BACTE211 - Clinical Bacteriology Midterms: Vibrio, Aeromonas, Plesiomonas, Helicobacter

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Questions and Answers

Which type of bacteria are Campylobacter C.jejuni and C.coli?

  • Curved or S-shaped gram-negative rods (correct)
  • Gram-positive cocci
  • Cocci arranged in clusters
  • Bacilli

What is a common characteristic of Campylobacter species in terms of oxygen requirements?

  • Anaerobic
  • Microaerophilic (correct)
  • Strictly aerobic
  • Facultative anaerobic

Which of the following tests is NOT typically used to identify Campylobacter species?

  • Catalase test
  • Oxidase test
  • Gram stain
  • TSI test (correct)

What is a selective medium used for isolating Campylobacter from stool specimens?

<p>Brucella agar with antimicrobials (C)</p> Signup and view all the answers

Which of the following is a characteristic shared by Campylobacter C.jejuni and C.coli?

<p>Oxidase-positive (A)</p> Signup and view all the answers

At what temperature can some species of Campylobacter be selectively isolated?

<p>42 degrees Celsius (C)</p> Signup and view all the answers

What is the most common cause of bacterial gastroenteritis worldwide?

<p>Campylobacter spp. (D)</p> Signup and view all the answers

Which type of medium is commonly used to transport feces and blood specimens for Campylobacter and Arcobacter?

<p>Cary-Blair transport medium (B)</p> Signup and view all the answers

What is the typical colony morphology of C.jejuni and other enteric campylobacters?

<p>Moist, runny looking, and spreading (D)</p> Signup and view all the answers

How are Campylobacter spp. described in terms of their shape?

<p>Curved rods (A)</p> Signup and view all the answers

Which of the following animals are primarily affected by Campylobacters in terms of causing abortion?

<p>Cattle, sheep, and swine (A)</p> Signup and view all the answers

What is the method used to detect both C.jejuni and C.coli Campylobacter spp.?

<p>Enzyme immunoassays (EIA) (B)</p> Signup and view all the answers

What type of flagella does Vibrio cholerae produce when grown in broth?

<p>Polar, sheathed flagella (B)</p> Signup and view all the answers

Which of the following is a characteristic of V. cholerae O139?

<p>Gives positive results on the Voges-Proskauer test (B)</p> Signup and view all the answers

In which media can V. cholerae produce peritrichous, unsheathed flagella?

<p>Solid media (A)</p> Signup and view all the answers

Which biotype of epidemic V. cholerae produces a hemolysin?

<p>El Tor (D)</p> Signup and view all the answers

What is the name of the heat-stable hemolysin produced by Vibrio parahaemolyticus?

<p>Kanagawa phenomenon (B)</p> Signup and view all the answers

Which Vibrio species requires 1% to 8% NaCl for growth?

<p>V. parahaemolyticus (D)</p> Signup and view all the answers

What is a key virulence factor of H.pylori?

<p>Urease activity (C)</p> Signup and view all the answers

How is H.pylori presumptively identified in a gastric biopsy specimen?

<p>Rapid urease reaction testing (B)</p> Signup and view all the answers

Which test is recommended for monitoring therapy of H.pylori infection?

<p>Urea breath test (C)</p> Signup and view all the answers

How is urease activity detected in the urea breath test?

<p>Detection of 13CO2 or 14CO2 in exhaled breath (C)</p> Signup and view all the answers

What staining technique can be used on biopsy samples to aid in the identification of H.pylori?

<p>Warthin-Starry or Giemsa stains (D)</p> Signup and view all the answers

Which gene is NOT an amplification target for detecting H.pylori?

<p>toxA gene (B)</p> Signup and view all the answers

What is the recommended medium for transporting stool specimens suspected of containing Vibrio spp?

<p>Cary Blair medium (B)</p> Signup and view all the answers

Which Vibrio species is associated with recent consumption of raw seafood, especially oysters?

<p>Vibrio parahaemolyticus (A)</p> Signup and view all the answers

What is the optimal pH range for taurocholate peptone broth used for enriching Vibrio spp. from stool specimens?

<p>8.0-9.0 (B)</p> Signup and view all the answers

What inhibits the growth of gram-positive organisms in Thiosulfate citrate bile salt sucrose (TCBS) agar?

<p>Bile salts (C)</p> Signup and view all the answers

What is the characteristic color of sucrose-fermenting colonies of some Vibrio species?

<p>Yellow (C)</p> Signup and view all the answers

What is the recommended incubation time for stool specimens in taurocholate peptone broth before subculturing to TCBS agar?

<p>5-8 hours (B)</p> Signup and view all the answers

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