Bacteria
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Questions and Answers

What is a common feature of all bacteria?

  • They have membrane-bound organelles.
  • They lack chlorophyll. (correct)
  • They contain multiple linear chromosomes.
  • They are multicellular organisms.
  • What distinguishes Gram-positive bacteria from Gram-negative bacteria?

  • Gram-positive bacteria have a thick peptidoglycan cell wall. (correct)
  • Gram-positive bacteria are classified based on oxygen requirements.
  • Gram-positive bacteria have a thin peptidoglycan cell wall.
  • Gram-positive bacteria have a thick lipopolysaccharide layer.
  • In the scientific naming of bacteria, what is the correct format for 'Streptococcus pyogenes'?

  • Streptococcus pyogenes (correct)
  • streptococcus pyogenes
  • STREPTOCOCCUS PYOGENES
  • Streptococcus Pyogenes
  • What is the primary purpose of Gram staining?

    <p>To classify bacterial species into two groups.</p> Signup and view all the answers

    What environmental factor is NOT typically considered for bacteria cultivation?

    <p>Chlorophyll content</p> Signup and view all the answers

    Which part of the bacterial structure is responsible for movement?

    <p>Flagellum</p> Signup and view all the answers

    What is the classification hierarchy that includes 'Firmicutes'?

    <p>Division</p> Signup and view all the answers

    Which of the following is NOT a method for classifying bacteria?

    <p>Color of the bacteria</p> Signup and view all the answers

    Which of the following structures is commonly found in bacteria?

    <p>Cell wall</p> Signup and view all the answers

    Study Notes

    Features of bacteria

    • Bacteria are small, single-celled microorganisms
    • They are an ancient lifeform
    • They possess the same basic functions as other living cells but lack chlorophyll
    • They usually have a single circular DNA chromosome found within the cytoplasm
    • They do not have membrane-bound organelles

    Size of Bacteria

    • Bacteria sizes are generally measured in micrometers (µm)
    • A micrometer is one millionth of a meter

    Shape of Bacteria

    • There are three main shapes of bacteria:
      • Cocci (spherical)
      • Bacilli (rod-shaped)
      • Spirilla (spiral shaped)

    Arrangement of Bacteria

    • Cocci can be arranged in pairs (diplococci), chains (streptococci), clusters (staphylococci), or cubical packets (sarcinae)
    • Bacilli can be arranged in pairs (diplobacilli), chains (streptobacilli), or palisades (side-by-side).

    Structure of Bacteria

    • Flagellum: A long, whip-like appendage responsible for bacterial motility
    • Ribosomes: Small organelles responsible for protein synthesis
    • Cytoplasm: The jelly-like substance that fills the cell
    • Nucleoid: The region containing the bacterial DNA
    • Plasma membrane: The thin, outer layer that encloses the cytoplasm and controls the passage of substances into and out of the cell
    • Cell wall: A rigid structure that provides support and shape to the cell
    • Capsule: A sticky layer that surrounds the cell wall and helps bacteria adhere to surfaces, resist phagocytosis (being engulfed by white blood cells), and evade the host's immune system
    • Pilus: A thin, hair-like structure that helps bacteria attach to surfaces

    Gram Staining

    • Gram staining is a technique used to distinguish and classify bacterial species into two groups:
      • Gram-positive bacteria
      • Gram-negative bacteria
    • The technique was developed by the Danish bacteriologist Hans Christian Gram

    Gram Staining Explained

    • Gram-positive bacteria have a thick peptidoglycan layer in their cell walls, which retains the crystal violet dye, making them appear purple under a microscope. examples include S. aureus and S. pneumoniae.
    • Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane containing lipopolysaccharides, which does not retain the crystal violet dye as effectively. They are stained with a counterstain (usually safranin), which makes them appear pink under a microscope. Examples include E. coli and P. aeruginosa.

    Environmental Requirements

    • There are a variety of environmental factors that can influence the growth and survival of bacteria, including:
      • Moisture: Water is essential for bacterial growth and metabolism.
      • Oxygen: Some bacteria require oxygen for growth (aerobes), while others cannot tolerate oxygen (anaerobes) and some can tolerate oxygen but grow better without it (facultative anaerobes)
      • Carbon: Carbon is the backbone of all organic molecules and is a necessary nutrient for bacterial growth.
      • Dioxide: Carbon dioxide is a byproduct of respiration, and some bacteria require it for growth.
      • Temperature: Bacteria have optimal temperature ranges for growth, with some bacteria preferring cold temperatures (psychrophiles), others warm temperatures (mesophiles), and a few heat-loving bacteria (thermophiles).
      • pH: Each bacterial species has a preferred pH range for growth, with some thriving in acidic or alkaline conditions.
        • Osmotic effect: Water and nutrients move in and out of bacterial cells by osmosis.
        • Bacteria need to maintain a balance for osmotic pressure, if a bacterial cell is in a solution that is too high or too low in salt concentration, the osmotic pressure could cause the cell to shrink or burst.
        • Mechanical stress: Bacteria can be affected by mechanical stress, such as forces caused by moving liquids or solids, which can damage or kill them.

    Naming Bacteria

    • The systematic classification of organisms into ordered groups is called taxonomy.
    • The name is typically a combination of the last two ranks, genus and species.
    • The species name does not begin with a capital letter.
    • The scientific name is usually written in italics.
    • Generic names can be abbreviated, for example, S. aureus can be abbreviated as S. aureus.
    • S. aureus refers to Staphylococcus aureus.

    Classification of Organisms

    • Kingdom: Procaryote
    • Division: Firmicutes
    • Subdivision: Low DNA content
    • Family: Streptococcaceae
    • Genus: Streptococcus
    • Species: Streptococcus mutans

    Bacteria in Dentistry

    • Streptococcus mutans and Streptococcus sanguis are examples of bacteria associated with dentistry.
    • Streptococcus mutans is a key player in the formation of dental caries (tooth decay) and is responsible for the production of lactic acid, which erodes tooth enamel.
    • Streptococcus sanguis is typically found in plaque and contributes to the development of dental plaque, but generally plays a less significant role in tooth decay compared to Streptococcus mutans.
    • Streptococcus sanguinis may even play a protective role in oral health under certain conditions.

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