Bacterial Lactose Metabolism and the lac Operon

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What is the main function of inducible operons?

Fine-tune transcrip=on to match the metabolic needs of the cell

Increase glucose sensitivity in bacteria

Activate transcrip=on of the operon based on the substrate (correct)

Repress metabolic pathways

What is the primary role of operons in bacterial genomes?

Control protein translation

Govern DNA replication

Modulate cellular respiration

Coordinate transcriptional regulation of genes in the same pathway (correct)

Which operon in E.coli is responsible for producing polypeptides needed for lactose utilization?

Mal operon

Trp operon

Glc operon

Lac operon (correct)

What describes the regulatory region shared by genes in an operon?

Operator Signup and view all the answers

How do inducible operons differ from repressible operons?

Operate through activation by a substrate Signup and view all the answers

What is the preferred energy source for E.coli?

Glucose Signup and view all the answers

What is the most common structural feature of regulatory DNA-binding proteins in bacteria?

Helix-turn-helix motif Signup and view all the answers

How do the polypeptides of a regulatory homodimer interact with DNA sequences?

Each polypeptide interacts with one repeat unit. Signup and view all the answers

What is the significance of the 'turn' in the helix-turn-helix motif of regulatory DNA-binding proteins?

It separates a recognition helix and a stabilizing helix. Signup and view all the answers

What was the outcome of the mutational analysis of the lac operon mutants?

Continuous transcription of lacZ and lacY genes despite lactose availability Signup and view all the answers

Which of the following best describes the cons%tu%ve mutants in the lac operon?

They prevent the transcription of lacZ and lacY genes regardless of lactose availability. Signup and view all the answers

What is the role of complementation analysis in studying lac operon mutants?

To assign mutations into different complementation groups Signup and view all the answers

What compound is the real inducer of the lac operon?

Allolactose Signup and view all the answers

What is the function of the lac operon?

Produce enzymes for lactose metabolism Signup and view all the answers

Which enzyme is encoded by the lacY gene within the lac operon?

Lactose permease Signup and view all the answers

In the lac operon, what is the function of the lac repressor protein?

Prevents transcription in the absence of glucose Signup and view all the answers

Bacterial Lactose Metabolism and the lac Operon

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Questions and Answers

What is the main function of inducible operons?

What is the primary role of operons in bacterial genomes?

Which operon in E.coli is responsible for producing polypeptides needed for lactose utilization?

What describes the regulatory region shared by genes in an operon?

How do inducible operons differ from repressible operons?

What is the preferred energy source for E.coli?

What is the most common structural feature of regulatory DNA-binding proteins in bacteria?

How do the polypeptides of a regulatory homodimer interact with DNA sequences?

What is the significance of the 'turn' in the helix-turn-helix motif of regulatory DNA-binding proteins?

What was the outcome of the mutational analysis of the lac operon mutants?

Which of the following best describes the cons%tu%ve mutants in the lac operon?

What is the role of complementation analysis in studying lac operon mutants?

What compound is the real inducer of the lac operon?

What is the function of the lac operon?

Which enzyme is encoded by the lacY gene within the lac operon?

In the lac operon, what is the function of the lac repressor protein?

What happens when lactose is available but glucose is also present in the cell?

In a repressible operon, what causes transcription of the operon to be blocked?

When tryptophan is absent in the cell, what happens in the trp operon?

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