Bacterial DNA Replication Process
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Questions and Answers

Who invented the microscope and described the first microbes as the fruiting structures of fungi?

  • Antonie van Leeuwenhoek (correct)
  • Louis Pasteur
  • Francesco Redi
  • Lazzaro Spallanzani

Which scientist conducted the meat and flies experiment, leading to the disproval of the idea of spontaneous generation for large organisms?

  • Louis Pasteur
  • Antonie van Leeuwenhoek
  • Francesco Redi (correct)
  • Robert Hooke

Who failed to grow microbes in sterilized meat broth by boiling and not allowing air access?

  • Francesco Redi
  • Robert Hooke
  • Louis Pasteur (correct)
  • Antonie van Leeuwenhoek

Which scientist used the Swan-necked flask experiment to disprove spontaneous generation?

<p>Louis Pasteur (B)</p> Signup and view all the answers

What was the main purpose of Francesco Redi's meat and flies experiment?

<p>To disprove the idea of spontaneous generation for large organisms (A)</p> Signup and view all the answers

What is the main characteristic of endosymbiotic organisms, like mitochondria?

<p>They live inside of another organism (B)</p> Signup and view all the answers

Which of the following is a characteristic of bacterial genomes?

<p>They have little non-coding DNA (D)</p> Signup and view all the answers

What is the function of the DNA control sequence in regulating gene expression?

<p>Regulates transcription of structural genes (A)</p> Signup and view all the answers

What is the main function of the nucleoid in bacterial cells?

<p>Contains multiple loops of chromosomes (D)</p> Signup and view all the answers

What are genes that have shared ancestry known as?

<p>Homologs (A)</p> Signup and view all the answers

Which process involves DNA uptake from the environment and can be induced by stress?

<p>Transformation (C)</p> Signup and view all the answers

What enhances the competence of bacteria cells for DNA transfer in Gram-negative bacteria?

<p>$CaCl_2$ and low temperature (C)</p> Signup and view all the answers

What is the fate of DNA entering a bacterial cell?

<p>It undergoes recombination with the recipient's DNA (A)</p> Signup and view all the answers

What are the specific recombination proteins required for site-specific recombination?

<p>Recombinase enzymes (B)</p> Signup and view all the answers

What type of transduction can transfer any gene from a donor to a recipient cell?

<p>Generalized transduction (D)</p> Signup and view all the answers

Which process involves cell-cell contact and is initiated by a pilus protruding from the donor cell?

<p>Conjugation (A)</p> Signup and view all the answers

What do bacterial colonies represent?

<p>The progeny of one original cell (A)</p> Signup and view all the answers

What did Angelina & Walther Hesse discover?

<p>A solid medium using agar for bacterial culture growth (B)</p> Signup and view all the answers

According to Robert Koch's Postulates, what is the first requirement to prove that a specific type of microorganism causes a specific disease?

<p>The suspect organism should be present in all cases of disease &amp; absent from healthy (C)</p> Signup and view all the answers

What is the Miller-Urey Experiment aimed to simulate?

<p>Chemical and energy conditions of prehistoric Earth's 'water cycle' (A)</p> Signup and view all the answers

What is the function of a molecular clock in evolutionary biology?

<p>To determine the genetic relatedness between species (C)</p> Signup and view all the answers

What is the Shine-Dalgarno sequence responsible for?

<p>Allowing binding of mRNA to ribosomes (A)</p> Signup and view all the answers

Who discovered that prokaryotes lived in hot springs and produced methane?

<p>Carl Woese (C)</p> Signup and view all the answers

What is phylogenetics the study of?

<p>Evolutionary relationships among biological individuals (B)</p> Signup and view all the answers

What is a common characteristic of archaea and bacteria?

<p>Similar size and shape (C)</p> Signup and view all the answers

What is a limitation of Robert Koch's Postulates?

<p>Slow growing pathogens/pathogens may fail postulate (D)</p> Signup and view all the answers

What was the aim of the Miller-Urey Experiment?

<p>To simulate chemical and energy conditions of prehistoric Earth's 'water cycle' (D)</p> Signup and view all the answers

What are stromatolites?

<p>Fossilized evidence of bacterial communities (D)</p> Signup and view all the answers

Which enzyme recognizes and repairs bases that mostly do not distort DNA structure?

<p>Methyl mismatch repair (B)</p> Signup and view all the answers

What is the function of RecA in the SOS response?

