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Questions and Answers
What is the primary role of the bacterial chromosome?
What is the primary role of the bacterial chromosome?
Which statement accurately describes plasmids in bacteria?
Which statement accurately describes plasmids in bacteria?
What allows plasmids to be transferred between bacteria?
What allows plasmids to be transferred between bacteria?
Which of the following statements about flagella in bacteria is true?
Which of the following statements about flagella in bacteria is true?
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What is a function of plasmids in genetic engineering?
What is a function of plasmids in genetic engineering?
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Which organism is NOT commonly found in the colon?
Which organism is NOT commonly found in the colon?
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What is the first requirement of Koch's postulates?
What is the first requirement of Koch's postulates?
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Which bacterial component is crucial for the identification of microbial pathogens?
Which bacterial component is crucial for the identification of microbial pathogens?
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Which bacterial species is primarily associated with dental plaque?
Which bacterial species is primarily associated with dental plaque?
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Which of the following best describes the structures included in a bacterial cell envelope?
Which of the following best describes the structures included in a bacterial cell envelope?
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Which bacteria is NOT typically found in the urethra?
Which bacteria is NOT typically found in the urethra?
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Which bacterium is known for producing specific antibiotics?
Which bacterium is known for producing specific antibiotics?
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Which of the following organisms can be associated with gum disease?
Which of the following organisms can be associated with gum disease?
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What type of bacteria can tolerate exposure to oxygen despite being anaerobic?
What type of bacteria can tolerate exposure to oxygen despite being anaerobic?
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Which process involves the formation of two identical daughter cells in bacteria?
Which process involves the formation of two identical daughter cells in bacteria?
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Under optimal conditions, how quickly can bacterial populations double?
Under optimal conditions, how quickly can bacterial populations double?
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What is typically used to isolate pure cultures of bacterial strains?
What is typically used to isolate pure cultures of bacterial strains?
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What is the purpose of selective media in bacterial growth?
What is the purpose of selective media in bacterial growth?
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Which type of bacteria requires or prefers reduced oxygen levels?
Which type of bacteria requires or prefers reduced oxygen levels?
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What is the first step in the binary fission of a bacterial cell?
What is the first step in the binary fission of a bacterial cell?
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Which method is effective for growing bacterial cultures in even cell suspensions?
Which method is effective for growing bacterial cultures in even cell suspensions?
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What is the primary feature of the lag phase in bacterial growth?
What is the primary feature of the lag phase in bacterial growth?
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What occurs during the stationary phase of bacterial growth?
What occurs during the stationary phase of bacterial growth?
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How do biofilms benefit bacteria in natural environments?
How do biofilms benefit bacteria in natural environments?
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What is the main component of the bacterial capsule?
What is the main component of the bacterial capsule?
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What phenomenon allows one bacterial species to utilize the metabolic products of another species?
What phenomenon allows one bacterial species to utilize the metabolic products of another species?
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Which of the following bacteria is noted for its beta-hemolysis on blood agar?
Which of the following bacteria is noted for its beta-hemolysis on blood agar?
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Which Gram-positive bacteria characteristic is unique to them?
Which Gram-positive bacteria characteristic is unique to them?
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What role does peptidoglycan play in bacterial cells?
What role does peptidoglycan play in bacterial cells?
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Which component helps prevent bacteria from drying out?
Which component helps prevent bacteria from drying out?
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What type of bacteria are lipopolysaccharides found in?
What type of bacteria are lipopolysaccharides found in?
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What does the cytoplasmic membrane regulate?
What does the cytoplasmic membrane regulate?
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Which of the following is true regarding the nucleoid?
Which of the following is true regarding the nucleoid?
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What does penicillin interfere with in bacterial cells?
What does penicillin interfere with in bacterial cells?
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What type of bacteria are capable of forming endospores?
What type of bacteria are capable of forming endospores?
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Which mechanism allows bacteria to exchange plasmid DNA?
Which mechanism allows bacteria to exchange plasmid DNA?
