Bacteria and Archaea

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Questions and Answers

Given the divergent evolutionary pathways of Eubacteria and Archaebacteria, which statement most accurately reflects the phylogenetic implications of their classification as separate kingdoms, considering the endosymbiotic theory?

  • The distinction indicates that _Archaebacteria_ share a more recent common ancestor with _Eubacteria_ than with eukaryotes as they lack peptidoglycan.
  • The separate classification implies convergent evolution in response to similar environmental pressures, negating any deep phylogenetic significance.
  • The classification reflects fundamental differences in membrane lipid chirality and translational machinery, suggesting that _Archaebacteria_ represent a lineage that diverged prior to the evolutionary innovation of peptidoglycan synthesis by _Eubacteria_. (correct)
  • The separate classification suggests that _Eubacteria_ and _Archaebacteria_ diverged after the endosymbiotic event that gave rise to eukaryotic organelles, indicating a closer relationship between _Archaebacteria_ and eukaryotes.

In a hypothetical scenario where a novel antibiotic inhibits septum formation during binary fission, which of the following bacterial structures would be most likely to exhibit aberrant morphology or functionality as a direct consequence?

  • Endospores, preventing the bacteria from entering a state of dormancy during unfavorable conditions.
  • The cell wall, resulting in compromised structural integrity and osmoregulatory failure. (correct)
  • Plasmids, leading to impaired horizontal gene transfer capabilities.
  • Flagella, affecting motility due to disruptions in basal body assembly.

Considering the ecological roles of bacteria, particularly in nutrient cycling and decomposition, which statement best describes the impact of a broad-spectrum antibiotic on a soil ecosystem?

  • It would disrupt the carbon cycle by suppressing the decomposition activity of bacteria, resulting in an accumulation of organic matter. (correct)
  • It would have no significant impact, since soil bacteria rapidly develop resistance to most antibiotics.
  • It would promote the phosphorus cycle by inhibiting phosphate-solubilizing bacteria, leading to enhanced phosphorus uptake by plants.
  • It would enhance the nitrogen cycle by selectively inhibiting nitrogen-fixing bacteria, leading to an increased availability of atmospheric nitrogen.

In the context of bacterial genetic engineering, what critical advantage does a plasmid offer over the nucleoid as a target for modification and insertion of foreign genes?

<p>The nucleoid's compact structure inherently limits the size of inserts and accessibility to restriction enzymes, whereas plasmids provide more flexibility. (A)</p> Signup and view all the answers

Considering the Gram staining procedure, which aspect of the bacterial cell wall is most critically responsible for the differential staining observed between Gram-positive and Gram-negative bacteria?

<p>The thickness of the peptidoglycan layer determines the degree of crystal violet-iodine complex retention. (C)</p> Signup and view all the answers

Given the asexual nature of binary fission and the lack of meiotic recombination in bacteria, what mechanism contributes most significantly to genetic diversity within a bacterial population, thereby enabling adaptation to changing environmental conditions?

<p>Mutation, resulting from errors during DNA replication and repair. (A)</p> Signup and view all the answers

In the context of Plasmodium vivax lifecycle, what is the significance of the schizont stage within human hepatocytes, considering its contribution to pathogenesis and potential therapeutic targets?

<p>Schizonts are responsible for the asymptomatic liver stage prior to red blood cell invasion, presenting a target for prophylactic interventions. (B)</p> Signup and view all the answers

During the erythrocytic stage of Plasmodium vivax infection, what is the underlying mechanism for the cyclical fever patterns characteristic of malaria, considering the parasite's developmental stages and interactions with red blood cells?

<p>The periodic release of merozoites from infected erythrocytes triggers a systemic inflammatory response, leading to synchronized episodes of high fever. (B)</p> Signup and view all the answers

Considering the unique metabolic capabilities of Archaea such as methanogenesis, what ecological role do these organisms play in anaerobic environments like wetlands and the deep sea, and what impact do they have on global biogeochemical cycles?

<p>They serve as terminal electron acceptors in anaerobic respiration, converting organic matter into methane, a potent greenhouse gas. (C)</p> Signup and view all the answers

In the context of bacterial pathogenesis, how does the presence of a capsule contribute to the virulence of certain bacterial species, and what specific mechanisms underlie this enhanced pathogenicity?

<p>The capsule inhibits phagocytosis by interfering with opsonization and complement activation. (D)</p> Signup and view all the answers

Given that Clostridium botulinum is a superbug that produces deadly toxic chemicals, what is the most critical mechanism by which botulinum toxin exerts its paralytic effects on the human nervous system, and how does this mechanism relate to its clinical use in controlled doses?

<p>It blocks the release of acetylcholine at the neuromuscular junction. (B)</p> Signup and view all the answers

Considering the role of Streptococcus pyogenes as a causative agent of sore throats and other infections, what is the primary mechanism by which this bacterium evades the host's immune defenses during initial colonization of the pharyngeal mucosa?

