32 Questions
What is the method that allows the multiplication of bacterial cells in or on a controlled culture medium?
Bacterial culture
What is the gel-like product of certain seaweeds used for solidifying certain culture media?
Agar agar
How long does it take for a single bacterium to reach millions in number under favorable conditions?
10-12 hours
What are the essential nutrients and minerals provided by culture media to support the growth of microorganisms?
Vitamins and minerals
At what temperature does agar agar become solid after being in liquid form?
40°C
How do bacteria grow on solid media?
As colonies
What is the main characteristic that defines a bacterial colony?
Size
Which phase of the bacterial growth curve is characterized by a waiting period/phase?
Lag phase
What is the primary oxygen requirement of facultative anaerobic bacteria?
Aerotolerant
What is the most common pH range for bacterial growth?
6.5-7.5
Which substance is added to some culture media to enrich it and determine the hemolytic activity of microorganisms?
Erythrocytes
What are the primary chemicals required for bacterial growth in the environment?
Carbon, nitrogen, minerals, and water
Which type of bacteria can reproduce in an environment with low concentrations of O2?
Microaerophile
What is the primary characteristic used for the identification of bacteria?
Staining properties
What is the main purpose of adding blood to culture media in determining bacterial hemolytic activity?
To enrich the medium and determine hemolytic activity
Which phase of the bacterial growth curve is characterized by a period of death or decline?
Period of decline
What is the primary purpose of bacterial culture?
To allow the multiplication of bacterial cells under controlled laboratory conditions
Which substance is used to solidify certain culture media?
Agar agar
What is the temperature at which agar agar becomes solid after being in liquid form?
40°C
What is the main characteristic that defines a bacterial colony?
Bacteria grow as colonies on solid media
Which phase of the bacterial growth curve is characterized by a period of rapid multiplication?
Log phase
What are the culture media used in bacterial culture primarily designed to provide?
Energy sources for bacteria
What is the primary purpose of adding blood to culture media in determining bacterial hemolytic activity?
To enrich the medium and visualize hemolytic activity of the microorganism
Which phase of the bacterial growth curve is characterized by a period of death or decline?
Stationary phase
What are the primary chemicals required for bacterial growth in the environment?
Carbon and nitrogen
What is the main characteristic used for the identification of bacteria?
Morphological features
At what temperature does agar agar become solid after being in liquid form?
60°C - 80°C
Which type of bacteria can reproduce in an environment with low concentrations of O2?
Microaerophile
What is the method that allows the multiplication of bacterial cells in or on a controlled culture medium?
Batch culture
What is the most common pH range for bacterial growth?
6.5-7.5
What is the main purpose of sterilizing the culture medium?
To eliminate unwanted contaminants that could inhibit bacterial growth
What is the primary oxygen requirement of facultative anaerobic bacteria?
Unaffected by the presence or absence of O2 in the breeding environment
Test your knowledge of bacterial growth and culture with this quiz. Learn about the conditions required for bacterial replication and the process of bacterial doubling in a culture medium.
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