Podcast
Questions and Answers
What occurs during the lysogenic cycle of a bacteriophage?
What occurs during the lysogenic cycle of a bacteriophage?
- The phage DNA integrates at a specific site on bacterial DNA. (correct)
- Only RNA viruses can undergo this cycle.
- The bacterial cell is completely lysed immediately.
- The phage DNA is replicated without integration.
Which mechanism describes the transfer of DNA between two bacterial cells through direct contact?
Which mechanism describes the transfer of DNA between two bacterial cells through direct contact?
- Transduction
- Transformation
- Conjugation (correct)
- Binary Fission
What is formed when the donor cell's F plasmid is transferred to the recipient cell during conjugation?
What is formed when the donor cell's F plasmid is transferred to the recipient cell during conjugation?
- An F- cell
- A transducing phage
- An Hfr cell
- An F+ cell (correct)
What is the primary role of the F pili during bacterial conjugation?
What is the primary role of the F pili during bacterial conjugation?
In specialized transduction, which part of the bacterial chromosome is primarily involved in the genetic transfer?
In specialized transduction, which part of the bacterial chromosome is primarily involved in the genetic transfer?
What is the primary form of bacterial genetic material?
What is the primary form of bacterial genetic material?
Which type of extra chromosomal DNA can replicate independently within bacteria?
Which type of extra chromosomal DNA can replicate independently within bacteria?
Which component of plasmids is responsible for antibiotic resistance?
Which component of plasmids is responsible for antibiotic resistance?
What is transposition in bacterial genetics?
What is transposition in bacterial genetics?
What is a distinguishing feature of transposons compared to plasmids?
What is a distinguishing feature of transposons compared to plasmids?
How many copies of an F-plasmid are typically present in a bacterial cell?
How many copies of an F-plasmid are typically present in a bacterial cell?
What is the function of tra genes found in plasmids?
What is the function of tra genes found in plasmids?
What makes IS transposable elements distinctive?
What makes IS transposable elements distinctive?
What process allows the spread of antibiotic resistance among bacteria?
What process allows the spread of antibiotic resistance among bacteria?
Which organism is known for exhibiting antigenic variation through programmed rearrangements?
Which organism is known for exhibiting antigenic variation through programmed rearrangements?
How can a bacterial cell become competent to uptake naked DNA?
How can a bacterial cell become competent to uptake naked DNA?
What role do bacteriophages play in DNA transfer between bacteria?
What role do bacteriophages play in DNA transfer between bacteria?
Which method involves direct uptake of naked DNA by a recipient cell?
Which method involves direct uptake of naked DNA by a recipient cell?
What is the result of a chromosome fragment being encapsulated in a phage coat?
What is the result of a chromosome fragment being encapsulated in a phage coat?
What happens to bacterial cells during the lysogenic phase after bacteriophage integration?
What happens to bacterial cells during the lysogenic phase after bacteriophage integration?
What mechanism involves the transfer of DNA between bacteria via a virus?
What mechanism involves the transfer of DNA between bacteria via a virus?
Which statement accurately describes transformation in bacteria?
Which statement accurately describes transformation in bacteria?
What is a significant trait of generalized transduction?
What is a significant trait of generalized transduction?
Which of the following is NOT a role of plasmids in bacteria?
Which of the following is NOT a role of plasmids in bacteria?
Which of the following best defines a non-conjugative plasmid?
Which of the following best defines a non-conjugative plasmid?
How do specialized transduction and generalized transduction differ?
How do specialized transduction and generalized transduction differ?
Which term is used to describe the process where bacteria acquire DNA from their environment?
Which term is used to describe the process where bacteria acquire DNA from their environment?
What can be inferred about the spread of antibiotic resistance genes in Proteus species?
What can be inferred about the spread of antibiotic resistance genes in Proteus species?
What is the significance of the integrated F factor in bacterial cells?
What is the significance of the integrated F factor in bacterial cells?