<p>Activates protease/self-break of LexA (B)</p> Signup and view all the answers

What is the role of Rho in transcription termination?

<p>Binds mRNA and moves along (C)</p> Signup and view all the answers

Which factor is needed for the initiation of RNA synthesis and recognizes -10 & -35 promoters?

<p>Sigma factor (C)</p> Signup and view all the answers

What is the function of the Shine-Dalgarno sequence in translation?

<p>Binds to 30s subunit (B)</p> Signup and view all the answers

In bacterial cells, what does FtsZ form?

<p>&quot;Z-ring&quot; in spherical cells (B)</p> Signup and view all the answers

What is the function of MreB in bacterial cells?

<p>&quot;Coil inside rod-shaped cells&quot; (D)</p> Signup and view all the answers

What is the function of CreS in bacterial cells?

<p>&quot;Forms &quot;Crescentin&quot; along the inner side of crescent-shaped bacteria&quot; (B)</p> Signup and view all the answers

What is the role of Hapnoids in bacterial cell membrane?

<p>Strengthening lipid membrane (B)</p> Signup and view all the answers

What is the function of Sacculus in bacterial cells?

<p>Rigid structure outside of phospholipid membrane (C)</p> Signup and view all the answers

What distinguishes Gram-negative bacteria from Gram-positive bacteria in terms of cell wall structure?

<p>Lipopolysaccharide (LPS) presence (A)</p> Signup and view all the answers

What is the function of DNA helicase in bacterial DNA replication?

<p>Unwinds the two strands of DNA (C)</p> Signup and view all the answers

What is the role of DNA polymerase III in bacterial DNA replication?

<p>Synthesizes new DNA strands (C)</p> Signup and view all the answers

What is the function of RNA primers in bacterial DNA replication?

<p>Produces RNA primers (C)</p> Signup and view all the answers

What enzyme removes the RNA primers in bacterial DNA replication?

<p>DNA polymerase I (C)</p> Signup and view all the answers

In plasmid replication, what does it mean if a plasmid replicates only with the chromosome?

<p>It is a stringent plasmid (C)</p> Signup and view all the answers

What is the purpose of the ParMRC system in plasmids?

<p>To maintain plasmids for self-maintenance (D)</p> Signup and view all the answers

What is the Ames Test used for?

<p>To test for mutagen strength (A)</p> Signup and view all the answers

What is the main function of excision repair in DNA repair?

<p>To remove damaged nucleotide(s) (B)</p> Signup and view all the answers

What type of mutation changes the open-reading frame of a gene?

<p>Frame-shift mutation (B)</p> Signup and view all the answers

What enzyme binds to a pyrimidine dimer and cleaves the cyclobutane ring upon exposure to UV radiation?

<p>Photolyase enzyme (B)</p> Signup and view all the answers

What type of mutagen includes compounds that interfere with DNA chemistry, many of which are carcinogens?

<p>Chemical mutagens (D)</p> Signup and view all the answers

What type of mutation changes a purine to a pyrimidine, or vice versa?

<p>Transversion (C)</p> Signup and view all the answers

What is the function of the glycocalyx?

<p>It forms a network of polysaccharides on the cell surface (C)</p> Signup and view all the answers

What is the primary function of a capsule in bacterial cells?

<p>To protect against pH, viral infection, and extracellular enzymes (D)</p> Signup and view all the answers

What is the role of transporters in the movement of molecules across the membrane?

<p>To allow diffusion of substrates across the membrane (B)</p> Signup and view all the answers

In which type of transport does the movement occur against the gradient and require energy?

<p>Primary active transport (B)</p> Signup and view all the answers

What is the main source of energy for the phosphotransferase system (PTS) in sugar transport?

<p>ATP hydrolysis (B)</p> Signup and view all the answers

What is the function of the Sec System in bacterial cells?

<p>Transport of unfolded proteins requiring ATP hydrolysis to move out (B)</p> Signup and view all the answers

What is the function of S-layers in Archaea and many bacteria?

<p>Layers of protein/glycoprotein that protect against pH, viral infection, extracellular enzymes. (B)</p> Signup and view all the answers

What is the role of ABC Transporters (ATP-Binding Cassette) in cellular processes?

<p>Substrate-specific proteins that escort specific substrates to transporters. (D)</p> Signup and view all the answers

In which type of secretion system do proteins have a signal tag telling secretion machinery to move them out?

