Bacterial Culture Media Overview

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Questions and Answers

What is the primary purpose of using McConkey agar?

To isolate Staphylococcus species
To culture non-fastidious organisms
To test antibiotic susceptibility
To differentiate between Gram-negative and Gram-positive bacteria (correct)

Which agar is primarily used to perform antibiotic susceptibility tests?

Phenylethyl alcohol agar
Onoz agar
Nutrient agar
Mueller-Hinton agar (correct)

What is a distinguishing feature of Nutrient agar?

It is used to isolate Gram-positive bacteria.
It is used for rapid diagnosis of Salmonella and Shigella.
It does not selectively grow pathogenic bacteria. (correct)
It selectively grows pathogenic bacteria.

Onoz agar is specifically used for diagnosing which of the following?

<p>Salmonella and Shigella species (D)</p> Signup and view all the answers

Which agar is effective for isolating Staphylococcus species while inhibiting Gram-negative bacilli?

<p>Phenylethyl alcohol agar (B)</p> Signup and view all the answers

Flashcards

Phenylethyl alcohol agar (PEA)

A selective medium used to isolate Staphylococcus species by inhibiting the growth of Gram-negative bacilli, such as E. coli, Shigella, and Proteus.

Onoz agar

A medium used for rapid diagnosis of Salmonella and Shigella species by differentiating them from other Enterobacteriaceae family members.

Nutrient agar

A medium used to culture non-fastidious organisms, observing their pigment production. It's known for its safety as it does not promote the growth of pathogenic bacteria.

McConkey agar (MAC)

A selective medium that inhibits the growth of Gram-positive bacteria, allowing for the differentiation of Gram-negative and Gram-positive organisms.

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Mueller-Hinton agar (MHA)

A medium specifically designed for testing antibiotic susceptibility of bacteria.

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Study Notes

Bacterial Culture Media

McConkey agar (MAC) differentiates Gram-negative (Gm-) and Gram-positive (Gm+) bacteria by inhibiting the growth of Gram-positive bacteria.
Mueller-Hinton agar (MHA) is used for testing antibiotic susceptibility.
Nutrient agar is used for culturing non-fastidious organisms and observing pigment production. It's considered a safe medium because it doesn't preferentially grow pathogenic bacteria.
Onoz agar aids in the rapid diagnosis of Salmonella and Shigella, distinguishing them from other Enterobacteriaceae family members.
Phenylethyl alcohol agar (PEA) isolates Staphylococcus species by inhibiting the growth of Gram-negative bacilli, such as E. coli, Shigella, and Proteus.