During specialized transduction, what happens to the bacterial chromosome?
During specialized transduction, what happens to the bacterial chromosome?
Which of the following best describes the role of F pili in bacterial conjugation?
Which of the following best describes the role of F pili in bacterial conjugation?
What type of bacterial cells predominantly experience plasmid transfer through conjugation?
What type of bacterial cells predominantly experience plasmid transfer through conjugation?
In the context of DNA transfer, what occurs when a donor cell with an F plasmid connects to a recipient cell?
In the context of DNA transfer, what occurs when a donor cell with an F plasmid connects to a recipient cell?
How does programmed rearrangement contribute to bacterial survival?
How does programmed rearrangement contribute to bacterial survival?
What role does calcium chloride play in transforming bacterial cells?
What role does calcium chloride play in transforming bacterial cells?
What happens during transduction mediated by bacteriophages?
What happens during transduction mediated by bacteriophages?
What characterizes the lytic cycle of a bacteriophage?
What characterizes the lytic cycle of a bacteriophage?
How do competent cells allow for the uptake of naked DNA?
How do competent cells allow for the uptake of naked DNA?
What is the role of bacteriophages in horizontal gene transfer?
What is the role of bacteriophages in horizontal gene transfer?
What is the mechanism by which a bacterium can acquire DNA from the environment?
What is the mechanism by which a bacterium can acquire DNA from the environment?
What is the significance of antigenic variation in bacteria?
What is the significance of antigenic variation in bacteria?
What mechanism allowed Proteus species to acquire antibiotic resistance genes similar to those in Pseudomonas and Escherichia?
What mechanism allowed Proteus species to acquire antibiotic resistance genes similar to those in Pseudomonas and Escherichia?
In what way do generalized transduction and specialized transduction differ?
In what way do generalized transduction and specialized transduction differ?
Which of the following statements accurately describes transformation in bacteria?
Which of the following statements accurately describes transformation in bacteria?
Which characteristic differentiates transposons from plasmids in terms of replication?
Which characteristic differentiates transposons from plasmids in terms of replication?
Which component plays a critical role in bacterial conjugation?
Which component plays a critical role in bacterial conjugation?
What is a significant function of the tra genes found in certain plasmids?
What is a significant function of the tra genes found in certain plasmids?
What is the primary source of genetic material transferred during transduction?
What is the primary source of genetic material transferred during transduction?
Which statement regarding naked DNA in transformation is true?
Which statement regarding naked DNA in transformation is true?
Which type of plasmid is primarily responsible for carrying genes related to antibiotic resistance?
Which type of plasmid is primarily responsible for carrying genes related to antibiotic resistance?
What essential property do transposons possess that enables them to integrate into the genome?
What essential property do transposons possess that enables them to integrate into the genome?
Why might a bacterium prefer transformation as a means of genetic exchange?
Why might a bacterium prefer transformation as a means of genetic exchange?
What describes the role of bacteriophages in generalized transduction?
What describes the role of bacteriophages in generalized transduction?
What best describes the typical structure of plasmids found in bacteria?
What best describes the typical structure of plasmids found in bacteria?
Which characteristic of transposons is associated with their classification as 'jumping genes'?
Which characteristic of transposons is associated with their classification as 'jumping genes'?
Which of the following statements accurately depicts the role of plasmids in bacterial genetics?
Which of the following statements accurately depicts the role of plasmids in bacterial genetics?
What is the primary function of the 'F-plasmid' in bacteria?
What is the primary function of the 'F-plasmid' in bacteria?
Flashcards
Bacterial Genome
Bacterial Genome
The complete set of genetic information in a bacterium, typically a single circular DNA molecule.
Nucleoid
Nucleoid
The region within a bacterial cell where the circular DNA molecule is located.
Plasmid
Plasmid
Small, circular DNA molecule that exists independently of the bacterial chromosome.
Transposon
Transposon
A piece of DNA that can move from one location to another within the genome.
