Bacterial Metabolism and Respiration Quiz

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Questions and Answers

Which pathway is NOT utilized in anaerobic respiration?

  • Fermentation (correct)
  • Respiratory Chain
  • Glycolysis
  • Kreb’s Cycle

What is the final electron acceptor in aerobic respiration?

  • Nitrate (NO3-)
  • Sulfate (SO4-)
  • Organic compounds
  • Molecular oxygen (O2) (correct)

Which statement accurately describes fermentation?

  • It produces a small amount of ATP without oxygen. (correct)
  • It occurs in the presence of oxygen.
  • It uses only inorganic electron acceptors.
  • It completely oxidizes glucose.

Why does anaerobic respiration produce less ATP than aerobic respiration?

<p>It only utilizes part of the Kreb's cycle. (A)</p> Signup and view all the answers

In anaerobic respiration, what might serve as a final electron acceptor?

<p>Nitrate (NO3-) (B)</p> Signup and view all the answers

What is the main purpose of metabolism in bacteria?

<p>To generate energy and synthesize cell material (B)</p> Signup and view all the answers

Which process is associated with anabolism in bacterial metabolism?

<p>Building complex molecules from simple ones (B)</p> Signup and view all the answers

What distinguishes chemoorganotrophs among bacteria?

<p>They oxidize preformed organic molecules for energy (A)</p> Signup and view all the answers

Which transport mechanism do Gram-negative bacteria use to take in larger molecules?

<p>Preliminary digestion in the periplasmic space (B)</p> Signup and view all the answers

Which of the following is NOT a biochemical mechanism utilized by bacteria for metabolism?

<p>Photosynthesis (D)</p> Signup and view all the answers

Which of the following provides phylogenetic information on bacteria and archaea?

<p>Bergey’s Manual of Systematic Bacteriology (C)</p> Signup and view all the answers

What is the primary characteristic that differentiates bacteria from other organisms?

<p>Single-celled structure (D)</p> Signup and view all the answers

Which resource provides lists of species of known prokaryotes based on published articles?

<p>Approved Lists of Bacterial Names (B)</p> Signup and view all the answers

Which of the following does NOT specialize in the classification of fungi?

<p>International Committee on Taxonomy of Viruses (A)</p> Signup and view all the answers

What is the significance of differential staining in bacterial classification?

<p>It provides morphological differentiation. (D)</p> Signup and view all the answers

What type of organisms are bacteria classified as?

<p>Prokaryotes (A)</p> Signup and view all the answers

Which of the following statements about bacterial DNA is true?

<p>It is usually a single circular molecule. (C)</p> Signup and view all the answers

What distinguishes fungi from bacteria in terms of cellular structure?

<p>Fungi have a complex nuclear structure. (D)</p> Signup and view all the answers

Which type of organism is NOT considered a class of parasite?

<p>Fungi (D)</p> Signup and view all the answers

What characteristic of protozoa makes them particularly concerning in terms of infection?

<p>They can multiply within human hosts. (D)</p> Signup and view all the answers

What type of infection can fungi cause that is often mistaken for other illnesses?

<p>Lung infections (A)</p> Signup and view all the answers

Which of the following is a characteristic of ectoparasites?

<p>They are multicellular. (A)</p> Signup and view all the answers

What is true about helminths in their adult form?

<p>They cannot multiply in human hosts. (C)</p> Signup and view all the answers

What does microbiology primarily study?

<p>Small living organisms (C)</p> Signup and view all the answers

Which area is NOT included in the course sections of microbiology?

<p>Pathobiology (D)</p> Signup and view all the answers

Which of the following topics is specifically about microbial infections?

<p>Host-pathogen relationships (D)</p> Signup and view all the answers

Which type of living organisms is studied under Mycology?

<p>Fungi (B)</p> Signup and view all the answers

Which of the following is NOT a focus of laboratory diagnosis in microbiology?

<p>Study of human anatomy (A)</p> Signup and view all the answers

Which of the following best describes the content of Topic 6?

<p>Coccus classification and species (A)</p> Signup and view all the answers

What is the primary purpose of studying microbial physiology?

<p>Examining the functions and processes of microorganisms (D)</p> Signup and view all the answers

Which microbial group is NOT mentioned in the course topics?

<p>Yeasts (B)</p> Signup and view all the answers

What is the primary structural component of Gram-positive bacteria's cell wall?

<p>Thick peptidoglycan layer (C)</p> Signup and view all the answers

What distinguishes Gram-negative bacteria from Gram-positive bacteria?

