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Questions and Answers
What distinguishes plasmids from episomes?
What distinguishes plasmids from episomes?
Which of the following statements is true regarding bacterial chromosomes?
Which of the following statements is true regarding bacterial chromosomes?
What is the primary function of a gene within a DNA molecule?
What is the primary function of a gene within a DNA molecule?
How many genes can typically be found in a plasmid?
How many genes can typically be found in a plasmid?
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Which component is NOT typically associated with the genomes of prokaryotic organisms?
Which component is NOT typically associated with the genomes of prokaryotic organisms?
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What is the Gram stain result for the rod-shaped bacteria identified in the content?
What is the Gram stain result for the rod-shaped bacteria identified in the content?
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What is the cell shape of the Gram-positive bacteria mentioned in the content?
What is the cell shape of the Gram-positive bacteria mentioned in the content?
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Which of the following bacteria would be classified as Enterobacteriaceae?
Which of the following bacteria would be classified as Enterobacteriaceae?
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What is a key factor that influences binary fission in bacterial cells?
What is a key factor that influences binary fission in bacterial cells?
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How many different species of bacteria are mentioned in connection with different cell shapes and Gram results?
How many different species of bacteria are mentioned in connection with different cell shapes and Gram results?
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What is the process by which free DNA is transferred from one bacterial cell to another?
What is the process by which free DNA is transferred from one bacterial cell to another?
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Which method of genetic transfer involves a bacteriophage?
Which method of genetic transfer involves a bacteriophage?
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In which type of genetic transfer do mating cells physically contact each other?
In which type of genetic transfer do mating cells physically contact each other?
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Conjugation primarily occurs between which types of cells?
Conjugation primarily occurs between which types of cells?
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Which of the following statements accurately characterizes transformation?
Which of the following statements accurately characterizes transformation?
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What connection does conjugation have with antibiotic resistance?
What connection does conjugation have with antibiotic resistance?
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What is the role of the bacterium Yersinia pestis in the context of disease?
What is the role of the bacterium Yersinia pestis in the context of disease?
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What type of organism is Yersinia pestis classified as?
What type of organism is Yersinia pestis classified as?
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Which shape represents bacteria that are spherical or nearly spherical?
Which shape represents bacteria that are spherical or nearly spherical?
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What term describes paired cocci bacteria?
What term describes paired cocci bacteria?
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How do streptobacilli arrange themselves after division?
How do streptobacilli arrange themselves after division?
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Which of the following best describes the phenotypic characteristics of organisms?
Which of the following best describes the phenotypic characteristics of organisms?
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Which of the following domains have more known species?
Which of the following domains have more known species?
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Which type of bacteria is described as curved or comma-shaped rods?
Which type of bacteria is described as curved or comma-shaped rods?
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What are bacilli that have not separated after division called?
What are bacilli that have not separated after division called?
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Which classification is NOT a type of Archaea?
Which classification is NOT a type of Archaea?
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Which arrangement forms cocci bacteria in cubes of eight due to division in three planes?
Which arrangement forms cocci bacteria in cubes of eight due to division in three planes?
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What is the primary means of bacterial cell division?
What is the primary means of bacterial cell division?
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What distinguishes spirochetes from other spiral bacteria?
What distinguishes spirochetes from other spiral bacteria?
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When do some bacteria typically produce spores?
When do some bacteria typically produce spores?
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During the exponential growth phase, which of the following occurs?
During the exponential growth phase, which of the following occurs?
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What is a key characteristic of staphylococci bacteria?
What is a key characteristic of staphylococci bacteria?
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What do chemoheterotrophs require for survival?
What do chemoheterotrophs require for survival?
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What is a characteristic of plasmids?
What is a characteristic of plasmids?
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What defines Archaea as extremophiles?
What defines Archaea as extremophiles?
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What characteristic is unique to Archaea compared to Bacteria?
What characteristic is unique to Archaea compared to Bacteria?
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Which of the following is true about the replication origins of Archaea?
Which of the following is true about the replication origins of Archaea?
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What type of lipid is found in the plasma membranes of Archaea?
What type of lipid is found in the plasma membranes of Archaea?
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Which of the following enzymes is not typically produced by Archaea?
Which of the following enzymes is not typically produced by Archaea?
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How do Archaea differ in initiator tRNA compared to Bacteria?
How do Archaea differ in initiator tRNA compared to Bacteria?
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Which statement about the environmental distribution of Archaea is correct?
Which statement about the environmental distribution of Archaea is correct?
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What is a key characteristic of the cell wall of Bacteria?
What is a key characteristic of the cell wall of Bacteria?
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What is the Gram classification of the bacteria exhibiting rod shapes in the Gram stain?