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Which of the following is NOT a mechanism of generating metabolic energy in bacteria?
Which of the following is NOT a mechanism of generating metabolic energy in bacteria?
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What is the primary role of bacterial ribosomes?
What is the primary role of bacterial ribosomes?
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What distinguishes bacterial ribosomes from eukaryotic ribosomes?
What distinguishes bacterial ribosomes from eukaryotic ribosomes?
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During which condition does sporulation in bacteria typically occur?
During which condition does sporulation in bacteria typically occur?
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Which of the following best defines heterotrophy in bacteria?
Which of the following best defines heterotrophy in bacteria?
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What is a function of pili in bacteria?
What is a function of pili in bacteria?
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What is a characteristic of endospores that contribute to bacterial survival?
What is a characteristic of endospores that contribute to bacterial survival?
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What type of metabolic strategy do Cyanobacteria primarily utilize?
What type of metabolic strategy do Cyanobacteria primarily utilize?
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Flashcards
Corynebacterium (diphtheroids)
Corynebacterium (diphtheroids)
These bacteria are commonly found on the skin, particularly in the areas with hair follicles. They are generally harmless and cause no problems.
Viridans streptococci
Viridans streptococci
These bacteria are part of the normal flora of the mouth and throat, often associated with dental plaque.
Streptococcus mutans
Streptococcus mutans
This species is often associated with dental cavities. Like a tiny party on your teeth.
Prevotella intermedia
Prevotella intermedia
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Porphyromonas gingivalis
Porphyromonas gingivalis
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Streptococcus pyogenes
Streptococcus pyogenes
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Streptococcus pneumoniae
Streptococcus pneumoniae
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Neisseria species
Neisseria species
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Bacterial Chromosome
Bacterial Chromosome
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Plasmids
Plasmids
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Conjugation
Conjugation
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Flagella
Flagella
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Flagellar Movement
Flagellar Movement
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Bacterial Capsule
Bacterial Capsule
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Bacterial Cell Wall
Bacterial Cell Wall
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Peptidoglycan
Peptidoglycan
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Gram-positive Bacteria
Gram-positive Bacteria
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Gram-negative Bacteria
Gram-negative Bacteria
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Cytoplasmic Membrane
Cytoplasmic Membrane
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Cytoplasm
Cytoplasm
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Nucleoid
Nucleoid
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Lag Phase
Lag Phase
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Logarithmic (Log) / Exponential Phase
Logarithmic (Log) / Exponential Phase
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Stationary Phase
Stationary Phase
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Death Phase
Death Phase
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Syntrophy
Syntrophy
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Pili
Pili
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Ribosomes
Ribosomes
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Endospore
Endospore
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Metabolism
Metabolism
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Heterotrophy
Heterotrophy
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Lithotrophy
Lithotrophy
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Phototrophy
Phototrophy
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Autotrophic CO2 fixation
Autotrophic CO2 fixation
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Respiration
Respiration
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Aerotolerant Anaerobe
Aerotolerant Anaerobe
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Microaerophilic
Microaerophilic
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Capnophilic
Capnophilic
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Binary Fission
Binary Fission
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Agar Plates
Agar Plates
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Liquid Growth Media
Liquid Growth Media
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Selective Media
Selective Media
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Bacterial Colonies
Bacterial Colonies
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Study Notes
General Bacteriology - MDP 10208
- Students should be able to define bacteria, describe major structures and functions, list and describe morphological forms, describe bacterial metabolism, growth, and reproduction, and list common diseases and their causative bacterial agents.
Bacteria
- A microscopic unicellular organism in various shapes and sizes.
- Prokaryotic cells.
- Carry out metabolic activities to increase size.
- Reproduce by binary fission (one cell divides into two equal parts).
- Typical size: 0.5-1 µm broad, 0.5-8 µm long.
The Sizes of Microorganisms
- Diagram illustrates the relative sizes of different biological entities, showing the range from atoms and small molecules to viruses, bacteria, organelles, and eukaryotic cells.