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Transposition
Transposition
The process of a transposon being excised and re-integrated into the DNA molecule.
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IS Element
IS Element
The simplest type of transposon, encoding only the proteins needed for its own transposition.
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Composite Transposon
Composite Transposon
A transposon that contains two IS elements flanking a central piece of DNA that might carry genes like antibiotic resistance.
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Tn3 Transposon
Tn3 Transposon
A type of transposon that doesn't have IS elements but still encodes proteins for transposition and carries genes for antibiotic resistance or virulence.
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Antibiotic Resistance Spread
Antibiotic Resistance Spread
Transfer of a plasmid containing antibiotic resistance genes from one bacterium to another via conjugation.
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Antigenic Variation
Antigenic Variation
Changes in surface proteins of a pathogen, like Neisseria gonorrhoeae, through gene movement, allowing it to evade the host's immune system.
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Transformation
Transformation
The process where a bacterium directly takes up naked DNA from its environment.
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Artificial Transformation
Artificial Transformation
Induced uptake of DNA into a cell in a laboratory setting, often using calcium chloride and heat shock.
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Electroporation
Electroporation
A method to introduce DNA into cells by applying an electric field, creating temporary pores in the membrane.
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Transduction
Transduction
Transfer of DNA from a donor cell to a recipient cell mediated by a bacteriophage.
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Lytic Cycle
Lytic Cycle
A viral replication cycle where a bacteriophage infects a cell, replicates, and lyses the cell, releasing new phages.
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Generalized Transduction
Generalized Transduction
A type of transduction where any piece of bacterial DNA can be packaged inside a phage and transferred to another bacteria.
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Lysogenic Cycle
Lysogenic Cycle
A viral replication cycle where the phage DNA integrates into the host bacterial chromosome, becoming a prophage and replicating along with the host DNA. This integration can lead to specialized transduction.
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Specialized Transduction
Specialized Transduction
A type of transduction where only specific bacterial genes near the prophage insertion site are transferred to a recipient cell. This occurs when the phage DNA is excised from the host chromosome, taking a piece of bacterial DNA with it.
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Conjugation
Conjugation
A process of horizontal gene transfer where bacteria exchange genetic material through direct contact. This involves a donor cell with an F plasmid containing the tra gene, which enables the formation of F pili for attachment and DNA transfer.
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F Factor
F Factor
A plasmid that carries genes for the production of F pili, enabling conjugation. It can integrate into the bacterial chromosome, creating an Hfr cell.
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Hfr Cell
Hfr Cell
A bacterial cell with the F factor integrated into its chromosome. This integration leads to high recombination rates and allows the transfer of chromosomal genes during conjugation.
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Horizontal Gene Transfer
Horizontal Gene Transfer
The transfer of genetic material between organisms that are not related by descent, like bacteria sharing genes through conjugation, transformation, or transduction.
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Non-conjugative Plasmid
Non-conjugative Plasmid
A plasmid that cannot initiate conjugation, as it lacks the necessary genes for pilus formation and DNA transfer.
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Functions of a Plasmid
Functions of a Plasmid
Plasmids can carry genes for antibiotic resistance, toxin production, virulence factors, metabolic pathways, and more, providing bacteria with advantages like survival in harsh environments or even causing disease.
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Laboratory-Induced Competence
Laboratory-Induced Competence
Inducing a bacterium to take up foreign DNA. This is done in labs by using methods like adding calcium chloride and heat shock.
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What are plasmids?
What are plasmids?
Plasmids are small, circular DNA molecules that exist independently of the bacterial chromosome. They replicate independently and can carry genes for various functions, like antibiotic resistance.
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How do plasmids replicate?
How do plasmids replicate?
Plasmids have their own origin of replication, meaning they can replicate independently from the bacterial chromosome. This allows them to exist and multiply even when the bacterial chromosome is not replicating.
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What is a transposon?
What is a transposon?
A transposon is a piece of DNA that can move from one location to another within the genome. They are also known as 'jumping genes' and can carry genes like antibiotic resistance.