<p>They have a thin peptidoglycan layer. (B)</p> Signup and view all the answers

Which of the following structures is essential for the structural integrity of Gram-negative bacteria?

<p>Lipopolysaccharides (A)</p> Signup and view all the answers

What role do teichoic acids play in Gram-positive bacteria?

<p>Uptake of Mg2+ by the cell. (A)</p> Signup and view all the answers

How does a capsule contribute to the virulence of bacteria?

<p>By protecting against the host immune system. (C)</p> Signup and view all the answers

What is the periplasm in Gram-negative bacteria?

<p>The space between the outer and inner membranes. (A)</p> Signup and view all the answers

What role do lipopolysaccharides (LPS) play in bacterial ecology?

<p>They assist in surface adhesion. (D)</p> Signup and view all the answers

What is the primary function of the capsule in bacteria?

<p>Protecting against environmental threats. (B)</p> Signup and view all the answers

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What is the term for the sum of biochemical reactions required for energy generation in bacteria?

<p>Metabolism (C)</p> Signup and view all the answers

Which of the following processes is primarily associated with catabolism in bacterial metabolism?

<p>Aerobic respiration (D)</p> Signup and view all the answers

Which mechanism allows bacteria to transport larger molecules into the cell after preliminary digestion?

<p>Periplasmic digestion (C)</p> Signup and view all the answers

What are the components of metabolism that are critical for energy synthesis?

<p>Anabolism and catabolism (C)</p> Signup and view all the answers

Which of the following topics includes the study of viruses?

<p>Virology (A)</p> Signup and view all the answers

What is the primary focus of bacteriology within the course?

<p>Bacterial classification and characteristics (A)</p> Signup and view all the answers

Which topic addresses the host-pathogen interactions during infections?

<p>Host-pathogen relationships in microbial infections (D)</p> Signup and view all the answers

Which section of microbiology would involve the study of eukaryotic parasites?

<p>Parasitology (A)</p> Signup and view all the answers

What does the term 'micro' in microbiology refer to?

<p>Small living organisms (D)</p> Signup and view all the answers

Which topic would likely cover bacterial structure and classification techniques?

<p>Basic principles of medical microbiology (D)</p> Signup and view all the answers

Which of the following topics does NOT include mycology?

<p>Clinical Virology (A)</p> Signup and view all the answers

Which of the following best outlines the course sections in microbiology discussed?

<p>Bacteriology, Virology, Mycology, and Parasitology (A)</p> Signup and view all the answers

What does the term 'species' primarily refer to in taxonomy?

<p>The fundamental unit of classification representing a specific type of organism (C)</p> Signup and view all the answers

Which of the following statements about the binomial system of nomenclature is true?

<p>Both genus and species names are italicized or underlined. (D)</p> Signup and view all the answers

What defines a 'strain' in taxonomy?

<p>A subgroup within a species distinguished by specific characteristics. (D)</p> Signup and view all the answers

Which of these domains is NOT part of the three-domain system of classification?

<p>Fungi (C)</p> Signup and view all the answers

What percentage of similar rRNA do organisms within a species generally share?

<p>97% (A)</p> Signup and view all the answers

Which resource is specifically designed to provide phylogenetic information on bacteria and archaea?

<p>Bergey’s Manual of Systematic Bacteriology (C)</p> Signup and view all the answers

What characteristic distinguishes bacteria from other organisms in terms of cell structure?

<p>Absence of membrane-bound organelles (D)</p> Signup and view all the answers

What is primarily used in the naming of bacterial species based on published articles?

<p>Approved Lists of Bacterial Names (A)</p> Signup and view all the answers

Which genus name is derived from the name of a disease?

<p>Klebsiella (A)</p> Signup and view all the answers

Which method is commonly used for identifying bacteria and archaea through biochemical tests and morphology?

<p>Bergey’s Manual of Determinative Bacteriology (D)</p> Signup and view all the answers

What describes a virus in its extracellular state?

<p>Single nucleic acid surrounded by protein coat (D)</p> Signup and view all the answers

Which hypothesis suggests that viruses may have originated as fragments of larger nucleic acids?

<p>The fugitive nucleic acid hypothesis (B)</p> Signup and view all the answers

What is the primary structural feature of a virion?

<p>A protein coat surrounding nucleic acid (D)</p> Signup and view all the answers

How are viruses classified based on their nucleic acid?

<p>DNA or RNA viruses but not both (D)</p> Signup and view all the answers

What determines whether a virus is classified as enveloped or naked?

<p>Lipid presence around the nucleocapsid (C)</p> Signup and view all the answers

What characteristic is NOT used for the classification of viruses?

