Microbiology_3_Bacterial Cell Structure
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Questions and Answers

What is the primary function of the peptidoglycan layer in bacteria?

  • To facilitate nutrient absorption
  • To synthesize proteins
  • To provide energy storage
  • To protect the cell from osmotic pressure (correct)
  • Which type of protein constitutes approximately 75% of membrane proteins in bacterial plasma membranes?

  • Integral proteins (correct)
  • Peripheral proteins
  • Amphipathic proteins
  • Cholesterol oxidases
  • What is the significance of Lipopolysaccharide (LPS) in Gram-negative bacteria?

  • It inhibits protein synthesis
  • It enhances motility
  • It replaces peptidoglycan in the cell wall
  • It contributes to structural integrity and can stimulate an immune response (correct)
  • Which of the following best describes the hydrophobic component of the plasma membrane?

    <p>Hydrophobic fatty acid tails pointing inward (D)</p> Signup and view all the answers

    How much of the cell weight does the peptidoglycan layer account for in Gram-positive bacteria?

    <p>Approximately 90% (D)</p> Signup and view all the answers

    What is the role of integral proteins in the plasma membrane?

    <p>To transport ions across the membrane (D)</p> Signup and view all the answers

    Which of the following components is NOT part of the Lipopolysaccharide structure?

    <p>Lipid bilayer (D)</p> Signup and view all the answers

    What type of flagella arrangement is characterized by a single flagellum at one end of the bacterium?

    <p>Monotrichous (A)</p> Signup and view all the answers

    What is the primary function of the bacterial cell wall made of peptidoglycan?

    <p>To provide structural shape and protect against osmotic lysis (A)</p> Signup and view all the answers

    Which statement correctly differentiates Gram-positive bacteria from Gram-negative bacteria?

    <p>Gram-positive bacteria possess a thick cell wall with a single plasma membrane. (B)</p> Signup and view all the answers

    Which component is NOT typically found within the structure of prokaryotic cells?

    <p>Nucleus (A)</p> Signup and view all the answers

    Which of the following correctly describes peptidoglycan?

    <p>A polymer of sugars and amino acids with alternating units of NAG and NAM (B)</p> Signup and view all the answers

    What advantage do bacterial cells have over eukaryotic cells concerning environmental adaptability?

    <p>They can metabolize a wider range of substrates. (B)</p> Signup and view all the answers

    Which flagellar arrangement involves flagella located all around the bacterial cell surface?

    <p>Peritrichous (D)</p> Signup and view all the answers

    What is the primary function of conjugative pili in bacteria?

    <p>Transfer of DNA between two bacteria (A)</p> Signup and view all the answers

    Which statement correctly differentiates a capsule from a slime layer in bacteria?

    <p>Capsules are permanently organized, while slime layers are loosely formed. (B)</p> Signup and view all the answers

    Which component of bacteria contains the genetic material and is not membrane-bound?

    <p>Nucleoid (A)</p> Signup and view all the answers

    What is the approximate size range of bacterial genomes compared to the human genome?

    <p>0.6-10 Mbp vs. 3000 Mbp (D)</p> Signup and view all the answers

    Which of the following statements about ribosomes in bacteria is accurate?

    <p>Bacterial ribosomes are targeted by antibiotics due to their structural differences. (A)</p> Signup and view all the answers

    What is the key characteristic of plasmids that distinguishes them from chromosomal DNA?

    <p>Plasmids can replicate independently within a host. (C)</p> Signup and view all the answers

    Which bacterial genera are most associated with endospore formation?

    <p>Bacillus and Clostridium (A)</p> Signup and view all the answers

    What is a characteristic feature of sporulation in bacteria?

    <p>Acts as a survival mechanism during adverse conditions (B)</p> Signup and view all the answers

    Which of the following best describes the structure of hyphae?

    <p>Tube-like filaments that can lack septa (B)</p> Signup and view all the answers

    What distinguishes bacteriophages from other viruses?

    <p>They primarily infect prokaryotes (B)</p> Signup and view all the answers

    Which statement is true regarding the organization of multicellular microorganisms?

    <p>Cells can remain attached after division (A)</p> Signup and view all the answers

    Which of the following is NOT a component of viral structures?

    <p>Cell membrane (A)</p> Signup and view all the answers

    Which bacterial structure is characterized by cells arranged in pairs?

    <p>Diplococcus (B)</p> Signup and view all the answers

    What term describes a bacterial arrangement of 8 cocci in a cuboidal form?

    <p>Sarcina (A)</p> Signup and view all the answers

    Which type of bacteria is identified as a single rod?

    <p>Bacillus (C)</p> Signup and view all the answers

    Which bacterial shape generally measures between 1 to 10µm and must be viewed under a microscope?

