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Bacteria Counting Methods in Liquids

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Questions and Answers

What is the purpose of sterilization validation?

To calculate the inactivation factor of a sterilization process

To verify the efficiency of sterilization methods under different conditions (correct)

To develop a new sterilization method

To compare the results of different sterilization methods

What is the unit of measurement for the inactivation factor (IF)?

Dimensionless (correct)

Percentage

Logarithmic scale

Ratio of initial to final viable count

What is the effect of increasing time on the log number of percentage survivors?

It becomes more negative (correct)

It becomes zero

It remains constant

It becomes more positive

What is the formula to calculate the percentage survivors?

(Number of survivors after sterilization / starting number of cells) * 100 Signup and view all the answers

What is the purpose of using biological indicator strips in sterilization methods?

To test the efficiency of sterilization methods Signup and view all the answers

What is the principle behind the turbidity method of counting bacteria in liquids?

Measuring the cloudiness of a liquid Signup and view all the answers

What is the formula to calculate the log of the percentage survivors?

log10%S = log (Nt/N0 x 100) Signup and view all the answers

What is the analogy used to describe the percentage survivors of a sterilization process?

A room with 20 people Signup and view all the answers

What is the unit of measurement for the initial population of bacteria?

Cells per mL Signup and view all the answers

What is the purpose of enumeration methods in bacterial counting?

To determine the number of bacteria present Signup and view all the answers