- Depicts the size scale relevant to light and electron microscopy.
Bacterial Distribution in the Environment
- Bacteria are prokaryotic organisms.
- Found in habitats where eukaryotes live.
- Found in extreme environments (hottest, coldest, driest).
- May exist in symbiotic/mutualistic associations with eukaryotes.
- Mitochondria and chloroplasts are hypothesized remnants of bacteria.
- Many eukaryotic cells contain endosymbiotic prokaryotes.
- Only some bacteria are disease-causing agents.
Normal Flora
- Bacteria and fungi permanently residing in specific body sites.
- Common locations include skin, oropharynx, colon, and vagina.
- Viruses and parasites are not typically classified as normal flora, although present in asymptomatic individuals.
- Often called commensals—benefit from the host but do no harm.
Medically Important Normal Flora
- Table displays medically important and less important bacterial species present in various body locations (e.g., skin, nose, mouth).
Importance in Medicine
- Provide laboratory models for infectious diseases.
- Allow the production of antibiotics.
- Offer bacterial components for vaccine production.
- Facilitate the identification of microbial pathogens—to target specific treatment.
Koch's Postulate (1884)
- A set of postulates to demonstrate the pathogenicity of a microorganism.
- The organism must be constantly found in all cases of the disease.
- The organism can be isolated and grown outside the host.
- The organism can reproduce the disease in a healthy susceptible host.
- The same organism can be re-isolated from the inoculated host.
Structures of Bacteria - Cell Envelope
- Made up of two or three layers.
- Inner cytoplasmic membrane.
- Cell wall.
- Outer capsule (in some species).
- Capsules are polysaccharide layers surrounding the cell wall. Protect from desiccation, phagocytosis (engulfing by immune cells), and contribute to virulence.
Structures of Bacteria - Cell Wall
- Contains peptidoglycan (protein-polysaccharide).
- Provides cell shape and protects from environmental stresses, including osmotic pressure.
- Critical in bacterial species analysis.
- Components differ between Gram-positive and Gram-negative bacteria, including lipoteichoic acids and lipopolysaccharides, respectively.
Structures of Bacteria - Peptidoglycan
- The glycan portion alternates NAM and NAG sugars.
- Polypeptide cross-links connect the sugars.
- Penicillin interferes with peptidoglycan synthesis.
Structures of Bacteria - Gram-positive Cell Wall
- Diagram illustrating the layered structure with peptidoglycan, teichoic acids, and the cytoplasmic membrane.
Structures of Bacteria - Gram-negative Cell Wall
- Diagram showing the layered structure containing peptidoglycan, outer membrane (with lipopolysaccharides), and the cytoplasmic membrane.
Structures of Bacteria - Cytoplasmic Membrane
- Phospholipid bilayer.
- Controls material flow into and out of the cell.
- Highly organized and dynamic.
Structures of Bacteria - Cytoplasm
- Gel-like substance in the cell.
- Contains water, enzymes, nutrients, wastes, and gases.
- Contains ribosomes, a chromosome, plasmids, and other cellular components.
Structures of Bacteria - Nucleoid
- Region within the cytoplasm that contains the bacterial chromosome.
- Not a membrane-bound nucleus.
- Most cells have a single, circular chromosome.
Structures of Bacteria - Plasmids
- Small circular DNA molecules.
- Replicate independently of the chromosome.
- Provide selective advantages, such as antibiotic resistance.
- Can be transferred to other bacteria during conjugation.
Structures of Bacteria - Flagella
- Protein-based structures for locomotion.
- Propeller-like motion for movement toward nutrients, away from toxic substances, or in the case of photosynthetic bacteria, toward light.
Structures of Bacteria - Pili
- Hair-like protein appendages projecting from the surface.
- Involved in attachment and conjugation.
Structures of Bacteria - Ribosomes
- Composed of proteins and rRNA.
- Essential for protein synthesis.
- Differ structurally from eukaryotic ribosomes.
