Bacterial Identification Quiz

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Questions and Answers

What type of hemolysis does E. faecalis exhibit on BAP?

  • Gamma hemolytic
  • Alpha hemolysis
  • Beta hemolysis (correct)
  • No hemolysis

Which test shows a positive result for S. agalactiae?

  • Hippurate hydrolysis
  • 6.5% NaCl (correct)
  • Catalase
  • PYR

What is the colony shape of S. agalactiae on BAP?

  • Small / convex (correct)
  • Large / flat
  • Medium / irregular
  • Rough / irregular

Which organism shows positive results in the PYR test?

<p>S. agalactiae (C)</p> Signup and view all the answers

What is a distinguishing characteristic of Micrococcus luteus compared to E. faecalis and S. agalactiae based on the tests indicated?

<p>It is catalase positive (B)</p> Signup and view all the answers

Which bacteria is identified as being positive for coagulase testing?

<p>Staphylococcus aureus (A)</p> Signup and view all the answers

What does a gamma hemolysis result indicate about a bacterial organism?

<p>No lysis of red blood cells (A)</p> Signup and view all the answers

Which organism is identifiable by its yellow or white colonies on culture media?

<p>M. luteus (D)</p> Signup and view all the answers

In the context of bacterial identification, what does a positive catalase test signify?

<p>Ability to break down hydrogen peroxide (B)</p> Signup and view all the answers

What biochemical reaction is indicated by a positive optochin sensitivity test?

<p>Streptococcus pneumoniae identification (B)</p> Signup and view all the answers

What type of bacteria is organism #8 based on its Gram stain result?

<p>Gram-positive (D)</p> Signup and view all the answers

Which growth characteristic is observed for organism #9 on Blood Agar Plate (BAP)?

<p>Small, circular colonies (A)</p> Signup and view all the answers

What is the arrangement of organism #9 based on the provided observations?

<p>Singles or diplococci (D)</p> Signup and view all the answers

Which of the following characteristics was not provided for organism #8?

<p>6.5% NaCl tolerance (D)</p> Signup and view all the answers

What type of hemolysis is observed for organism #9 on the Blood Enrichment Agar (BEA)?

<p>Alpha-hemolysis (A)</p> Signup and view all the answers

What reaction did organism #8 display in the catalase test?

<p>Negative (B)</p> Signup and view all the answers

Among the following features of organism #9, which one indicates a potential identification feature?

<p>Alpha-hemolysis on BEA (A)</p> Signup and view all the answers

What was the result of the hippurate test for organism #9?

<p>Negative (D)</p> Signup and view all the answers

Flashcards

Gram-positive Cocci

Bacteria that appear purple after Gram staining, are spherical, and occur in clusters.

Hemolysis test Result

Tests for the ability of a bacterial organism to break down red blood cells.

Staphylococcus Aureus Identification

A Gram-positive coccus characterized by catalase-positive and coagulase-positive reactions, and often forming colonies that appear yellow/white/pink/milky.

Optochin Sensitivity

A test measuring how sensitive a bacterium is to Optochin; the result is typically categorized as susceptible (S) or resistant (R).

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Bacterial Identification

Process of determining the species of bacteria based on their observed characteristics (like Gram stain, shape, growth, and biochemical reactions).

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Gram Stain

A staining technique used to classify bacteria based on their cell wall structure. Bacteria with a thick peptidoglycan layer retain the crystal violet dye and appear purple after Gram staining, while those with thinner layers lose the dye and appear pink after counterstaining with safranin.

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Hemolysis

The ability of a bacterial organism to break down red blood cells. Different patterns of hemolysis can be observed on blood agar plates, indicating different types of hemolytic activity.

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Catalase Test

A test used to identify bacteria that produce the enzyme catalase. The presence of catalase can be detected by bubbling when hydrogen peroxide is added to a bacterial culture.

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Colony Morphology

The physical characteristics of a bacterial colony on an agar plate, such as shape, size, color, and texture.

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What does 'Beta Hemolysis' mean on a blood agar plate?

Beta hemolysis refers to the complete lysis (breakdown) of red blood cells around a bacterial colony on a blood agar plate, resulting in a clear zone of hemolysis.

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Cocci

Spherical or round-shaped bacteria.

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Diplococci

Pairs of spherical bacteria.

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Alpha-Hemolysis

A type of hemolysis where red blood cells around bacterial colonies are partially broken down, resulting in a greenish discoloration.

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Beta-Hemolysis

A type of hemolysis where red blood cells around bacterial colonies are completely broken down, resulting in a clear zone around the colonies.

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Lancefield Grouping

A system for classifying streptococcal bacteria based on their cell wall antigens.

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