    <p>All of the above (D)</p> Signup and view all the answers

    Which arrangement consists of bacteria that form chains?

    <p>Streptobacilli (B)</p> Signup and view all the answers

    Which of the following is a characteristic feature of Spirellum bacteria?

    <p>Rigid spiral structure (A)</p> Signup and view all the answers

    Which type of cell arrangement is identified as having cells in groups of 4 in the same plane?

    <p>Tetrads (B)</p> Signup and view all the answers

    Which of these is NOT a common form of bacterial cell shapes?

    <p>Cubic (A)</p> Signup and view all the answers

    Flashcards

    Bacterial colonies

    Aggregates of individual bacteria, forming visible clusters.

    Bacterial Morphology

    The overall shape and form of a bacterial cell, determined by its cell wall and internal structures.

    Cocci

    Round-shaped bacteria, often arranged in various patterns like pairs, chains, or clusters.

    Bacilli

    Rod-shaped bacteria, varying in length and width, with some forming chains.

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    Spirillum

    Spiral-shaped bacteria, with a rigid structure, contributing to the overall bacterium shape.

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    Diplococcus

    A pairing of two cocci bacteria.

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    Streptococci

    A chain of cocci bacteria linked together.

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    Tetracoccus

    A group of four cocci bacteria arranged in a square.

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    Bacterial Cell Wall

    A major structural component of bacterial cells, primarily composed of peptidoglycan, providing shape and preventing osmotic lysis (bursting from internal pressure).

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    Gram-positive and Gram-negative bacteria

    A classification of bacteria based on the structure of their cell wall and response to the Gram stain. Gram-positive bacteria have a single plasma membrane and a thick peptidoglycan layer, while Gram-negative bacteria have two membranes (inner and outer) with a thin layer of peptidoglycan.

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    Peptidoglycan

    A complex polymer found exclusively in bacterial cell walls, composed of sugar and amino acids. It provides structural support and contributes to the rigidity of the cell wall.

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    Spirochete

    A specialized type of bacteria characterized by long, thin, flexible helical (spiral) shapes. Examples include Treponema pallidum, the bacterium responsible for syphilis.

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    Flagella

    A type of bacterial cell component involved in motility, allowing bacteria to move through their environment. It's a long, whip-like structure that spins, propelling the cell.

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    Peptidoglycan Layer

    A rigid layer surrounding bacterial cells made up of peptidoglycan, which controls the passage of small particles (up to 2nm).

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    Peptidoglycan Layer Thickness

    A significant difference in Gram-positive and Gram-negative bacteria is the thickness of their peptidoglycan layer. This layer makes up 90% of the cell weight in Gram-positive bacteria and only 10% in Gram-negative bacteria.

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    Plasma Membrane

    The outermost layer of a bacterial cell, composed of a phospholipid bilayer, embedded proteins, and lipopolysaccharides (in Gram-negative bacteria). It plays a crucial role in regulating the passage of substances into and out of the cell.

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    Amphipathic Phospholipids in Plasma Membrane

    The building blocks of the plasma membrane, consisting of a hydrophilic head (attracted to water) and a hydrophobic tail (repelled by water).

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    Membrane Proteins

    Membrane proteins are essential for various cellular functions including transport of ions and other molecules across the membrane. They can be integral, embedded in the phospholipid layer, or peripheral, loosely attached to the membrane surface.

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    Lipopolysaccharide (LPS)

    A complex molecule found in the outer membrane of Gram-negative bacteria. It is composed of three domains: O antigen, core antigen, and lipid A. It plays a vital role in the structural integrity of the cell but is also responsible for triggering immune responses.

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    Bacterial Motility

    The ability of bacteria to move using whip-like structures called flagella. The number and arrangement of flagella vary among species.

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    Monotrichous Flagella

    A single flagellum at one end of a bacterium.

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    What is a flagellum?

    A long, whip-like structure extending outward from the bacterial cell, powered by proton flow across the membrane.

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    What is Lophotrichous flagella?

    Arrangement of flagella where multiple flagella are present at one end of the bacterium.

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    What is Amphitrichous flagella?

    Arrangement of flagella where flagella are present at both ends of the bacterium.

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    What is Peritrichous flagella?

    Arrangement of flagella where multiple flagella are located all around the bacterium.

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    What are pili?

    Short, hair-like protein appendages found on the surface of bacteria, involved in adhesion and conjugation.

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    What is a capsule?

    A thick, protective layer of polysaccharide surrounding the bacterial cell, contributing to virulence and survival.

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    What is a nucleoid?

    The main component of bacterial genetic material, containing a single, circular chromosome of DNA.

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    What are plasmids?

    Small, circular pieces of DNA found independently in the cytoplasm of bacteria, often carrying genes for antibiotic resistance or virulence factors.