<p>It produces a capsule composed of hyaluronic acid that mimics host tissue, preventing recognition by phagocytes. (D)</p> Signup and view all the answers

Considering the process of conjugation in bacteria as it relates to antibiotic resistance, under what conditions would this mechanism of horizontal gene transfer be most likely to confer a selective advantage to a bacterial population, leading to widespread antibiotic resistance?

<p>In an environment where antibiotics are present, conjugation allows for the rapid dissemination of resistance genes among bacteria, leading to increased survival rates and a shift in population dynamics. (C)</p> Signup and view all the answers

What is the primary mechanism by which bacterial endospores confer resistance to harsh environmental conditions, such as extreme heat, radiation, and desiccation, and how does this relate to their persistence and potential clinical significance?

<p>Endospores contain high concentrations of dipicolinic acid complexed with calcium ions, stabilizing DNA and proteins. (D)</p> Signup and view all the answers

Considering the role of bacterial nutrition, which of the following is a true statement?

<p>Bacteria may obtain energy through cellular respiration. (D)</p> Signup and view all the answers

Considering the diverse habitats of bacteria and archaea, what fundamental physiological adaptation enables extremophiles to thrive in conditions that would be lethal to mesophiles, and how does this relate to their unique enzymatic and membrane characteristics?

<p>Extremophiles synthesize unique membrane lipids with ether linkages and branched isoprenoids, providing increased stability at high temperatures and extreme pH levels. (D)</p> Signup and view all the answers

Considering the role of cyanobacteria in the environment, especially in aquatic ecosystems, what is the most critical contribution of these organisms to the oxygen cycle and the maintenance of life on Earth?

<p>Cyanobacteria release oxygen as a byproduct of photosynthesis. (C)</p> Signup and view all the answers

Which statement best describes the basis for classifying organisms into Archaea as opposed to Bacteria?

<p>Archaea are prokaryotic organisms lacking peptidoglycan in their cell walls, differentiating them from Bacteria, and display unique metabolic pathways such as methanogenesis. (A)</p> Signup and view all the answers

In the context of classifying bacteria and archaea, what is the primary rationale for comparing their morphology, nutrition, habitats, and reproduction?

<p>To identify key differences and similarities that reflect their evolutionary adaptations and ecological roles. (D)</p> Signup and view all the answers

In the lifecycle of Plasmodium vivax, how does the transition from the asexual cycle in humans to the sexual cycle in mosquitoes contribute to the parasite's overall survival and transmission strategy, and what specific molecular mechanisms facilitate this transition?

<p>The switch to sexual reproduction in mosquitoes enables genetic recombination, generating novel antigenic variants that evade the host's immune responses and prevent the development of long-term immunity. (D)</p> Signup and view all the answers

Considering that plasmids are crucial for horizontal gene transfer, what specific structural and functional characteristics distinguish them from the bacterial chromosome (nucleoid), and how do these differences impact their role in bacterial adaptation and evolution?

<p>Plasmids replicate independently of the bacterial chromosome. (C)</p> Signup and view all the answers

What mechanisms enable the bacterium Clostridium botulinum to persist in varied environments during periods unsuitable for active growth?

<p>Development of endospores capable of surviving very harsh conditions. (B)</p> Signup and view all the answers

Given that Streptococcus pyogenes is clinically significant, what virulence factor is most directly implicated in the bacterium's ability to cause invasive infections such as necrotizing fasciitis, undermining tissue integrity and facilitating bacterial dissemination?

<p>Hyaluronidase (A)</p> Signup and view all the answers

What distinguishes the cell walls of Gram-positive bacteria from Gram-negative bacteria?

<p>The thickness of the peptidoglycan layer and its structural rigidity. (C)</p> Signup and view all the answers

Cyanobacteria are classified as decomposers, responsible for the breakdown of organic matter?

<p>No, because cyanobacteria have another function. (A)</p> Signup and view all the answers

Which characteristic is exclusive to archaea, differentiating them from bacteria and eukaryotes?

<p>Cell walls lacking peptidoglycan. (D)</p> Signup and view all the answers

What adaptation allows bacteria to survive adverse environmental conditions by entering a dormant state?

<p>Development of endospores. (C)</p> Signup and view all the answers

How do bacteria contribute to the environment functioning, particularly in ecosystems?

<p>By facilitating the decomposition of organic material and nutrient cycling. (A)</p> Signup and view all the answers

What role do plasmids play in the genetic engineering of bacteria, and why are they preferred over the bacterial chromosome for these purposes?

<p>Plasmids replicate independently of the bacterial chromosome and allow for the relatively easy introduction and manipulation of genes. (D)</p> Signup and view all the answers

During Gram staining, what structural property of bacterial cell walls causes Gram-positive bacteria to stain differently from Gram-negative bacteria.

<p>The thickness of the peptidoglycan layer. (C)</p> Signup and view all the answers

How does Clostridium botulinum, a superbug, harm human health?

<p>Producing toxic chemicals that are deadly to humans. (B)</p> Signup and view all the answers

Considering Streptococcus pyogenes, what symptoms are caused to an infected individual?