<p>Size of viral particles (A)</p> Signup and view all the answers

Which aspect of viruses remains uncertain regarding their origins?

<p>Whether they have common ancestors (C)</p> Signup and view all the answers

Which type of viruses contain a protein coat along with a lipid layer?

<p>Enveloped viruses (C)</p> Signup and view all the answers

What method did Louis Pasteur invent to prevent spoilage in consumable liquids?

<p>Pasteurization (B)</p> Signup and view all the answers

Which substance did Sir Joseph Lister introduce to sterilize surgical instruments?

<p>Phenol (B)</p> Signup and view all the answers

What is the first postulate of Koch's Postulates regarding microorganisms?

<p>The microorganism must be found in abundance in all diseased organisms. (A)</p> Signup and view all the answers

What significant contribution did Robert Koch make to microbiology?

<p>Proof of the Germ Theory (D)</p> Signup and view all the answers

What did Dmitri Ivanovski's work primarily contribute to?

<p>Foundations of Virology (D)</p> Signup and view all the answers

What was the purpose of the hand washing and chlorine disinfection implemented by an early practitioner?

<p>To reduce post-surgical infections (B)</p> Signup and view all the answers

Which of the following conditions must be met according to Koch's Postulates?

<p>The microorganism must be reproducibly isolated and identified. (A)</p> Signup and view all the answers

What role does phenol play in antiseptic surgery as introduced by Sir Joseph Lister?

<p>To sterilize surgical instruments (B)</p> Signup and view all the answers

Which of the following topics addresses relationships between hosts and pathogens during infections?

<p>Host-pathogen relationships in microbial infections (A)</p> Signup and view all the answers

Which area of study includes the interactions and classifications of viruses?

<p>Clinical Virology (D)</p> Signup and view all the answers

Which topic would likely cover the classification and characteristics of Gram-negative Bacilli?

<p>Gram negative Bacilli (C)</p> Signup and view all the answers

What best describes the range of organisms studied in mycology?

<p>Living organisms classified as fungi (B)</p> Signup and view all the answers

Which of the following principles is essential in laboratory diagnosis in microbiology?

<p>Microbial growth and control (A)</p> Signup and view all the answers

Which topic encompasses the study of frequent infections by parasitic agents?

<p>Parasitology (A)</p> Signup and view all the answers

Which course section does NOT focus on microbiology’s basic principles?

<p>Plant pathology (C)</p> Signup and view all the answers

Which statement accurately depicts the scope of microbiology?

<p>It covers living organisms too small for the naked eye. (B)</p> Signup and view all the answers

What process involves the building of complex molecules from simple ones and requires energy?

<p>Anabolism (C)</p> Signup and view all the answers

Which nutritional classification describes bacteria that obtain energy by oxidizing organic molecules from their environment?

<p>Chemoorganotrophs (B)</p> Signup and view all the answers

Which of the following is NOT a biochemical mechanism utilized by bacteria to generate energy?

<p>Photosynthesis (A)</p> Signup and view all the answers

What type of transport allows bacteria to uptake nutrients like amino acids across their cell membrane?

<p>Facilitated diffusion (B)</p> Signup and view all the answers

Which is the primary biological function of catabolism in bacterial metabolism?

<p>To generate energy (A)</p> Signup and view all the answers

Which process produces the least amount of ATP during energy production?

<p>Fermentation (A), Anaerobic Respiration (D)</p> Signup and view all the answers

What is a characteristic of anaerobic respiration compared to aerobic respiration?

<p>It operates through the same pathways as aerobic respiration. (D)</p> Signup and view all the answers

During fermentation, what is typically used as a terminal electron acceptor?

<p>Organic compounds (D)</p> Signup and view all the answers

Which statement is true regarding both aerobic and anaerobic respiration?

<p>They utilize glycolysis as a common pathway. (D)</p> Signup and view all the answers

Which of the following best describes why anaerobic respiration produces less ATP than aerobic respiration?

<p>Only part of the respiratory chain operates. (B)</p> Signup and view all the answers

What shape characterizes bacterial morphology when referred to as 'coccus'?

<p>Round shape (C)</p> Signup and view all the answers

Which of the following is NOT an extracellular structure of bacteria?

<p>Ribosome (D)</p> Signup and view all the answers

Which component is the primary structural element of the bacterial cell wall?

<p>Peptidoglycan (A)</p> Signup and view all the answers

In terms of locomotion, what is the primary role of flagella in bacteria?

<p>Facilitating chemotaxis (C)</p> Signup and view all the answers

Which bacterial shape is typically characterized by a curved or comma-like structure?