- Target for some antibiotics.
Structures of Bacteria - Endospores
- Dormant, tough structures produced by some bacteria.
- Allow survival in harsh conditions.
- Found primarily in Gram-positive bacteria (e.g., Bacillus, Clostridium).
Metabolism
- The sum of all chemical processes in living organisms.
- Bacteria exhibit a wide variety of types, with traits used to classify them taxonomically.
- Three major criteria classify bacterial metabolism: carbon source, energy source, and electron donors.
Mechanisms of Generating Metabolic Energy for Bacterial Growth
- Heterotrophy (using organic compounds).
- Lithotrophy (using inorganic compounds).
- Phototrophy (using light).
- Autotrophic CO2 fixation (using CO2 as carbon source).
Respiration
- Complete substrate oxidation using an outside electron acceptor.
- Requires glycolysis, TCA cycle, and electron transport system.
Fermentation
- Partial substrate oxidation using an organic intermediate as electron donor and acceptor.
- No external electron acceptors needed.
- Generates ATP via substrate-level phosphorylation.
Oxygen Requirements of Bacteria
- Aerobic bacteria require oxygen.
- Anaerobic bacteria do not require oxygen and may be harmed by it.
- Facultative anaerobes can survive with or without oxygen.
- Other categories include aerotolerant anaerobes, microaerophiles, and capnophiles.
Atmospheric Growth Requirements
- Different bacteria have different oxygen needs, each defined as a category (strict/obligate aerobes, anaerobes, facultative anaerobes, aerotolerant anaerobes, microaerophiles, etc).
Growth and Reproduction - Binary Fission
- Bacteria increase in size and reproduce through cell division (binary fission).
- Key steps involve DNA duplication, chromosomal replication, cell elongation, and separation to form two identical daughter cells.
Growth and Reproduction - Laboratory Considerations
- Bacteria grow in solid or liquid media.
- Solid media, like agar plates, isolate pure cultures.
- Liquid media may be used for volume and growth measurement.
- Stirred liquid media provide even cell suspension.
Bacterial Colonies
- Visible clusters of bacteria resulting from growth on solid media.
- Colonies can be observed and characterized (e.g., in Petri dishes or blood agar).
Bacterial Colonies on Blood Agar
- Diagram illustrating bacterial colonies growing on blood agar media. Specific colonies include Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus (beta-hemolytic, and Staphylococcus epidermidis.
Growth and Reproduction in Natural Environments
- Nutrients limit indefinite bacterial reproduction in natural environments.
- Adaptations, like increased resource availability and community structures (like biofilms), enable persistence.
- Syntrophy in communities.
Bacterial Growth Curve: Four Phases
- Lag phase: Slow growth while cells adapt to new conditions.
- Log/exponential phase: Rapid growth where nutrients are plentiful.
- Stationary phase: Growth slows due to resource depletion.
- Death phase: Rate of death exceeds new cells formation when nutrients have run out.
Common Diseases
- Pathogenic bacteria cause diverse illnesses involving multiple species.
- Examples include tetanus, typhoid fever, diphtheria, syphilis, cholera, foodborne illnesses, leprosy, and tuberculosis.
Common Diseases - Other Organisms
- Some obligate intracellular parasites (e.g., Rickettsia and Chlamydia) cause diverse diseases, including typhus, Rocky Mountain spotted fever, pneumonia, and urinary tract infections.
- Some pathogens (e.g., Pseudomonas aeruginosa, Burkholderia species, Mycobacterium) are opportunistic; causing diseases mainly in those with impaired immunity.
- List a number of infections and their causing agents (e.g., Bacterial meningitis, pneumonia, infections of the upper respiratory tract, gastrointestinal issues).
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Description
Test your knowledge on bacterial genetics, cellular structures, and their functions. This quiz covers various topics, including plasmids, flagella, and Koch's postulates, that are essential for understanding microbial biology. Challenge yourself to see how well you know the primary roles of different bacterial components!