<p>Sec System. (B)</p> Signup and view all the answers

What is the primary role of group translocation (phosphotransferase system) in bacterial cells?

<p>Sugar transport with modification by phosphorylation to maintain a gradient. (D)</p> Signup and view all the answers

What is the primary characteristic of microorganisms as mentioned in the historical background of microbiology?

<p>They are genetically and metabolically diverse (D)</p> Signup and view all the answers

Who observed the first single-celled organisms (bacteria)?

<p>Antonie van Leeuwenhoek (D)</p> Signup and view all the answers

What experiment involved sterilized meat broth by boiling, which led to the failure of microbial growth?

<p>The Lazzaro Spallanzani experiment (D)</p> Signup and view all the answers

What theory did Francesco Redi's meat/flies experiment disprove?

<p>Spontaneous generation for large organisms (B)</p> Signup and view all the answers

Who invented the microscope and described the first microbes as the fruiting structures of fungi?

<p>Robert Hooke (A)</p> Signup and view all the answers

What is the term for the theory that living creatures could arise without parents?

<p>Spontaneous Generation (B)</p> Signup and view all the answers

What did Louis Pasteur disprove with the Swan-necked flask experiment?

<p>Spontaneous generation of microorganisms (D)</p> Signup and view all the answers

What is the main purpose of Koch's Postulates?

<p>To prove that a specific bacterium causes a specific disease (B)</p> Signup and view all the answers

What is the main limitation of Koch's Postulates?

<p>Slow-growing pathogens may fail the postulate (C)</p> Signup and view all the answers

What is the Metabolist Model primarily about?

<p>Formation of cellular metabolism from abiotic chemical reactions (D)</p> Signup and view all the answers

What is the function of RNA in some viruses?

<p>Has catalytic activity and synthesizes proteins in ribosomes (B)</p> Signup and view all the answers

What is the main purpose of a molecular clock or evolutionary chronometer?

<p>To measure a species' genome mutation rate and genetic relatedness (B)</p> Signup and view all the answers

What does the Shine-Dalgarno sequence allow in mRNA?

<p>Allows binding to the 30s subunit in bacterial cells (D)</p> Signup and view all the answers

What did Carl Woese discover through his analysis of 16S rRNA?

<p>Distinct form of life in hot springs producing methane (D)</p> Signup and view all the answers

What is the focus of phylogenetics?

<p>Study of evolutionary relationships among biological individuals (C)</p> Signup and view all the answers

During bacterial DNA replication, which enzyme is responsible for unwinding the two strands of DNA?

<p>DNA helicase (A)</p> Signup and view all the answers

What is the term for the process where a DNA molecule replicates in a semi-conservative manner?

<p>Semiconservative replication (C)</p> Signup and view all the answers

Which enzyme synthesizes new DNA strands during bacterial DNA replication?

<p>DNA polymerase III (C)</p> Signup and view all the answers

What is the enzyme that removes the RNA primers during the completion of DNA replication?

<p>DNA polymerase I (B)</p> Signup and view all the answers

Which mutation type involves a change in a single base in the DNA sequence?

<p>Transition (C)</p> Signup and view all the answers

What is the main purpose of the Ames Test?

<p>Identify mutagens in bacterial cells (D)</p> Signup and view all the answers

Which structure is a protective and stabilizing layer made up of about 200 sugars?

<p>O-specific polysaccharide (A)</p> Signup and view all the answers

What is the main function of the proofreading mechanism during DNA replication?

<p>Detect and correct mismatches in the DNA sequence (C)</p> Signup and view all the answers

Which type of transport involves moving molecules from an area of low concentration to an area of high concentration, requiring energy?

<p>Active transport (A)</p> Signup and view all the answers

Which enzyme is responsible for cleaving the cyclobutane ring of a pyrimidine dimer upon exposure to UV radiation?

<p>Photolyase (A)</p> Signup and view all the answers

Which protein complex is used for the injection of toxins and harmful molecules into eukaryotic or bacterial cells?

<p>Type VI secretion system (C)</p> Signup and view all the answers

What type of mutagen includes compounds that change a base's structure and pairing characteristics?

<p>Chemical mutagen (C)</p> Signup and view all the answers

What is the primary energy source used in the phosphotransferase system (PTS) for sugar transport?