<p>Vibrio (C)</p> Signup and view all the answers

What is one of the main functional purposes of pili in bacteria?

<p>Attachment to surfaces (B)</p> Signup and view all the answers

Which of the following examples does NOT represent a pathogenic bacteria related to its morphology?

<p>Escherichia coli (B)</p> Signup and view all the answers

What type of metabolism is linked with the structural characteristics of bacteria?

<p>Catabolism (D)</p> Signup and view all the answers

Which statement correctly describes fungi?

<p>Fungi can be found in a variety of environments, including human skin. (B)</p> Signup and view all the answers

What distinguishes helminths from protozoa?

<p>Helminths are larger and can be seen with the naked eye in their adult stages. (A)</p> Signup and view all the answers

What role do ectoparasites play in relation to their host?

<p>They attach or burrow into the skin for extended periods. (C)</p> Signup and view all the answers

Which characteristic is true regarding bacteria?

<p>Bacteria often carry extra-chromosomal DNA in plasmids. (A)</p> Signup and view all the answers

Which of the following is a common symptom of fungal lung diseases?

<p>Symptoms resembling the flu (A)</p> Signup and view all the answers

Why are protozoa particularly concerning in terms of infection?

<p>They can multiply within the human host. (D)</p> Signup and view all the answers

Which differentiates fungal infections from other types of infections?

<p>Fungal infections can often be mistaken for viral infections. (A)</p> Signup and view all the answers

What is the main requirement for a virus to replicate?

<p>It must replicate within host cells. (D)</p> Signup and view all the answers

Which structural component surrounds the nucleic acid in a virus?

<p>Capsid (B)</p> Signup and view all the answers

Which hypothesis suggests that viruses could originate from pieces of nucleic acid that became independent?

<p>Fugitive nucleic acid hypothesis (C)</p> Signup and view all the answers

What distinguishes enveloped viruses from naked viruses?

<p>Presence of a lipid coat (D)</p> Signup and view all the answers

Which of the following is a characteristic feature of viruses?

<p>They are obligate intracellular parasites. (C)</p> Signup and view all the answers

Which factor is NOT considered in the classification of viruses?

<p>Color of the virus (D)</p> Signup and view all the answers

What type of nucleic acid can be present in virions?

<p>Both DNA and RNA (D)</p> Signup and view all the answers

What is the term for the virus particle that is metabolically inert outside of living cells?

<p>Virion (A)</p> Signup and view all the answers

Flashcards

Medical Microbiology

The study of microorganisms, especially those that cause disease in humans.

Microbial growth

The process by which microbes increase in number.

Antimicrobial agents

Substances that kill or inhibit the growth of microbes.

Host-pathogen relationship

The interaction between a host organism (like a human) and a pathogen (a disease-causing microbe).

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Diagnostic microbiology

The branch of microbiology that specializes in identifying and classifying microorganisms.

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Bacteriology

The branch of microbiology that deals with bacteria.

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Virology

The branch of microbiology that deals with viruses.

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Microbiology

The study of tiny living things (microbes).

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Bacteria characteristics

Bacteria are prokaryotes with a single, circular DNA molecule (chromosome) and are smaller than eukaryotic cells.

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Bacterial infections

Some bacteria cause various human infections like syphilis, pharyngitis, and anthrax.

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Fungi characteristics

Fungi are eukaryotes found in soil, plants, indoors, and on skin. They can cause diseases in humans.

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Fungal diseases

Fungal diseases can range from mild skin rashes to serious illnesses like fungal meningitis, and are often similar to other illnesses like flu or tuberculosis.

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Parasite definition

A parasite is an organism that lives on or in a host, obtaining its food from the host, often causing disease.

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Protozoa

Microscopic, single-celled organisms that can be parasitic causing infections from a single organism.

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Helminths

Large, multicellular organisms (often visible) that are parasitic

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Ectoparasites

Parasites that live on the surface of the host, like ticks or lice.

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Bacteria: Cell Structure

Bacteria are single-celled organisms with a simple structure, lacking a nucleus and membrane-bound organelles.

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Bergey's Manual of Determinative Bacteriology

A resource for identifying bacteria and archaea based on their morphology, staining properties, and biochemical characteristics.

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Bergey's Manual of Systematic Bacteriology

A guide for classifying bacteria and archaea based on their genetic relationships, using rRNA sequencing.

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Approved Lists of Bacterial Names

A database of officially recognized bacterial species, based on published scientific articles.

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Mycobank

A database for identifying and classifying fungi.