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    Sporulation

    A dormant, resistant state of a bacterial cell, formed in response to adverse environmental conditions like nutrient scarcity. This state enhances survival by providing resistance to temperature extremes, pH changes, and chemicals.

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    Hypha

    A long, filamentous structure characteristic of some bacteria, often containing a continuous cytoplasm and separated by cross-walls called septa. These filaments can form a dense network called a mycelial mat.

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    Trichomes (Cyanobacteria)

    They often form a long chain of cells and have a similar appearance to algae, although they are bacteria. Some cyanobacteria also fix nitrogen, making this a crucial process for life on earth.

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    Bacteriophages

    Viruses that infect bacteria, acting as parasites to their host. They can be categorized by their structure and are used as models for studying viral mechanisms.

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    Protein Coat (Capsid)

    A crucial component of a virus. Typically composed of proteins, this coat protects the genetic material and facilitates attachment to host cells.

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    Study Notes

    MPharm Programme: Microbial Structure

    • The presentation covers microbial structures, including bacteria and viruses.
    • Learning objectives include describing bacterial morphology and providing examples, internal and external bacterial structures, and viral morphology and structures.
    • Bacterial cell structure involves morphology (structure), colonies, individual cells, internal components, and external components, along with how these relate to physiology and pathogenicity.
    • Bacteria can form colonies in various shapes, such as punctiform, circular, rhizoid, irregular, filamentous, lobate, filamentous, and curled, depending on species. These can be used to classify bacteria although it is often inaccurate.
    • Bacteria come in diverse shapes, ranging in size from 1 to 10 µm.
    • Bacteria shapes include cocci (spheres), bacilli (rod-shaped), and spiral bacteria.
    • Cocci can be single (monococci), paired (diplococci), grouped (staphylococci), chained (streptococci), in groups of four (tetrads) or in cuboidal arrangements of eight (sarcina).
    • Bacilli can be single (bacillus), paired (diplobacilli), or chained (streptobacilli).
    • Spiral bacteria include spirillum and spirochete types.
    • Prokaryotic cells are simpler than eukaryotic cells. The presentation focuses on differences.
    • Bacterial cells have a cell wall (peptidoglycan), plasma membrane, genetic material (nucleoid and plasmids), protein production (ribosomes), motility (flagella), and pili. Special structures like endospores and capsules/slime layers are also discussed.
    • Gram-positive bacteria have a single plasma membrane; Gram-negative have two.
    • Peptidoglycan, a polymer of sugar and amino acids, is found in the cell wall, forming a layer that permits the passage of small particles (<2nm). Thickness varies between gram-positive and gram-negative bacteria. Gram-positive cell walls contain 90% peptidoglycan, gram-negative 10%.
    • Plasma membranes contain a lipid bilayer with proteins. Gram-negative bacteria have Lipopolysaccharide (LPS) which is crucial to their structural integrity. The LPS consists of o antigen, core antigen and lipid A. Small amounts of LPS can cause severe illness.
    • Cell motility involves, for example, flagella which are long and used for movement. They are powered by H+ flow. Flagella are also important for bacterial pathogenesis.
    • Pili or fimbriae are found mainly in Gram-negative bacteria and have various functions, including DNA transfer (conjugative pili) and twitching motility (type IV pili).
    • Capsules and slime layers are extracellular structures, often composed of polysaccharides. Capsules are organized and permanent, slime layers less organized and easily lost.
    • Bacterial internal components such as nucleoid (genetic material), plasmids (extra chromosomal DNA), and ribosomes (protein synthesis).
    • Endospores are a survival mechanism where cells are dormant, encased in a protective layer, resistant to heat, chemical treatments and radiation. Bacterial endospores can cause several diseases (e.g. Clostridium difficile diarrhea, anthrax).
    • Multicellular microorganisms, such as cyanobacteria and streptomycetes have structures like mycelia formed by hyphae (filaments of cells).
    • Viruses are simpler than bacterial cells, with genetic material (DNA or RNA), a protein coat, and, sometimes, a lipid envelope.
    • Bacteriophages are viruses that infect bacteria. They are a good model organism. Most bacteriophages belong to the Caudovirales family.

    Viral Structures

    • Viruses are simpler structures than bacteria or fungi.
    • Viruses have genetic material (DNA or RNA), a protein coat, receptors, and sometimes a lipid envelope.
    • Viruses can also infect prokaryotes like bacteria, such as bacteriophages.
    • Bacteriophages are non-pathogenic, and are often used as a model organism. Most bacteriophages (%95) are in the Caudovirales family.

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    Description

    Test your knowledge of bacterial cell structure and the functions of various components such as peptidoglycan and lipopolysaccharides. This quiz covers key aspects of Gram-positive and Gram-negative bacteria, membrane proteins, and the overall significance of these elements in bacterial physiology.

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