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Questions and Answers
What is a common feature of all bacteria?
What is a common feature of all bacteria?
What distinguishes Gram-positive bacteria from Gram-negative bacteria?
What distinguishes Gram-positive bacteria from Gram-negative bacteria?
In the scientific naming of bacteria, what is the correct format for 'Streptococcus pyogenes'?
In the scientific naming of bacteria, what is the correct format for 'Streptococcus pyogenes'?
What is the primary purpose of Gram staining?
What is the primary purpose of Gram staining?
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What environmental factor is NOT typically considered for bacteria cultivation?
What environmental factor is NOT typically considered for bacteria cultivation?
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Which part of the bacterial structure is responsible for movement?
Which part of the bacterial structure is responsible for movement?
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What is the classification hierarchy that includes 'Firmicutes'?
What is the classification hierarchy that includes 'Firmicutes'?
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Which of the following is NOT a method for classifying bacteria?
Which of the following is NOT a method for classifying bacteria?
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Which of the following structures is commonly found in bacteria?
Which of the following structures is commonly found in bacteria?
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Study Notes
Features of bacteria
- Bacteria are small, single-celled microorganisms
- They are an ancient lifeform
- They possess the same basic functions as other living cells but lack chlorophyll
- They usually have a single circular DNA chromosome found within the cytoplasm
- They do not have membrane-bound organelles
Size of Bacteria
- Bacteria sizes are generally measured in micrometers (µm)
- A micrometer is one millionth of a meter
Shape of Bacteria
- There are three main shapes of bacteria:
- Cocci (spherical)
- Bacilli (rod-shaped)
- Spirilla (spiral shaped)
Arrangement of Bacteria
- Cocci can be arranged in pairs (diplococci), chains (streptococci), clusters (staphylococci), or cubical packets (sarcinae)
- Bacilli can be arranged in pairs (diplobacilli), chains (streptobacilli), or palisades (side-by-side).
Structure of Bacteria
- Flagellum: A long, whip-like appendage responsible for bacterial motility
- Ribosomes: Small organelles responsible for protein synthesis
- Cytoplasm: The jelly-like substance that fills the cell
- Nucleoid: The region containing the bacterial DNA
- Plasma membrane: The thin, outer layer that encloses the cytoplasm and controls the passage of substances into and out of the cell
- Cell wall: A rigid structure that provides support and shape to the cell
- Capsule: A sticky layer that surrounds the cell wall and helps bacteria adhere to surfaces, resist phagocytosis (being engulfed by white blood cells), and evade the host's immune system
- Pilus: A thin, hair-like structure that helps bacteria attach to surfaces
Gram Staining
- Gram staining is a technique used to distinguish and classify bacterial species into two groups:
- Gram-positive bacteria
- Gram-negative bacteria
- The technique was developed by the Danish bacteriologist Hans Christian Gram
Gram Staining Explained
- Gram-positive bacteria have a thick peptidoglycan layer in their cell walls, which retains the crystal violet dye, making them appear purple under a microscope. examples include S. aureus and S. pneumoniae.
- Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane containing lipopolysaccharides, which does not retain the crystal violet dye as effectively. They are stained with a counterstain (usually safranin), which makes them appear pink under a microscope. Examples include E. coli and P. aeruginosa.
Environmental Requirements
- There are a variety of environmental factors that can influence the growth and survival of bacteria, including:
- Moisture: Water is essential for bacterial growth and metabolism.
- Oxygen: Some bacteria require oxygen for growth (aerobes), while others cannot tolerate oxygen (anaerobes) and some can tolerate oxygen but grow better without it (facultative anaerobes)
- Carbon: Carbon is the backbone of all organic molecules and is a necessary nutrient for bacterial growth.
- Dioxide: Carbon dioxide is a byproduct of respiration, and some bacteria require it for growth.
- Temperature: Bacteria have optimal temperature ranges for growth, with some bacteria preferring cold temperatures (psychrophiles), others warm temperatures (mesophiles), and a few heat-loving bacteria (thermophiles).
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pH: Each bacterial species has a preferred pH range for growth, with some thriving in acidic or alkaline conditions.
- Osmotic effect: Water and nutrients move in and out of bacterial cells by osmosis.
- Bacteria need to maintain a balance for osmotic pressure, if a bacterial cell is in a solution that is too high or too low in salt concentration, the osmotic pressure could cause the cell to shrink or burst.
- Mechanical stress: Bacteria can be affected by mechanical stress, such as forces caused by moving liquids or solids, which can damage or kill them.
Naming Bacteria
- The systematic classification of organisms into ordered groups is called taxonomy.
- The name is typically a combination of the last two ranks, genus and species.
- The species name does not begin with a capital letter.
- The scientific name is usually written in italics.
- Generic names can be abbreviated, for example, S. aureus can be abbreviated as S. aureus.
- S. aureus refers to Staphylococcus aureus.
Classification of Organisms
- Kingdom: Procaryote
- Division: Firmicutes
- Subdivision: Low DNA content
- Family: Streptococcaceae
- Genus: Streptococcus
- Species: Streptococcus mutans
Bacteria in Dentistry
- Streptococcus mutans and Streptococcus sanguis are examples of bacteria associated with dentistry.
- Streptococcus mutans is a key player in the formation of dental caries (tooth decay) and is responsible for the production of lactic acid, which erodes tooth enamel.
- Streptococcus sanguis is typically found in plaque and contributes to the development of dental plaque, but generally plays a less significant role in tooth decay compared to Streptococcus mutans.
- Streptococcus sanguinis may even play a protective role in oral health under certain conditions.
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