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Bacterial Nutrient Uptake

Bacteria take in small molecules like amino acids, sugars, and peptides through their cell wall. The cell membrane then uses facilitated diffusion or active transport to move these nutrients into the cytoplasm.

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Periplasmic Space Role

Gram-negative bacteria have an extra space between the cell wall and cell membrane called the periplasmic space. This space is used for preliminary digestion of larger molecules before they enter the cell.

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What is Metabolism?

Metabolism is the sum of all chemical reactions in a cell. It includes breaking down complex molecules for energy (catabolism) and building new molecules using that energy (anabolism).

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Why is Metabolism Important?

Understanding metabolism is crucial because it helps us find ways to control bacterial growth by targeting the specific metabolic pathways that are essential for their survival.

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What are the 3 Main Metabolic Pathways?

Bacteria use three main metabolic pathways to get energy: aerobic respiration (with oxygen), anaerobic respiration (without oxygen), and fermentation.

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Aerobic Respiration

Energy production using oxygen as the final electron acceptor, converting it to water (H2O). It involves three coupled pathways: glycolysis, the Krebs cycle, and the electron transport chain.

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Anaerobic Respiration

Energy production without oxygen. Uses alternative electron acceptors, such as nitrates, sulfates, or carbonates. Less ATP is produced because only parts of the TCA cycle and electron transport chain operate.

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Fermentation

Incomplete glucose breakdown without oxygen, using organic compounds as electron acceptors. It yields a small amount of ATP and produces byproducts like ethanol (in yeast) or acids (in bacteria).

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Final Electron Acceptor

The molecule that receives electrons at the end of an electron transport chain, enabling the release of energy to produce ATP. In aerobic respiration, this is oxygen; in anaerobic respiration, other molecules like nitrates or sulfates serve this role.

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What is the main difference between anaerobic respiration and fermentation?

Anaerobic respiration uses an electron transport chain with inorganic molecules as the final electron acceptor, yielding more ATP than fermentation. Fermentation does not use an electron transport chain and uses organic molecules as final electron acceptors, producing much less ATP, but generating byproducts like alcohol or acids.

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Peptidoglycan

A complex molecule found in bacterial cell walls, composed of sugars (N-acetylglucosamine and N-acetylmuramic acid) and amino acids. It gives strength and rigidity to the bacterial cell.

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Gram-positive bacteria

Bacteria with a thick peptidoglycan layer that stains purple with Gram staining. This layer makes them resistant to certain antibiotics.

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Gram-negative bacteria

Bacteria with a thin peptidoglycan layer and an outer membrane containing lipopolysaccharides. They stain pink with Gram staining.

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Lipopolysaccharide (LPS)

A complex molecule found in the outer membrane of Gram-negative bacteria. It plays a role in the bacteria's structure and can trigger a strong immune response in the host.

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Teichoic acids

Polymers found in the cell wall of Gram-positive bacteria. They help with the absorption of magnesium by the cell.

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Capsule (bacterial)

A protective layer of polysaccharides that surrounds some bacteria. It helps the bacteria evade the host's immune system and is often associated with virulence.

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Bacterial nutrition

The process by which bacteria acquire nutrients from their environment to fuel their cellular activities.

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Virulence

The degree to which a pathogen is able to cause disease. It can be increased by factors like a capsule that protects the bacteria from the immune system.

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Taxonomy

A system for organizing, classifying, and naming living organisms. It focuses on classification and nomenclature.

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Three-Domain System

The modern taxonomic system that classifies organisms into three domains: Archaea, Bacteria, and Eukarya. It was developed by Dr. Carl Woese in 1990.

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Species

The basic unit of taxonomy, representing a specific recognized type of organism. For sexually reproducing organisms, a fundamental definition of 'species' is reproductive compatibility.

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Binomial Nomenclature

A system for scientifically naming organisms using two parts: the genus name and the species name. It is written in italics or underlined.

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Strain

A subgroup within a species with one or more characteristics that distinguish it from other subgroups. They are not genetically identical.

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What are the major branches of Microbiology?

Microbiology includes Bacteriology (study of bacteria), Virology (study of viruses), Mycology (study of fungi), and Parasitology (study of parasites).

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What is Medical Microbiology?

A specialized area of microbiology focused on understanding microorganisms that cause diseases in humans.

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What separates Medical Microbiology from General Microbiology?

Medical Microbiology specifically deals with identifying and controlling microbes that cause diseases in humans.

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Why is understanding Microbial Growth important?

Knowing how microbes grow and reproduce helps us develop strategies to control their growth and prevent infections.

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What are Antimicrobial Agents?

Substances that kill or inhibit the growth of microorganisms.

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How do Antimicrobial Agents work?

