Bacterial Lactose Metabolism and the lac Operon

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Questions and Answers

What is the main function of inducible operons?

  • Fine-tune transcrip=on to match the metabolic needs of the cell
  • Increase glucose sensitivity in bacteria
  • Activate transcrip=on of the operon based on the substrate (correct)
  • Repress metabolic pathways

What is the primary role of operons in bacterial genomes?

  • Control protein translation
  • Govern DNA replication
  • Modulate cellular respiration
  • Coordinate transcriptional regulation of genes in the same pathway (correct)

Which operon in E.coli is responsible for producing polypeptides needed for lactose utilization?

  • Mal operon
  • Trp operon
  • Glc operon
  • Lac operon (correct)

What describes the regulatory region shared by genes in an operon?

<p>Operator (D)</p> Signup and view all the answers

How do inducible operons differ from repressible operons?

<p>Operate through activation by a substrate (C)</p> Signup and view all the answers

What is the preferred energy source for E.coli?

<p>Glucose (B)</p> Signup and view all the answers

What is the most common structural feature of regulatory DNA-binding proteins in bacteria?

<p>Helix-turn-helix motif (A)</p> Signup and view all the answers

How do the polypeptides of a regulatory homodimer interact with DNA sequences?

<p>Each polypeptide interacts with one repeat unit. (D)</p> Signup and view all the answers

What is the significance of the 'turn' in the helix-turn-helix motif of regulatory DNA-binding proteins?

<p>It separates a recognition helix and a stabilizing helix. (A)</p> Signup and view all the answers

What was the outcome of the mutational analysis of the lac operon mutants?

<p>Continuous transcription of lacZ and lacY genes despite lactose availability (C)</p> Signup and view all the answers

Which of the following best describes the cons%tu%ve mutants in the lac operon?

<p>They prevent the transcription of lacZ and lacY genes regardless of lactose availability. (B)</p> Signup and view all the answers

What is the role of complementation analysis in studying lac operon mutants?

<p>To assign mutations into different complementation groups (D)</p> Signup and view all the answers

What compound is the real inducer of the lac operon?

<p>Allolactose (C)</p> Signup and view all the answers

What is the function of the lac operon?

<p>Produce enzymes for lactose metabolism (C)</p> Signup and view all the answers

Which enzyme is encoded by the lacY gene within the lac operon?

<p>Lactose permease (A)</p> Signup and view all the answers

In the lac operon, what is the function of the lac repressor protein?

<p>Prevents transcription in the absence of glucose (C)</p> Signup and view all the answers

What happens when lactose is available but glucose is also present in the cell?

<p>lac repressor binds to the operator (D)</p> Signup and view all the answers

In a repressible operon, what causes transcription of the operon to be blocked?

<p>Presence of end product (D)</p> Signup and view all the answers

When tryptophan is absent in the cell, what happens in the trp operon?

<p>Transcription of trp operon is induced (A)</p> Signup and view all the answers

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