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What is transposition?
What is transposition?
Transposition is the process by which a transposon moves from one location to another within a genome. It involves the transposon being excised and then reintegrated at a new location.
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What is an IS element?
What is an IS element?
An IS element is the simplest type of transposon. It only encodes the proteins needed for its own transposition, meaning it can only move itself.
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What is a composite transposon?
What is a composite transposon?
A composite transposon is a more complex transposon containing two IS elements flanking a central piece of DNA. This central region often carries genes like antibiotic resistance.
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What is a Tn3 transposon?
What is a Tn3 transposon?
A Tn3 transposon is a type of transposon that doesn't have IS elements but still encodes proteins for transposition and carries genes like antibiotic resistance or virulence factors.
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How do transposons spread antibiotic resistance?
How do transposons spread antibiotic resistance?
Transposons can 'jump' from one location to another, potentially landing on a plasmid. If this plasmid is then transferred to another bacterium through conjugation, antibiotic resistance genes carried by the transposon are also transferred, spreading resistance.
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Bacterial Genetics
- Bacterial genome: single, circular DNA molecule forming the nucleoid
- Some bacteria have extra-chromosomal DNA: plasmids, transposons
- Plasmids are pieces of DNA separate from the chromosome, containing an origin of replication and independently replicating
- Plasmids carry antibiotic resistance (R plasmids), fertility (F plasmids), toxins, enzymes, and bacteriocin
- Plasmids are used as cloning vectors in molecular biology
- Plasmids can exist as various sizes and copy numbers (1-2 copies/cell, 10-15 copies/cell, etc.)
- Plasmids are typically circular and closed-circular (CCC)
- Some linear plasmids are unstable due to exonuclease attack
- Conjugative plasmids have tra genes that mobilize plasmid from one cell to another and are transmissible
- Non-conjugative plasmids can't be mobilized under known conditions
- Natural plasmids exist naturally in bacteria
- Artificial plasmids are modified to be used as vectors
- Transposons: extra chromosomal small pieces of DNA capable of moving themselves from one location to another on the genome
- They are movable elements (jumping genes)
- Transposition is the process by which transposons excise and re-integrate into DNA.
- Transposons often contain an enzyme called transposase
- Some transposons code for drug resistance
- Composite transposons contain two insertion sequences (IS elements) flanking a central region of DNA
- Non-composite transposons do not have IS elements
- Tn10: encodes for tetracycline resistance
- Bacteria possess genes on silent storage locations which can be moved to active sites, resulting in antigenic variations and immune evasion
- Method of Bacterial Gene Transfer, Within the Bacterial Cells, Transposons transfer DNA from one site on bacterial chromosome to another site or to a plasmid, subsequent transfer of the plasmid leads to spread of antibiotic resistance
- Non-composite transposons: do not have IS but contain genes for transposition, antibiotic resistance or virulence
- Plasmids can be important for antibiotic resistance, toxins, or enzymes, among other properties
- Transduction is the transfer of DNA from one bacterium to another by a bacteriophage (virus)
- Bacteriophages can have lytic or lysogenic cycles:
- Lytic cycle: bacteriophage destroys host cells
- Lysogenic cycle: bacteriophage integrates into the host genome
- Conjugation: gene transfer by direct contact between bacteria cells
- Conjugation requires a donor (F+ cell with F plasmid containing tra gene) and a recipient (F- cell)
- F pili form a conjugation bridge enabling plasmid transfer to the recipient (F+ cell)
- An Hfr (High Frequency Recombination) cell occurs when the F plasmid integrates into the host chromosome. Transfer of chromosomal DNA from the donor to the recipient cell during conjugation
- Transformation: direct uptake of naked DNA by a recipient cell
Questions
- Non-conjugative plasmid: a plasmid that cannot trigger the conjugation process
- Transfer of DNA from donor cells to recipient cells by bacteriophage: transduction
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