Antimicrobial agents target essential processes in microbes, like cell wall synthesis, protein synthesis, or DNA replication.

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What is the importance of Host-Pathogen Relationships?

Understanding this interaction explains how microbes cause disease and helps us develop treatments and preventive strategies.

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Bacteria: Simple Cell Structure

Bacteria are single-celled organisms with a simpler structure than other organisms. They lack a nucleus and membrane-bound organelles like mitochondria.

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Bergey's Manual: Identifying Bacteria

Bergey's Manual of Determinative Bacteriology helps scientists identify bacteria based on their visible characteristics, like shape, staining properties, and how they break down food.

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Phylogenetic Classification

Bergey's Manual of Systematic Bacteriology uses DNA information, specifically rRNA sequences, to understand how bacteria are related to each other.

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What are Mycobank and Index Fungorum?

Mycobank and Index Fungorum are databases that help scientists classify and identify different types of fungi.

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Naming Bacteria: Binomial System

Bacteria are named using a two-part system called binomial nomenclature. The first part is the genus name (always capitalized) and the second is the species name (lowercase).

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Virus Replication

Viruses are genetic elements that can replicate only within living cells (bacteria, animals, or plants). They cannot reproduce independently due to their lack of cellular machinery.

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Obligate Intracellular Parasites

Viruses are called obligate intracellular parasites because they cannot carry out metabolic functions on their own and require a host cell's resources for replication.

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Virion

The extracellular form of a virus, also known as a virus particle, is called a virion. It's metabolically inactive and doesn't perform any life processes.

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Enveloped Virus

A virus with a lipid envelope surrounding its protein coat (capsid).

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Naked Virus

A virus lacking a lipid envelope, possessing only a protein coat (capsid).

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Nucleocapsid

The core structure of a virus, consisting of its nucleic acid (DNA or RNA) enclosed within its protein coat (capsid).

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Virus Classification: Animal vs. Plant

Viruses are classified based on the type of organism they infect, such as animal viruses or plant viruses.

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Virus Classification: DNA vs. RNA

Viruses are further classified based on their genetic material: either DNA or RNA.

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Pasteurization

A heat treatment process that kills harmful microbes in liquids like milk and beer without significantly altering their taste.

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Antiseptic Surgery

Surgical techniques that use chemicals to kill microbes on instruments and around wounds to prevent infections.

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Germ Theory

The idea that microorganisms are responsible for causing diseases.

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Koch's Postulates

A set of four criteria used to prove that a specific microbe causes a specific disease.

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What is the first postulate?

The microorganism must be found in abundance in all organisms suffering from the disease, but should not be found in healthy organisms.

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What is the second postulate?

The microorganism must be isolated from a diseased organism and grown in pure culture.

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What is the third postulate?

The cultured microorganism should cause disease when introduced into a healthy organism.

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What is the fourth postulate?

The microorganism must be re-isolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent.

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What are the two main types of metabolic reactions?

The two main types of metabolic reactions are anabolism (biosynthesis) and catabolism (degradation). Anabolism builds complex molecules using energy, while catabolism breaks down complex molecules to release energy.

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Energy source

This refers to the source of energy that a bacterium uses to perform its metabolic processes. The main types are: "chemo" (from chemical compounds) and "photo" (from light).

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Carbon source

This refers to the source of carbon that a bacterium uses to build its cellular components. The main types are: "organo" (from organic compounds) and "litho" (from inorganic compounds).

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Electron acceptor

This is the molecule that receives electrons during energy production in a metabolic pathway. It's essential for the release of energy from food molecules. The main types are: "oxygen" (aerobic respiration) and "non-oxygen" (anaerobic respiration and fermentation).

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What is a prokaryote?

A cell that lacks a membrane-bound nucleus and other organelles. Examples include bacteria and archaea.

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What is a bacterial chromosome?

A single, circular molecule of DNA that carries most of a bacterial cell's genetic information.

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What are plasmids?

Small, circular pieces of DNA found in some bacteria that can carry additional genes. These genes can benefit the bacteria in certain situations, like antibiotic resistance.

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Compare bacteria and eukaryotic cells.

Bacteria are much smaller than eukaryotic cells and lack organelles. Eukaryotic cells have a nucleus and more complex structures.

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Give examples of fungal diseases.

Fungal diseases can range from mild skin rashes (like athlete's foot) to serious lung infections (like aspergillosis) and even life-threatening conditions (like fungal meningitis).

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What makes a parasite unique?

A parasite is an organism that lives in or on another organism (the host) and benefits by taking nutrients from the host, often harming it.

