various routes through which bacteria exit the body(Respiratory tract, Gastrointestinal tract, Genitourinary tract, Skin, blood).
- Environmental adaptation for pathogen transmission.
- Key pathogens require adaptation to survive in differing environmental conditions (Temperature, pH, Oxygen levels, Host cell immune response, Nutrient availability).
How Pathogens Achieve Colonization
- Cell surface components: Adhesion, defense against immunity (e.g., pili, fimbriae, flagella, EPS, CPS (capsule)).
- Active combat: Invasive enzymes (e.g., coagulase, kinases, hyaluronidase, collagenase), Nutrient acquisition, Toxins.
- General strategies: Dealing with phagocytosis, Communication/Intelligence signals, Subverting apoptosis.
- Adhesion: The process of bacteria attaching to tissues or cells, crucial for colonization
- Adhesion factors: Proteins such as pili, fimbriae and flagella enable attachment. Specialized surface structures (EPS, CPS)
- Structural components of bacteria: Can act as toxins eg. LPS
Active Combat
- Invasive enzymes: Coagulase, Kinases, Hyaluronidase, Collagenase, IgA proteases.
- Toxins: Botulinum, Shiga toxin, Staphylococcal enterotoxin, differing LD50 values. Different toxin groups, and structures.
- LPS (Lipopolysaccharide): Gram-negative bacteria's outer membrane component, triggering Toll-like receptor 4 (TLR4), leading to an inflammatory response and fever. Little inherent toxicity.
- Exotoxins: Specific toxins damaging or manipulating the host. (Single polypeptide toxins, Multiple polypeptide toxin complexes, Small "drug-like" molecules, Toxins directly injected).
Active Combat - Specialized Toxin Delivery Systems
- Secretory systems in bacteria to deliver virulence factors to host cells.
- Type I, II, V, III, IV, VI, VII secretory systems in Gram-positive and Gram-negative bacteria.
- Specialized mechanisms for injecting toxins into host cells. (e.g. Toxin complex, Injector, Toxin adaptor, toxin).
Biofilms
- After initial attachment, bacteria form biofilms for enhanced survival.
- Biofilms are colonies adhering to each other and forming on abiotic surfaces and host tissues.
- Biofilms provide a barrier against host defenses, antibiotics, and immune factors.
- Complex architecture allowing for nutrient flow and waste removal
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Description
Test your knowledge on exopolysaccharides, biofilms, and their roles in bacterial physiology and pathogenesis. This quiz covers the functions of capsules, the importance of biofilm architecture, and the effects of lipopolysaccharides and superantigens. Dive into the complex interactions that bacteria have with their environments!