BACTE211 - Clinical Bacteriology Midterms: Vibrio, Aeromonas, Plesiomonas, Helicobacter
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Questions and Answers

Which type of bacteria are Campylobacter C.jejuni and C.coli?

  • Curved or S-shaped gram-negative rods (correct)
  • Gram-positive cocci
  • Cocci arranged in clusters
  • Bacilli

    • What is a common characteristic of Campylobacter species in terms of oxygen requirements?

  • Anaerobic
  • Microaerophilic (correct)
  • Strictly aerobic
  • Facultative anaerobic

    • Which of the following tests is NOT typically used to identify Campylobacter species?

  • Catalase test
  • Oxidase test
  • Gram stain
  • TSI test (correct)

    • What is a selective medium used for isolating Campylobacter from stool specimens?

    <p>Brucella agar with antimicrobials (C)</p> Signup and view all the answers

    Which of the following is a characteristic shared by Campylobacter C.jejuni and C.coli?

    <p>Oxidase-positive (A)</p> Signup and view all the answers

    At what temperature can some species of Campylobacter be selectively isolated?

    <p>42 degrees Celsius (C)</p> Signup and view all the answers

    What is the most common cause of bacterial gastroenteritis worldwide?

    <p>Campylobacter spp. (D)</p> Signup and view all the answers

    Which type of medium is commonly used to transport feces and blood specimens for Campylobacter and Arcobacter?

    <p>Cary-Blair transport medium (B)</p> Signup and view all the answers

    What is the typical colony morphology of C.jejuni and other enteric campylobacters?

    <p>Moist, runny looking, and spreading (D)</p> Signup and view all the answers

    How are Campylobacter spp. described in terms of their shape?

    <p>Curved rods (A)</p> Signup and view all the answers

    Which of the following animals are primarily affected by Campylobacters in terms of causing abortion?

    <p>Cattle, sheep, and swine (A)</p> Signup and view all the answers

    What is the method used to detect both C.jejuni and C.coli Campylobacter spp.?

    <p>Enzyme immunoassays (EIA) (B)</p> Signup and view all the answers

    What type of flagella does Vibrio cholerae produce when grown in broth?

    <p>Polar, sheathed flagella (B)</p> Signup and view all the answers

    Which of the following is a characteristic of V. cholerae O139?

    <p>Gives positive results on the Voges-Proskauer test (B)</p> Signup and view all the answers

    In which media can V. cholerae produce peritrichous, unsheathed flagella?

    <p>Solid media (A)</p> Signup and view all the answers

    Which biotype of epidemic V. cholerae produces a hemolysin?

    <p>El Tor (D)</p> Signup and view all the answers

    What is the name of the heat-stable hemolysin produced by Vibrio parahaemolyticus?

    <p>Kanagawa phenomenon (B)</p> Signup and view all the answers

    Which Vibrio species requires 1% to 8% NaCl for growth?

    <p>V. parahaemolyticus (D)</p> Signup and view all the answers

    What is a key virulence factor of H.pylori?

    <p>Urease activity (C)</p> Signup and view all the answers

    How is H.pylori presumptively identified in a gastric biopsy specimen?

    <p>Rapid urease reaction testing (B)</p> Signup and view all the answers

    Which test is recommended for monitoring therapy of H.pylori infection?

    <p>Urea breath test (C)</p> Signup and view all the answers

    How is urease activity detected in the urea breath test?

    <p>Detection of 13CO2 or 14CO2 in exhaled breath (C)</p> Signup and view all the answers

    What staining technique can be used on biopsy samples to aid in the identification of H.pylori?

    <p>Warthin-Starry or Giemsa stains (D)</p> Signup and view all the answers

    Which gene is NOT an amplification target for detecting H.pylori?

    <p>toxA gene (B)</p> Signup and view all the answers

    What is the recommended medium for transporting stool specimens suspected of containing Vibrio spp?

    <p>Cary Blair medium (B)</p> Signup and view all the answers

    Which Vibrio species is associated with recent consumption of raw seafood, especially oysters?

    <p>Vibrio parahaemolyticus (A)</p> Signup and view all the answers

    What is the optimal pH range for taurocholate peptone broth used for enriching Vibrio spp. from stool specimens?

    <p>8.0-9.0 (B)</p> Signup and view all the answers

    What inhibits the growth of gram-positive organisms in Thiosulfate citrate bile salt sucrose (TCBS) agar?

    <p>Bile salts (C)</p> Signup and view all the answers

    What is the characteristic color of sucrose-fermenting colonies of some Vibrio species?

    <p>Yellow (C)</p> Signup and view all the answers

    What is the recommended incubation time for stool specimens in taurocholate peptone broth before subculturing to TCBS agar?

    <p>5-8 hours (B)</p> Signup and view all the answers

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