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Questions and Answers
What is the main characteristic of Gram-positive bacteria?
What is the main characteristic of Gram-positive bacteria?
Which type of bacteria obtains energy by consuming other organisms?
Which type of bacteria obtains energy by consuming other organisms?
What is the function of the cell membrane?
What is the function of the cell membrane?
Which organelle is responsible for protein synthesis?
Which organelle is responsible for protein synthesis?
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What is the function of flagella?
What is the function of flagella?
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What is the main function of the capsule?
What is the main function of the capsule?
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What division does Escherichia coli belong to?
What division does Escherichia coli belong to?
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What is the order of Escherichia coli in the Adansonian classification?
What is the order of Escherichia coli in the Adansonian classification?
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What is the basis of genetic or molecular classification?
What is the basis of genetic or molecular classification?
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What is the outermost layer of a bacterium composed of?
What is the outermost layer of a bacterium composed of?
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What is the function of slime layer protein?
What is the function of slime layer protein?
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What is the composition of slime layer?
What is the composition of slime layer?
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What is the role of fimbriae in bacteria?
What is the role of fimbriae in bacteria?
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What is the term for all substances containing polysaccharides outside the cell wall?
What is the term for all substances containing polysaccharides outside the cell wall?
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What kingdom do microorganisms belong to?
What kingdom do microorganisms belong to?
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What is the main difference between prokaryotes and eukaryotes?
What is the main difference between prokaryotes and eukaryotes?
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What is the function of bacterial classification?
What is the function of bacterial classification?
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Which type of classification is a type of hierarchical classification that represents a branching tree-like arrangement?
Which type of classification is a type of hierarchical classification that represents a branching tree-like arrangement?
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What is a characteristic of archaebacteria?
What is a characteristic of archaebacteria?
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What do bacteria lack, unlike blue green algae?
What do bacteria lack, unlike blue green algae?
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What is the basis of bacterial classification?
What is the basis of bacterial classification?
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What is the name of the approach that is usually followed in bacterial classification?
What is the name of the approach that is usually followed in bacterial classification?
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Study Notes
Bacterial Classification
- Based on cell wall composition:
- Gram-positive bacteria: thick peptidoglycan layer, no outer membrane
- Gram-negative bacteria: thin peptidoglycan layer, outer membrane with lipopolysaccharides
- Based on shape:
- Spherical (cocci)
- Rod-shaped (bacilli)
- Spiral (spirilla)
- Comma-shaped (vibrios)
- Based on metabolism:
- Phototrophic: use light energy
- Chemotrophic: use chemical energy
- Heterotrophic: obtain energy by consuming other organisms
- Based on oxygen requirements:
- Aerobic: require oxygen
- Anaerobic: do not require oxygen
- Facultative anaerobic: can grow with or without oxygen
Bacterial Structure
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Cell wall: provides shape, support, and protection
- Peptidoglycan (murein): thick, rigid layer in Gram-positive bacteria
- Outer membrane: thin, flexible layer in Gram-negative bacteria
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Cell membrane: regulates transport, maintains cell environment
- Phospholipid bilayer with embedded proteins
-
Cytoplasm: site of metabolic reactions
- Gel-like substance with water, salts, and organic molecules
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Nucleoid: region with genetic material
- No true nucleus, genetic material is not membrane-bound
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Ribosomes: site of protein synthesis
- Small, spherical organelles found throughout cytoplasm
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Flagella: used for motility
- Long, whip-like structures composed of protein
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Pili: used for adhesion and DNA transfer
- Short, hair-like structures composed of protein
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Capsule: protective layer outside cell wall
- Polysaccharide or protein layer that helps evade host immune system
Bacterial Classification
- Bacteria classified based on cell wall composition, shape, metabolism, and oxygen requirements
- Gram-positive bacteria: thick peptidoglycan layer, no outer membrane
- Gram-negative bacteria: thin peptidoglycan layer, outer membrane with lipopolysaccharides
Bacterial Shapes
- Spherical (cocci)
- Rod-shaped (bacilli)
- Spiral (spirilla)
- Comma-shaped (vibrios)
Bacterial Metabolism
- Phototrophic: use light energy
- Chemotrophic: use chemical energy
- Heterotrophic: obtain energy by consuming other organisms
Oxygen Requirements
- Aerobic: require oxygen
- Anaerobic: do not require oxygen
- Facultative anaerobic: can grow with or without oxygen
Bacterial Structure
Cell Wall
- Provides shape, support, and protection
- Peptidoglycan (murein): thick, rigid layer in Gram-positive bacteria
- Outer membrane: thin, flexible layer in Gram-negative bacteria
Cell Membrane
- Regulates transport, maintains cell environment
- Phospholipid bilayer with embedded proteins
Cytoplasm
- Site of metabolic reactions
- Gel-like substance with water, salts, and organic molecules
Nucleoid
- Region with genetic material
- No true nucleus, genetic material is not membrane-bound
Ribosomes
- Site of protein synthesis
- Small, spherical organelles found throughout cytoplasm
Flagella
- Used for motility
- Long, whip-like structures composed of protein
Pili
- Used for adhesion and DNA transfer
- Short, hair-like structures composed of protein
Capsule
- Protective layer outside cell wall
- Polysaccharide or protein layer that helps evade host immune system
Classification of Living Organisms
- All living beings can be classified into three kingdoms: Plant, Animal, and Protista.
- Microorganisms are a heterogeneous group of several distinct living structures of microscopic size, classified under the kingdom Protista.
Types of Microorganisms
- Prokaryotes: Bacteria and blue-green algae, unicellular, capable of performing essential life processes, lacking chlorophyll.
- Eukaryotes: Fungi, algae (except blue-green algae), protozoa, and slime moulds.
- Archaebacteria: Closely related to eukaryotes, do not include human pathogens.
Bacterial Classification
- Bacterial classification: arrangement of organisms into taxonomic groups based on phenotypic and genotypic similarities and differences.
- Three main approaches: phylogenetic, Adansonian, and genetic classifications.
Phylogenetic Classification
- Hierarchical classification representing a branching tree-like arrangement.
- Example: E. coli classification:
- Division: Protophyta
- Class: Schizomycetes
- Order: Eubacteriales
- Family: Enterobacteriaceae
- Tribe: Escherichiae
- Genus: Escherichia
- Species: coli
Adansonian Classification
- Based on all characteristics expressed at the time of study, giving equal weight to all measurable features.
Genetic Classification
- Based on homology of DNA base sequences of microorganisms, using DNA hybridization or recombination methods.
Bacterial Anatomy
- Outermost layer consists of a rigid cell wall and cytoplasmic membrane.
- Protoplasm includes cytoplasm, cytoplasmic inclusions (ribosomes, mesosomes, granules, vacuoles, and nuclear body).
- Some bacteria have a viscid layer (slime layer or capsule) and filamentous appendages (flagella and fimbriae).
Slime Layer
- Structured paracrystalline protein layer composed of a single kind of protein molecule, sometimes with carbohydrates attached.
- Resistant to proteolytic enzymes and protein-denaturing agents, protects the cell from wall-degrading enzymes and bacteriophages.
Capsule
- Gelatinous secretion outside the cell wall.
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Description
This quiz explores the different ways to classify bacteria, including cell wall composition, shape, and metabolism. Test your knowledge of gram-positive and gram-negative bacteria, as well as phototrophic, chemotrophic, and heterotrophic bacteria.