Bacterial and Eukaryotic Ribosomes Structure

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Questions and Answers

What is the function of the Peptidyl-transferase centre in ribosomes?

  • Match tRNAs to codons
  • Identify the start codon
  • Catalyze formation of peptide bonds (correct)
  • Facilitate complementary base pairing

Which RNA molecule is found in both bacterial and eukaryotic small ribosomal subunits?

  • 18S rRNA
  • 16S rRNA (correct)
  • 5S rRNA
  • 23S rRNA

In bacterial mRNA, what is the function of the Shine–Dalgarno sequence?

  • Separate translation segments
  • Facilitate complementary base pairing
  • Bind mRNA and identify the start codon (correct)
  • Catalyze formation of peptide bonds

What differentiates bacterial and eukaryotic translation initiation processes?

<p>Positioning of the ribosome on the start codon (C)</p> Signup and view all the answers

In translation, what does the Decoding centre of ribosomes specifically do?

<p>Match tRNAs to codons (D)</p> Signup and view all the answers

Which region of mRNA marks the boundaries of translation and contains the stop codon?

<p>3' untranslated region (3' UTR) (A)</p> Signup and view all the answers

What is needed for initiation of translation in bacteria?

<p>mRNA, small ribosomal subunit, large subunit, initiator tRNA, and initiation factor proteins (C)</p> Signup and view all the answers

What is the role of IF1 in the initiation of translation in bacteria?

<p>To prevent the initiator tRNA from going into the incorrect site (D)</p> Signup and view all the answers

Which sequence base-pairs with the 16S rRNA to position the start codon correctly in bacteria?

<p>Shine–Dalgarno sequence (D)</p> Signup and view all the answers

What is the name of the modified amino acid carried by the initiator tRNA during translation initiation in bacteria?

<p>N-formylmethionine (fMet) (A)</p> Signup and view all the answers

What happens when the 50S large subunit joins the 30S small subunit during translation initiation in bacteria?

<p>The union of the two subunits with dissociation of initiation factors (D)</p> Signup and view all the answers

What is necessary to guide the initiator tRNA into the P site during translation initiation in bacteria?

<p>IF2 (D)</p> Signup and view all the answers

What is the function of the Shine-Dalgarno sequence in prokaryotic mRNA?

<p>Finding and positioning the start codon on the ribosome (C)</p> Signup and view all the answers

What are some proteins involved in recognizing mRNA in eukaryotic translation initiation?

<p>eIF4E, eIF4G, and PABP (A)</p> Signup and view all the answers

Which release factor recognizes all three stop codons in eukaryotes and archaea?

<p>eRF1 (C)</p> Signup and view all the answers

What is the role of eIF1a during translation initiation in eukaryotes?

<p>Blocking the A site on the ribosome (A)</p> Signup and view all the answers

Why is the consensus Kozak sequence important in translation initiation?

<p>Locates the correct start codon on mRNA (C)</p> Signup and view all the answers

Which antibiotics block elongation step in protein synthesis?

<p>Erythromycin (D)</p> Signup and view all the answers

What occurs when a match is found during translation initiation?

<p>Initiation factor dissociation (A)</p> Signup and view all the answers

In bacteria, which component recognizes stop codons UAG and UAA?

<p>RF1 (D)</p> Signup and view all the answers

What does EF-G do during elongation phase of protein synthesis in bacteria?

<p>Translocates the ribosome along mRNA (C)</p> Signup and view all the answers

What blocks translocation step in protein synthesis?

<p>Cycloheximide (A)</p> Signup and view all the answers

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