What is the Gram classification of the bacteria exhibiting rod shapes in the Gram stain?
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Which type of bacterial cell division results in two equal daughter cells?
Which type of bacterial cell division results in two equal daughter cells?
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Which description accurately characterizes the Gram-positive bacteria mentioned?
Which description accurately characterizes the Gram-positive bacteria mentioned?
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How many different species of bacteria are present when one is Gram-positive and the other is Gram-negative?
How many different species of bacteria are present when one is Gram-positive and the other is Gram-negative?
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What distinguishes the Enterobacteriaceae mentioned from other bacterial families?
What distinguishes the Enterobacteriaceae mentioned from other bacterial families?
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Which of the following describes diplobacilli?
Which of the following describes diplobacilli?
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What arrangement of cocci forms grape-like clusters?
What arrangement of cocci forms grape-like clusters?
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How do spirillum bacteria differ from other spiral bacteria?
How do spirillum bacteria differ from other spiral bacteria?
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Which of the following describes staphylococci arrangements?
Which of the following describes staphylococci arrangements?
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What describes pleomorphic bacteria?
What describes pleomorphic bacteria?
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Which of the following shapes is classified as vibrios?
Which of the following shapes is classified as vibrios?
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What term refers to chains of cocci bacteria?
What term refers to chains of cocci bacteria?
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Which bacteria arrangement is known for forming cubes of eight?
Which bacteria arrangement is known for forming cubes of eight?
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What is the primary process by which free DNA is taken up by a bacterial cell from its environment?
What is the primary process by which free DNA is taken up by a bacterial cell from its environment?
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Which genetic transfer method involves the use of a bacteriophage?
Which genetic transfer method involves the use of a bacteriophage?
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Conjugation in prokaryotes primarily requires what key condition?
Conjugation in prokaryotes primarily requires what key condition?
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In what context is bacterial conjugation particularly significant?
In what context is bacterial conjugation particularly significant?
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Which of the following statements best describes transformation?
Which of the following statements best describes transformation?
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During which genetic transfer process can bacteria acquire traits such as antibiotic resistance?
During which genetic transfer process can bacteria acquire traits such as antibiotic resistance?
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Which component is critical for the process of transduction in bacteria?
Which component is critical for the process of transduction in bacteria?
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Which type of bacterial genetic transfer allows for DNA exchange between closely related species?
Which type of bacterial genetic transfer allows for DNA exchange between closely related species?
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What is a disadvantage of using dry weight measurement for estimating cell mass?
What is a disadvantage of using dry weight measurement for estimating cell mass?
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During which phase of the population growth curve do bacteria adapt to their environment?
During which phase of the population growth curve do bacteria adapt to their environment?
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Which method counts only viable bacterial cells?
Which method counts only viable bacterial cells?
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What happens during the stationary phase of bacterial growth?
What happens during the stationary phase of bacterial growth?
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What is an advantage of measuring turbidity in bacterial growth?
What is an advantage of measuring turbidity in bacterial growth?
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Which of the following statements correctly describes spores?
Which of the following statements correctly describes spores?
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What primarily influences the rate of binary fission in bacteria?
What primarily influences the rate of binary fission in bacteria?
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What occurs at the beginning of the death phase in bacterial growth?
What occurs at the beginning of the death phase in bacterial growth?
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What primary characteristic differentiates Archaea from bacteria?
What primary characteristic differentiates Archaea from bacteria?
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What is the classification level below Kingdom?
What is the classification level below Kingdom?
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Which metabolic condition do thermophiles thrive in?
Which metabolic condition do thermophiles thrive in?
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What describes the arrangement of bacteria that form clusters due to division in multiple planes?
What describes the arrangement of bacteria that form clusters due to division in multiple planes?
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What term is used for the structural pattern formed when bacilli do not separate after division?
What term is used for the structural pattern formed when bacilli do not separate after division?
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In which environment are halophiles primarily found?
In which environment are halophiles primarily found?
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Which process is responsible for the asexual reproduction of bacteria?
Which process is responsible for the asexual reproduction of bacteria?
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What is a primary characteristic of a plasmid?
What is a primary characteristic of a plasmid?
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What distinguishes the lipid composition of Archaea from that of Bacteria?
What distinguishes the lipid composition of Archaea from that of Bacteria?
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How are the chromosomes of Bacteria and Archaea typically structured?
How are the chromosomes of Bacteria and Archaea typically structured?
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What is a characteristic of Archaea that allows them to survive in extreme environments?
What is a characteristic of Archaea that allows them to survive in extreme environments?
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Which statement correctly compares the sensitivity of Bacteria and Archaea to streptomycin?