<p>Sore throats. (C)</p> Signup and view all the answers

What occurs during the schizont stage of Plasmodium vivax infection?

<p>Multiplication of the parasite within the parasite-converted cell. (C)</p> Signup and view all the answers

What is the defining characterization of an extremophile versus a mesophile?

<p>Thriving in conditions lethal to mesophiles. (D)</p> Signup and view all the answers

What is the role of bacterial capsule?

<p>Confer resistance to complement-mediated killing. (C)</p> Signup and view all the answers

What are the three main shapes of bacteria as mentioned in the text?

<p>Spherical, rod shaped, and spiral. (C)</p> Signup and view all the answers

Which process is involved in the sexual reproduction of bacteria?

<p>Conjugation. (D)</p> Signup and view all the answers

Flashcards

Eubacteria

A kingdom that includes bacteria.

Archaebacteria

A kingdom that includes archaea.

Capsule (bacteria)

A structure that is outside the cell providing a protective layer

Flagella

A whip-like appendage used for movement.

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Cell Wall

The outer boundary of the cell, providing structure and protection.

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Cytoplasm

The gel-like substance inside the cell.

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Nucleoid Region

The area in a bacterial cell where the genetic material is located.

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Plasmid

Small, circular DNA molecules separate from the chromosome within a bacteria that can replicate independently.

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Coccus/Cocci

Spherical bacterial shape.

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Bacillus/Bacilli

Rod-shaped bacterial shape.

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Binary Fission

Asexual reproduction where a cell divides into two identical cells.

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Conjugation (bacteria)

Reproductive process of exchanging genetic material (plasmids).

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Gram Stains

Stains that divide bacteria into Gram-positive (purple) and Gram-negative (pink) based on protein layer thickness.

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Endospores

Hard structures that protect and store a dormant bacterial cell.

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Clostridium botulinum

Resistant to antibiotics, produces toxic chemicals deadly to humans.

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Streptococcus pyogenes

A gram-positive bacteria that causes sore throats.

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Decomposers

Organisms that break down organic material.

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Anopheles mosquito

Female mosquito that transmits Plasmodium vivax

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Within Man

Asexual cycle of Plasmodium vivax

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Within Mosquito

Sexual cycle of Plasmodium vivax

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Sporozoite

Parasitic cells injected by mosquitoes that travel to the liver

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Merozoites

Develop within the liver and are released into the bloodstream

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Rings

Formed when red blood cells are infected

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Trophozoites

Turn into schizont with many merozoites

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Schizont

Contain many merozoites inside by binary fission

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Gametocytes

Sexual cells created by schizont

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Zygotes

Fuse to create which develop in gut wall of mosquito

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Study Notes

  • Bacteria are classified under the Kingdom Eubacteria.
  • Archaea are classified under the Kingdom Archaebacteria.
  • Bacteria have a capsule and flagella.
  • Bacteria and Archaea both exist in spherical (cocci/coccus), rod-shaped (bacilli/bacillus), and spiral shapes.
  • Bacteria can be useful, beneficial, or harmful; E. coli produces vitamin K.
  • Archaea's cell walls do not contain peptidoglycan.
  • Classification of bacteria can be done by comparing morphology (shape/size/form), nutrition (inorganic compounds/photosynthesis/respiration), and habitat (aerobic/anaerobic, extremophile vs. mesophile).
  • Reproduction happens through binary fission, an asexual cloning process, or conjugation, a sexual process of exchanging plasmids.
  • Gram stains differentiate bacteria into Gram-positive (purple) or Gram-negative (pink), based on protein layer thickness.
  • Plasmids are small, self-replicating DNA loops within bacteria.
  • Endospores are protective structures for dormant bacteria cells.
  • Clostridium botulinum is a superbug that produces deadly toxins.
  • Streptococcus pyogenes is a Gram-positive bacterium causing sore throats of humans.
  • Bacteria serve as decomposers in the food chain and can perform photosynthesis to produce oxygen.
  • Plasmids are easier to engineer compared to nucleoids.
  • The asexual cycle of Plasmodium vivax happens within humans.
  • The sexual cycle of Plasmodium vivax happens within female Anopheles mosquitoes.

Asexual Reproduction of Plasmodium Vivax

  • Mosquitoes inject sporozoites that migrate to the liver.
  • Merozoites develop in the liver, and are released into the bloodstream.
  • Red blood cells get infected, forming rings.
  • Rings transform into trophozoites, absorbing nutrients and growing.
  • Trophozoites become schizonts, containing many merozoites, they divide by binary fission.
  • Schizonts rupture, releasing merozoites.

Sexual Reproduction of Plasmodium Vivax

  • Some schizonts turn into gametocytes, sexual cells.
  • Mosquitoes ingest human blood containing gametocytes.
  • Gametocytes merge to create zygotes in the mosquito's gut wall.
  • Zygotes rupture, releasing sporozoites that migrate to the salivary gland.

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