<p>Phosphoenolpyruvate (PEP) (C)</p> Signup and view all the answers

During bacterial DNA replication, what role do Single-stranded DNA binding proteins (SSBs) play?

<p>Keep the single DNA strands apart during DNA synthesis (A)</p> Signup and view all the answers

Which system is responsible for the transport of unfolded proteins requiring ATP hydrolysis to move out of the cell?

<p>Sec System (B)</p> Signup and view all the answers

What is the term used for the rapid accumulation of mutations due to exposure to mutagens or other factors?

<p>Hypermutation phenotype (B)</p> Signup and view all the answers

What is the function of S-layers in Archaea and many bacteria?

<p>Protection against pH (A)</p> Signup and view all the answers

Which type of transport involves movement of substances along a chemical gradient with no energy input required?

<p>Facilitated diffusion (B)</p> Signup and view all the answers

What process involves enzymatic cleavage of alien DNA by restriction endonucleases at specific sites?

<p>DNA restriction and modification (B)</p> Signup and view all the answers

Which statement is true about the diversity of microbial life?

<p>Archaea and bacteria share similar ribosomes and metabolic pathways (B)</p> Signup and view all the answers

What is the primary characteristic of endosymbiotic organisms like mitochondria?

<p>They have their own circular genomes and prokaryotic-like ribosomes (C)</p> Signup and view all the answers

What is the fate of most foreign DNA entering a bacterial cell?

<p>It degrades by restriction endonucleases in the recipient cell (D)</p> Signup and view all the answers

What process involves DNA uptake from the environment and can be induced by stress?

<p>Transformation (D)</p> Signup and view all the answers

What is the function of operons in bacteria?

<p>To regulate expression of structural genes through DNA control sequences (B)</p> Signup and view all the answers

What is the primary role of a pilus in bacterial cells?

<p>To transfer DNA from one bacterium to another after cell-to-cell contact (A)</p> Signup and view all the answers

What distinguishes Gram-negative bacteria from Gram-positive bacteria in terms of DNA replication?

<p>Gram-negative bacteria replicate their circular chromosomes differently (B)</p> Signup and view all the answers

What is the function of genomic islands in bacterial genomes?

<p>To produce metabolites for the bacterial cell (C)</p> Signup and view all the answers

What type of gene transfer involves cell-cell contact and is initiated by a pilus protruding from the donor cell?

<p>Conjugation (C)</p> Signup and view all the answers

What is the primary function of horizontal gene transfer in bacteria?

<p>To acquire new genes from the environment through transformation (D)</p> Signup and view all the answers

What is the main role of genomic islands in bacterial genomes?

<p>To encode harmful genes that cause disease (D)</p> Signup and view all the answers

What enhances the competence of bacteria cells for DNA transfer in Gram-negative bacteria?

<p>The physiological state of the cell during early log phase growth (C)</p> Signup and view all the answers

Which repair mechanism recognizes damage that causes distortion in DNA structure and involves an endonuclease removing a patch of single-stranded DNA containing damaged bases?

<p>Nucleotide excision repair (B)</p> Signup and view all the answers

What is the role of RecA in the SOS response?

<p>Activates protease to break down LexA (A)</p> Signup and view all the answers

Which enzyme is responsible for synthesizing RNA by adding complementary bases to the template strand?

<p>RNA polymerase (B)</p> Signup and view all the answers

What is the primary function of the bacterial cell wall?

<p>Providing structural support and withstanding turgor pressure (A)</p> Signup and view all the answers

What distinguishes Gram-negative bacteria from Gram-positive bacteria in terms of cell wall structure?

<p>Absence of an outer membrane (B)</p> Signup and view all the answers

What is the main function of FtsZ in bacterial cells?

<p>Forming a structural ring in spherical cells (D)</p> Signup and view all the answers

What molecule strengthens the lipid membrane in bacterial cells?

<p>Hapanoids (C)</p> Signup and view all the answers

Which transcription termination pathway involves the formation of a GC stem loop and subsequent destabilization at the poly-U site?

<p>Rho-independent pathway (B)</p> Signup and view all the answers

Which molecule serves as a ribosome-binding site on mRNA, allowing binding to the 30s subunit during translation?

<p>Shine-Dalgarno sequence (B)</p> Signup and view all the answers

Which enzyme removes damaged nucleotide(s) during excision repair mechanisms?

<p>Endonuclease (A)</p> Signup and view all the answers

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