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Name the three main classes of human parasites

The three main classes of human parasites are protozoa, helminths, and ectoparasites.

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How do protozoa and helminths differ?

Protozoa are single-celled organisms that can multiply inside humans, while helminths are multicellular, large organisms that can't multiply inside humans.

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Bacterial Morphology

The shape of a bacterial cell, which can be round (coccus), rod-shaped (bacillus), curved (vibrio), spiral (spirillum), or corkscrew-shaped (spirochete).

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Streptococcus

A type of bacteria that forms chains of round cells. It can cause infections like strep throat.

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Staphylococcus

A type of bacteria that forms clusters of round cells. It can cause skin infections and food poisoning.

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Escherichia coli (E. coli)

A type of rod-shaped bacteria that is common in the intestines. Most strains are harmless, but some can cause food poisoning.

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Bacillus anthracis

A type of rod-shaped bacteria that causes anthrax, a serious disease that can affect animals and humans.

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Vibrio cholerae

A type of curved bacteria that causes cholera, a severe diarrheal disease.

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Treponema pallidum

A type of corkscrew-shaped bacterium that causes syphilis, a sexually transmitted disease.

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Flagella

Long, whip-like structures that help bacteria move. They are made of a protein called flagellin.

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Periplasmic Space

In Gram-negative bacteria, the periplasmic space is a region between the cell wall and cell membrane. It's like a 'pre-digestion' zone where larger molecules break down before entering the cell.

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Why is Medical Microbiology important?

Medical Microbiology is important because it helps us understand how microbes cause diseases, develop vaccines and antibiotics, and create effective sanitation practices to prevent infections.

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What is Koch's Postulates?

Koch's Postulates are a set of four criteria used to prove that a specific microbe causes a specific disease. They provide a rigorous framework for establishing a cause-and-effect relationship between a microbe and a disease.

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What is a virion?

A virion is a fully formed virus particle found outside of a host cell. It's metabolically inactive, meaning it doesn't perform any life processes on its own.

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What are the main types of metabolic reactions?

The main types of metabolic reactions are anabolism (biosynthesis) and catabolism (degradation). Anabolism uses energy to build complex molecules, while catabolism breaks down complex molecules to release energy.

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Study Notes

Introduction to Microbiology

  • This course introduces the study of microorganisms, including their physiology, ecology, and taxonomy.
  • It emphasizes the impact of microorganisms on human health and the consequences of microbial infection.

Contact Details

Course Sections

  • Microbiology: Basic principles of medical microbiology, general principles of laboratory diagnosis, bacteriology, virology, mycology, parasitology

Lectures

  • Introduction to Medical Microbiology
  • Microbial growth and control
  • Antimicrobial agents
  • Host-pathogen relationships in microbial infections
  • Diagnostic microbiology
  • Coccus (Staphylococci, Streptococci, Enterococci, Neisseria)
  • Gram-positive Bacilli (Bacillus, Corynebacterium, Listeria, Clostridium)
  • Gram-negative Bacilli (Enterobacteriaceae, Vibrio, Pseudomonas, Campylobacter, Helicobacter)
  • Cocobacilli and Mycobacteria (Haemophilus, Bordetella, Brucella, Francisella, Legionella, Mycobacterium, Nocardia)
  • Spirochaetes and Mycoplasmas (Treponema, Borrelia, Leptospira, Mycoplasma, Ureaplasma)
  • Intracellular bacteria (Rickettsia, Orientia, Ehrlichia, Anaplasma, Coxiella, Chlamydiaceae)
  • Viruses and other subcellular agents
  • Clinical Virology (DNA and RNA Virus)
  • Frequent infections by fungal agents
  • Frequent infections by parasitic agents

References

  • MEDICAL MICROBIOLOGY 7TH EDITION, Patrick R Murray; Ken S Rosenthal; Michael A Pfaller
  • PRACTICAL MEDICAL MICROBIOLOGY FOR CLINICIANS, Frank E. Berkowitz, Robert C. Jerris

What is Microbiology?

  • Microbiology studies small (micro) living organisms and diverse topics like their physiology, ecology, and taxonomy.
  • The subject focuses on the impact of microorganisms on human health and the consequences of microbial infection.