Which statement correctly compares the sensitivity of Bacteria and Archaea to streptomycin?
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What is the primary structural difference between the cell walls of Bacteria and Archaea?
What is the primary structural difference between the cell walls of Bacteria and Archaea?
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Which characteristic is exclusive to Archaea in comparison to Bacteria?
Which characteristic is exclusive to Archaea in comparison to Bacteria?
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What is a significant challenge in studying Archaea in laboratory settings?
What is a significant challenge in studying Archaea in laboratory settings?
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Which of the following best defines an extremophile?
Which of the following best defines an extremophile?
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Study Notes
Lecture 3: Bacteria and Archaea
- Lecture presented by Dr. Claire Atkinson
- Learning objectives included understanding bacterial structures, growth, genetics, archaea, and their differences from bacteria.
Learning Objectives
- Identify the shapes and arrangements of bacteria (cocci, bacilli, vibrio, spirilla, pleomorphic, spirochetes, diplo, staph, strep, palisades, tetrads, and sarcina)
- Describe bacterial growth
- Explain bacterial genetics (genome, chromosome, gene, plasmids, episomes)
- Define archaea
- Contrast archaea with bacteria
Bacterial Cell Morphology
- Bacteria exhibit diverse shapes:
- Coccus (spherical)
- Bacillus (rod-shaped)
- Vibrio (curved or comma-shaped)
- Spirilla (spiral-shaped)
- Spirochetes (thin, flexible spirals)
- Pleomorphic (variable shapes)
- Arrangement after cell division affects the appearance of colonies:
- Diplococci (pairs)
- Streptococci (chains)
- Tetrads (groups of four)
- Staphylococci (clusters)
- Sarcina (cubes of eight)
Bacterial Growth
- Binary fission is the primary method of bacterial cell division.
- Growth phases are characterized by the number of cells in each phase:
- Lag phase: Cells adapt to the medium before division.
- Log phase: Cell growth increases exponentially.
- Stationary phase: Essential nutrients are depleted or byproducts accumulate.
- Death phase: Number of dead cells exceeds viable cells.
Measuring Bacterial Growth
- Methods measure cell mass or count viable cells.
- Dry weight (most accurate but slow)
- Turbidity (cloudiness, measured using spectrophotometer.)
- Direct cell count (microscopic counts in counting chambers)
- Viable counts (only live cells are counted, techniques like pour plating, spread plating)
Bacterial Genetics
- Genome: Genetic material of an organism.
- Chromosome: Single DNA molecule in bacteria.
- Gene: Unit of genetic information on DNA
- Plasmid: Small, circular DNA molecule, independent of the chromosome.
- Plasmids can have genes that provide antibiotic resistance.
- Episome: DNA that can integrate itself into the bacterial chromosome.
Bacterial Genetic Transfer
- Transformation: Transfer of free DNA from one cell to another.
- Transduction: Transfer of bacterial DNA from a bacteriophage to another bacterium.
- Conjugation: Gene transfer between mating cells.
Archaea
- Are prokaryotes.
- Often live in extreme environments (extremeophiles) — such as hot springs, volcanic vents, or highly salty environments.
- Are widely distributed in various environments alongside bacteria
- Many archaea species are used in industrial processes such as bioremediation
- Many bacterial enzymes are derived from archaea for many industrial processes.
- Not much known about archaea species due to difficulty growing many types in laboratory settings.
- Not linked to human diseases in any well-documented way.
Bacteria and Archaea Differences
- Archaea cell walls do not contain peptidoglycan.
- Bacteria use ester linkages and archaea use ether linkages to attach their phospholipids.
- Bacterial ribosomes are more sensitive to streptomycin than archaea ribosomes.
- Bacterial and archaea membranes have differences in their lipids.
- Bacterial chromosomes and genes have different properties/composition than archaea chromosomes and genes.
Identifying Bacteria
- Phenotypical methods like shape, color, and size
- Analytical methods like analyzing cell components
- Genotypic methods like genetic information (DNA, rRNA)
Taxonomy
- The science of classifying organisms.
- Formal system of organizing organisms by categories.
- Can classify archaea into categories similarly to bacteria such as domains, kingdom, phyla, and so on.
Quiz Time
- Review questions relating to cells, morphology, arrangements, arrangement after division, methods for testing, growth curves (lag, log, stationary, and death phases), antibiotics, genetic material, and bacteria vs archaea.
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Description
Test your knowledge of bacterial genetics, including plasmids, chromosomes, and genetic transfer mechanisms. This quiz covers essential concepts related to bacterial cell structure and the classification of bacteria. Challenge yourself to see how much you know about the fascinating world of prokaryotic organisms.