History of Microbiology

  • 1590: Zacharias Janssen built the first simple microscope.
  • 1665: Robert Hooke observed cells through a microscope.
  • 1677: Anton van Leeuwenhoek, the "Father of Microbiology," first observed microorganisms.
  • 1796: Edward Jenner pioneered vaccination.
  • 1846: Ignaz Semmelweis implemented hand washing to reduce maternal mortality.
  • 1864: Louis Pasteur invented Pasteurization.
  • 1867: Sir Joseph Lister applied Pasteur's principles for antiseptic surgery.
  • 1876: Robert Koch provided the first proof of germ theory.
  • 1892: Dmitri Ivanovski's work laid the foundation for virology.
  • 1910: Paul Ehrlich invented chemotherapy.
  • 1928: Sir Alexander Fleming discovered penicillin
  • 1931-38: Max Knoll and Ernst Ruska developed the prototype of the electron microscope

Role of Microorganisms

  • Microbes play various roles in basic science, agriculture, food microbiology, public health, and epidemiology, including shaping evolutionary theory, photosynthesis, decomposition, food production, infectious diseases, and the healthy microbiome.

Bacteria

  • Bacteria are single-celled prokaryotes.
  • Estimated hundreds of thousands of bacterial species, with only about 5500 described in detail.

Bacteria structure

  • Bacterial structure includes intracellular structures such as the chromosome, plasmids, ribosomes and the cell membrane.
  • Extracellular structures include flagella, pili, cell wall, and capsule (not always).

Fungi

  • Fungi are eukaryotes found in soil, plants, and humans.
  • Many are common, but some fungal diseases (like meningitis and bloodstream infections) are less frequent but deadly.

Parasites

  • A parasite lives on or in a host, gaining nourishment at the host's expense.
  • Three main types causing human disease: protozoa, helminths, and ectoparasites.

Types of Parasites

  • Protozoa: microscopic, single-celled organisms.
  • Helminths: large, multicellular organisms.
  • Ectoparasites: organisms like ticks, fleas, lice, and mites.

Virus

  • Viruses are genetic elements replicating inside host cells (bacteria, animals, plants).
  • Viruses are characterized by being obligate intracellular parasites.
  • Viruses have an extracellular state called a virion and are metabolically inactive in this state.

Origin of Viruses

  • The origin of viruses is uncertain, but two hypotheses suggest possibilities like:
  • Fugitive nucleic acid pieces breaking off from larger entities, and
  • Viruses may have once lived independently and then lost their genes for autonomous existence.

Virion Structure

  • DNA or RNA viruses, with either protein/lipidic coat or neither.
  • The structure can vary in size, shape, and chemical composition.
  • A capsid contains nucleic acid (DNA or RNA). Complete complex of nucleic acid and protein is called nucleocapsid, which may be enveloped in a membrane(enveloped virus) or not(naked virus)

Virus Classification

  • Classified based on species infected, presence/absence of lipid envelope, symmetry of nucleocapsid, type of nucleic acid, number of nucleic acid strands and structure and polarity of the viral genome

Taxonomy

  • Taxonomy is a system for organizing, classifying, and naming living organisms.
  • Modern taxonomy uses a three-domain system (archaea, bacteria, eucarya).

Specific Examples of Species

  • Examples include Klebsiella pneumoniae, Francisella tularensis, Streptococcus pyogenes, Trypanosoma cruzi, Escherichia coli, Bacillus anthracis.

How to name Bacteria

  • Bergey's Manual of Determinative Bacteriology: uses phenotypic (morphological and biochemical) characteristics.
  • Bergey's Manual of Systematic Bacteriology: uses genetic information (rRNA sequencing).

How to name Fungi, Parasites, and Viruses

Bacterial Classification, Morphology, Structure, and Metabolism

  • Focuses on the further study of bacteria.
  • Covers classification, morphology details, and bacterial structural components (cellular and extracellular components).
  • Includes discussion of the function and role of the components and differences between gram-positive and gram-negative bacteria
  • Covers various structures such as cell wall, pili, flagella, and capsule
  • Examines bacterial metabolic characteristics.
  • Microbial metabolism is classified according to energy and carbon source, and electron acceptor.
  • Three categories of bacterial energy production: aerobic respiration, anaerobic respiration, fermentation
  • Important Concepts
  • Gram-positive and Gram-negative bacteria differ in cell wall structure.
  • Lipopolysaccharide is found in the outer membrane of Gram-negative bacteria.
  • Various types of microbial nutrition and metabolic diversity are included
  • The information for bacterial classification in phyla, classes, orders, families, genera, and species, plus subtypes.

Microbiome

  • The human microbiome comprises microbes in the human body.
  • Includes the roles and functions of the core microbiome, and how the host and microbiome interactions are vital for human health

2020 Coronavirus Pandemic

  • First observed in 2019, a novel coronavirus emerged from animals.
  • Causing an outbreak in Wuhan, China, it progressed into an epidemic, then a pandemic.

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